##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930929_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1753060 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.539108187968466 30.0 16.0 31.0 2.0 33.0 2 22.36408223335197 30.0 10.0 31.0 2.0 33.0 3 22.87159652265182 30.0 16.0 31.0 2.0 34.0 4 24.834481991489167 33.0 10.0 35.0 2.0 37.0 5 24.823956966675414 33.0 10.0 35.0 2.0 37.0 6 25.623324928981326 33.0 17.0 35.0 2.0 37.0 7 25.742886153354707 33.0 17.0 35.0 2.0 37.0 8 26.599488893705864 33.0 17.0 35.0 2.0 37.0 9 27.868572667221887 35.0 18.0 38.0 2.0 39.0 10-11 28.18252370141353 35.0 25.0 38.0 2.0 39.0 12-13 28.158933807171458 35.0 25.0 38.0 2.0 39.0 14-15 28.452092911822756 35.5 21.5 38.5 2.0 40.0 16-17 27.552808232462095 34.0 17.0 38.0 2.0 40.0 18-19 26.906021471027803 34.0 13.5 38.0 2.0 40.0 20-21 26.357787811027578 34.0 10.0 38.0 2.0 40.0 22-23 26.33507124684837 33.0 11.0 38.0 2.0 40.0 24-25 26.04239501215018 33.0 10.0 38.0 2.0 40.0 26-27 25.72456818363319 32.5 10.0 38.0 2.0 40.0 28-29 25.706487513262523 33.0 9.5 38.0 2.0 40.0 30-31 25.681158374499446 33.0 9.0 38.0 2.0 40.0 32-33 25.436161340741332 33.0 8.5 38.0 2.0 40.0 34-35 22.339283310325946 29.0 2.0 37.0 2.0 39.0 36-37 20.66170239466989 22.5 2.0 36.0 2.0 39.0 38-39 19.676299156902786 20.0 2.0 35.0 2.0 39.0 40-41 21.16058064184911 25.5 2.0 35.5 2.0 39.0 42-43 21.100450925809724 25.5 2.0 36.0 2.0 39.0 44-45 19.023381686878942 19.5 2.0 34.5 2.0 38.5 46-47 19.975993405816116 20.0 2.0 34.0 2.0 38.0 48-49 19.395914857449263 22.5 2.0 35.0 2.0 38.0 50-51 17.2320091725326 12.0 2.0 33.0 2.0 37.5 52-53 20.331783852235517 24.0 2.0 34.0 2.0 38.0 54-55 22.16605906243939 29.0 2.0 36.5 2.0 39.0 56-57 22.433167432945822 30.0 2.0 37.0 2.0 39.0 58-59 22.061554938222308 29.0 2.0 36.0 2.0 39.0 60-61 21.741962910567807 28.0 2.0 36.0 2.0 39.0 62-63 21.26021670678699 28.0 2.0 35.5 2.0 39.0 64-65 20.746971295905446 27.5 2.0 35.0 2.0 38.0 66-67 20.321636167615484 26.0 2.0 34.5 2.0 38.0 68-69 19.858466624074474 26.0 2.0 34.0 2.0 37.5 70-71 19.25007843428063 25.5 2.0 34.0 2.0 37.0 72-73 18.778464228263722 24.0 2.0 34.0 2.0 36.0 74-75 18.233351396985842 22.0 2.0 33.0 2.0 36.0 76-77 17.65933881327507 20.5 2.0 32.5 2.0 35.0 78-79 17.152024460086935 19.0 2.0 32.0 2.0 35.0 80-81 16.64008647735959 17.0 2.0 32.0 2.0 35.0 82-83 16.162168151689045 10.0 2.0 31.0 2.0 34.0 84-85 15.638635015344597 7.0 2.0 31.0 2.0 34.0 86-87 15.069572632996017 2.0 2.0 31.0 2.0 34.0 88-89 14.502212702360444 2.0 2.0 30.5 2.0 34.0 90-91 13.908471757954661 2.0 2.0 30.0 2.0 34.0 92-93 13.26279220334729 2.0 2.0 29.0 2.0 34.0 94-95 12.580900539627851 2.0 2.0 29.0 2.0 34.0 96-97 11.804821854357524 2.0 2.0 29.0 2.0 34.0 98-99 10.94260378994444 2.0 2.0 26.5 2.0 33.5 100-101 9.757504877186177 2.0 2.0 22.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.43905357600762 1101 2 7.784541589815966 1101 3 7.2163785473137985 1101 4 9.221323052832723 1101 5 9.059971237684014 1101 6 8.370369682237904 1101 7 8.147570337600744 1101 8 7.170394311428016 1101 9 7.250738567897262 1101 10-11 6.76596702127954 1101 12-13 6.43270507129472 1101 14-15 7.0161832421808725 1101 16-17 7.741202066191573 1101 18-19 8.271801261453149 1101 20-21 8.803322358786492 1101 22-23 8.681581226038237 1101 24-25 8.717968316723127 1101 26-27 8.519553413513915 1101 28-29 8.605123458124112 1101 30-31 8.218659623170527 1101 32-33 8.132421173296503 1101 34-35 10.693223165035906 1101 36-37 4.600086631932729 1101 38-39 7.974253282667647 1101 40-41 9.678420785055621 1101 42-43 9.886591085626122 1101 44-45 4.075720139267563 1101 46-47 5.962940788193144 1101 48-49 9.262916812485468 1101 50-51 -4.280981055941115 1101 52-53 5.594752659986444 1101 54-55 8.030170274647038 1101 56-57 8.513189590421486 1101 58-59 8.974952568420349 1101 60-61 8.747778709698704 1101 62-63 8.853575315723216 1101 64-65 8.651352668502586 1101 66-67 8.481563034586216 1101 68-69 8.623931138705231 1101 70-71 8.71563803684884 1101 72-73 8.73439029383939 1101 74-75 8.885400695017289 1101 76-77 8.611671402075064 1101 78-79 8.441389019914904 1101 80-81 8.411824523725123 1101 82-83 8.148278207971076 1101 84-85 8.121088981907763 1101 86-87 8.12752010183084 1101 88-89 8.164680371280914 1101 90-91 8.049391315775473 1101 92-93 7.960203888859114 