##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930928_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3085590 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27904063728493 31.0 31.0 34.0 30.0 34.0 2 31.399213116454227 31.0 31.0 34.0 30.0 34.0 3 31.480095864972338 31.0 31.0 34.0 30.0 34.0 4 35.34702731082224 37.0 35.0 37.0 33.0 37.0 5 35.144845880366475 37.0 35.0 37.0 32.0 37.0 6 35.18261013290813 37.0 35.0 37.0 32.0 37.0 7 35.06343616617892 37.0 35.0 37.0 32.0 37.0 8 35.040045501832715 37.0 35.0 37.0 32.0 37.0 9 36.55041920669953 38.0 35.0 39.0 32.0 39.0 10-11 36.47754578540895 38.0 35.0 39.0 32.0 39.0 12-13 36.37877845079871 38.0 35.0 39.0 32.0 39.0 14-15 37.40278374638238 39.0 36.0 41.0 32.0 41.0 16-17 37.37372058504208 39.0 36.0 41.0 32.0 41.0 18-19 37.3674671618718 39.0 36.0 41.0 32.0 41.0 20-21 37.33363230370853 39.0 36.0 40.0 31.5 41.0 22-23 37.20920585690257 39.0 36.0 40.0 31.0 41.0 24-25 37.090179673903535 39.0 36.0 40.0 31.0 41.0 26-27 36.82306852174139 39.0 36.0 40.0 30.5 41.0 28-29 36.6364881594768 38.5 35.0 40.0 30.0 41.0 30-31 36.39616183614803 38.0 35.0 40.0 30.0 41.0 32-33 36.15253371316345 38.0 35.0 40.0 30.0 41.0 34-35 36.33847529969957 38.0 35.0 40.0 30.0 41.0 36-37 36.3904353786472 38.0 35.0 40.0 30.0 41.0 38-39 36.341143995151654 38.0 35.0 40.0 30.0 41.0 40-41 36.238477730353026 38.0 35.0 40.0 30.0 41.0 42-43 36.086623789939686 38.0 35.0 40.0 29.5 41.0 44-45 35.882433829510724 38.0 35.0 40.0 29.0 41.0 46-47 35.650220703333886 38.0 34.0 40.0 28.0 41.0 48-49 35.434321312941776 38.0 34.0 40.0 27.0 41.0 50-51 35.15163032029531 38.0 34.0 40.0 26.5 41.0 52-53 34.94408087270182 38.0 33.5 40.0 26.0 41.0 54-55 34.77207325017258 38.0 33.0 40.0 26.0 41.0 56-57 34.51499761795961 37.5 33.0 40.0 26.0 41.0 58-59 34.271582582261416 37.0 33.0 40.0 25.5 41.0 60-61 33.97136933293147 37.0 32.5 40.0 25.0 41.0 62-63 33.67624376537388 36.0 32.0 39.5 24.0 41.0 64-65 33.37175904770238 36.0 32.0 39.0 23.0 41.0 66-67 33.03318571164672 35.5 31.5 39.0 23.0 40.0 68-69 32.66614359004275 35.0 31.0 38.5 22.0 40.0 70-71 32.269256446903185 35.0 31.0 38.0 22.0 40.0 72-73 31.850649146516552 35.0 30.5 37.5 20.5 39.5 74-75 31.32063819237164 34.0 30.0 37.0 19.5 39.0 76-77 29.958743708658638 33.5 28.5 35.5 18.0 38.0 78-79 30.720617126708348 34.0 30.0 36.0 19.0 38.5 80-81 30.718816984758185 34.0 30.0 36.0 19.0 37.5 82-83 30.43921292200195 34.0 30.0 35.0 18.0 37.0 84-85 30.119199245525166 34.0 30.0 35.0 17.5 37.0 86-87 29.78459921765367 34.0 29.0 35.0 14.5 36.0 88-89 29.44069934761261 34.0 29.0 35.0 9.0 36.0 90-91 29.103773994600708 34.0 29.0 35.0 7.0 35.5 92-93 28.75011229618971 34.0 29.0 35.0 2.0 35.0 94-95 28.337010749970023 33.0 29.0 35.0 2.0 35.0 96-97 27.884785243664908 33.0 27.5 35.0 2.0 35.0 98-99 27.399886893592473 33.0 27.0 35.0 2.0 35.0 100-101 26.1862379642143 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4222013370752009 1101 2 0.5020071117222002 1101 3 0.5609629355308776 1101 4 0.44934345172498524 1101 5 0.5194703935758156 1101 6 0.47593995743139317 1101 7 0.5066212322837842 1101 8 0.5187986962390312 1101 9 0.6277717917595922 1101 10-11 0.5466944214399874 1101 12-13 0.61012606963628 1101 14-15 0.8090135552584385 1101 16-17 0.769084439728438 1101 18-19 0.740601771441618 1101 20-21 0.7534041341817144 1101 22-23 0.8180633584903418 1101 24-25 0.6116764161284323 1101 26-27 0.6798516935735464 1101 28-29 0.6869907270278404 1101 30-31 0.6215227441563016 1101 