##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930926_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5098588 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.960666561016502 30.0 10.0 31.0 2.0 33.0 2 21.767957128522642 30.0 10.0 31.0 2.0 33.0 3 22.24314829909771 30.0 10.0 31.0 2.0 33.0 4 24.164370606136444 33.0 2.0 35.0 2.0 37.0 5 24.142128958056624 32.0 10.0 35.0 2.0 37.0 6 24.947002189625834 32.0 11.0 35.0 2.0 37.0 7 25.045149951319857 32.0 11.0 35.0 2.0 37.0 8 25.899200916018316 33.0 17.0 35.0 2.0 37.0 9 27.110809894817937 34.0 17.0 37.0 2.0 39.0 10-11 27.40821301897702 34.0 18.5 37.0 2.0 39.0 12-13 27.389349364961436 34.0 18.5 37.0 2.0 39.0 14-15 27.62610157949613 34.0 17.5 38.0 2.0 40.0 16-17 26.712407435156557 33.0 14.0 38.0 2.0 40.0 18-19 26.035333115756757 33.0 10.0 38.0 2.0 40.0 20-21 25.501989864644877 32.0 10.0 38.0 2.0 40.0 22-23 25.440333382497272 32.0 10.0 38.0 2.0 40.0 24-25 25.16684795868974 32.0 10.0 38.0 2.0 40.0 26-27 24.86022120241918 32.0 8.5 38.0 2.0 40.0 28-29 24.813450704391098 32.0 8.5 38.0 2.0 40.0 30-31 24.75475896463884 31.5 8.0 38.0 2.0 40.0 32-33 24.488835928692414 31.0 5.0 38.0 2.0 40.0 34-35 21.552485903940465 26.5 2.0 36.0 2.0 39.0 36-37 19.88775951302596 19.0 2.0 35.0 2.0 38.5 38-39 18.966665574861118 19.0 2.0 34.0 2.0 38.0 40-41 20.381945060083304 24.5 2.0 35.0 2.0 38.0 42-43 20.315489504152914 24.5 2.0 35.0 2.0 38.0 44-45 18.313361758196585 19.0 2.0 34.0 2.0 38.0 46-47 19.196279048238452 19.0 2.0 33.0 2.0 38.0 48-49 18.664808276330625 19.5 2.0 34.0 2.0 38.0 50-51 16.62044560180191 11.5 2.0 32.0 2.0 37.5 52-53 19.593639945020072 23.0 2.0 33.5 2.0 38.0 54-55 21.31880630480439 27.5 2.0 35.5 2.0 38.5 56-57 21.560377402527916 28.0 2.0 36.0 2.0 39.0 58-59 21.185475272761792 28.0 2.0 36.0 2.0 39.0 60-61 20.888718896290506 27.5 2.0 36.0 2.0 39.0 62-63 20.42115042439201 26.5 2.0 35.0 2.0 39.0 64-65 19.925509964719645 26.0 2.0 34.5 2.0 38.0 66-67 19.5114301253602 25.5 2.0 34.0 2.0 38.0 68-69 19.069414512410102 24.0 2.0 34.0 2.0 38.0 70-71 18.476119466801396 22.0 2.0 34.0 2.0 37.0 72-73 18.009280902869577 21.5 2.0 33.0 2.0 36.0 74-75 17.46708255305194 19.0 2.0 32.5 2.0 36.0 76-77 16.907117127330153 15.5 2.0 32.0 2.0 35.5 78-79 16.415836502184526 12.0 2.0 32.0 2.0 35.0 80-81 15.910342726260684 7.5 2.0 31.0 2.0 35.0 82-83 15.432309494314897 4.5 2.0 31.0 2.0 34.0 84-85 14.904267122583743 2.0 2.0 31.0 2.0 34.0 86-87 14.332935314640054 2.0 2.0 30.0 2.0 34.0 88-89 13.769991613364327 2.0 2.0 30.0 2.0 34.0 90-91 13.185775944241819 2.0 2.0 29.0 2.0 34.0 92-93 12.54486781438312 2.0 2.0 29.0 2.0 34.0 94-95 11.871844910787065 2.0 2.0 28.0 2.0 34.0 96-97 11.123014156076152 2.0 2.0 26.5 2.0 33.5 98-99 10.298268265645312 2.0 2.0 25.0 2.0 33.0 100-101 9.189291329285677 2.0 2.0 20.