##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930929_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1753060 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.31747915074213 31.0 31.0 34.0 30.0 34.0 2 31.45373176046456 31.0 31.0 34.0 30.0 34.0 3 31.536265729638462 31.0 31.0 34.0 30.0 34.0 4 35.39659110355607 37.0 35.0 37.0 33.0 37.0 5 35.19650896147308 37.0 35.0 37.0 32.0 37.0 6 35.23976133161443 37.0 35.0 37.0 32.0 37.0 7 35.11877288854917 37.0 35.0 37.0 32.0 37.0 8 35.09046923664906 37.0 35.0 37.0 32.0 37.0 9 36.6227835898372 39.0 35.0 39.0 32.0 39.0 10-11 36.54430167820839 38.0 35.0 39.0 32.0 39.0 12-13 36.441473765872246 38.0 35.0 39.0 32.0 39.0 14-15 37.48366484889279 39.0 36.0 41.0 32.0 41.0 16-17 37.44836001049593 39.0 36.0 41.0 32.0 41.0 18-19 37.425920390631234 39.0 36.0 41.0 32.0 41.0 20-21 37.3889521750539 39.0 36.0 40.5 32.0 41.0 22-23 37.26046370346708 39.0 36.0 40.0 31.0 41.0 24-25 37.143591491449236 39.0 36.0 40.0 31.0 41.0 26-27 36.88849326320833 39.0 36.0 40.0 31.0 41.0 28-29 36.70014203735183 38.5 35.5 40.0 30.0 41.0 30-31 36.46170353553215 38.0 35.0 40.0 30.0 41.0 32-33 36.229994980206044 38.0 35.0 40.0 30.0 41.0 34-35 36.42448176331671 38.0 35.0 40.0 30.0 41.0 36-37 36.4885705566267 38.0 35.0 40.0 30.0 41.0 38-39 36.45245028692686 38.0 35.0 40.0 30.0 41.0 40-41 36.36169783122084 38.0 35.0 40.0 30.0 41.0 42-43 36.21739358607235 38.0 35.0 40.0 30.0 41.0 44-45 36.03474496024096 38.0 35.0 40.0 29.5 41.0 46-47 35.809076129738855 38.0 35.0 40.0 28.5 41.0 48-49 35.614075673393955 38.0 34.0 40.0 28.0 41.0 50-51 35.34156389399108 38.0 34.0 40.0 27.5 41.0 52-53 35.11688390585605 38.0 34.0 40.0 27.0 41.0 54-55 34.94094925444651 38.0 33.5 40.0 26.5 41.0 56-57 34.68043791997992 37.5 33.0 40.0 26.0 41.0 58-59 34.42403197836925 37.0 33.0 40.0 26.0 41.0 60-61 34.10447018356474 37.0 33.0 40.0 26.0 41.0 62-63 33.779988135032454 36.0 32.0 39.0 25.0 41.0 64-65 33.45330992664256 36.0 32.0 39.0 24.5 40.5 66-67 33.09939591343137 35.5 31.5 39.0 23.5 40.0 68-69 32.723902490502326 35.0 31.0 38.5 22.5 40.0 70-71 32.30907356279876 35.0 31.0 38.0 22.0 40.0 72-73 31.88449368532737 35.0 31.0 37.0 21.5 39.0 74-75 31.360435752341623 34.0 30.0 36.5 20.0 39.0 76-77 29.99459773196582 33.0 28.5 35.0 19.5 37.5 78-79 30.74646218612027 34.0 30.0 36.0 20.0 37.5 80-81 30.741242456048283 34.0 30.0 35.0 20.0 37.0 82-83 30.4843285455147 34.0 30.0 35.0 19.5 37.0 84-85 30.184654261690987 34.0 30.0 35.0 18.0 36.0 86-87 29.866851961712662 34.0 29.5 35.0 17.5 36.0 88-89 29.542717020524112 34.0 29.0 35.0 13.5 35.5 90-91 29.22312413722291 34.0 29.0 35.0 8.5 35.0 92-93 28.889557402484797 34.0 29.0 35.0 4.5 35.0 94-95 28.49230516924692 33.0 29.0 35.0 2.0 35.0 96-97 28.048480371464752 33.0 28.5 35.0 2.0 35.0 98-99 27.57946390882229 33.0 27.0 35.0 2.0 35.0 100-101 26.388447913933348 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.36661100224851495 1101 2 0.4712842395740644 1101 3 0.41655519080538284 1101 4 0.29543991406386994 1101 5 0.3613910560481557 1101 6 0.3892743233694276 1101 7 0.40053508293528495 1101 8 0.37801644070989937 1101 9 0.48128089470782953 1101 10-11 0.4213429745154045 1101 12-13 0.4203806853915353 1101 14-15 0.6063325781084004 1101 16-17 0.6183024380222122 1101 18-19 0.613610708892196 1101 20-21 0.5597125883923582 1101 22-23 0.54890144384796 1101 24-25 0.5108505744248006 1101 26-27 0.5219892109592266 1101 28-29 0.5490326613220518 1101 30-31 0.39975407317749756 1101 32-33 0.3748363369180865 1101 34-35 