##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930927_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 427886 Sequences flagged as poor quality 0 Sequence length 101 %GC 53 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.44083003416798 30.0 16.0 31.0 2.0 33.0 2 22.25003388753079 30.0 10.0 31.0 2.0 33.0 3 22.742368294358776 30.0 16.0 31.0 2.0 34.0 4 24.7083452134447 33.0 10.0 35.0 2.0 37.0 5 24.65996316775964 33.0 10.0 35.0 2.0 37.0 6 25.447429922923394 33.0 17.0 35.0 2.0 37.0 7 25.532436209644626 33.0 17.0 35.0 2.0 37.0 8 26.34273848641928 33.0 17.0 35.0 2.0 37.0 9 27.57884810440164 34.0 17.0 37.0 2.0 39.0 10-11 27.845807060759174 34.5 22.5 37.0 2.0 39.0 12-13 27.770226181739996 34.5 22.5 37.0 2.0 39.0 14-15 28.05766372351514 34.5 18.5 38.0 2.0 40.0 16-17 27.12879481918081 34.0 16.5 38.0 2.0 40.0 18-19 26.462415222746245 33.0 10.5 38.0 2.0 40.0 20-21 25.90092805092945 33.0 10.0 38.0 2.0 40.0 22-23 25.82994535927794 32.5 10.0 38.0 2.0 40.0 24-25 25.48715779436579 32.0 9.5 38.0 2.0 40.0 26-27 25.114217338262996 32.0 8.5 38.0 2.0 40.0 28-29 25.027799460603994 32.0 8.5 38.0 2.0 40.0 30-31 24.953216510939832 32.0 8.0 38.0 2.0 40.0 32-33 24.62831221400093 31.0 5.0 38.0 2.0 40.0 34-35 21.59193687103574 26.5 2.0 36.5 2.0 39.0 36-37 19.901430521213594 19.0 2.0 35.0 2.0 38.5 38-39 18.870757631705644 19.0 2.0 34.0 2.0 38.0 40-41 20.186937642269204 23.5 2.0 35.0 2.0 38.0 42-43 20.05103111576448 23.5 2.0 35.0 2.0 38.0 44-45 18.030614462730725 17.0 2.0 34.0 2.0 38.0 46-47 18.768115806546604 18.5 2.0 33.0 2.0 38.0 48-49 18.263883370804372 19.0 2.0 33.5 2.0 38.0 50-51 16.150902343147475 9.5 2.0 31.5 2.0 37.5 52-53 18.963836161968374 22.5 2.0 33.0 2.0 37.5 54-55 20.63230042581435 26.5 2.0 34.5 2.0 38.5 56-57 20.80678031064349 28.0 2.0 35.0 2.0 39.0 58-59 20.394326993638494 26.5 2.0 35.0 2.0 38.0 60-61 20.01121326708516 26.0 2.0 34.5 2.0 38.0 62-63 19.483038706571378 25.5 2.0 34.0 2.0 37.5 64-65 18.954635113090873 24.5 2.0 34.0 2.0 36.5 66-67 18.512053444141664 23.0 2.0 33.0 2.0 36.0 68-69 18.01504022099344 21.5 2.0 33.0 2.0 35.5 70-71 17.411601454593047 20.0 2.0 32.5 2.0 35.0 72-73 16.936728474406735 18.0 2.0 32.0 2.0 35.0 74-75 16.406889919277564 14.0 2.0 31.5 2.0 34.5 76-77 15.853208564898129 8.5 2.0 31.0 2.0 34.0 78-79 15.37126711320305 7.0 2.0 31.0 2.0 34.0 80-81 14.874347372898388 2.0 2.0 30.5 2.0 34.0 82-83 14.415504363311722 2.0 2.0 30.0 2.0 34.0 84-85 13.91726885198394 2.0 2.0 30.0 2.0 34.0 86-87 13.344782255086635 2.0 2.0 29.0 2.0 34.0 88-89 12.802422841597995 2.0 2.0 29.0 2.0 34.0 90-91 12.216694399910256 2.0 2.0 28.0 2.0 33.5 92-93 11.572356889451864 2.0 2.0 27.0 2.0 33.0 94-95 10.899345853802181 2.0 2.0 25.0 2.0 33.0 96-97 10.169791720224545 2.0 2.0 24.5 2.0 32.5 98-99 9.363652935594995 2.0 2.0 19.0 2.0 32.0 100-101 8.319435550590578 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.386058967565674 1101 2 7.590413059842586 1101 3 6.9865910875688115 1101 4 9.028241789292814 1101 5 8.970651287946346 1101 6 8.391104236563095 1101 7 7.792627737902951 1101 8 7.116940064686002 1101 9 7.575332268449795 1101 10-11 6.53639723546819 1101 12-13 6.01593583255314 1101 14-15 6.7185059276327515 1101 16-17 7.62742546511917 1101 18-19 7.781260195696767 1101 20-21 8.314892039279854 1101 22-23 8.122649709190057 1101 24-25 8.205031421513688 1101 26-27 8.188148443469505 1101 28-29 8.186841015840947 1101 30-31 7.940927771753788 1101 32-33 7.301843073446552 1101 34-35 9.917348785090535 1101 36-37 4.2589628407440365 1101 38-39 7.344368163612248 1101 40-41 8.151945958811432 1101 42-43 9.372723160015955 1101 44-45 3.410862128323224 1101 46-47 4.2786847497906635 1101 48-49 8.591738319619985 1101 50-51 -4.854060223594134 1101 52-53 4.4662734276478595 1101 54-55 7.445546166079296 1101 56-57 7.505592524213018 1101 58-59 7.6669816431792555 1101 60-61 7.565870826735143 1101 62-63 7.89373721690993 1101 64-65 7.702074020985396 1101 66-67 7.6191429923562275 1101 68-69 7.360050257466696 1101 70-71 7.409181985072017 1101 72-73 7.254768121633955 1101 74-75 7.341425758859032 1101 76-77 7.72592582578992 1101 78-79 7.696214696071793 1101 80-81 7.624887455436927 1101 82-83 7.269133969825338 1101 84-85 7.3024673006030145 1101 86-87 7.5251499459694475 1101 88-89 7.13241356057174 1101 90-91 6.643858954402965 