FastQCFastQC Report
Sat 18 Jun 2016
SRR1930928_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930928_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085590
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54310.17601171899053342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25600.056.7236331
GTATCAA50400.043.0684621
ATCAACG60250.035.7595633
TCAACGC62800.034.532654
TATCAAC63850.033.671932
CAACGCA64750.032.715975
AACGCAG71550.030.0135486
GTACATG69600.027.7944221
TACATGG69550.027.3010772
ACATGGG70450.026.2336543
ACGCAGA83200.025.4440277
CGCAGAG85300.025.1270338
GTGGTAT12800.022.3754121
GCAGAGT91350.022.134079
CATGGGG47900.020.646034
GAGTACT57200.019.65053412-13
CAGAGTA87300.019.57511910-11
GGACCGT3557.2759576E-1219.1430686
TGGTATC14000.019.0166022
AGAGTAC83750.018.89633810-11
Produced by FastQC (version 0.11.5)