FastQCFastQC Report
Sat 18 Jun 2016
SRR1930928_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930928_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085590
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56260.18233141797840932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49280.15971013647308943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36450.057.8399051
GTATCAA70150.041.2222561
ATCAACG86450.032.7838483
CAACGCA87700.032.2493745
TCAACGC87950.032.223154
TATCAAC91750.030.891072
AACGCAG92650.030.7312666
ACGCAGA108650.025.9436667
CGCAGAG110300.025.42658
GTACATG95450.024.9232081
TACATGG96450.023.7251782
ACATGGG94050.023.471713
GTGGTAT17500.021.9780461
GCAGAGT127950.021.622359
CAGAGTA123200.019.33604410-11
CATGGGG58550.019.2965454
GAGTACA69250.019.2676471
AGAGTAC117550.019.09471310-11
GAGTACT84800.018.38290412-13
TGGTATC20200.017.861872
Produced by FastQC (version 0.11.5)