1101 92-93 6.537785660600891 1101 94-95 5.9488431988773804 1101 96-97 5.738845208402482 1101 98-99 5.309577960715034 1101 100-101 4.80430976886961 1102 1 6.359174895385433 1102 2 7.814485541754788 1102 3 7.511602274600904 1102 4 9.383934103173281 1102 5 8.91034187223623 1102 6 8.521140286459769 1102 7 8.585559544772043 1102 8 7.014748411971706 1102 9 7.24953069874579 1102 10-11 6.92266157120989 1102 12-13 6.502730106803867 1102 14-15 6.854261190565712 1102 16-17 7.620338668833764 1102 18-19 7.894586087768303 1102 20-21 8.61048862233288 1102 22-23 8.336269229429789 1102 24-25 8.544870831039503 1102 26-27 8.276734654006933 1102 28-29 8.19582835061226 1102 30-31 7.843469867675676 1102 32-33 8.2441205017867 1102 34-35 10.18905288432077 1102 36-37 0.765986788534537 1102 38-39 3.482971720334273 1102 40-41 8.769236333941524 1102 42-43 10.044822450582146 1102 44-45 1.1897988323470372 1102 46-47 1.6324589276066526 1102 48-49 8.13414848398137 1102 50-51 -5.157143319360156 1102 52-53 5.137641161733363 1102 54-55 8.056976547544522 1102 56-57 8.634695902445316 1102 58-59 8.909961466298451 1102 60-61 8.643159391828583 1102 62-63 8.92379639505273 1102 64-65 8.899076399172444 1102 66-67 8.442556319253875 1102 68-69 8.853805631013014 1102 70-71 8.714537682413777 1102 72-73 8.355622358376696 1102 74-75 8.363621333319415 1102 76-77 8.029735450157036 1102 78-79 7.973492199830135 1102 80-81 7.903296232103543 1102 82-83 7.927240671486048 1102 84-85 7.239553443264073 1102 86-87 7.094348652798816 1102 88-89 7.273478264354216 1102 90-91 7.77403578492823 1102 92-93 7.445061505158966 1102 94-95 6.854542803183257 1102 96-97 6.098137484071065 1102 98-99 6.037079456509211 1102 100-101 5.641313454305351 1103 1 6.752112258006939 1103 2 7.560290237033055 1103 3 7.356569592347437 1103 4 9.214433691939632 1103 5 9.41230184549402 1103 6 8.133604273454203 1103 7 8.20092381121297 1103 8 7.392044466533509 1103 9 7.504024207987722 1103 10-11 6.629478430346726 1103 12-13 6.174479076314853 1103 14-15 7.119701691549306 1103 16-17 7.783841321411071 1103 18-19 8.60603233159378 1103 20-21 8.998467132368432 1103 22-23 8.639319573086446 1103 24-25 8.875389658807158 1103 26-27 8.696618642829076 1103 28-29 8.575358534200426 1103 30-31 8.343515560303768 1103 32-33 8.0099433779212 1103 34-35 10.574782836078974 1103 36-37 -3.247901365531387 1103 38-39 -1.8605767210661313 1103 40-41 7.349984827287237 1103 42-43 9.891667975830927 1103 44-45 1.0824476099489893 1103 46-47 0.1943123167972196 1103 48-49 8.046736081559175 1103 50-51 -5.392847538954117 1103 52-53 4.468602486530994 1103 54-55 7.662488040442927 1103 56-57 8.42956486079769 1103 58-59 8.584916134401631 1103 60-61 8.110804201386522 1103 62-63 8.329991834447473 1103 64-65 8.40423485639693 1103 66-67 8.266038482294793 1103 68-69 7.7708254135161035 1103 70-71 7.991461462300361 1103 72-73 7.728673808275932 1103 74-75 7.515005819950716 1103 76-77 6.988477411861803 1103 78-79 6.8819073390002625 1103 80-81 7.080689443621932 1103 82-83 6.773715845959741 1103 84-85 6.760713939822695 1103 86-87 6.832996439592202 1103 88-89 6.903000030879314 1103 90-91 6.68381448831574 1103 92-93 6.922520610256816 1103 94-95 6.6265894962394185 1103 96-97 6.6359854093852615 1103 98-99 6.055144878733145 1103 100-101 6.114455808485745 1104 1 6.441329247720745 1104 2 7.635866940319506 1104 3 7.126961214114051 1104 4 9.231255467882534 1104 5 8.959298789957906 1104 6 7.929923635290329 1104 7 7.769981259956932 1104 8 7.648956742324803 1104 9 7.7945847006054585 1104 10-11 7.070359637633363 1104 12-13 6.647776956099335 1104 14-15 6.905046780533045 1104 16-17 7.444907726841063 1104 18-19 7.9960213689832855 1104 20-21 8.325330193035981 1104 22-23 8.070868566625506 1104 24-25 8.01673502768734 1104 26-27 7.656504818277494 1104 28-29 7.422814975317209 1104 30-31 7.367619308583574 1104 32-33 7.315221758253809 1104 34-35 9.571741294782587 1104 36-37 -3.448988296700186 1104 38-39 -2.731493148654593 1104 40-41 5.479376285780926 1104 42-43 8.03870223981992 1104 44-45 0.004885974420801631 1104 46-47 -0.7752396156544386 1104 48-49 5.9366947968345265 1104 50-51 -5.975258380276161 1104 52-53 3.5223775897428027 1104 54-55 6.479120886289962 1104 56-57 6.94482154915633 1104 58-59 7.448489945424885 1104 60-61 7.097821671589212 1104 62-63 