32-33 0.757784359883928 1101 34-35 0.7218916399664934 1101 36-37 0.7326115004538067 1101 38-39 0.7871205303224755 1101 40-41 0.7939289146325663 1101 42-43 0.8115278226600466 1101 44-45 0.8301448271150136 1101 46-47 0.808364706732938 1101 48-49 0.7737424069979753 1101 50-51 0.8933837977766501 1101 52-53 0.870377345204254 1101 54-55 0.7967320628440575 1101 56-57 0.8786333150225332 1101 58-59 0.7581010843649665 1101 60-61 0.7485720811032692 1101 62-63 0.7975023882876755 1101 64-65 0.8773200377679089 1101 66-67 0.9595242667423349 1101 68-69 0.9395959289878348 1101 70-71 0.9359338792492622 1101 72-73 0.9238980317641143 1101 74-75 0.8838117185190448 1101 76-77 0.9747555712841773 1101 78-79 0.9736194861192402 1101 80-81 0.9176230508919581 1101 82-83 1.088706325989257 1101 84-85 1.14633689229262 1101 86-87 1.0375561160169617 1101 88-89 0.9490296544766537 1101 90-91 0.9146156273017745 1101 92-93 1.1060798485009862 1101 94-95 1.0017266794902362 1101 96-97 0.8879635052224124 1101 98-99 0.8850334202564731 1101 100-101 1.0798787233202596 1102 1 0.48779886988960186 1102 2 0.5338358704759543 1102 3 0.48403555509404583 1102 4 0.29875783986103244 1102 5 0.4427938268472218 1102 6 0.47316603128232515 1102 7 0.5027477004104739 1102 8 0.552227463733125 1102 9 0.7223280888413157 1102 10-11 0.7031243317705531 1102 12-13 0.7363010937145873 1102 14-15 0.8001312839919663 1102 16-17 0.750594016140532 1102 18-19 0.9106461663156367 1102 20-21 0.8679855465407371 1102 22-23 0.8640875326746738 1102 24-25 0.8246583660180562 1102 26-27 0.9051781422605245 1102 28-29 1.0307119359593315 1102 30-31 1.023676153612179 1102 32-33 0.9280789562659919 1102 34-35 0.9678616203063868 1102 36-37 1.0363107813163737 1102 38-39 1.0057914211076593 1102 40-41 1.0336761155120229 1102 42-43 1.0198615719755608 1102 44-45 1.0292371791146806 1102 46-47 0.9367153019139707 1102 48-49 0.9529118340595915 1102 50-51 0.9123676547169595 1102 52-53 0.9829976448960238 1102 54-55 0.861993921585551 1102 56-57 0.7767886334127851 1102 58-59 0.9569438941490915 1102 60-61 0.8900899721357831 1102 62-63 0.8069993085439862 1102 64-65 0.8371248099531456 1102 66-67 0.8604755033961382 1102 68-69 0.92717335112021 1102 70-71 0.871268868599806 1102 72-73 0.8663252661205654 1102 74-75 1.0538566785009316 1102 76-77 1.20834196619926 1102 78-79 1.2455667687132994 1102 80-81 1.1071328687934816 1102 82-83 1.2352895142939317 1102 84-85 1.3106596641994521 1102 86-87 1.2579577909213313 1102 88-89 1.2621143409764706 1102 90-91 1.1823053872270002 1102 92-93 1.1811775509419356 1102 94-95 1.19597742274836 1102 96-97 1.2905302910828915 1102 98-99 1.2263412889502696 1102 100-101 1.4570664206959556 1103 1 0.4464936262885999 1103 2 0.49624550027851555 1103 3 0.4632032085307891 1103 4 0.3092077115356773 1103 5 0.3181823178350527 1103 6 0.40571345568606176 1103 7 0.47620488033049924 1103 8 0.49947225503803594 1103 9 0.5676000451696979 1103 10-11 0.5578591244814461 1103 12-13 0.5371174747280847 1103 14-15 0.6481822344645067 1103 16-17 0.6783004791740481 1103 18-19 0.5986604061332486 1103 20-21 0.7633053733085333 1103 22-23 0.6197474117985351 1103 24-25 0.7256652646407531 1103 26-27 0.6966721904690729 1103 28-29 0.6406446121864775 1103 30-31 0.8044416631571565 1103 32-33 0.6976983946972481 1103 34-35 0.7003008728685458 1103 36-37 0.7072106369936435 1103 38-39 0.6822910136379221 1103 40-41 0.6723274047282928 1103 42-43 0.7463344772506844 1103 44-45 0.8055895096674561 1103 