FastQCFastQC Report
Sat 18 Jun 2016
SRR1930927_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930927_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427886
Sequences flagged as poor quality0
Sequence length101
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7780.18182413072640843No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3250.055.717081
GTATCAA6150.050.7090841
ATACTAG500.00121803640.240111
GTGGTAT1052.8016075E-838.3239171
TCAACGC8600.036.217674
ATCAACG8650.034.8576283
TATCAAC9050.034.4382632
CAACGCA9050.034.153425
TGGTATC1209.12205E-833.5125052
AACGCAG9800.031.8733656
GTACATG14700.031.8225381
CTAGTAC803.2248851E-431.4081763
TACATGG14600.031.3318982
GTACACG854.5752747E-429.588321
CTATACC854.6088413E-429.551424
ACATGGG16100.029.0281773
GTAAGGT1054.704124E-528.7070944
CCTAGTA750.00890262826.8100032
ACGCAGA11900.026.2671057
AGTACAT9800.025.1343782
Produced by FastQC (version 0.11.5)