6.80370722641911 1104 64-65 6.890107083672834 1104 66-67 6.333041024687439 1104 68-69 6.377342335588267 1104 70-71 6.151154042712275 1104 72-73 6.273186671849739 1104 74-75 6.5290053068072424 1104 76-77 6.635266766467266 1104 78-79 6.617559147830997 1104 80-81 6.668220057294874 1104 82-83 6.475015031927599 1104 84-85 6.273654485154255 1104 86-87 5.929882591715918 1104 88-89 5.348732776430127 1104 90-91 4.973973796038546 1104 92-93 4.82044237920231 1104 94-95 4.49320718102938 1104 96-97 4.6203461958942516 1104 98-99 4.258067464043247 1104 100-101 3.225756627182827 1105 1 5.83842247707204 1105 2 6.913664411665366 1105 3 6.106353864474443 1105 4 8.39523987057661 1105 5 8.049597738321367 1105 6 7.179905911079054 1105 7 7.262643436490077 1105 8 6.537766856941371 1105 9 6.3794836726012 1105 10-11 5.862962733462272 1105 12-13 5.592335623238647 1105 14-15 6.065793560634663 1105 16-17 6.70726150209806 1105 18-19 7.365334851199989 1105 20-21 7.491902330575861 1105 22-23 6.990956006067783 1105 24-25 7.072448131787539 1105 26-27 7.455914011235077 1105 28-29 7.368543440400515 1105 30-31 7.131840034094619 1105 32-33 6.6936100366420845 1105 34-35 8.964060803231128 1105 36-37 -3.8627186523660253 1105 38-39 -2.8793367029497574 1105 40-41 5.2219616573985554 1105 42-43 7.898137428287363 1105 44-45 -3.1860155971259303 1105 46-47 -5.029286643895016 1105 48-49 4.131909259790923 1105 50-51 -10.056624326158236 1105 52-53 -0.4968000068541478 1105 54-55 4.055122304226863 1105 56-57 5.255077959412741 1105 58-59 5.716031006514335 1105 60-61 5.843892804757118 1105 62-63 5.615889667633809 1105 64-65 5.10116699150694 1105 66-67 4.7776115079676025 1105 68-69 4.636659362647233 1105 70-71 4.902152506613966 1105 72-73 4.607689803127066 1105 74-75 4.811127485703619 1105 76-77 4.544223401230353 1105 78-79 4.388243302386117 1105 80-81 4.461343063321102 1105 82-83 4.917328632305717 1105 84-85 4.539777609341895 1105 86-87 4.15480283276519 1105 88-89 3.6367393934690018 1105 90-91 3.6719768682351663 1105 92-93 3.282299876543677 1105 94-95 2.8876535948306348 1105 96-97 2.5374323355610375 1105 98-99 2.292972344109417 1105 100-101 2.296582575428131 1106 1 5.506849572364807 1106 2 6.904184339498727 1106 3 6.41726086791876 1106 4 7.750988599459667 1106 5 7.98950260956952 1106 6 6.91521835982434 1106 7 6.977519605097587 1106 8 5.971849192516242 1106 9 5.875498105929065 1106 10-11 5.683891058868202 1106 12-13 5.722809626846981 1106 14-15 5.684458477807045 1106 16-17 5.920923959044099 1106 18-19 6.805797373752071 1106 20-21 7.139155077828605 1106 22-23 7.02810542381421 1106 24-25 6.8358399638360865 1106 26-27 6.79298142143681 1106 28-29 6.396540816159085 1106 30-31 5.968070967340477 1106 32-33 6.365825880499717 1106 34-35 8.95467914011812 1106 36-37 -0.9398221458874296 1106 38-39 0.28344827326805344 1106 40-41 5.713170448607347 1106 42-43 6.573420354316561 1106 44-45 -3.6225253560237487 1106 46-47 -5.021430321113321 1106 48-49 3.947933534024152 1106 50-51 -10.010421275477572 1106 52-53 -0.5132507203197854 1106 54-55 4.150693896813383 1106 56-57 5.139988571189022 1106 58-59 5.520147785257976 1106 60-61 5.3537173086114755 1106 62-63 5.607295276949866 1106 64-65 5.5521264797798615 1106 66-67 5.6323761463143285 1106 68-69 5.381829118264747 1106 70-71 4.694050161198188 1106 72-73 4.821204646492657 1106 74-75 5.1408470199007645 1106 76-77 4.736908328243638 1106 78-79 4.573071906617708 1106 80-81 4.159374882248443 1106 82-83 4.712326172079374 1106 84-85 4.505990500927924 1106 86-87 4.605073457662678 1106 88-89 4.226094814167707 1106 90-91 3.7879683394505186 1106 92-93 3.6070989580591775 1106 94-95 3.476172538573458 1106 96-97 2.9248031736781446 1106 98-99 2.538552137450406 1106 100-101 2.0334531675226035 1107 1 5.5444733881862405 1107 2 6.889330939230625 1107 3 6.24054498980481 1107 4 7.214268942770243 1107 5 7.710612251234615 1107 6 6.609738525721795 1107 7 6.37072986482967 1107 8 6.231725219254162 1107 9 6.1825253566555425 1107 10-11 5.52063185758708 1107 12-13 5.204323845986366 1107 14-15 5.610688539073951 1107 16-17 6.688190699482956 1107 18-19 6.9876165779121 1107 20-21 7.3022051079672465 1107 22-23 7.1064681310735835 1107 24-25 7.115333094925667 1107 26-27 7.549209323517726 1107 28-29 7.524659265503686 1107 30-31 6.969415960278138 