46-47 0.7379141596882093 1103 48-49 0.6809449281280919 1103 50-51 0.709542456331711 1103 52-53 0.7137175773644415 1103 54-55 0.7726297147830081 1103 56-57 0.7794921805992416 1103 58-59 0.7299985093218737 1103 60-61 0.624028709580557 1103 62-63 0.6865325146596533 1103 64-65 0.6230285755800722 1103 66-67 0.675367281943025 1103 68-69 0.6241938140949443 1103 70-71 0.5937065317210894 1103 72-73 0.6722952079028452 1103 74-75 0.6435157930456974 1103 76-77 0.7340841686251345 1103 78-79 0.8714870390642702 1103 80-81 0.8309578292306306 1103 82-83 0.7578552219480201 1103 84-85 0.7350026063799326 1103 86-87 0.7857448305568191 1103 88-89 0.7391401339137005 1103 90-91 0.7968686438846326 1103 92-93 0.7988313246658372 1103 94-95 0.9158821836011661 1103 96-97 0.8936226147574509 1103 98-99 0.9649749145922009 1103 100-101 1.0724667079934775 1104 1 0.38342021130644355 1104 2 0.45719475925134745 1104 3 0.40522356566224005 1104 4 0.16256546268957806 1104 5 0.3525347110452728 1104 6 0.30673964282672017 1104 7 0.3587231370017818 1104 8 0.35598044190378886 1104 9 0.4800255039410217 1104 10-11 0.4800476387570214 1104 12-13 0.4714072035542074 1104 14-15 0.502869590559726 1104 16-17 0.5019692891107539 1104 18-19 0.654318134563205 1104 20-21 0.6049332218421455 1104 22-23 0.49942554641773995 1104 24-25 0.6660235346039158 1104 26-27 0.6505027939215395 1104 28-29 0.5948460647598637 1104 30-31 0.5544049392363419 1104 32-33 0.5806149450830702 1104 34-35 0.55872893170271 1104 36-37 0.6491214934362048 1104 38-39 0.5540195648549471 1104 40-41 0.6258331623078419 1104 42-43 0.5729252802110025 1104 44-45 0.5927698761846827 1104 46-47 0.46291262084717033 1104 48-49 0.39304179227087843 1104 50-51 0.33530598760396657 1104 52-53 0.3178567126607348 1104 54-55 0.47212020798503573 1104 56-57 0.4964361394795489 1104 58-59 0.37456325333971563 1104 60-61 0.1923518634225445 1104 62-63 0.23690718195759075 1104 64-65 0.31379173429080964 1104 66-67 0.29709381993944817 1104 68-69 0.2874683660145152 1104 70-71 0.21056075187116363 1104 72-73 0.30911682776329386 1104 74-75 0.44207030064665176 1104 76-77 0.5485646859719218 1104 78-79 0.49901054167937176 1104 80-81 0.36909230033443663 1104 82-83 0.48990504614989305 1104 84-85 0.599807159683305 1104 86-87 0.46410775360504886 1104 88-89 0.5482110117750736 1104 90-91 0.5574203959187969 1104 92-93 0.5262828141482636 1104 94-95 0.6850101165974785 1104 96-97 0.7193914220561375 1104 98-99 0.8645542209057986 1104 100-101 1.0105900716732172 1105 1 0.15362596166653475 1105 2 0.2565028722154885 1105 3 0.25692355656084587 1105 4 0.0469925254344048 1105 5 0.08602996767966431 1105 6 0.1681664424914615 1105 7 0.19885068329776345 1105 8 0.21122427095516372 1105 9 0.21970853092869191 1105 10-11 0.23071559501419614 1105 12-13 0.2230347908291037 1105 14-15 0.33084283699059824 1105 16-17 0.30589660836998434 1105 18-19 0.2965009657124327 1105 20-21 0.27016589829032256 1105 22-23 0.34158972197410975 1105 24-25 0.4274531869442626 1105 26-27 0.2883664930810852 1105 28-29 0.38173910708405856 1105 30-31 0.4837595145884137 1105 32-33 0.4836406377285627 1105 34-35 0.5115416472099028 1105 36-37 0.4491547293710454 1105 38-39 0.3433975593153278 1105 40-41 0.39687791626003843 1105 42-43 0.30941448781469205 1105 44-45 0.531670319164455 1105 46-47 0.4395408307298325 1105 48-49 0.4612944560133272 1105 50-51 0.5074218986059194 1105 52-53 0.37216382266036163 1105 54-55 0.5179785531705576 1105 56-57 0.5608115189872862 