0.3149895363152737 1101 36-37 0.4001681115395428 1101 38-39 0.5059492912180943 1101 40-41 0.39617776949995687 1101 42-43 0.37124610322016593 1101 44-45 0.49520252796971675 1101 46-47 0.5071351795273245 1101 48-49 0.4741352276261068 1101 50-51 0.46498876962909463 1101 52-53 0.5069554556026006 1101 54-55 0.4020374718634159 1101 56-57 0.4214041864802027 1101 58-59 0.32049737376448206 1101 60-61 0.34929246647488554 1101 62-63 0.38014569720205316 1101 64-65 0.45520212935367255 1101 66-67 0.4821286184563647 1101 68-69 0.33374347033060303 1101 70-71 0.28735343397658397 1101 72-73 0.29072936959856577 1101 74-75 0.24177420160530616 1101 76-77 0.3055602435167941 1101 78-79 0.209905898943191 1101 80-81 0.07154081962987746 1101 82-83 0.3775890172249241 1101 84-85 0.42882015606819834 1101 86-87 0.4738195312582043 1101 88-89 0.4894218961489649 1101 90-91 0.4588673260464837 1101 92-93 0.437589492788355 1101 94-95 0.2829676196417452 1101 96-97 0.38518809118575703 1101 98-99 0.4504449013889946 1101 100-101 0.478474745520991 1102 1 0.3492368258324525 1102 2 0.429107973822628 1102 3 0.5049868114181564 1102 4 0.2875197296812786 1102 5 0.3712778210856058 1102 6 0.36932856091232935 1102 7 0.40827364044211123 1102 8 0.44177123190162604 1102 9 0.5485592472894325 1102 10-11 0.49550717773709607 1102 12-13 0.5108048780913848 1102 14-15 0.6188942686162022 1102 16-17 0.6482652358276653 1102 18-19 0.5986568671706181 1102 20-21 0.5947960114952977 1102 22-23 0.798952487263044 1102 24-25 0.749148480079036 1102 26-27 0.6577308749119268 1102 28-29 0.7065708029659561 1102 30-31 0.6409890106728042 1102 32-33 0.6548915139103428 1102 34-35 0.8446614179697036 1102 36-37 0.6955591729920556 1102 38-39 0.7248212630596669 1102 40-41 0.6268739641037584 1102 42-43 0.5318224240859593 1102 44-45 0.6534865924034108 1102 46-47 0.5840150176915131 1102 48-49 0.7775015878235152 1102 50-51 0.6779331291751518 1102 52-53 0.5352811063278509 1102 54-55 0.5735048291297389 1102 56-57 0.5713321620294707 1102 58-59 0.7590758616267976 1102 60-61 0.7181578621709619 1102 62-63 0.535207137190028 1102 64-65 0.5334413507380518 1102 66-67 0.6083877937450808 1102 68-69 0.5634147256854618 1102 70-71 0.7619347527129534 1102 72-73 0.8106046733538577 1102 74-75 0.9624054489435316 1102 76-77 1.0031752947480967 1102 78-79 0.9913984334523 1102 80-81 0.8009393215213017 1102 82-83 0.9956583861654771 1102 84-85 1.118681931752743 1102 86-87 0.9528710631869579 1102 88-89 0.9463684978474909 1102 90-91 0.9337038618582945 1102 92-93 1.1328797200098393 1102 94-95 1.0949477909973062 1102 96-97 1.1747642742656446 1102 98-99 1.200048140610381 1102 100-101 1.3975656090628412 1103 1 0.5244365334500571 1103 2 0.5471186678929811 1103 3 0.4719190382847813 1103 4 0.27984530607692193 1103 5 0.3870833622233789 1103 6 0.4197304331424476 1103 7 0.4976416092137086 1103 8 0.4996251696966567 1103 9 0.6460224000438757 1103 10-11 0.5730643719717534 1103 12-13 0.6097773935320561 1103 14-15 0.7731815711736303 1103 16-17 0.6756754710542126 1103 18-19 0.65096106771054 1103 20-21 0.6115246149086815 1103 22-23 0.805287541516563 1103 24-25 0.696929352987901 1103 26-27 0.7772315828335863 1103 28-29 0.6461798643631838 1103 30-31 0.7748107741194659 1103 32-33 0.8121147718241701 1103 34-35 0.7528164912035109 1103 36-37 0.8311054397418545 1103 38-39 0.797669012298762 1103 40-41 0.9049386813497122 1103 42-43 1.0043876189103003 1103 44-45 1.0933330609339151 1103 46-47 0.9494131583606134 1103 48-49 1.0952093037089057 1103 50-51 1.101782257402654 