1107 32-33 6.6937027709120365 1107 34-35 9.139980031682004 1107 36-37 -0.6084343290943615 1107 38-39 0.007202917767077821 1107 40-41 5.9518684594572555 1107 42-43 7.5223758480954075 1107 44-45 -3.7289237438315457 1107 46-47 -4.992893079653353 1107 48-49 4.212242639491393 1107 50-51 -9.955175353190276 1107 52-53 -0.5105269971634172 1107 54-55 4.333925568473166 1107 56-57 5.545454460548733 1107 58-59 5.922629049821239 1107 60-61 6.252428994305969 1107 62-63 5.935271130053245 1107 64-65 5.072535285660042 1107 66-67 5.686940575524517 1107 68-69 5.687700304827413 1107 70-71 5.941272458392145 1107 72-73 6.067851160756778 1107 74-75 6.0633322429716685 1107 76-77 5.812760981793248 1107 78-79 5.923582014307106 1107 80-81 5.679646489852381 1107 82-83 5.230125961558739 1107 84-85 5.1521831363043855 1107 86-87 4.853395590218705 1107 88-89 4.1661616589672175 1107 90-91 4.3040745174214265 1107 92-93 3.7521287818156193 1107 94-95 3.8049670830301974 1107 96-97 3.8079057440091315 1107 98-99 3.2899376151253215 1107 100-101 2.748323661346431 1108 1 6.1635032786234305 1108 2 6.5687710697132005 1108 3 6.820057807590153 1108 4 8.579629010848109 1108 5 8.374646219252199 1108 6 7.62589815413012 1108 7 7.668978278119038 1108 8 6.4828864432374225 1108 9 5.0652949201771555 1108 10-11 5.707549029519146 1108 12-13 5.342416424790038 1108 14-15 5.684975848934602 1108 16-17 6.372306750966839 1108 18-19 6.906543642408778 1108 20-21 7.617096254299199 1108 22-23 7.597856458556471 1108 24-25 6.893973665077777 1108 26-27 7.527084017699192 1108 28-29 7.655195024110924 1108 30-31 7.339904028923318 1108 32-33 7.683429041166971 1108 34-35 10.212800299029446 1108 36-37 -3.407870559282639 1108 38-39 -2.4607038651992745 1108 40-41 5.2898511208762535 1108 42-43 8.065283517492276 1108 44-45 -2.851109326925542 1108 46-47 -4.424713276086354 1108 48-49 5.0937224466041116 1108 50-51 -9.829200279616543 1108 52-53 0.6705884870049346 1108 54-55 6.000468134866814 1108 56-57 7.03088597492664 1108 58-59 6.865077548207935 1108 60-61 7.190870826735143 1108 62-63 7.31642295787298 1108 64-65 7.141082957893492 1108 66-67 7.037226892582986 1108 68-69 6.714341974153374 1108 70-71 6.7784130108156475 1108 72-73 6.801403442428942 1108 74-75 6.512194066181962 1108 76-77 6.310092825923308 1108 78-79 6.659833476917468 1108 80-81 6.463056056210569 1108 82-83 6.339662181109853 1108 84-85 6.2986657799947725 1108 86-87 6.721011623973981 1108 88-89 6.051364474270555 1108 90-91 6.292985271596514 1108 92-93 5.987648939245682 1108 94-95 5.7585003950892 1108 96-97 5.4691873452572235 1108 98-99 5.371786177335014 1108 100-101 5.067881864374479 1109 1 4.731994391882893 1109 2 6.262011197622769 1109 3 6.171232790857797 1109 4 8.00182355238153 1109 5 7.467510798712304 1109 6 4.993340706372258 1109 7 5.205622139203292 1109 8 3.063406261784312 1109 9 2.8591151031175883 1109 10-11 2.2436444411351246 1109 12-13 2.5869505216146074 1109 14-15 3.276687754935942 1109 16-17 2.048740156187339 1109 18-19 3.0152667776805906 1109 20-21 3.529483775679431 1109 22-23 5.04361573571866 1109 24-25 5.564532153960059 1109 26-27 4.240244945787246 1109 28-29 4.502568044115385 1109 30-31 3.924337359777784 1109 32-33 3.9598382774278456 1109 34-35 5.796424899312036 1109 36-37 -4.883106671426612 1109 38-39 -4.128947711410323 1109 40-41 2.348600284258424 1109 42-43 3.245463111453759 1109 44-45 -4.928561546751526 1109 46-47 -7.052604255486964 1109 48-49 0.9900562013449452 1109 50-51 -10.353638816093081 1109 52-53 -2.292209906688825 1109 54-55 1.4201714144087205 1109 56-57 3.282800900188775 1109 58-59 3.7729380375364094 1109 60-61 4.093841749617567 1109 62-63 3.973521195398078 1109 64-65 3.539536144477861 1109 66-67 3.9079601416348204 1109 68-69 3.959833533609011 1109 70-71 3.803511545804863 1109 72-73 3.5858163226252664 1109 74-75 3.8260744856063695 1109 76-77 3.8790673183751547 1109 78-79 3.7952354253074407 1109 80-81 3.5857693008004397 1109 82-83 3.4800559492365704 1109 84-85 3.501777998333436 1109 86-87 3.9300824204341556 1109 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2313 84-85 -11.734947665383379 2313 86-87 -11.15398837602602 2313 88-89 -10.65556829883869 2313 90-91 -10.08516598890769 2313 92-93 -9.444073749829949 2313 94-95 -8.754104646927608 2313 96-97 -8.057073158944457 2313 