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.613898035934454 1101 2 7.761310155050104 1101 3 7.3503628515655635 1101 4 9.100147472733294 1101 5 8.944769609984046 1101 6 8.321951791491013 1101 7 8.11594919186306 1101 8 7.196251689504404 1101 9 7.386774434893351 1101 10-11 6.72566776635249 1101 12-13 6.49182088183181 1101 14-15 6.935457705796878 1101 16-17 7.758977021890061 1101 18-19 8.249850345437263 1101 20-21 8.740113405881576 1101 22-23 8.651176176039396 1101 24-25 8.651723576707486 1101 26-27 8.59937979722854 1101 28-29 8.511138910447102 1101 30-31 8.242029153949161 1101 32-33 8.165273726746118 1101 34-35 10.724070511656308 1101 36-37 4.912331663788702 1101 38-39 8.084668508281133 1101 40-41 9.585112063077265 1101 42-43 10.015954851668592 1101 44-45 4.2598118520252015 1101 46-47 5.848673416287333 1101 48-49 8.93245692644302 1101 50-51 -4.072215220215231 1101 52-53 5.575298273516854 1101 54-55 8.203792223681145 1101 56-57 8.640024656918118 1101 58-59 8.943371212936949 1101 60-61 8.652470940072114 1101 62-63 8.719438080669402 1101 64-65 8.586943744976264 1101 66-67 8.5546110816375 1101 68-69 8.544735653384645 1101 70-71 8.640275465382494 1101 72-73 8.397528216743812 1101 74-75 8.497700618890857 1101 76-77 8.349992753812952 1101 78-79 8.292966241357899 1101 80-81 8.193872400609946 1101 82-83 7.871501431035357 1101 84-85 7.834650017019962 1101 86-87 7.705720156722515 1101 88-89 7.585770739412647 1101 90-91 7.521243845173876 1101 92-93 7.383078141097991 1101 94-95 7.076397488288082 1101 96-97 6.869408352382896 1101 98-99 6.522126618537262 1101 100-101 6.0163026742337955 1102 1 6.412926679581492 1102 2 7.468814827033626 1102 3 7.344511539054395 1102 4 9.012657603968282 1102 5 9.158640262829213 1102 6 8.32038036112196 1102 7 8.150714653773164 1102 8 7.097163532918106 1102 9 7.047112440640408 1102 10-11 6.599598441118555 1102 12-13 6.268582930257825 1102 14-15 6.569678964709297 1102 16-17 7.5076041830857605 1102 18-19 7.867995266300699 1102 20-21 8.248361126515032 1102 22-23 7.978375037178882 1102 24-25 8.087201241851464 1102 26-27 8.120765616274358 1102 28-29 7.888307406113583 1102 30-31 7.806479961156917 1102 32-33 7.8692075013796945 1102 34-35 10.103933222129488 1102 36-37 0.7186521750869623 1102 38-39 3.1200070585014075 1102 40-41 8.023147058427863 1102 42-43 9.100801121470479 1102 44-45 0.803924205001298 1102 46-47 1.2813709205188992 1102 48-49 7.29342070937518 1102 50-51 -5.258113319511658 1102 52-53 4.2526274331470475 1102 54-55 7.2775042178372935 1102 56-57 7.666543466975529 1102 58-59 7.997548375165124 1102 60-61 7.782958705799242 1102 62-63 7.685145120683909 1102 64-65 7.727166913658742 1102 66-67 7.786489097212499 1102 68-69 7.751567739618878 1102 70-71 7.688904102705578 1102 72-73 7.641355322217713 1102 74-75 7.318135106098719 1102 76-77 7.2697733165869245 1102 78-79 7.32373450355426 1102 80-81 7.218372203147657 1102 82-83 7.02386415441457 1102 84-85 7.0456292952565835 1102 86-87 6.973111579083378 1102 88-89 6.911858149223685 1102 90-91 6.87033732549831 1102 92-93 6.830386261476422 1102 94-95 6.478887033595674 1102 96-97 6.26339096747405 1102 98-99 5.984851982051781 1102 100-101 5.641261516091671 1103 1 6.414026108144249 