1103 52-53 0.923860533295219 1103 54-55 1.0589396097354538 1103 56-57 1.0917347406456273 1103 58-59 1.157536247190727 1103 60-61 0.9754989022783249 1103 62-63 0.9590442875673375 1103 64-65 0.8472845207528863 1103 66-67 0.9437043314707125 1103 68-69 1.011167343152863 1103 70-71 0.8748117094790615 1103 72-73 0.9678127286116762 1103 74-75 1.0744705224537547 1103 76-77 1.0546974030954317 1103 78-79 1.2380355752301178 1103 80-81 1.1952641239635575 1103 82-83 1.0537431279199545 1103 84-85 1.1427967225549835 1103 86-87 1.1356372401950132 1103 88-89 1.203615097212971 1103 90-91 1.3101142537182682 1103 92-93 1.3602056495985089 1103 94-95 1.333779213505224 1103 96-97 1.1641827649950969 1103 98-99 1.3425690481014492 1103 100-101 1.610648339152739 1104 1 0.3966805775754523 1104 2 0.4904159500848593 1104 3 0.4969913574588105 1104 4 0.2084839940833163 1104 5 0.32194638293545097 1104 6 0.4162442402545352 1104 7 0.42176512561200497 1104 8 0.4677073534332479 1104 9 0.49334314542933555 1104 10-11 0.49801679609158356 1104 12-13 0.5912417597953663 1104 14-15 0.5594859735913573 1104 16-17 0.6857598756378991 1104 18-19 0.7528911921765484 1104 20-21 0.6391227566681223 1104 22-23 0.632030630583273 1104 24-25 0.6474767006159752 1104 26-27 0.8141460099887112 1104 28-29 0.7399405705961115 1104 30-31 0.6969478923947747 1104 32-33 0.837674284536206 1104 34-35 0.6489849545254458 1104 36-37 0.7216254384316514 1104 38-39 0.6646704880436971 1104 40-41 0.606881730756335 1104 42-43 0.6380288491167789 1104 44-45 0.717822813492127 1104 46-47 0.7887667696864042 1104 48-49 0.8484004804008194 1104 50-51 0.6324124485884894 1104 52-53 0.793861008390067 1104 54-55 0.8291441985615151 1104 56-57 0.8622088941579236 1104 58-59 0.837663590315735 1104 60-61 0.8281129715188271 1104 62-63 0.8402224755791821 1104 64-65 0.8715832615744787 1104 66-67 0.9893579955880938 1104 68-69 0.7821521498556478 1104 70-71 0.6998193744409917 1104 72-73 0.7114724993208625 1104 74-75 0.8917978808054023 1104 76-77 0.971801466867916 1104 78-79 0.8977176098872555 1104 80-81 0.7794261437009453 1104 82-83 0.8352459739151534 1104 84-85 0.8284313297621999 1104 86-87 0.7914234970879441 1104 88-89 0.6668971999768729 1104 90-91 0.7469899355605278 1104 92-93 0.7003816766665203 1104 94-95 0.8527579248296249 1104 96-97 0.9098765908571949 1104 98-99 1.1633983866543325 1104 100-101 1.3434004042661094 1105 1 0.1750571798384577 1105 2 0.3140223891353102 1105 3 0.3262703287389712 1105 4 0.2391023990425225 1105 5 0.14232248703596895 1105 6 0.21218578880778693 1105 7 0.24561035093044126 1105 8 0.22637908408461982 1105 9 0.4003740712537436 1105 10-11 0.4335623769584842 1105 12-13 0.3219892237279183 1105 14-15 0.5189719511179973 1105 16-17 0.42081593035685216 1105 18-19 0.36446614918786224 1105 20-21 0.4231327130545992 1105 22-23 0.6071945709399031 1105 24-25 0.5451208953500384 1105 26-27 0.5632265398711738 1105 28-29 0.46280236825348453 1105 30-31 0.43574945986218694 1105 32-33 0.480393548239455 1105 34-35 0.6205283517133751 1105 36-37 0.590431830239595 1105 38-39 0.5780605638576262 1105 40-41 0.5429097456760488 1105 42-43 0.41849704750440964 1105 44-45 0.4588899907896362 1105 46-47 0.6487340659766971 1105 48-49 0.5240284954349121 1105 50-51 0.6715104827912555 1105 52-53 0.4915826156304135 1105 54-55 0.6739404166445908 1105 56-57 0.6983097174414965 1105 58-59 0.5714883944037581 1105 60-61 0.6181632195848721 1105 62-63 0.5881529707589905 1105 64-65 0.5460370773075454 1105 66-67 0.643455639439658 