98-99 -7.230104578967506 2313 100-101 -6.196824017978463 2314 1 -21.126412687763125 2314 2 -19.903918005917053 2314 3 -20.335404377283798 2314 4 -22.330034854698113 2314 5 -22.24930336057973 2314 6 -23.04426991309677 2314 7 -23.14614777230113 2314 8 -23.99190347272896 2314 9 -25.218318525200996 2314 10-11 -25.49308122258169 2314 12-13 -25.429642398739375 2314 14-15 -25.708931714090607 2314 16-17 -24.742189047352486 2314 18-19 -24.05774207187693 2314 20-21 -23.503702065028534 2314 22-23 -23.44310992799815 2314 24-25 -23.0682112088718 2314 26-27 -22.7166134612924 2314 28-29 -22.65783018597311 2314 30-31 -22.604423702169118 2314 32-33 -22.25144490841286 2314 34-35 -19.278796339626016 2314 36-37 -17.624257113904488 2314 38-39 -16.604611428224484 2314 40-41 -17.858258122821223 2314 42-43 -17.7655988354489 2314 44-45 -15.809092520202853 2314 46-47 -16.516099830708203 2314 48-49 -15.995563267681604 2314 50-51 -13.973107842641753 2314 52-53 -16.655041765095906 2314 54-55 -18.27894362983907 2314 56-57 -18.421845117510337 2314 58-59 -18.024628334145007 2314 60-61 -17.648414887550572 2314 62-63 -17.10059384884971 2314 64-65 -16.613118722778776 2314 66-67 -16.194915964559872 2314 68-69 -15.721582395594528 2314 70-71 -15.069276064814606 2314 72-73 -14.596705801268538 2314 74-75 -14.1075538329777 2314 76-77 -13.572260115253163 2314 78-79 -13.09856988442707 2314 80-81 -12.618876716898676 2314 82-83 -12.200253785276702 2314 84-85 -11.734947665383379 2314 86-87 -11.15398837602602 2314 88-89 -10.65556829883869 2314 90-91 -10.08516598890769 2314 92-93 -9.444073749829949 2314 94-95 -8.754104646927608 2314 96-97 -8.057073158944457 2314 98-99 -7.230104578967506 2314 100-101 -6.196824017978463 2315 1 -21.126412687763125 2315 2 -19.903918005917053 2315 3 -20.335404377283798 2315 4 -17.049333100312147 2315 5 -22.24930336057973 2315 6 -23.04426991309677 2315 7 -23.14614777230113 2315 8 -23.99190347272896 2315 9 -20.343318525200996 2315 10-11 -18.850537362932567 2315 12-13 -15.091923100493762 2315 14-15 -14.975379082511658 2315 16-17 -20.417627643843716 2315 18-19 -24.05774207187693 2315 20-21 -23.503702065028534 2315 22-23 -23.44310992799815 2315 24-25 -23.0682112088718 2315 26-27 -22.7166134612924 2315 28-29 -22.65783018597311 2315 30-31 -22.604423702169118 2315 32-33 -22.25144490841286 2315 34-35 -19.278796339626016 2315 36-37 -17.624257113904488 2315 38-39 -16.604611428224484 2315 40-41 -17.858258122821223 2315 42-43 -17.7655988354489 2315 44-45 -15.809092520202853 2315 46-47 -16.516099830708203 2315 48-49 -15.995563267681604 2315 50-51 -13.973107842641753 2315 52-53 -16.655041765095906 2315 54-55 -18.27894362983907 2315 56-57 -18.421845117510337 2315 58-59 -18.024628334145007 2315 60-61 -17.648414887550572 2315 62-63 -17.10059384884971 2315 64-65 -16.613118722778776 2315 66-67 -16.194915964559872 2315 68-69 -15.721582395594528 2315 70-71 -15.069276064814606 2315 72-73 -14.596705801268538 2315 74-75 -14.1075538329777 2315 76-77 -13.572260115253163 2315 78-79 -13.09856988442707 2315 80-81 -12.618876716898676 2315 82-83 -12.200253785276702 2315 84-85 -11.734947665383379 2315 86-87 -11.15398837602602 2315 88-89 -10.65556829883869 2315 90-91 -10.08516598890769 2315 92-93 -9.444073749829949 2315 94-95 -8.754104646927608 2315 96-97 -8.057073158944457 2315 98-99 -7.230104578967506 2315 100-101 -6.196824017978463 2316 1 -21.126412687763125 2316 2 -19.903918005917053 2316 3 -20.335404377283798 2316 4 -22.330034854698113 2316 5 -22.24930336057973 2316 6 -23.04426991309677 2316 7 -23.14614777230113 2316 8 -23.99190347272896 2316 9 -25.218318525200996 2316 10-11 -25.49308122258169 2316 12-13 -25.429642398739375 2316 14-15 -25.708931714090607 2316 16-17 -24.742189047352486 2316 18-19 -24.05774207187693 2316 20-21 -23.503702065028534 2316 22-23 -23.44310992799815 2316 24-25 -23.0682112088718 2316 26-27 -22.7166134612924 2316 28-29 -22.65783018597311 2316 30-31 -22.604423702169118 2316 32-33 -22.25144490841286 2316 34-35 -19.278796339626016 2316 36-37 -17.624257113904488 2316 38-39 -16.604611428224484 2316 40-41 -17.858258122821223 2316 42-43 -17.7655988354489 2316 44-45 -15.809092520202853 2316 46-47 -16.516099830708203 2316 48-49 -15.995563267681604 2316 50-51 -13.973107842641753 2316 52-53 -16.655041765095906 2316 54-55 -18.27894362983907 2316 56-57 -18.421845117510337 2316 58-59 -18.024628334145007 2316 60-61 -17.648414887550572 2316 62-63 -17.10059384884971 2316 64-65 -16.613118722778776 2316 66-67 -16.194915964559872 2316 68-69 -15.721582395594528 2316 70-71 -15.069276064814606 2316 72-73 -14.596705801268538 2316 74-75 -14.1075538329777 2316 76-77 -13.572260115253163 2316 78-79 -13.09856988442707 2316 80-81 -12.618876716898676 2316 82-83 -12.200253785276702 2316 84-85 -11.734947665383379 2316 86-87 -11.15398837602602 2316 88-89 -10.65556829883869 2316 90-91 -10.08516598890769 2316 92-93 -9.444073749829949 2316 94-95 -8.754104646927608 2316 96-97 -8.057073158944457 2316 98-99 -7.230104578967506 2316 100-101 -6.196824017978463 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 68932.0 3 3866.0 4 8787.0 5 5057.0 6 4384.0 7 5602.0 8 7315.0 9 8859.0 10 9960.0 11 9471.0 12 8148.0 13 7346.0 14 6722.0 15 7008.0 16 7763.0 17 8435.0 18 8640.0 19 9027.0 20 9838.0 21 10449.0 22 11140.0 23 11743.0 24 12747.0 25 13350.0 26 14414.0 27 15421.0 28 16046.0 29 17201.0 30 17067.0 31 17257.0 32 16607.0 33 15820.0 34 13933.0 35 10993.0 36 6500.0 37 1870.0 38 166.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.05053653857683 14.959044312800081 11.42481102615387 26.56560812246922 2 18.21403491097435 17.703200419571715 35.88345625164821 28.199308417805725 3 21.814703855296287 20.899616017553484 25.966496476644586 31.31918365050565 4 15.156703528678007 13.826852495981962 31.51072276728956 39.505721208050474 5 16.78216709361377 34.95333543061735 31.043998022560782 17.220499453208095 6 37.85984095703728 30.346853745514586 15.494598042676436 16.298707254771706 7 32.66968449931413 29.155006858710564 17.938271604938272 20.237037037037037 8 29.873301583597623 31.664262455053617 17.686335239024597 20.776100722324166 9 28.84380517863122 13.909170402453213 16.938590173175086 40.30843424574048 10-11 29.04804714813705 23.03391269603436 24.374937568674458 23.54310258715413 12-13 28.99944059534217 20.661405195440487 24.497928265433877 25.841225943783463 14-15 25.873735445094344 21.680082574946972 22.407780596651882 30.038401383306805 16-17 26.50882349064934 24.46037666222966 22.82536209918684 26.205437747934155 18-19 26.438833955567436 23.748109085800102 24.301776947829183 25.51128001080328 20-21 27.203915573866013 22.912413712734526 24.324542943124097 25.559127770275364 22-23 27.11177991300518 22.710093568300575 24.11823691563323 26.05988960306102 24-25 26.743607381787054 22.831183046207162 24.048155035724772 26.377054536281015 26-27 26.495844411553943 23.24745340450183 24.09665342861161 26.160048755332614 28-29 26.55056682777496 23.32773073201069 24.03609790880895 26.085604531405394 30-31 26.745826507263843 23.07369447189916 23.990982818613844 26.189496202223157 32-33 26.577359484917768 23.25881604548225 23.959228867175753 26.20459560242423 34-35 26.937210808876678 23.10888663491546 23.960138335615362 25.993764220592503 36-37 27.17889767641469 23.421004230274047 23.621482435166453 25.77861565814481 38-39 27.05498374905575 23.361394659517362 23.613749797525497 25.969871793901394 40-41 27.150348181780647 23.107139755368664 23.684618922124315 26.057893140726378 42-43 26.974305848066393 23.235021231543357 23.535564258148245 26.255108662242005 44-45 27.050121352201543 23.401942244269648 23.502130134629382 26.045806268899423 46-47 26.653377926421406 23.246956521739133 23.60334448160535 26.496321070234114 48-49 26.83942241141156 23.54897353560618 23.333313529843362 26.278290523138892 50-51 27.03638873092598 23.63416382333428 23.35782745711307 25.97161998862667 52-53 26.529914395024417 23.349417967707105 23.552481544756183 26.568186092512292 54-55 26.01406201689423 23.133797962573567 23.899137888341752 26.953002132190456 56-57 26.173936427974247 23.350791055823887 23.506020118538558 26.96925239766331 58-59 26.369070548651873 23.39427669381147 23.512805109652255 26.7238476478844 60-61 26.290248128499655 23.27577786846313 23.67248911218298 26.76148489085423 62-63 26.078036985722463 23.31681261601502 23.593911317716664 27.011239080545852 64-65 26.058562704376637 23.229773480304047 23.50439612794248 27.207267687376834 66-67 26.119885802801257 23.43460430192379 23.318361225029328 27.127148670245628 68-69 26.017402009536966 23.347158531930564 