1105 58-59 0.5597831787807337 1105 60-61 0.4915683285227743 1105 62-63 0.4310077129627743 1105 64-65 0.4412692943747274 1105 66-67 0.43364284316973567 1105 68-69 0.49560935882713153 1105 70-71 0.28199600541741887 1105 72-73 0.23489215556062604 1105 74-75 0.4959785341476568 1105 76-77 0.49453903996541726 1105 78-79 0.47899073596016706 1105 80-81 0.5428307951088343 1105 82-83 0.6114258573240505 1105 84-85 0.5848307938742998 1105 86-87 0.5366543733429374 1105 88-89 0.6008345903615329 1105 90-91 0.40336011027993735 1105 92-93 0.5568197103375887 1105 94-95 0.37592986902145853 1105 96-97 0.2780979917680604 1105 98-99 0.6366500273368167 1105 100-101 0.935052398922199 1106 1 0.05623227403700426 1106 2 0.1706826474557488 1106 3 0.14667807146826917 1106 4 0.07115229872149342 1106 5 0.11265074077505233 1106 6 0.08915800405443974 1106 7 0.10569836824349466 1106 8 0.1939526928987192 1106 9 0.19989411867143758 1106 10-11 0.1813442278282409 1106 12-13 0.06970083055088594 1106 14-15 0.15561609694726286 1106 16-17 0.02282601919852567 1106 18-19 0.17962978841844546 1106 20-21 0.1960279173663224 1106 22-23 0.14766056795183857 1106 24-25 0.196400245065675 1106 26-27 0.2648781687239179 1106 28-29 0.17525275941177654 1106 30-31 0.17138348691121053 1106 32-33 0.29192693512468537 1106 34-35 0.12837493653652388 1106 36-37 0.2759203176925027 1106 38-39 0.19972909982742237 1106 40-41 0.21194480344251332 1106 42-43 0.21327218820496796 1106 44-45 0.11921729481849042 1106 46-47 0.06820710193895962 1106 48-49 0.17935105647702443 1106 50-51 0.20616612498607623 1106 52-53 0.06013805522557192 1106 54-55 0.3144351691873055 1106 56-57 0.2973806414694167 1106 58-59 0.2956539509718752 1106 60-61 0.09218149870575587 1106 62-63 0.11149762517843698 1106 64-65 0.14338772004378342 1106 66-67 0.1513243165866669 1106 68-69 0.10280323490182042 1106 70-71 -0.004259388418503818 1106 72-73 0.020398606461792923 1106 74-75 0.27879548191470604 1106 76-77 0.12241074895689152 1106 78-79 0.15222157586135054 1106 80-81 0.20109747033570713 1106 82-83 0.377271934517843 1106 84-85 0.22212584619405717 1106 86-87 0.1777987165518553 1106 88-89 0.156719025731352 1106 90-91 0.16102781200849492 1106 92-93 0.2915895160690667 1106 94-95 0.2797817370158029 1106 96-97 0.3125250425108099 1106 98-99 0.19930112708254555 1106 100-101 0.20191010292946387 1107 1 0.09894139828667647 1107 2 0.17676727132578307 1107 3 0.17043278115123428 1107 4 0.07831495648691345 1107 5 0.1906248249834661 1107 6 0.20351841836328077 1107 7 0.09831473143704983 1107 8 0.061112771978415026 1107 9 0.04650744474624702 1107 10-11 0.17400894137924183 1107 12-13 0.13555151032109336 1107 14-15 0.014778131530306382 1107 16-17 0.18353723504885266 1107 18-19 0.1896451172725122 1107 20-21 0.2500750213589882 1107 22-23 0.22789601628500833 1107 24-25 0.18288936444449888 1107 26-27 0.1544318053870981 1107 28-29 0.2630125978141393 1107 30-31 0.23918913280035525 1107 32-33 0.28921985233454706 1107 34-35 0.245739161569837 1107 36-37 0.27091892090439984 1107 38-39 0.29760025149711566 1107 40-41 0.20378451663535913 1107 42-43 0.34600108237857086 1107 44-45 0.2828689287024204 1107 46-47 0.2825800515256489 1107 48-49 0.4142243785405455 1107 50-51 0.3373884578371005 1107 52-53 0.22301894859690918 1107 54-55 0.3837015330971667 1107 56-57 0.35607654279170475 1107 58-59 0.2905284549330247 1107 60-61 0.2198361124043089 1107 62-63 0.25987824635517853 1107 64-65 0.28517550137123493 1107 