1105 68-69 0.6214253988445222 1105 70-71 0.5029947170563887 1105 72-73 0.5217844056617409 1105 74-75 0.6710038006458525 1105 76-77 0.5394865069225219 1105 78-79 0.5379615036076331 1105 80-81 0.6290322607473975 1105 82-83 0.7199356561463972 1105 84-85 0.4711563128970262 1105 86-87 0.7852392187186332 1105 88-89 0.9374684939782476 1105 90-91 0.6052923053212069 1105 92-93 0.5942636293147743 1105 94-95 0.49761325323795447 1105 96-97 0.2497545267486565 1105 98-99 0.5864288503486677 1105 100-101 0.6990000566390577 1106 1 0.10476275930258083 1106 2 0.1044266192612433 1106 3 0.133418521694189 1106 4 -0.04149909698459453 1106 5 0.11550026630555266 1106 6 0.13512453796685975 1106 7 0.09571054789639533 1106 8 0.17862476733080257 1106 9 0.12379102346628912 1106 10-11 0.12264483556206329 1106 12-13 0.08772686050784273 1106 14-15 0.26936381195347536 1106 16-17 0.0738257672279019 1106 18-19 0.11006669537916736 1106 20-21 0.12622246468324505 1106 22-23 0.2626995479367267 1106 24-25 0.20131646725833008 1106 26-27 0.1149904492525522 1106 28-29 0.06600809983417122 1106 30-31 0.20454885310993376 1106 32-33 0.2641303594478117 1106 34-35 0.13390161147847834 1106 36-37 0.2763745458559015 1106 38-39 0.32567647596425076 1106 40-41 0.35222256446022726 1106 42-43 0.23415591218637388 1106 44-45 0.23605208134255662 1106 46-47 0.12887849815341923 1106 48-49 0.26534064004991365 1106 50-51 0.3619327098063039 1106 52-53 0.28215081741267767 1106 54-55 0.4146426666842231 1106 56-57 0.4098811458088818 1106 58-59 0.25722614544964983 1106 60-61 0.16115964448857056 1106 62-63 0.29773802819906336 1106 64-65 0.29804437318926347 1106 66-67 0.27129911221079794 1106 68-69 0.46766083529050917 1106 70-71 0.37703577567339863 1106 72-73 0.29906004801499364 1106 74-75 0.3405967710038347 1106 76-77 0.22599869552992402 1106 78-79 0.25039763964697315 1106 80-81 0.31800988033029753 1106 82-83 0.4791419933366825 1106 84-85 0.4091203219754611 1106 86-87 0.41539072110179376 1106 88-89 0.23479167097194065 1106 90-91 0.23818361982255354 1106 92-93 0.25954034576807317 1106 94-95 0.2942281240959943 1106 96-97 0.41983069929597505 1106 98-99 0.5446337892092394 1106 100-101 0.6227539506088178 1107 1 0.20863149381915846 1107 2 0.33753795284344434 1107 3 0.36458361032440934 1107 4 0.20631908380521935 1107 5 0.21811562133547113 1107 6 0.32539189919580735 1107 7 0.2577279458896271 1107 8 0.30695108716881236 1107 9 0.17765608155386303 1107 10-11 0.27774588079910956 1107 12-13 0.3170264442229609 1107 14-15 0.28955758804198695 1107 16-17 0.41585357234323084 1107 18-19 0.36440657841215796 1107 20-21 0.4344949955369728 1107 22-23 0.3837496491799257 1107 24-25 0.2728903182328608 1107 26-27 0.4196235981988323 1107 28-29 0.5273419648356921 1107 30-31 0.488957683326781 1107 32-33 0.36819620711921885 1107 34-35 0.38545532693417783 1107 36-37 0.5217398414973502 1107 38-39 0.5482711015906503 1107 40-41 0.42729354782014184 1107 42-43 0.4885404779718243 1107 44-45 0.4824378688336495 1107 46-47 0.4814556748821843 1107 48-49 0.5498989312457141 1107 50-51 0.3747184814604694 1107 52-53 0.27257838610533014 1107 54-55 0.3536703643346968 1107 56-57 0.44263841099819246 1107 58-59 0.44340659024099693 1107 60-61 0.3137030263507583 1107 62-63 0.1161440699859213 1107 64-65 0.17025127695731612 1107 66-67 0.12149565976116605 1107 68-69 0.23814688748448987 1107 70-71 0.25501014813524847 1107 72-73 0.28902410385146027 1107 74-75 0.31052881362778706 1107 76-77 0.3780695148645421 1107 78-79 0.5777525338223803 1107 