23.508303183110858 27.12713627542161 70-71 25.83882982594256 23.183180401833855 23.663475723916193 27.314514048307387 72-73 25.95535689650415 23.376205323064024 23.35281821251526 27.315619567916567 74-75 25.927849624257888 23.448148903017113 23.305084188587696 27.318917284137306 76-77 25.922198674764886 23.089531642389108 23.425905097169437 27.56236458567657 78-79 25.90161681070772 23.091461553000016 23.398884238045078 27.608037398247188 80-81 25.984855391583654 23.26537946744147 23.363683329435535 27.38608181153934 82-83 26.107766843930154 23.26089442685672 23.33744002412031 27.29389870509282 84-85 26.042806927331064 23.245280862838896 23.108546971922156 27.60336523790788 86-87 25.73081928465842 23.39830804853537 23.38127672520155 27.489595941604662 88-89 25.80640900673868 23.140260090183617 23.309717588446777 27.743613314630934 90-91 25.962549914395765 23.11391335284742 23.162848405372323 27.760688327384493 92-93 26.058921214159593 22.833284284873457 23.31965231648869 27.788142184478264 94-95 25.91271536848471 22.814433057460082 23.442214523773526 27.83063705028168 96-97 25.731644456771164 22.82050409877887 23.3544050425577 28.09344640189227 98-99 25.413559959662884 22.84246056419715 23.434193545016118 28.309785931123848 100-101 26.099927672047013 22.680502711971258 23.012060326482885 28.207509289498844 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4567.0 1 5151.5 2 3871.5 3 1853.5 4 2286.0 5 3251.5 6 3703.0 7 3729.0 8 3492.5 9 3226.0 10 2978.0 11 2724.5 12 2500.0 13 2207.5 14 1918.0 15 1602.0 16 1296.5 17 1122.5 18 1009.5 19 892.5 20 820.0 21 817.5 22 844.5 23 938.0 24 1082.5 25 1229.5 26 1408.5 27 1551.5 28 1684.5 29 1813.0 30 1922.0 31 2071.0 32 2287.5 33 2410.5 34 2566.0 35 2818.5 36 2971.0 37 3186.5 38 3470.5 39 3770.0 40 4077.5 41 4453.0 42 5001.0 43 5581.0 44 6321.5 45 7153.0 46 8280.5 47 9839.5 48 11465.5 49 13286.0 50 15265.0 51 17294.5 52 19372.0 53 21184.5 54 21980.5 55 21713.0 56 20764.5 57 19238.0 58 17737.0 59 16132.0 60 13986.0 61 11881.5 62 9933.0 63 8008.0 64 6328.5 65 4902.5 66 3759.5 67 2941.5 68 2296.0 69 1731.5 70 1342.0 71 1058.0 72 778.0 73 568.0 74 436.5 75 338.0 76 245.5 77 155.5 78 105.0 79 80.0 80 47.5 81 26.5 82 17.0 83 4.5 84 5.0 85 4.5 86 3.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.233697760618483 2 21.126888937707708 3 16.920628391674416 4 22.059614009338937 5 21.99674679704407 6 15.917557480263433 7 14.813758804915327 8 11.865309918997117 9 2.981401588273512 10-11 6.414325310947308 12-13 3.493220156770729 14-15 7.281261831422388 16-17 11.335028488896576 18-19 14.333373842565544 20-21 14.767601650907016 22-23 12.717289184502414 24-25 15.233613626059277 26-27 12.75888904988712 28-29 12.817666387776184 30-31 13.693133217726217 32-33 12.194603235441216 34-35 19.009151970384636 36-37 23.76006693371599 38-39 22.809229561144793 40-41 17.926386934837783 42-43 17.883735387463016 44-45 23.25455845715915 46-47 12.651968047564072 48-49 21.32460515183951 50-51 21.093001406916795 52-53 11.150049312199979 54-55 12.69648925181006 56-57 12.225686280925293 58-59 13.243480740197155 60-61 12.66423767078147 62-63 12.62298836606012 64-65 13.664854657548972 66-67 12.040473397119793 68-69 13.054879103312564 70-71 11.683836348934062 72-73 11.062409146361414 74-75 11.133456107467877 76-77 11.171784073327942 78-79 11.587081605848287 80-81 11.56090640965117 82-83 10.08399433493968 84-85 10.010026034971933 86-87 10.119634669047363 88-89 11.527603146632515 90-91 9.019809014550605 92-93 11.056683322193294 94-95 11.119433680933707 96-97 10.68018116975082 98-99 10.542995096824855 100-101 10.65692731241499 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 427886.