66-67 0.15938378394480424 1107 68-69 0.28197945225637966 1107 70-71 0.3255761845511316 1107 72-73 0.2827089656154165 1107 74-75 0.322418248844496 1107 76-77 0.338741708944287 1107 78-79 0.38717172127057253 1107 80-81 0.5656203060304499 1107 82-83 0.49450346468130135 1107 84-85 0.4837729100738315 1107 86-87 0.42479418952579806 1107 88-89 0.4459270468765766 1107 90-91 0.3851168554593478 1107 92-93 0.36410054711357986 1107 94-95 0.4269510922011115 1107 96-97 0.4885934063347115 1107 98-99 0.23415152749513268 1107 100-101 0.569742431052898 1108 1 0.22398791197336365 1108 2 0.32399590046779636 1108 3 0.3033354702943427 1108 4 0.0982684556569211 1108 5 0.2792170058352923 1108 6 0.215293314527095 1108 7 0.35713488353543 1108 8 0.3319276697291187 1108 9 0.3411823485138825 1108 10-11 0.3894535883472443 1108 12-13 0.4330928602079709 1108 14-15 0.41493413834361803 1108 16-17 0.435360773600749 1108 18-19 0.6118278112791202 1108 20-21 0.5240631351605742 1108 22-23 0.5662812043435608 1108 24-25 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2316 68-69 -0.9172012960825953 2316 70-71 -0.7668401479335714 2316 72-73 -0.7364539792512836 2316 74-75 -0.3372963209945148 2316 76-77 -0.8642590009703319 2316 78-79 -0.754209604823977 2316 80-81 -0.7897632054148396 2316 82-83 -1.0208505241398598 2316 84-85 -0.9178803985824011 2316 86-87 -0.6765036057537301 2316 88-89 -0.7890616417554313 2316 90-91 -0.4297572690796905 2316 92-93 -0.750012550811082 2316 94-95 -0.5124718056036208 2316 96-97 -0.38177859448456175 2316 98-99 -0.30523819153252774 2316 100-101 -0.5354111227370346 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 18.0 4 55.0 5 116.0 6 194.0 7 583.0 8 1355.0 9 2360.0 10 3945.0 11 6089.0 12 8510.0 13 11155.0 14 13186.0 15 16270.0 16 19017.0 17 21761.0 18 19270.0 19 16591.0 20 16750.0 21 17759.0 22 19749.0 23 23155.0 24 28101.0 25 35316.0 26 45510.0 27 58044.0 28 73427.0 29 91463.0 30 112723.0 31 136511.0 32 163289.0 33 198209.0 34 244580.0 35 304679.0 36 393808.0 37 496010.0 38 415100.0 39 70906.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.72194856940589 20.93820056453589 14.443801191048852 23.89604967500937 2 15.521808360484604 21.36541639564011 39.75905293517304 23.353722308702245 3 18.457938473106292 26.006002307582616 30.684464005600425 24.85159521371067 4 12.822197806686667 17.653620925144196 38.41841794461895 31.10576332355019 5 12.734012115932334 38.417234803553626 35.688913779312685 13.159839301201352 6 30.813274224143267 38.54379180117637 17.389419005577217 13.253514969103145 7 26.52896204615649 33.00992678871788 22.777685953091627 17.683425212034003 8 25.152985328575735 36.44440771456999 20.431392375526237 17.97121458132804 9 25.5267956944363 15.898941822776786 20.869726979192627 37.704535503594286 10-11 24.289341746635166 26.839810214578087 28.927644307895733 19.943203730891014 12-13 24.822529888935343 24.291934443655833 29.019085490943386 21.86645017646544 14-15 21.98623634154835 26.007461459147436 26.51689409754603 25.489408101758187 16-17 21.568095566812183 28.95737930185151 27.45377707342842 22.020748057907888 18-19 21.526677231907026 27.864849186055178 29.349071004248785 21.259402577789015 20-21 22.279531629283216 27.10238560534614 29.298756477691462 21.31932628767918 22-23 21.906932208577974 27.052901884712792 29.14063909019654 21.899526816512697 24-25 21.83833486816615 27.21158930304438 29.263139268131866 21.686936560657603 26-27 21.759101713229352 