1101 94-95 7.437991465305199 1101 96-97 7.123233371085606 1101 98-99 6.615171342159149 1101 100-101 6.286298146195092 1102 1 6.564348563105849 1102 2 7.645662791309064 1102 3 7.202949431899313 1102 4 9.052180206426435 1102 5 9.203138935657304 1102 6 8.520429806996777 1102 7 8.293469328005838 1102 8 7.202189035480426 1102 9 7.278029571730212 1102 10-11 6.617836525500795 1102 12-13 6.356692971186995 1102 14-15 6.69181361269969 1102 16-17 7.486873083552606 1102 18-19 7.769064019175417 1102 20-21 8.336873539987483 1102 22-23 8.174353415832062 1102 24-25 8.559357010763254 1102 26-27 8.550907848967487 1102 28-29 8.237866399727949 1102 30-31 8.063967073630227 1102 32-33 7.91649218314193 1102 34-35 10.423980110698164 1102 36-37 0.7078978401898652 1102 38-39 3.3163428059284144 1102 40-41 8.41213198579716 1102 42-43 9.682346966579097 1102 44-45 0.9232738131408524 1102 46-47 1.4229515605457763 1102 48-49 7.687969108499697 1102 50-51 -5.4001859060049995 1102 52-53 4.861803978973345 1102 54-55 7.678638032745091 1102 56-57 8.13565151140753 1102 58-59 8.458867316522664 1102 60-61 8.089689424431771 1102 62-63 8.235795610334534 1102 64-65 8.238822919272934 1102 66-67 8.379127355552221 1102 68-69 8.14462783434869 1102 70-71 8.1134503724452 1102 72-73 8.179441587774306 1102 74-75 7.988445763534465 1102 76-77 7.940206550007023 1102 78-79 7.986627890571519 1102 80-81 8.06471708829661 1102 82-83 7.657617900332729 1102 84-85 7.621400565944638 1102 86-87 7.409869727303693 1102 88-89 7.351614822767749 1102 90-91 7.261573781892665 1102 92-93 7.3080869963070665 1102 94-95 6.799644708284379 1102 96-97 6.828477690548491 1102 98-99 6.787235249767432 1102 100-101 6.244646719484667 1103 1 6.708682579279017 1103 2 7.922735036173176 1103 3 7.533921695498012 1103 4 9.19339568180888 1103 5 9.175552718170255 1103 6 8.509115038307488 1103 7 8.19517632522022 1103 8 7.321570732711084 1103 9 7.463472798255932 1103 10-11 6.822190627607366 1103 12-13 6.833460241557788 1103 14-15 7.357830916086357 1103 16-17 7.897083433029412 1103 18-19 8.309735270185087 1103 20-21 8.65722390746178 1103 22-23 8.636013852992878 1103 24-25 8.934570999663787 1103 26-27 8.676357095130736 1103 28-29 8.802882784812752 1103 30-31 8.408462790780977 1103 32-33 8.36318430018088 1103 34-35 10.870648732723279 1103 36-37 -3.224828871337433 1103 38-39 -1.6752419865015824 1103 40-41 7.258272968165148 1103 42-43 9.636325916275197 1103 44-45 0.893040797885984 1103 46-47 0.6436879833454583 1103 48-49 8.239600553120564 1103 50-51 -5.673475453641524 1103 52-53 4.712607262747042 1103 54-55 8.036218015607552 1103 56-57 8.571164608726026 1103 58-59 8.875745950187792 1103 60-61 8.677612526633549 1103 62-63 8.70977849122249 1103 64-65 8.84243854084658 1103 66-67 8.737693207622698 1103 68-69 8.746814406257254 1103 70-71 8.737485464475018 1103 72-73 8.507155876121086 1103 74-75 8.477005380980355 1103 76-77 8.473979173555477 1103 78-79 8.412684168039952 1103 80-81 8.063255637011142 1103 82-83 7.899481408122936 1103 84-85 7.963540946348877 1103 86-87 7.512869564527783 1103 88-89 7.655421015009173 1103 90-91 7.593163672587107 1103 92-93 7.6860492875776725 1103 94-95 7.45064270890477 1103 96-97 7.510310209490386 1103 98-99 7.176130093456488 1103 100-101 6.478485989020079 1104 1 6.574226605669502 1104 2 7.619409106647154 1104 3 7.047653264547442 1104 4 9.105516118648268 1104 5 8.934845470140946 1104 6 7.979691765092834 1104 7 8.010924804695382 1104 8 7.238751077240426 1104 9 7.193950309892063 1104 10-11 6.53908086918867 1104 12-13 6.330641099543669 1104 14-15 7.073473517280817 1104 16-17 7.559088858553576 1104 18-19 8.081733858474678 1104 20-21 8.258141873335813 1104 22-23 8.23073271635354 1104 24-25 8.318681791782634 1104 26-27 8.33363739253421 1104 28-29 8.318683967356293 1104 30-31 8.031947024271506 1104 32-33 7.810358326833995 1104 34-35 10.557585276871531 1104 36-37 -3.458055883755115 1104 38-39 -2.2778151120089554 1104 40-41 6.221237111487987 1104 42-43 8.764953863006738 1104 44-45 0.2502204991191981 1104 46-47 -0.6663199634273944 1104 48-49 7.161972563010803 1104 50-51 -6.046257299234519 1104 52-53 3.952797660696522 1104 54-55 6.908531848510496 1104 56-57 7.606841428553242 1104 58-59 7.8144036803498516 1104 60-61 7.501678707050964 1104 62-63 7.775141952061251 