80-81 0.5079694113310786 1107 82-83 0.40857944313928485 1107 84-85 0.3348933618731955 1107 86-87 0.3077902697494359 1107 88-89 0.3735335969682474 1107 90-91 0.4570414381730252 1107 92-93 0.5770747910797773 1107 94-95 0.6128537618150247 1107 96-97 0.7559012754206798 1107 98-99 0.46947245053659614 1107 100-101 0.7625232971094178 1108 1 0.2625176243298526 1108 2 0.32045116395940454 1108 3 0.35055907591439706 1108 4 0.25011364222221033 1108 5 0.28885210831349895 1108 6 0.22363047769137268 1108 7 0.24979381959834512 1108 8 0.34754926118307594 1108 9 0.3832954493699603 1108 10-11 0.3491700678353382 1108 12-13 0.4108503293531811 1108 14-15 0.5089861945456917 1108 16-17 0.432203783313696 1108 18-19 0.5043927562093415 1108 20-21 0.5807750497345552 1108 22-23 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-0.581323357460434 2316 70-71 -0.5202371256879736 2316 72-73 -0.4443071745747531 2316 74-75 -0.2064837624037743 2316 76-77 -0.5902568317845862 2316 78-79 -0.30705074306950664 2316 80-81 -0.5847459429349122 2316 82-83 -0.7934042271988169 2316 84-85 -0.7872003144926332 2316 86-87 -0.5873868632441521 2316 88-89 -0.8390410646570423 2316 90-91 -0.6324319576294819 2316 92-93 -1.0247438017198256 2316 94-95 -0.7009565839573462 2316 96-97 -0.5446056016684757 2316 98-99 -0.5303679516772242 2316 100-101 -0.31980724155022955 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 13.0 4 43.0 5 51.0 6 111.0 7 310.0 8 723.0 9 1215.0 10 2078.0 11 3115.0 12 4344.0 13 5568.0 14 6587.0 15 8132.0 16 9698.0 17 11092.0 18 10312.0 19 9274.0 20 9132.0 21 9907.0 22 11041.0 23 13081.0 24 15856.0 25 19769.0 26 24848.0 27 32134.0 28 41308.0 29 51013.0 30 63580.0 31 76661.0 32 93028.0 33 113123.0 34 141632.0 35 177481.0 36 231327.0 37 292456.0 38 231406.0 39 31604.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.09504397463364 20.665253620811143 14.116556314061327 24.12314609049389 2 16.238752126357248 21.520674866714053 38.83775109840399 23.40282190852471 3 18.844291766756815 25.78894616837507 30.36201307128396 25.00474899358415 4 13.341200410168385 17.320712533474545 37.461418322110006 31.876668734247065 5 13.359520418111073 37.92828062545529 35.0654610567892 13.646737899644442 6 31.708384196775924 37.351145996143885 17.27470822447606 13.665761582604134 7 27.319886370118535 32.36147079963036 22.34196205492111 17.976680775329996 8 25.787993565536834 35.827923744766295 20.423373986058664 17.960708703638208 9 26.320205811552373 15.548697705725989 19.898349172304428 38.23274731041722 10-11 25.035823075080145 25.944348738776768 28.710711555793868 20.30911663034922 12-13 25.580499241326592 23.448341756699715 28.536587452796823 22.43457154917687 14-15 22.70792862421384 25.047780751309357 25.831867087226644 26.412423537250163 16-17 22.695800486007325 27.924914150114656 26.613350370209805 22.765934993668214 18-19 22.663827821067162 27.000530500952618 28.244897493525606 22.09074418445461 20-21 23.318597195760557 26.295876923778994 28.05448758171426 22.331038298746194 22-23 23.0490882078379 26.197243275110328 28.048864313427075 22.704804203624693 24-25 22.912666495536353 26.413479364536098 28.02926328399076 22.644590855936798 26-27 22.817391105932753 26.579208196129827 27.9069150567092 22.69648564122822 28-29 22.848359837495394 26.73391296808206 27.83507448127786 22.58265271314469 30-31 23.197750752092983 26.526584030328447 27.72705192957095 22.548613288007616 32-33 22.925933491111834 26.667828704580675 27.67980016751604 22.726437636791452 34-35 