27.42654555896287 29.022971183444724 21.791381544363052 28-29 21.71279833859867 27.46121646830361 29.127786989060105 21.69819820403761 30-31 21.99453970334452 27.20243498051364 29.04878144827992 21.754243867861923 32-33 21.829979297314583 27.410643997282865 28.857206254211082 21.90217045119147 34-35 22.0370498223259 27.40334888852802 28.956210457667602 21.603390831478475 36-37 22.124710912221023 27.15922967829586 28.901340045009544 21.814719364473575 38-39 21.852335525095125 27.28698867421395 28.988862509821935 21.871813290868992 40-41 21.991584584550843 27.279950141434465 29.025324028820997 21.70314124519369 42-43 22.05825775777771 27.27038627722284 28.693349124224593 21.978006840774857 44-45 21.951527025899196 27.4984260179835 28.64614858552293 21.90389837059437 46-47 22.14076533854205 27.455705456172502 28.362866270351685 22.04066293493376 48-49 22.156519591486223 27.539918156576636 28.252332890856152 22.05122936108099 50-51 22.160255008344468 27.647454503211556 28.1108285514398 22.081461937004185 52-53 22.230121293527418 27.630459647589735 28.100588593398772 22.038830465484075 54-55 22.116582404406337 27.57176554637893 28.208748688416346 22.10290336079839 56-57 22.12110290142368 27.758760733348804 27.96845241413645 22.151683951091066 58-59 22.011878013325042 27.701495991741826 28.240873779373914 22.045752215559215 60-61 21.981547125712233 27.790499499994166 28.17505327558947 22.052900098704136 62-63 21.973953883074696 27.723533957075098 28.155900406333956 22.14661175351625 64-65 21.788306297257982 27.62015384675275 28.14480098931144 22.446738866677826 66-67 21.81837882870637 27.797039226741948 27.994318133892715 22.390263810658965 68-69 22.048124351276005 27.668347070318234 28.03251653247606 22.2510120459297 70-71 21.917190113313602 27.41031448319889 28.104754422318614 22.567740981168896 72-73 21.967640781475552 27.577641141462045 28.034840206534955 22.41987787052745 74-75 21.88129441882883 27.744868172867942 27.836408587676793 22.537428820626435 76-77 21.822124875621405 27.68944635637763 27.921072537628895 22.567356230372074 78-79 21.861229339552803 27.729483458029584 27.83621316642056 22.57307403599705 80-81 21.882395835443294 27.743628592841663 27.745783791448076 22.628191780266967 82-83 21.994675248493806 27.609047216253618 27.899445486924705 22.49683204832787 84-85 22.029093301443158 27.631749519540833 27.65898904261422 22.68016813640179 86-87 21.72297680508428 27.77360245528408 27.745957823301215 22.757462916330425 88-89 21.834215607667392 27.596524614773337 27.705774527107657 22.863485250451614 90-91 21.744640985705026 27.636303470020195 27.800966848388963 22.818088695885812 92-93 21.846218077577674 27.444567639173894 27.83472990343431 22.874484379814124 94-95 21.54941609953256 27.40191920838569 27.956910663698416 23.091754028383335 96-97 21.616028052981765 27.285835123914715 27.898068116632473 23.200068706471047 98-99 21.48485378809239 27.40078882806854 27.78700345800965 23.32735392582942 100-101 21.463075133118785 27.344397667869032 27.771836180438747 23.420691018573432 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 232.0 1 180.5 2 153.0 3 194.5 4 185.5 5 217.5 6 359.0 7 677.0 8 1072.0 9 1295.0 10 1243.0 11 1161.5 12 1222.0 13 1389.5 14 1623.0 15 1858.5 16 2145.5 17 2456.5 18 2815.0 19 3180.5 20 3820.0 21 4740.0 22 5754.5 23 7526.5 24 10043.5 25 13108.5 26 17421.5 27 23029.0 28 28433.5 29 35177.0 30 42840.0 31 50225.0 32 59794.5 33 68201.0 34 74247.5 35 