1104 64-65 7.77388515744828 1104 66-67 7.605697103990643 1104 68-69 7.483496332026192 1104 70-71 7.459522665286986 1104 72-73 7.2099855233380765 1104 74-75 7.217356602962667 1104 76-77 7.071114528668222 1104 78-79 6.657593054585828 1104 80-81 6.5752292075732015 1104 82-83 6.541570916582723 1104 84-85 6.4719622238700705 1104 86-87 6.1351744709591784 1104 88-89 6.192851849609179 1104 90-91 6.110692311806275 1104 92-93 6.364493585067313 1104 94-95 6.194467139816389 1104 96-97 5.534809611490568 1104 98-99 5.184419613306748 1104 100-101 5.036080911347975 1105 1 5.986101747046703 1105 2 7.238621363617781 1105 3 6.813559790161488 1105 4 8.74510127683773 1105 5 8.502266272457081 1105 6 7.728876960573 1105 7 7.499977097744189 1105 8 6.427264113683787 1105 9 6.520418323542064 1105 10-11 6.22767154691557 1105 12-13 5.6756695265484325 1105 14-15 6.067123375396022 1105 16-17 6.697324802836018 1105 18-19 7.55486211015026 1105 20-21 7.894493121930619 1105 22-23 7.6867567219517134 1105 24-25 7.786071308808101 1105 26-27 7.6071553376797105 1105 28-29 7.288902315235575 1105 30-31 7.356142421787727 1105 32-33 7.178931651805524 1105 34-35 9.510272423106823 1105 36-37 -3.9673230787256735 1105 38-39 -2.6799695074922028 1105 40-41 5.667801320257535 1105 42-43 7.9204200238468765 1105 44-45 -3.6355869030263346 1105 46-47 -4.908929814404743 1105 48-49 5.050117639824197 1105 50-51 -10.556467771456525 1105 52-53 0.2788703186393384 1105 54-55 5.242204344135846 1105 56-57 6.2931341816616815 1105 58-59 6.653276855100508 1105 60-61 6.701994362479233 1105 62-63 6.7336776460327705 1105 64-65 6.72759779776381 1105 66-67 6.434105434800276 1105 68-69 6.196376434724307 1105 70-71 6.091798532917604 1105 72-73 6.154048338711107 1105 74-75 6.088909179838264 1105 76-77 6.083145924915872 1105 78-79 5.865684420217363 1105 80-81 5.721629640419785 1105 82-83 5.599998913657805 1105 84-85 5.514879551488553 1105 86-87 5.383801592904039 1105 88-89 4.999920838250672 1105 90-91 5.135567406570104 1105 92-93 5.1599997418924115 1105 94-95 4.862775283343236 1105 96-97 4.779069089518083 1105 98-99 4.648023295591182 1105 100-101 4.021716987920868 1106 1 5.990932054950623 1106 2 7.247004771628685 1106 3 6.875888159114854 1106 4 8.26028450945038 1106 5 8.317691034748176 1106 6 7.444741218322729 1106 7 7.2398754867369455 1106 8 6.561450347206279 1106 9 6.706227633647028 1106 10-11 6.1053378360284505 1106 12-13 5.944323087861406 1106 14-15 6.303238418367165 1106 16-17 6.839400529746083 1106 18-19 7.598398870224592 1106 20-21 7.73979114898594 1106 22-23 7.478948500230043 1106 24-25 7.642717802771283 1106 26-27 7.425548015516835 1106 28-29 7.459403985661112 1106 30-31 6.905981092531778 1106 32-33 6.8800490300872745 1106 34-35 9.876060479742744 1106 36-37 -0.06478851684279974 1106 38-39 0.9071451484029893 1106 40-41 6.907043394127623 1106 42-43 8.462832835346603 1106 44-45 -3.5183096038166255 1106 46-47 -4.7865711078383075 1106 48-49 4.93685179006394 1106 50-51 -10.44362727674204 1106 52-53 0.19987068857539114 1106 54-55 5.166885220727178 1106 56-57 6.340021933921644 1106 58-59 6.522834545842535 1106 60-61 6.64350118772801 1106 62-63 6.525480634373356 1106 64-65 6.507099912398282 1106 66-67 6.491826742974151 1106 68-69 6.307515937753067 1106 70-71 6.228981248476774 1106 72-73 6.078312287371411 1106 74-75 6.048180362962324 1106 76-77 5.7906536271559155 1106 78-79 5.880502858744414 1106 80-81 5.325845340277379 1106 82-83 5.286049610608762 1106 84-85 5.206895645849384 1106 86-87 4.848822353601118 1106 88-89 5.05101871990256 1106 90-91 4.581369489352937 1106 92-93 4.550603508955531 1106 94-95 4.1881794634778124 1106 96-97 4.050433896458598 1106 98-99 3.853602616879929 1106 100-101 3.570744941660294 1107 1 6.145728946388502 1107 2 7.538548136522746 1107 3 7.112451942329976 1107 4 8.178095393942641 1107 5 8.685169909421536 1107 6 7.405711965259979 1107 7 7.498469563727024 1107 8 6.737043949088605 1107 9 6.833482981437211 1107 10-11 6.116951392436189 1107 12-13 5.931922210043275 1107 14-15 6.359307071436703 1107 16-17 7.079732794086983 1107 18-19 7.685939593745815 1107 20-21 8.146091417082456 1107 22-23 7.92322755250321 1107 24-25 7.9100046547299705 1107 26-27 7.927424125515582 1107 28-29 8.051704412126881 1107 30-31 7.815551120020164 