FastQCFastQC Report
Sat 18 Jun 2016
SRR1930929_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930929_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1753060
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27720.15812350974866804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.13895702371852647No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.053.448971
GTATCAA34350.042.574271
ATCAACG44450.032.2521933
TCAACGC44900.032.2461284
CAACGCA45250.031.5689755
TATCAAC47100.030.9423962
AACGCAG48650.029.557826
ACGCAGA55450.025.8474677
GTACATG52100.025.5178181
CGCAGAG57550.024.8218278
ACATGGG53100.024.4951523
TACATGG54300.024.3042052
GTGGTAT8700.024.0135651
GCAGAGT65850.021.188699
CATGGGG38150.020.1578664
GTGTACG2151.0835247E-619.8759211
GAGTACA40600.019.2966161
AGAGTAC61700.019.22947510-11
GAGTACT40600.019.05347312-13
CAGAGTA63450.018.43732510-11
Produced by FastQC (version 0.11.5)