80304.5 36 89528.0 37 98431.0 38 101218.0 39 102394.0 40 104900.5 41 105853.5 42 106045.0 43 108317.0 44 110269.5 45 113088.5 46 114890.0 47 114163.5 48 113270.0 49 111725.5 50 107969.5 51 103201.0 52 100529.5 53 98223.0 54 94510.5 55 86836.0 56 77191.5 57 67661.5 58 58980.5 59 51861.5 60 43689.0 61 35519.0 62 28647.5 63 21938.0 64 16212.5 65 12209.0 66 9069.0 67 6926.5 68 5346.5 69 4058.0 70 3157.0 71 2421.5 72 1853.0 73 1483.5 74 1138.5 75 852.0 76 660.0 77 426.0 78 248.5 79 173.5 80 116.0 81 69.5 82 43.0 83 28.0 84 15.5 85 7.5 86 6.0 87 4.0 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018732235974319337 2 0.0032084625630754574 3 0.003564958403417175 4 0.0334782002793631 5 0.0021389750420503046 6 3.240871275833795E-5 7 0.0 8 0.0 9 3.240871275833795E-5 10-11 0.0 12-13 0.0 14-15 1.6204356379168976E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.6204356379168976E-5 24-25 6.64378611545928E-4 26-27 0.0023334273186003326 28-29 6.48174255166759E-5 30-31 0.0073405734397635454 32-33 0.023480112393415846 34-35 0.014486694602977064 36-37 7.129916806834349E-4 38-39 6.64378611545928E-4 40-41 0.0020093401910169528 42-43 0.008653126306476234 44-45 0.008507287099063713 46-47 0.008085973833205319 48-49 0.00999808788594726 50-51 0.19459811575744024 52-53 0.04999043942973629 54-55 0.018764644687077673 56-57 0.011213414614384931 58-59 0.020952232798265486 60-61 0.052696566945057505 62-63 0.09411490185021341 64-65 0.09858730421086405 66-67 0.10215226261428123 68-69 0.10035357905619348 70-71 0.09132775255299636 72-73 0.06938705401560157 74-75 0.2500170145741981 76-77 0.3013038025142679 78-79 0.007324369083384377 80-81 8.912396008542938E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 1.296348510333518E-4 90-91 0.006627581759080112 92-93 0.0191697535965569 94-95 0.0015232094996418836 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3085590.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.14674034768481 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04271665114011 44.02740124142957 2 13.183177357652276 15.067512268304641 3 4.189451912907346 7.182405619980827 4 1.8697667878090094 4.274043085345846 5 0.9566513667785081 2.73347536302746 6 0.5970348108355039 2.04711559880074 7 0.38824457901337445 1.5530838503791458 8 0.2958740128488962 1.3526588310322745 9 0.21162277829378998 1.0884196766529797 >10 1.1378183195888731 11.781476781068028 >50 0.08532348920509934 3.3380377851440706 >100 0.039757153500200815 4.0693090001271965 >500 0.0019917181101416527 0.7826276791634844 >1k 5.121560851284302E-4 0.5194753818977401 >5k 5.6906231680936677E-5 0.18295783764596757 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5626 0.18233141797840932 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4928 0.15971013647308943 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.48174255166759E-5 7 0.0 0.0 0.0 0.0 6.48174255166759E-5 8 0.0 0.0 0.0 0.0 6.48174255166759E-5 9 0.0 0.0 0.0 0.0 6.48174255166759E-5 10-11 0.0 0.0 0.0 1.6204356379168976E-5 6.48174255166759E-5 12-13 0.0 0.0 0.0 3.240871275833795E-5 2.2686098930836565E-4 14-15 0.0 1.6204356379168976E-5 0.0 3.240871275833795E-5 2.754740584458726E-4 16-17 0.0 3.240871275833795E-5 0.0 3.240871275833795E-5 2.916784148250416E-4 18-19 0.0 3.240871275833795E-5 0.0 4.861306913750693E-5 3.078827712042105E-4 20-21 0.0 3.240871275833795E-5 0.0 8.102178189584489E-5 3.5649584034171746E-4 22-23 0.0 3.240871275833795E-5 0.0 1.458392074125208E-4 