FastQCFastQC Report
Sat 18 Jun 2016
SRR1930926_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930926_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5098588
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN92920.18224653570753313No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33750.047.7292631
GTATCAA62950.041.0716361
TCAACGC71250.035.197384
ATCAACG70800.034.862053
CAACGCA74500.032.9396975
TATCAAC77750.032.401422
AACGCAG79400.031.0516286
GTACATG88050.025.9798531
ACGCAGA93850.025.5622067
CGCAGAG98300.024.7643898
TACATGG93600.024.5414072
ACATGGG94350.023.6994953
GCAGAGT111900.020.9374669
CATGGGG70350.019.5811124
GAGTACA70300.017.8859421
CAGAGTA107900.017.86442410-11
AGAGTAC101200.017.78170210-11
GAGTACT65700.017.72455612-13
GTACTTT76700.015.54319814-15
ATGGGGG48050.015.51297955
Produced by FastQC (version 0.11.5)