1107 32-33 7.591770334144922 1107 34-35 9.957125456383285 1107 36-37 -0.12072990890850122 1107 38-39 1.2461509288805885 1107 40-41 7.913338288981752 1107 42-43 9.00597742647114 1107 44-45 -3.3460836108979937 1107 46-47 -4.251197780613502 1107 48-49 5.808054321709509 1107 50-51 -10.37250605073781 1107 52-53 0.9520353982506222 1107 54-55 5.94356831820345 1107 56-57 7.196924287967416 1107 58-59 7.483411490673475 1107 60-61 7.540435187343398 1107 62-63 7.098610887424794 1107 64-65 7.212361447901273 1107 66-67 7.056079126996952 1107 68-69 7.027084579510515 1107 70-71 7.250484823096972 1107 72-73 6.803912393705051 1107 74-75 6.870850355383226 1107 76-77 6.781039058316196 1107 78-79 6.732483322766317 1107 80-81 6.785057764344984 1107 82-83 6.439366597973157 1107 84-85 6.406028008605459 1107 86-87 6.267943178026179 1107 88-89 6.179238988904041 1107 90-91 6.03799370740477 1107 92-93 5.696016598173982 1107 94-95 5.353888278988951 1107 96-97 5.2094131500869505 1107 98-99 5.199943863019678 1107 100-101 4.77115415648818 1108 1 5.878351163760829 1108 2 6.321116701923589 1108 3 6.634774345210811 1108 4 8.015941403690931 1108 5 8.335963776728388 1108 6 7.412013798520565 1108 7 7.53491425672863 1108 8 6.457544458327657 1108 9 4.945356435532947 1108 10-11 5.677689516254031 1108 12-13 5.325355981685938 1108 14-15 5.912217918228492 1108 16-17 6.062599400736186 1108 18-19 6.912266798023424 1108 20-21 7.607028917082456 1108 22-23 7.615173163953809 1108 24-25 7.223371952489327 1108 26-27 7.461090737620925 1108 28-29 7.360682933151175 1108 30-31 7.1535755907752865 1108 32-33 7.0930059208610565 1108 34-35 9.652899454273577 1108 36-37 -3.902723306987941 1108 38-39 -2.540518829594607 1108 40-41 5.729668948810069 1108 42-43 8.110910225103467 1108 44-45 -3.393594023017883 1108 46-47 -4.536234109363685 1108 48-49 5.314168600408767 1108 50-51 -10.570323824999413 1108 52-53 0.3985039163649837 1108 54-55 5.398482475049079 1108 56-57 6.542544150109126 1108 58-59 7.134698683311335 1108 60-61 7.047208748743078 1108 62-63 7.114670104651619 1108 64-65 6.910706648323213 1108 66-67 6.695561749214328 1108 68-69 6.653060999923724 1108 70-71 6.790804507005017 1108 72-73 6.711600312008557 1108 74-75 6.479101164709572 1108 76-77 6.495526454649674 1108 78-79 6.625780691091649 1108 80-81 6.543361316287363 1108 82-83 6.498017751035865 1108 84-85 6.532561409231098 1108 86-87 6.202611672497298 1108 88-89 5.865570467152258 1108 90-91 5.937418994761092 1108 92-93 5.893948041866691 1108 94-95 5.6857348161634 1108 96-97 5.672073878662539 1108 98-99 5.4677751866774695 1108 100-101 4.886420643102468 1109 1 4.801005712768028 1109 2 6.305145978771943 1109 3 6.086892124859844 1109 4 7.709285549716945 1109 5 7.709246500922145 1109 6 4.998803394143696 1109 7 4.808569105808406 1109 8 3.584044821206124 1109 9 3.0255822343307983 1109 10-11 2.5451001806937974 1109 12-13 3.255581984021383 1109 14-15 4.01704734208915 1109 16-17 2.951476158137975 1109 18-19 4.005694336311755 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2313 88-89 -12.07526736358611 2313 90-91 -11.495443504044907 2313 92-93 -10.868057790829843 2313 94-95 -10.418791045905564 2313 96-97 -9.603716400972576 2313 98-99 -8.704922629494083 2313 100-101 -7.528076473384303 2314 1 -21.34794193968745 2314 2 -20.14763329807641 2314 3 -20.62330755134091 2314 4 -22.572959458378037 2314 5 -22.561665533616438 2314 6 -23.361693098031157 2314 7 -23.492564191547235 2314 8 -24.344179679603375 2314 9 -25.607445288604982 2314 10-11 -25.926297552711493 2314 12-13 -25.910904794176133 2314 14-15 -26.17075621970254 2314 16-17 -25.300277754436227 2314 18-19 -24.628142684735195 2314 20-21 -24.103908582917544 2314 22-23 -24.05804881880481 2314 24-25 -23.767737961303784 2314 26-27 -23.45970667617218 2314 28-29 -23.459898963400548 2314 30-31 -23.431284323017817 2314 32-33 -23.20306088948377 2314 34-35 -20.214288045726423 2314 36-37 -18.486236238022425 2314 38-39 -17.552372277870468 2314 40-41 -19.014404327052002 2314 42-43 -18.962904430068946 2314 44-45 -16.904100488535125 2314 46-47 -17.800512126605064 2314 48-49 -17.26314820993606 2314 50-51 -15.093761324999413 2314 52-53 -18.151603842255707 2314 54-55 -19.92694855943368 2314 56-57 -20.202337315408112 2314 58-59 -19.82381424772315 2314 