23.014520551383878 26.645228904637424 27.82461008679698 22.515640457181718 36-37 23.076425044108813 26.51206382472615 27.758376681568304 22.65313444959673 38-39 22.91797332128573 26.50353342226428 27.82631711498646 22.752176141463526 40-41 23.038863493039585 26.392589248100634 27.913706656596425 22.654840602263352 42-43 22.98312520323348 26.490949335676106 27.632137417209098 22.893788043881315 44-45 22.905107927520625 26.607603060620537 27.66127801587358 22.826010995985254 46-47 23.13327957542855 26.5706573686599 27.427313446331425 22.868749609580128 48-49 23.028824431452552 26.678373062055304 27.344844622132165 22.947957884359983 50-51 23.02716067163552 26.720738824220625 27.334130041795763 22.917970462348094 52-53 23.14763819410954 26.624157109388154 27.318958653772068 22.90924604273024 54-55 22.88688167724109 26.651967738429445 27.41845532312206 23.04269526120741 56-57 22.928603641058277 26.807728698571115 27.19920108142677 23.06446657894384 58-59 22.848080935560674 26.802886741221986 27.34196837684422 23.007063946373115 60-61 22.986639666523885 26.878992197601775 27.299062062754214 22.835306073120123 62-63 22.881426823749752 26.897923117222756 27.194273976083515 23.02637608294398 64-65 22.67910136252883 26.78490363053604 27.23418810446835 23.301806902466783 66-67 22.689180723675907 26.8518618982002 27.150409760939 23.308547617184892 68-69 22.805518310998018 26.78176186680128 27.198136350865816 23.214583471334887 70-71 22.74057139202865 26.69732643892902 27.181775202526932 23.380326966515394 72-73 22.789607083121645 26.824126454956986 27.001946602579107 23.384319859342266 74-75 22.746606625924734 26.95308959651192 26.941024266466528 23.359279511096815 76-77 22.708407647065386 26.804146713399813 26.958042288101097 23.529403351433704 78-79 22.68850779394403 26.871329541399653 26.95841223687292 23.481750427783403 80-81 22.72623038629312 26.98421662396958 26.80618374749793 23.48336924223937 82-83 22.809515932141515 26.868932038834952 26.84836799653178 23.473184032491755 84-85 22.73932438136744 26.89026616316612 26.728434851060435 23.64197460440601 86-87 22.6026776037329 27.024716780943038 26.779060614012067 23.59354500131199 88-89 22.655224559155805 26.81096507415332 26.774942286531473 23.758868080159402 90-91 22.63679931726557 26.87382590842528 26.728499541630086 23.760875232679066 92-93 22.63266071690917 26.735083647961112 26.829564693984963 23.802690941144753 94-95 22.50348111356844 26.59576470695612 26.949380815285068 23.951373364190363 96-97 22.47219148232234 26.527471963309868 26.90712240311227 24.09321415125552 98-99 22.361784536752875 26.571822983811167 26.784650839104195 24.281741640331763 100-101 22.404994694990474 26.631689731098763 26.73000353667302 24.233312037237745 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 138.0 2 156.5 3 241.5 4 199.0 5 172.5 6 265.5 7 507.5 8 831.5 9 938.5 10 798.5 11 686.0 12 724.0 13 787.5 14 884.5 15 1012.5 16 1115.5 17 1229.5 18 1368.5 19 1541.5 20 1737.5 21 1964.5 22 2316.5 23 2751.5 24 3259.5 25 3957.5 26 5019.5 27 6324.0 28 7713.5 29 9602.5 30 12112.5 31 15550.0 32 20407.0 33 25285.5 34 30092.0 35 35480.0 36 42067.0 37 48857.0 38 53219.5 39 55205.0 40 56750.0 41 58765.0 42 61154.0 43 63885.5 44 66220.5 45 68463.5 46 70239.5 47 71484.5 48 72834.5 49 72788.0 50 70809.5 51 68594.5 52 66975.5 53 65156.5 54 62641.5 55 57986.0 56 51396.5 57 45019.5 58 39546.5 59 34361.0 60 28832.5 61 23655.5 62 19102.0 63 14712.0 64 11138.5 65 8492.5 66 6265.0 67 4778.5 68 3658.5 69 2732.0 70 2077.5 71 1564.5 72 1164.0 73 907.0 74 694.5 75 544.5 76 401.0 77 260.5 78 152.5 79 89.5 80 58.0 81 30.0 82 23.5 83 20.0 84 9.0 85 4.5 86 2.5 87 2.0 88 2.