1103 2 7.5053598387836296 1103 3 7.21567017929177 1103 4 8.867156280986084 1103 5 8.92453960614436 1103 6 8.150132251367555 1103 7 7.9600812665593 1103 8 6.999308383026499 1103 9 7.1028127105490135 1103 10-11 6.412116859788032 1103 12-13 6.317616669465902 1103 14-15 6.701410000278045 1103 16-17 7.353266957958709 1103 18-19 7.84558504189965 1103 20-21 8.172130126993121 1103 22-23 8.160509987054805 1103 24-25 8.114054239051189 1103 26-27 8.130661896240664 1103 28-29 8.057414981261758 1103 30-31 7.8494704795736965 1103 32-33 7.89389895309338 1103 34-35 10.075515823775248 1103 36-37 -3.419720727556218 1103 38-39 -1.9452240428370047 1103 40-41 6.54566305184078 1103 42-43 8.881038410849431 1103 44-45 0.5696557249889551 1103 46-47 0.3337474506123179 1103 48-49 7.3122226927101615 1103 50-51 -5.488297597904552 1103 52-53 4.239667804340943 1103 54-55 7.211069578976019 1103 56-57 7.891771147026326 1103 58-59 7.947888243094567 1103 60-61 7.9628370129385395 1103 62-63 8.07213614881562 1103 64-65 8.00335170050177 1103 66-67 7.8665224848058095 1103 68-69 7.764863252230214 1103 70-71 7.846767435861839 1103 72-73 7.766898001041646 1103 74-75 7.743369616076738 1103 76-77 7.522187427090088 1103 78-79 7.446951924169003 1103 80-81 7.273396911041791 1103 82-83 7.007307588704345 1103 84-85 6.909053438163957 1103 86-87 6.673831519814788 1103 88-89 6.555918468231875 1103 90-91 6.476853834950168 1103 92-93 6.284670096177008 1103 94-95 6.039062723987483 1103 96-97 5.908182499755574 1103 98-99 5.660700157828812 1103 100-101 5.159001698703687 1104 1 6.259362508546769 1104 2 7.515992996764965 1104 3 6.937040033837789 1104 4 8.76274525553595 1104 5 8.702487978661456 1104 6 7.979956726652379 1104 7 7.6476295019945795 1104 8 6.817419771997958 1104 9 6.7277769174698285 1104 10-11 6.19681682969912 1104 12-13 6.001120514210751 1104 14-15 6.579294922460477 1104 16-17 7.20665202876765 1104 18-19 7.823714106970787 1104 20-21 8.105096052890769 1104 22-23 7.956455687931022 1104 24-25 7.867378904777919 1104 26-27 7.944214496840804 1104 28-29 7.666811130426396 1104 30-31 7.478400105153085 1104 32-33 7.461310729402765 1104 34-35 10.005295711109994 1104 36-37 -3.4879288146558913 1104 38-39 -2.1704652313763084 1104 40-41 5.983749092494445 1104 42-43 8.340500419279785 1104 44-45 0.2921939110911502 1104 46-47 -0.37142211540739467 1104 48-49 6.748341477554437 1104 50-51 -5.784364199848241 1104 52-53 3.646876007796532 1104 54-55 6.338067400938744 1104 56-57 7.036643805477173 1104 58-59 7.223509951835716 1104 60-61 7.1691982044231075 1104 62-63 7.231552728442608 1104 64-65 7.326126870876831 1104 66-67 7.301826096475217 1104 68-69 7.140757397436939 1104 70-71 7.150327631412498 1104 72-73 6.940618063255702 1104 74-75 6.789898119185171 1104 76-77 6.760543881616286 1104 78-79 6.662977019776296 1104 80-81 6.556318362618184 1104 82-83 6.291780073712156 1104 84-85 6.289206391649772 1104 86-87 6.094763357974619 1104 88-89 6.021357920886382 1104 90-91 6.019523503235819 1104 