60-61 -19.498858061601748 2314 62-63 -19.034036791900103 2314 64-65 -18.503355851676787 2314 66-67 -18.07841454388912 2314 68-69 -17.618221327662482 2314 70-71 -17.020348510236364 2314 72-73 -16.57530701557765 2314 74-75 -16.039487197359392 2314 76-77 -15.458676131557223 2314 78-79 -14.979014567529042 2314 80-81 -14.475227045781601 2314 82-83 -13.982046904136547 2314 84-85 -13.452621780424076 2314 86-87 -12.888174965433734 2314 88-89 -12.316541601813258 2314 90-91 -11.732839625928563 2314 92-93 -11.088002389167793 2314 94-95 -10.418791045905564 2314 96-97 -9.645544655820222 2314 98-99 -8.80242955470184 2314 100-101 -7.639710822414774 2315 1 -21.34794193968745 2315 2 -20.14763329807641 2315 3 -20.62330755134091 2315 4 -17.036798501336165 2315 5 -22.561665533616438 2315 6 -23.361693098031157 2315 7 -23.492564191547235 2315 8 -24.344179679603375 2315 9 -20.529141862830105 2315 10-11 -18.943426427099745 2315 12-13 -15.163759606574175 2315 14-15 -15.103600156624779 2315 16-17 -20.82393191430572 2315 18-19 -24.628142684735195 2315 20-21 -24.103908582917544 2315 22-23 -24.05804881880481 2315 24-25 -23.767737961303784 2315 26-27 -23.45970667617218 2315 28-29 -23.459898963400548 2315 30-31 -23.431284323017817 2315 32-33 -23.20306088948377 2315 34-35 -20.214288045726423 2315 36-37 -18.486236238022425 2315 38-39 -17.552372277870468 2315 40-41 -19.014404327052002 2315 42-43 -18.962904430068946 2315 44-45 -16.904100488535125 2315 46-47 -17.800512126605064 2315 48-49 -17.26314820993606 2315 50-51 -15.093761324999413 2315 52-53 -18.151603842255707 2315 54-55 -19.92694855943368 2315 56-57 -20.202337315408112 2315 58-59 -19.82381424772315 2315 60-61 -19.498858061601748 2315 62-63 -19.034036791900103 2315 64-65 -18.503355851676787 2315 66-67 -18.07841454388912 2315 68-69 -17.618221327662482 2315 70-71 -17.020348510236364 2315 72-73 -16.57530701557765 2315 74-75 -16.039487197359392 2315 76-77 -15.458676131557223 2315 78-79 -14.979014567529042 2315 80-81 -14.475227045781601 2315 82-83 -13.982046904136547 2315 84-85 -13.452621780424076 2315 86-87 -12.888174965433734 2315 88-89 -12.316541601813258 2315 90-91 -11.732839625928563 2315 92-93 -11.088002389167793 2315 94-95 -10.418791045905564 2315 96-97 -9.645544655820222 2315 98-99 -8.80242955470184 2315 100-101 -7.639710822414774 2316 1 -21.34794193968745 2316 2 -20.14763329807641 2316 3 -20.62330755134091 2316 4 -22.572959458378037 2316 5 -22.561665533616438 2316 6 -23.361693098031157 2316 7 -23.492564191547235 2316 8 -24.344179679603375 2316 9 -25.607445288604982 2316 10-11 -25.926297552711493 2316 12-13 -25.910904794176133 2316 14-15 -26.17075621970254 2316 16-17 -25.300277754436227 2316 18-19 -24.628142684735195 2316 20-21 -24.103908582917544 2316 22-23 -24.05804881880481 2316 24-25 -23.767737961303784 2316 26-27 -23.45970667617218 2316 28-29 -23.459898963400548 2316 30-31 -23.431284323017817 2316 32-33 -23.20306088948377 2316 34-35 -20.214288045726423 2316 36-37 -18.486236238022425 2316 38-39 -17.552372277870468 2316 40-41 -19.014404327052002 2316 42-43 -18.962904430068946 2316 44-45 -16.904100488535125 2316 46-47 -17.800512126605064 2316 48-49 -17.26314820993606 2316 50-51 -15.093761324999413 2316 52-53 -18.151603842255707 2316 54-55 -19.92694855943368 2316 56-57 -20.202337315408112 2316 58-59 -19.82381424772315 2316 60-61 -19.498858061601748 2316 62-63 -19.034036791900103 2316 64-65 -18.503355851676787 2316 66-67 -18.07841454388912 2316 68-69 -17.618221327662482 2316 70-71 -17.020348510236364 2316 72-73 -16.57530701557765 2316 74-75 -16.039487197359392 2316 76-77 -15.458676131557223 2316 78-79 -14.979014567529042 2316 80-81 -14.475227045781601 2316 82-83 -13.982046904136547 2316 84-85 -13.452621780424076 2316 86-87 -12.888174965433734 2316 88-89 -12.316541601813258 2316 90-91 -11.732839625928563 2316 92-93 -11.088002389167793 2316 94-95 -10.418791045905564 2316 96-97 -9.645544655820222 2316 98-99 -8.80242955470184 2316 100-101 -7.639710822414774 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 272091.0 3 15236.0 4 34378.0 5 18214.0 6 14750.0 7 18635.0 8 24471.0 9 30001.0 10 32753.0 11 31356.0 12 28457.0 13 26272.0 14 25134.0 15 27123.0 16 29619.0 17 32113.0 18 33670.0 19 35074.0 20 37332.0 21 39731.0 22 42703.0 23 45520.0 24 48918.0 