3.889045531000554E-4 24-25 0.0 3.240871275833795E-5 0.0 4.051089094792244E-4 4.213132658583934E-4 26-27 0.0 3.240871275833795E-5 0.0 7.616047498209418E-4 4.537219786167313E-4 28-29 0.0 3.240871275833795E-5 0.0 0.0024792665260128534 4.537219786167313E-4 30-31 0.0 3.240871275833795E-5 0.0 0.008507287099063711 4.699263349959003E-4 32-33 0.0 3.240871275833795E-5 0.0 0.023966243084790916 5.347437605125762E-4 34-35 0.0 3.240871275833795E-5 0.0 0.046441685382698286 6.157655424084211E-4 36-37 0.0 3.240871275833795E-5 0.0 0.08199404327859502 6.157655424084211E-4 38-39 0.0 6.48174255166759E-5 0.0 0.13272988310177308 6.48174255166759E-4 40-41 0.0 1.296348510333518E-4 0.0 0.18712790746664332 6.48174255166759E-4 42-43 0.0 2.2686098930836565E-4 0.0 0.244345489841489 6.48174255166759E-4 44-45 0.0 2.2686098930836565E-4 0.0 0.31446173989415316 6.48174255166759E-4 46-47 0.0 2.2686098930836565E-4 0.0 0.39739563584273996 6.48174255166759E-4 48-49 0.0 2.2686098930836565E-4 0.0 0.48235507633872293 7.454003934417729E-4 50-51 0.0 2.2686098930836565E-4 0.0 0.5720623932538024 8.102178189584488E-4 52-53 0.0 2.2686098930836565E-4 0.0 0.6573135121646103 9.074439572334626E-4 54-55 0.0 2.2686098930836565E-4 0.0 0.7565976036997787 9.398526699918006E-4 56-57 0.0 2.2686098930836565E-4 0.0 0.8767529062513166 9.398526699918006E-4 58-59 0.0 2.4306534568753463E-4 0.0 1.005869217880535 9.722613827501385E-4 60-61 3.240871275833795E-5 3.078827712042105E-4 0.0 1.1407542803807376 9.722613827501385E-4 62-63 3.240871275833795E-5 3.240871275833795E-4 0.0 1.2782482442579863 0.0011018962337834904 64-65 3.240871275833795E-5 3.240871275833795E-4 0.0 1.4299858373925245 0.0011343049465418285 66-67 3.240871275833795E-5 3.240871275833795E-4 0.0 1.5995482225441489 0.0011829180156793353 68-69 3.240871275833795E-5 3.240871275833795E-4 0.0 1.7889771486166341 0.0011991223720585042 70-71 3.240871275833795E-5 3.240871275833795E-4 0.0 1.9980781633334306 0.0012315310848168423 72-73 4.861306913750693E-5 3.240871275833795E-4 0.0 2.214665590697403 0.0012801441539543493 74-75 6.48174255166759E-5 3.240871275833795E-4 0.0 2.4389824960542392 0.001344961579471025 76-77 6.48174255166759E-5 3.5649584034171746E-4 0.0 2.692402425468063 0.0013773702922293628 78-79 6.48174255166759E-5 3.5649584034171746E-4 0.0 2.9757680054705906 0.001393574648608532 80-81 6.48174255166759E-5 3.5649584034171746E-4 0.0 3.2847364685522056 0.001409779004987701 82-83 6.48174255166759E-5 3.7270019672088644E-4 0.0 3.6088560048483433 0.0014908007868835458 84-85 6.48174255166759E-5 3.889045531000554E-4 0.0 3.9442375688280036 0.0015070051432627147 86-87 6.48174255166759E-5 3.889045531000554E-4 0.0 4.317002582974407 0.0016204356379168976 88-89 6.48174255166759E-5 3.889045531000554E-4 0.0 4.7178011336567724 0.0017500704889502495 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3645 0.0 57.839905 1 GTATCAA 7015 0.0 41.222256 1 ATCAACG 8645 0.0 32.783848 3 CAACGCA 8770 0.0 32.249374 5 TCAACGC 8795 0.0 32.22315 4 TATCAAC 9175 0.0 30.89107 2 AACGCAG 9265 0.0 30.731266 6 ACGCAGA 10865 0.0 25.943666 7 CGCAGAG 11030 0.0 25.4265 8 GTACATG 9545 0.0 24.923208 1 TACATGG 9645 0.0 23.725178 2 ACATGGG 9405 0.0 23.47171 3 GTGGTAT 1750 0.0 21.978046 1 GCAGAGT 12795 0.0 21.62235 9 CAGAGTA 12320 0.0 19.336044 10-11 CATGGGG 5855 0.0 19.296545 4 GAGTACA 6925 0.0 19.267647 1 AGAGTAC 11755 0.0 19.094713 10-11 GAGTACT 8480 0.0 18.382904 12-13 TGGTATC 2020 0.0 17.86187 2 >>END_MODULE