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 94.70491888144169 #Duplication Level Percentage of deduplicated Percentage of total 1 97.25690850254502 92.10707630393323 2 1.8608974006523535 3.524722747509337 3 0.43531631580917934 1.2367978912942916 4 0.17249271200165792 0.6534363319102758 5 0.08875284798531757 0.42026656344732133 6 0.05358669680658069 0.3044954264514981 7 0.03156884749776429 0.20928075990194595 8 0.0255917507616616 0.19389317439337883 9 0.017823743604837256 0.15191965730337548 >10 0.05483614885022543 0.8129588766432052 >50 0.0014833557098960929 0.1074680244929395 >100 4.944518503795554E-4 0.09552712565718806 >500 2.472259251897777E-4 0.18215711706199095 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 778 0.18182413072640843 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.3370710890283861E-4 7 0.0 0.0 0.0 0.0 2.3370710890283861E-4 8 0.0 0.0 0.0 0.0 2.3370710890283861E-4 9 0.0 0.0 0.0 0.0 2.3370710890283861E-4 10-11 0.0 0.0 0.0 0.0 2.3370710890283861E-4 12-13 0.0 0.0 0.0 0.0 7.011213267085158E-4 14-15 0.0 0.0 0.0 0.0 7.011213267085158E-4 16-17 0.0 0.0 0.0 0.0 9.348284356113545E-4 18-19 0.0 0.0 0.0 1.1685355445141931E-4 0.0010516819900627738 20-21 0.0 0.0 0.0 3.505606633542579E-4 0.0014022426534170316 22-23 2.3370710890283861E-4 0.0 0.0 4.6741421780567723E-4 0.0017528033167712896 24-25 2.3370710890283861E-4 0.0 0.0 0.0014022426534170316 0.001869656871222709 26-27 2.3370710890283861E-4 0.0 0.0 0.003155045970188321 0.001869656871222709 28-29 2.3370710890283861E-4 0.0 0.0 0.0066606526037309 0.001869656871222709 30-31 2.3370710890283861E-4 0.0 0.0 0.02021566492009554 0.0019865104256741282 32-33 2.3370710890283861E-4 0.0 0.0 0.050130174859658885 0.0021033639801255476 34-35 2.3370710890283861E-4 0.0 0.0 0.09137947958100989 0.0021033639801255476 36-37 2.3370710890283861E-4 0.0 0.0 0.14863772126220537 0.0021033639801255476 38-39 2.3370710890283861E-4 0.0 0.0 0.23137003781381021 0.0021033639801255476 40-41 2.3370710890283861E-4 0.0 0.0 0.309077651524004 0.0021033639801255476 42-43 2.3370710890283861E-4 0.0 0.0 0.38409763348181525 0.0021033639801255476 44-45 2.3370710890283861E-4 0.0 0.0 0.4782815983696592 0.002220217534576967 46-47 2.3370710890283861E-4 0.0 0.0 0.5820475547225196 0.0023370710890283863 48-49 2.3370710890283861E-4 0.0 0.0 0.6880337286099569 0.0023370710890283863 50-51 2.3370710890283861E-4 0.0 0.0 0.8162220778431638 0.0023370710890283863 52-53 2.3370710890283861E-4 0.0 0.0 0.9789990791939909 0.0025707781979312245 54-55 2.3370710890283861E-4 2.3370710890283861E-4 0.0 1.1444637122972008 0.0025707781979312245 56-57 2.3370710890283861E-4 2.3370710890283861E-4 0.0 1.324301332597935 0.0025707781979312245 58-59 2.3370710890283861E-4 2.3370710890283861E-4 0.0 1.515240040571554 0.0025707781979312245 60-61 2.3370710890283861E-4 2.3370710890283861E-4 0.0 1.7258101456930115 0.0025707781979312245 62-63 2.3370710890283861E-4 2.3370710890283861E-4 0.0 1.9317061086364125 0.0025707781979312245 64-65 2.3370710890283861E-4 2.3370710890283861E-4 0.0 2.1373683644709107 0.0025707781979312245 66-67 2.3370710890283861E-4 2.3370710890283861E-4 0.0 2.352612611770425 0.0025707781979312245 68-69 2.3370710890283861E-4 2.3370710890283861E-4 0.0 2.5879556704355835 0.0025707781979312245 70-71 2.3370710890283861E-4 2.3370710890283861E-4 0.0 2.8429301262485804 0.002687631752382644 72-73 2.3370710890283861E-4 2.3370710890283861E-4 0.0 3.1026955777940852 0.0029213388612854825 74-75 2.3370710890283861E-4 2.3370710890283861E-4 0.0 3.342011657310592 0.003038192415736902 76-77 2.3370710890283861E-4 2.3370710890283861E-4 0.0 3.6055164225985425 0.003038192415736902 78-79 2.3370710890283861E-4 2.3370710890283861E-4 0.0 3.9100367854989413 0.003038192415736902 80-81 2.3370710890283861E-4 2.3370710890283861E-4 0.0 4.224255993418808 0.003155045970188321 82-83 2.3370710890283861E-4 2.3370710890283861E-4 0.0 4.538358347784223 0.0032718995246397405 84-85 2.3370710890283861E-4 2.3370710890283861E-4 0.0 4.855265187456473 0.0032718995246397405 86-87 2.3370710890283861E-4 2.3370710890283861E-4 0.0 5.168666420495178 0.003505606633542579 88-89 2.3370710890283861E-4 2.3370710890283861E-4 0.0 5.502750732671787 0.003739313742445418 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 325 0.0 55.71708 1 GTATCAA 615 0.0 50.709084 1 ATACTAG 50 0.001218036 40.24011 1 GTGGTAT 105 2.8016075E-8 38.323917 1 TCAACGC 860 0.0 36.21767 4 ATCAACG 865 0.0 34.857628 3 TATCAAC 905 0.0 34.438263 2 CAACGCA 905 0.0 34.15342 5 TGGTATC 120 9.12205E-8 33.512505 2 AACGCAG 980 0.0 31.873365 6 GTACATG 1470 0.0 31.822538 1 CTAGTAC 80 3.2248851E-4 31.408176 3 TACATGG 1460 0.0 31.331898 2 GTACACG 85 4.5752747E-4 29.58832 1 CTATACC 85 4.6088413E-4 29.55142 4 ACATGGG 1610 0.0 29.028177 3 GTAAGGT 105 4.704124E-5 28.707094 4 CCTAGTA 75 0.008902628 26.810003 2 ACGCAGA 1190 0.0 26.267105 7 AGTACAT 980 0.0 25.134378 2 >>END_MODULE