25 52635.0 26 56470.0 27 61008.0 28 65518.0 29 70751.0 30 75294.0 31 75863.0 32 74675.0 33 73906.0 34 70944.0 35 61963.0 36 46072.0 37 21466.0 38 2924.0 39 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.12628902104483 19.081716425381952 13.320718447768368 24.471276105804847 2 15.816917434363633 20.3430801258522 39.451455193291174 24.38854724649299 3 18.837136880861372 25.301215610299206 29.71210897776847 26.149538531070952 4 12.989145548527212 16.389677309237836 37.611255552661824 33.00992158957313 5 13.66844173491498 37.44507694002718 34.84299827389915 14.043483051158692 6 31.78292622019968 36.299422246143145 17.78210834228667 14.135543191370504 7 27.49814415331106 32.033793189546046 22.428628767570135 18.03943388957276 8 25.91294271679815 34.991471523843074 20.69989950087815 18.395686258480627 9 26.646454686018078 15.591309279781978 20.19007420342517 37.57216183077477 10-11 25.2421291990421 25.704417461863503 28.626628536276357 20.426824802818036 12-13 25.555054600190285 23.68466674240165 28.345947941665127 22.414330715742935 14-15 22.75200790090821 24.97530966184409 25.93604431807814 26.336638119169564 16-17 22.714623213300115 27.88644558270531 26.523949902766386 22.874981301228186 18-19 22.66673659294455 27.114949781686377 28.148277558775654 22.07003606659342 20-21 23.384921570167858 26.43600016920781 27.98643383135488 22.19264442926946 22-23 23.12902660404835 26.28036385020746 27.778911692774923 22.811697852969264 24-25 22.923854003049644 26.44070164828081 27.77099028473496 22.864454063934584 26-27 22.869147442517775 26.633143909640733 27.64240147743265 22.855307170408842 28-29 22.874390693046383 26.773627237131787 27.617695490661205 22.734286579160628 30-31 23.19606765816419 26.540061792239904 27.582362882529498 22.681507667066406 32-33 22.95794582466975 26.629179878329573 27.50979829968624 22.90307599731444 34-35 23.244580967223698 26.63549454105127 27.501564846828696 22.618359644896337 36-37 23.7195449422621 26.6606894020396 27.04563375080361 22.57413190489469 38-39 23.493073706559798 26.71473919841952 27.063213760110234 22.728973334910453 40-41 23.46935716626116 26.556402194599944 27.30910535892306 22.665135280215836 42-43 23.49295458685987 26.665140923938242 26.995161166155125 22.846743323046763 44-45 23.724907376252325 26.724862289363184 26.82837582493171 22.721854509452783 46-47 23.155301439250895 26.759742324496838 27.023378176617374 23.061578059634893 48-49 23.545685710429108 26.897078400846414 26.616382799672117 22.940853089052364 50-51 23.874481682899148 26.98208407758121 26.495417509926224 22.64801672959342 52-53 22.947700878668428 26.868897734203923 27.01559956217619 23.167801824951457 54-55 22.74108847242552 26.85516623766343 27.186836365159873 23.216908924751174 56-57 22.774195354627338 27.012020360780102 26.950622894548715 23.263161390043845 58-59 22.81627252584228 26.910589645037753 27.0694822453 23.203655583819963 60-61 22.828316730177704 27.003002012791182 27.105326258305258 23.063354998725856 62-63 22.793777637665908 26.938683542879794 26.992348278575246 23.275190540879052 64-65 22.59323256999477 26.822545300469393 27.047740107383795 23.536482022152043 66-67 22.638452031244622 26.953292187350826 26.903737755086798 23.504518026317754 68-69 22.72627492321838 26.868703266398207 26.963082385215344 23.44193942516807 70-71 22.53816957463221 26.772991094170216 26.955280023416815 23.733559307780755 72-73 22.62561553418011 26.88505586569755 26.842636790279258 23.646691809843087 74-75 22.642517287970865 26.985690098257642 26.738374731472398 23.6334178822991 76-77 22.627715630421815 26.823056106279214 26.782181959096913 23.76704630420206 78-79 22.57230745769754 26.766060848081402 26.80654352566321 23.85508816855785 80-81 22.68156684424097 26.89212239997597 26.648290154235443 23.77802060154762 82-83 22.730844781232054 26.820069780088534 26.73716463120232 23.711920807477092 84-85 22.63759965885641 26.880160621774813 26.643879340760314 23.83836037860847 86-87 22.489099551970217 26.912865372753853 26.62867994390651 23.969355131369426 88-89 22.55046844936914 26.723650550363836 26.60076270537946 24.125118294887564 90-91 22.54867431091289 26.759947062841533 26.59210136411122 24.09927726213436 92-93 22.58514339949417 26.550619509317926 26.702843908824615 