92-93 5.839358625938216 1104 94-95 5.631431366795736 1104 96-97 5.350203961058595 1104 98-99 4.848218395254873 1104 100-101 4.557301516079443 1105 1 5.695202724095601 1105 2 6.86841442920133 1105 3 6.425020170761947 1105 4 8.33362788702799 1105 5 8.31187392164519 1105 6 7.38962537732041 1105 7 7.248086242214413 1105 8 6.226614777423013 1105 9 6.211974528270073 1105 10-11 5.617778884340421 1105 12-13 5.336791723041745 1105 14-15 5.5911804917713965 1105 16-17 6.3037050285172285 1105 18-19 6.96066704073203 1105 20-21 7.215919684329062 1105 22-23 7.063569841656207 1105 24-25 7.154088423611359 1105 26-27 7.227325497572483 1105 28-29 7.072452001594243 1105 30-31 6.82649003334916 1105 32-33 6.562192869191424 1105 34-35 8.835836234539116 1105 36-37 -4.048616651110997 1105 38-39 -2.661376061839377 1105 40-41 5.057802014137163 1105 42-43 7.29747844079159 1105 44-45 -3.7826674927393924 1105 46-47 -4.711578431391647 1105 48-49 4.306113741437279 1105 50-51 -10.207661410388882 1105 52-53 -0.08498682784813028 1105 54-55 4.55424816241322 1105 56-57 5.698402195492374 1105 58-59 6.141565191011335 1105 60-61 6.054290389015517 1105 62-63 6.104064975881673 1105 64-65 5.990502275960996 1105 66-67 5.744268626467491 1105 68-69 5.677114891464157 1105 70-71 5.669285842499889 1105 72-73 5.649381120319184 1105 74-75 5.593278938880804 1105 76-77 5.343766146554334 1105 78-79 5.227835904170956 1105 80-81 5.201971345789092 1105 82-83 5.178690527347685 1105 84-85 5.113473236896143 1105 86-87 4.998657648710994 1105 88-89 4.84497374868751 1105 90-91 4.793518791150992 1105 92-93 4.688269872826178 1105 94-95 4.443052025473746 1105 96-97 4.21630823654999 1105 98-99 4.076379368061717 1105 100-101 3.797357848751153 1106 1 5.626646539922792 1106 2 6.903152423473728 1106 3 6.458212552396915 1106 4 7.8283832008417065 1106 5 7.746331381904362 1106 6 6.950657483780919 1106 7 6.7301566405354265 1106 8 5.9371306842345675 1106 9 5.872784657663114 1106 10-11 5.441743264357822 1106 12-13 5.205768055143789 1106 14-15 5.608096854987949 1106 16-17 6.103691503221597 1106 18-19 6.466363465471819 1106 20-21 6.873949438504713 1106 22-23 6.652700433757619 1106 24-25 6.845373972412343 1106 26-27 6.785021206440714 1106 28-29 6.748052473631272 1106 30-31 6.208507478663094 1106 32-33 6.412261017088635 1106 34-35 8.78285136296148 1106 36-37 -0.43343485438924034 1106 38-39 0.47212403080468235 1106 40-41 6.168457418993814 1106 42-43 7.29857584367149 1106 44-45 -3.8702654813467543 1106 46-47 -4.8243619635647335 1106 48-49 4.14534048006189 1106 50-51 -10.11050012134394 1106 52-53 -0.10280835356678963 1106 54-55 4.423112415741581 1106 56-57 5.4742196305464645 1106 58-59 5.860447873081668 1106 60-61 5.822725688225287 1106 62-63 5.837206047769776 1106 64-65 5.69185089751322 1106 66-67 5.730853618887952 1106 68-69 5.481136412695449 1106 70-71 5.439817667868098 1106 72-73 5.36805739415923 1106 74-75 5.286422477765978 1106 76-77 5.131412685074093 1106 78-79 4.994918937161618 1106 80-81 