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019679874048806088 2 0.003536673017466601 3 0.003251457451541875 4 0.03473925592963162 5 0.001882422735103191 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.852155659247259E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.556466977741777E-5 24-25 5.704311318494519E-4 26-27 0.002139116744435444 28-29 1.1408622636989035E-4 30-31 0.006759608912416004 32-33 0.022360900368498512 34-35 0.013348088485277174 36-37 5.133880186645066E-4 38-39 8.271251411817052E-4 40-41 0.0022532029708053347 42-43 0.008556466977741777 44-45 0.008413859194779414 46-47 0.007957514289299852 48-49 0.00981141546781057 50-51 0.19172190341460077 52-53 0.05162401743237539 54-55 0.01851049022851471 56-57 0.010866713061732057 58-59 0.02036439140702543 60-61 0.0553603413459893 62-63 0.10025327142254116 64-65 0.10444594024163463 66-67 0.10935164797553991 68-69 0.10678470788221739 70-71 0.09628877505618746 72-73 0.07358561600857928 74-75 0.24229062325305467 76-77 0.29214630417669674 78-79 0.007330040044265456 80-81 7.415604714042874E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.7112933955483554E-4 90-91 0.006217699337159025 92-93 0.018681619568069546 94-95 0.0016542502823634103 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1753060.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.45509387834858 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49799255936281 54.82411840307759 2 10.107965342681302 13.434515715339598 3 3.0013795304476667 5.983708753813604 4 1.4474145963661544 3.8475229152961927 5 0.7588328858018758 2.521415533197091 6 0.48918220560337683 1.9505189638194018 7 0.3415043368581491 1.5886291936040033 8 0.2364808461523327 1.257228546518766 9 0.18556712703525693 1.1098692763077092 >10 0.889932019218154 10.030681212692574 >50 0.03145264100809337 1.4286617579592586 >100 0.011436300289486575 1.326885250276837 >500 4.298045876444706E-4 0.15772325270978776 >1k 4.298045876444699E-4 0.5385212253876012 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2772 0.15812350974866804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2436 0.13895702371852647 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 5.7043113184945184E-5 0.0 0.0 0.0 0.0 7 5.7043113184945184E-5 0.0 0.0 5.7043113184945184E-5 0.0 8 5.7043113184945184E-5 0.0 0.0 5.7043113184945184E-5 0.0 9 5.7043113184945184E-5 0.0 0.0 5.7043113184945184E-5 0.0 10-11 5.7043113184945184E-5 0.0 0.0 5.7043113184945184E-5 0.0 12-13 5.7043113184945184E-5 0.0 0.0 5.7043113184945184E-5 3.707802357021437E-4 14-15 5.7043113184945184E-5 2.8521556592472592E-5 0.0 5.7043113184945184E-5 5.133880186645066E-4 16-17 5.7043113184945184E-5 5.7043113184945184E-5 0.0 5.7043113184945184E-5 6.27474245034397E-4 18-19 5.7043113184945184E-5 5.7043113184945184E-5 0.0 1.1408622636989037E-4 6.27474245034397E-4 20-21 5.7043113184945184E-5 5.7043113184945184E-5 0.0 1.4260778296236297E-4 6.559958016268696E-4 22-23 8.556466977741777E-5 5.7043113184945184E-5 0.0 2.8521556592472593E-4 6.845173582193421E-4 24-25 1.1408622636989037E-4 1.1408622636989037E-4 0.0 5.989526884419244E-4 6.845173582193421E-4 26-27 1.1408622636989037E-4 1.1408622636989037E-4 0.0 0.001254948490068794 7.130389148118148E-4 28-29 1.1408622636989037E-4 1.1408622636989037E-4 0.0 0.002509896980137588 7.415604714042874E-4 30-31 