24.161393182363287 94-95 22.438396534297887 26.483793889707503 26.791092142876654 24.28671743311796 96-97 22.452375430824222 26.360125068971858 26.66013066668799 24.527368833515926 98-99 22.33049757066898 26.307844673510555 26.617792702620385 24.743865053200082 100-101 22.582581565107766 26.44524731078311 26.457649368885473 24.514521755223647 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21039.0 1 22897.0 2 16870.5 3 9627.5 4 12640.5 5 16202.5 6 17377.5 7 16744.0 8 15296.5 9 13889.5 10 12439.0 11 10637.0 12 9068.5 13 7720.5 14 6483.0 15 5564.0 16 4897.5 17 4499.0 18 4283.5 19 4212.0 20 4377.5 21 4825.0 22 5471.5 23 6127.0 24 6819.0 25 7883.0 26 9135.5 27 10427.0 28 11998.0 29 14028.5 30 16520.0 31 19560.0 32 23471.5 33 27309.0 34 31278.0 35 35856.0 36 40827.0 37 45180.5 38 48172.5 39 50557.5 40 52130.5 41 53465.5 42 54731.0 43 55970.5 44 57639.5 45 58851.5 46 59669.5 47 60112.5 48 59789.5 49 59029.5 50 57403.0 51 55420.5 52 53405.0 53 50713.5 54 47456.5 55 43518.5 56 38916.0 57 34372.5 58 29859.0 59 25549.0 60 21638.0 61 17885.5 62 14562.0 63 11553.5 64 8932.5 65 6829.5 66 5194.0 67 3943.5 68 3031.0 69 2367.0 70 1829.5 71 1421.5 72 1081.0 73 843.0 74 670.5 75 500.0 76 358.5 77 256.5 78 168.5 79 107.0 80 67.5 81 41.0 82 30.0 83 19.0 84 12.0 85 7.5 86 6.0 87 2.5 88 0.5 89 1.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.536296532919579 2 21.44187877197586 3 17.204659281484947 4 22.46369205845778 5 22.338653554356384 6 16.16618940595302 7 15.012264269334763 8 12.079050346251698 9 3.0465585889815525 10-11 6.5298392525070446 12-13 3.591206233671409 14-15 7.4708224476059 16-17 11.531607589015778 18-19 14.617041059632871 20-21 15.046090835453436 22-23 12.942084127183326 24-25 15.491597549427857 26-27 13.03569187591982 28-29 13.033181978939682 30-31 13.87992995105701 32-33 12.361042976281473 34-35 19.256956407652904 36-37 24.00314307553649 38-39 23.080299590430446 40-41 18.175647154119083 42-43 18.12222627862138 44-45 23.45649892188516 46-47 12.813366342281496 48-49 21.607674580447902 50-51 21.262906004358094 52-53 11.247932187147045 54-55 12.86253750584692 56-57 12.387824717921806 58-59 13.408183405017512 60-61 12.811056096197508 62-63 12.784673656349469 64-65 13.838088827535852 66-67 12.169520723763021 68-69 13.207591297502653 70-71 11.817193935176206 72-73 11.179166714202594 74-75 11.257686559501671 76-77 11.310936305659817 78-79 11.721475591251867 80-81 11.697802699280116 82-83 10.20912005293601 84-85 10.107126966561326 86-87 10.245827296270521 88-89 11.664945866085588 90-91 9.139903939397396 92-93 11.170153902319372 94-95 11.25132625238155 96-97 10.833770663867751 98-99 10.666548777566085 100-101 10.769939420213797 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1753060.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 97.41734141700609 #Duplication Level Percentage of deduplicated Percentage of total 1 98.7448294166201 96.19458760442907 2 0.9246928057071638 1.8016222951884813 3 0.1756532220976109 0.5133500972424047 4 0.06500821847364717 0.2533171125583443 5 0.03361144507124264 0.16371688100120954 6 0.013325937889722896 0.07789064646629718 7 0.010362550475442059 0.07066444823319686 8 0.0060580763479588925 0.04721293535355206 9 0.004398450010464204 0.038563677573752286 >10 0.019857122517272195 0.33735247725768314 >50 0.0012033993471738424 0.07999339807500624 >100 7.642933686040591E-4 0.1054343000631023 >500 1.1753103683414234E-4 0.07510912109392331 >1k 1.1753103683414208E-4 0.2411850054639469 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3204 0.18276613464456437 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.7043113184945184E-5 2 0.0 0.0 0.0 0.0 5.7043113184945184E-5 3 0.0 0.0 0.0 1.1408622636989037E-4 5.7043113184945184E-5 4 0.0 0.0 0.0 1.7112933955483554E-4 5.7043113184945184E-5 5 0.0 0.0 0.0 1.7112933955483554E-4 5.7043113184945184E-5 6 0.0 0.0 0.0 1.7112933955483554E-4 5.7043113184945184E-5 7 0.0 0.0 0.0 1.7112933955483554E-4 5.7043113184945184E-5 8 0.0 0.0 0.0 1.7112933955483554E-4 5.7043113184945184E-5 9 0.0 0.0 0.0 1.7112933955483554E-4 5.7043113184945184E-5 10-11 0.0 0.0 2.8521556592472592E-5 1.7112933955483554E-4 5.7043113184945184E-5 12-13 0.0 0.0 5.7043113184945184E-5 1.7112933955483554E-4 5.7043113184945184E-5 14-15 0.0 0.0 