FastQCFastQC Report
Sat 18 Jun 2016
SRR1930927_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930927_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427886
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT9810.22926667383368465No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT6740.15751859140051322RNA PCR Primer, Index 46 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5480.12807149567875556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5320.12433218193631014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5600.062.7750431
ATACCGA250.005037792556.9465686
TATACCG558.2316546E-751.769615
GTATAAA554.9547078E-543.1867641
AATATAG459.6627814E-442.1826485
GTATCAA8850.039.171532
GTATTAT550.002576373334.5494081
CTAACGC600.003952772431.6591843
TCTAACG600.003952772431.6591842
TCTATAC753.0795066E-431.659183
GTACATG19250.030.6009081
TGGTATC2051.8189894E-1230.1148342
TACATGG19300.029.52672
CGTACAC650.005846770529.2238623
GTGGTAT2151.8189894E-1228.724221
ACATGGG20050.028.1853563
CCTAGTA1354.223366E-728.1414972
TATTGGA856.409402E-427.9345722
TCAACGC12250.027.911774
CAACGCA12550.027.2254525
Produced by FastQC (version 0.11.5)