1.1408622636989037E-4 1.1408622636989037E-4 0.0 0.009298027449146064 7.986035845892325E-4 32-33 1.1408622636989037E-4 1.1408622636989037E-4 0.0 0.028493035035880118 7.986035845892325E-4 34-35 1.1408622636989037E-4 1.4260778296236297E-4 0.0 0.055902250921246274 9.126898109591229E-4 36-37 1.1408622636989037E-4 1.7112933955483554E-4 0.0 0.1005099654318734 9.126898109591229E-4 38-39 1.1408622636989037E-4 1.7112933955483554E-4 0.0 0.16759266653736893 9.126898109591229E-4 40-41 1.1408622636989037E-4 1.7112933955483554E-4 0.0 0.2483942363638438 9.126898109591229E-4 42-43 1.1408622636989037E-4 1.7112933955483554E-4 0.0 0.3267714738799585 9.126898109591229E-4 44-45 1.1408622636989037E-4 1.7112933955483554E-4 0.0 0.42331694294547817 9.412113675515955E-4 46-47 1.1408622636989037E-4 1.7112933955483554E-4 0.0 0.5318985088930214 9.697329241440681E-4 48-49 1.1408622636989037E-4 1.7112933955483554E-4 0.0 0.645756562810172 0.001055297593921486 50-51 1.1408622636989037E-4 1.7112933955483554E-4 0.0 0.7673724801204751 0.0011979053768838488 52-53 1.1408622636989037E-4 1.7112933955483554E-4 0.0 0.8895017854494427 0.0013690347164386843 54-55 1.4260778296236297E-4 1.7112933955483554E-4 0.0 1.0215851140291834 0.0014260778296236295 56-57 1.7112933955483554E-4 1.7112933955483554E-4 0.0 1.168528173593602 0.0014260778296236295 58-59 1.7112933955483554E-4 1.7112933955483554E-4 0.0 1.3280492396153014 0.0014260778296236295 60-61 1.7112933955483554E-4 1.7112933955483554E-4 0.0 1.505225149167741 0.0014260778296236295 62-63 1.7112933955483554E-4 1.7112933955483554E-4 0.0 1.686707813765644 0.001597207169178465 64-65 1.7112933955483554E-4 1.7112933955483554E-4 0.0 1.8727254058617504 0.001597207169178465 66-67 1.7112933955483554E-4 1.7112933955483554E-4 0.0 2.0810468552131702 0.0016257287257709376 68-69 1.7112933955483554E-4 1.7112933955483554E-4 0.0 2.307850273236512 0.0016542502823634103 70-71 1.7112933955483554E-4 1.7112933955483554E-4 0.0 2.553534961724071 0.0016827718389558829 72-73 1.7112933955483554E-4 1.7112933955483554E-4 0.0 2.811426876433208 0.0017683365087333006 74-75 1.7112933955483554E-4 1.7112933955483554E-4 0.0 3.0833228754292494 0.0018539011785107184 76-77 1.7112933955483554E-4 1.7112933955483554E-4 0.0 3.3837404310177632 0.001882422735103191 78-79 1.7112933955483554E-4 1.7112933955483554E-4 0.0 3.719153936545241 0.0019109442916956636 80-81 1.7112933955483554E-4 1.7112933955483554E-4 0.0 4.080379450788906 0.0019965089614730814 82-83 1.7112933955483554E-4 1.7112933955483554E-4 0.0 4.458118946299614 0.0020535520746580264 84-85 1.7112933955483554E-4 1.7112933955483554E-4 0.0 4.849178008739004 0.0020535520746580264 86-87 1.7112933955483554E-4 1.7112933955483554E-4 0.0 5.265022303857256 0.002167638301027917 88-89 1.7112933955483554E-4 1.7112933955483554E-4 0.0 5.709416677124571 0.0023387676405827525 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1830 0.0 53.44897 1 GTATCAA 3435 0.0 42.57427 1 ATCAACG 4445 0.0 32.252193 3 TCAACGC 4490 0.0 32.246128 4 CAACGCA 4525 0.0 31.568975 5 TATCAAC 4710 0.0 30.942396 2 AACGCAG 4865 0.0 29.55782 6 ACGCAGA 5545 0.0 25.847467 7 GTACATG 5210 0.0 25.517818 1 CGCAGAG 5755 0.0 24.821827 8 ACATGGG 5310 0.0 24.495152 3 TACATGG 5430 0.0 24.304205 2 GTGGTAT 870 0.0 24.013565 1 GCAGAGT 6585 0.0 21.18869 9 CATGGGG 3815 0.0 20.157866 4 GTGTACG 215 1.0835247E-6 19.875921 1 GAGTACA 4060 0.0 19.296616 1 AGAGTAC 6170 0.0 19.229475 10-11 GAGTACT 4060 0.0 19.053473 12-13 CAGAGTA 6345 0.0 18.437325 10-11 >>END_MODULE