5.7043113184945184E-5 1.7112933955483554E-4 1.1408622636989037E-4 16-17 0.0 0.0 5.7043113184945184E-5 1.7112933955483554E-4 1.7112933955483554E-4 18-19 0.0 0.0 5.7043113184945184E-5 2.2817245273978073E-4 2.2817245273978073E-4 20-21 0.0 0.0 5.7043113184945184E-5 2.566940093322533E-4 3.137371225171985E-4 22-23 5.7043113184945184E-5 0.0 5.7043113184945184E-5 3.137371225171985E-4 3.707802357021437E-4 24-25 5.7043113184945184E-5 0.0 5.7043113184945184E-5 4.8486646207203404E-4 3.9930179229461627E-4 26-27 5.7043113184945184E-5 0.0 5.7043113184945184E-5 6.845173582193421E-4 4.2782334888708884E-4 28-29 5.7043113184945184E-5 0.0 5.7043113184945184E-5 0.0011979053768838488 4.5634490547956147E-4 30-31 8.556466977741777E-5 0.0 5.7043113184945184E-5 0.004278233488870889 4.5634490547956147E-4 32-33 1.1408622636989037E-4 0.0 5.7043113184945184E-5 0.013234002258907283 4.5634490547956147E-4 34-35 1.1408622636989037E-4 0.0 5.7043113184945184E-5 0.026610612300776926 6.27474245034397E-4 36-37 1.1408622636989037E-4 0.0 5.7043113184945184E-5 0.04845812465061093 6.27474245034397E-4 38-39 1.1408622636989037E-4 0.0 5.7043113184945184E-5 0.0786624530820394 6.27474245034397E-4 40-41 1.1408622636989037E-4 0.0 5.7043113184945184E-5 0.11402918325670541 6.27474245034397E-4 42-43 1.1408622636989037E-4 0.0 5.7043113184945184E-5 0.1501659954593682 6.27474245034397E-4 44-45 1.1408622636989037E-4 0.0 5.7043113184945184E-5 0.196142744686434 6.27474245034397E-4 46-47 1.7112933955483554E-4 0.0 5.7043113184945184E-5 0.24782380523199432 6.27474245034397E-4 48-49 1.7112933955483554E-4 0.0 5.7043113184945184E-5 0.3030130172384288 6.27474245034397E-4 50-51 1.7112933955483554E-4 0.0 5.7043113184945184E-5 0.3724344859845071 6.845173582193421E-4 52-53 1.7112933955483554E-4 0.0 5.7043113184945184E-5 0.46102244076072696 7.415604714042874E-4 54-55 1.7112933955483554E-4 0.0 5.7043113184945184E-5 0.5535178487901156 7.415604714042874E-4 56-57 1.7112933955483554E-4 0.0 5.7043113184945184E-5 0.6575644872394556 7.986035845892325E-4 58-59 1.7112933955483554E-4 0.0 5.7043113184945184E-5 0.7687700363935062 7.986035845892325E-4 60-61 1.7112933955483554E-4 0.0 5.7043113184945184E-5 0.8944074931833479 7.986035845892325E-4 62-63 1.9965089614730814E-4 0.0 5.7043113184945184E-5 1.0217277218121457 8.556466977741777E-4 64-65 2.2817245273978073E-4 0.0 5.7043113184945184E-5 1.154039222844626 8.556466977741777E-4 66-67 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 1.2986720363250546 8.841682543666503E-4 68-69 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 1.4532303514996634 9.126898109591229E-4 70-71 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 1.6193684186508164 9.126898109591229E-4 72-73 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 1.7923516593841624 9.412113675515955E-4 74-75 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 1.9744047551139152 9.697329241440681E-4 76-77 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 2.1701481980080546 9.697329241440681E-4 78-79 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 2.3938712879194095 9.697329241440681E-4 80-81 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 2.633880186645066 0.001055297593921486 82-83 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 2.8791085302271457 0.0011408622636989037 84-85 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 3.129898577344757 0.0011408622636989037 86-87 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 3.3936944542685366 0.0011408622636989037 88-89 2.2817245273978073E-4 1.1408622636989037E-4 5.7043113184945184E-5 3.6706102472248525 0.0011408622636989037 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1295 0.0 50.5615 1 GTATCAA 2440 0.0 47.889965 1 TCAACGC 3355 0.0 34.199757 4 ATCAACG 3295 0.0 34.071896 3 CAACGCA 3515 0.0 32.32101 5 TATCAAC 3500 0.0 32.082397 2 AACGCAG 3815 0.0 29.892612 6 GTACATG 4000 0.0 28.709208 1 TACATGG 4010 0.0 27.374245 2 ACATGGG 4165 0.0 25.38348 3 ACGCAGA 4570 0.0 24.672323 7 CGCAGAG 4670 0.0 24.671574 8 CATGGGG 2995 0.0 23.019955 4 GCAGAGT 5090 0.0 21.452427 9 GTGGTAT 765 0.0 19.751778 1 AGAGTAC 4760 0.0 19.471085 10-11 AGTACTT 3270 0.0 18.650215 12-13 GGGTACG 165 9.881776E-4 18.315285 1 GTATAAG 550 0.0 18.315285 1 CAGAGTA 4940 0.0 17.56074 9 >>END_MODULE