4.716890305146107 1106 82-83 4.571978314985374 1106 84-85 4.359157019065991 1106 86-87 4.31372988421799 1106 88-89 4.380659725031093 1106 90-91 4.181942370544634 1106 92-93 4.162562857484973 1106 94-95 4.074379696511587 1106 96-97 3.6987269849692748 1106 98-99 3.4031978593318453 1106 100-101 3.1620580174908035 1107 1 5.570456610685646 1107 2 6.854244757902297 1107 3 6.321357761439099 1107 4 7.65217276187888 1107 5 8.046572399860306 1107 6 6.5458097974805725 1107 7 6.7219516235803844 1107 8 6.015184509563689 1107 9 5.921204367116179 1107 10-11 5.485416566060049 1107 12-13 5.0139764623900795 1107 14-15 5.458832420157179 1107 16-17 6.328011045649049 1107 18-19 6.579964904266365 1107 20-21 7.053027336642952 1107 22-23 6.953596054474371 1107 24-25 6.883282293815636 1107 26-27 6.897627630602198 1107 28-29 6.875869116847571 1107 30-31 6.69113628181961 1107 32-33 6.332074432256022 1107 34-35 8.611297043479489 1107 36-37 -0.4948954061995998 1107 38-39 0.23221155562883666 1107 40-41 6.297968593281109 1107 42-43 7.501717127760173 1107 44-45 -3.7601795492425083 1107 46-47 -4.670393781294482 1107 48-49 4.379498371149143 1107 50-51 -10.082481873587806 1107 52-53 -0.013050853622864622 1107 54-55 4.631054034721398 1107 56-57 5.662698007402025 1107 58-59 6.107133146404184 1107 60-61 6.189665667676639 1107 62-63 6.080055938886115 1107 64-65 6.121094341217432 1107 66-67 5.867955563102196 1107 68-69 5.781985020007845 1107 70-71 5.869133885044622 1107 72-73 5.855640529273195 1107 74-75 5.753956034735619 1107 76-77 5.7815605929315055 1107 78-79 5.641634303159943 1107 80-81 5.418531513388549 1107 82-83 5.133217473607269 1107 84-85 4.958105485921999 1107 86-87 4.7935131985155035 1107 88-89 4.774653409256496 1107 90-91 4.6359782664567515 1107 92-93 4.535759102189436 1107 94-95 4.338015965171378 1107 96-97 4.2279661472784955 1107 98-99 4.183376649567355 1107 100-101 3.7519337679256264 1108 1 5.756230736220921 1108 2 6.3073721800887235 1108 3 6.495155886496722 1108 4 8.132590766015543 1108 5 8.204984187494642 1108 6 7.615364228227751 1108 7 7.5647032639146445 1108 8 6.628412285831882 1108 9 4.792759963017534 1108 10-11 5.779911923382347 1108 12-13 5.152029445224027 1108 14-15 5.955701372941363 1108 16-17 6.039648283575673 1108 18-19 6.7985928641485565 1108 20-21 7.596284243796301 1108 22-23 7.625123560628797 1108 24-25 7.334063279490575 1108 26-27 7.573592159198771 1108 28-29 7.42007098556207 1108 30-31 7.081169407345001 1108 32-33 7.332591582808266 1108 34-35 9.742074429848863 1108 36-37 -3.64480934306005 1108 38-39 -2.237270012365954 1108 40-41 5.650849567624025 1108 42-43 8.12795708412714 1108 44-45 -3.236322861851912 1108 46-47 -4.246732094084628 1108 48-49 5.271714586856735 1108 50-51 -10.106914605565656 1108 52-53 0.5595131977018646 1108 54-55 5.420139484144489 1108 56-57 6.7139881286249015 1108 58-59 7.137197603556199 1108 60-61 7.1327888379845135 1108 62-63 7.2429247526262195 1108 64-65 7.199123098971647 1108 66-67 7.042055283159215 1108 68-69 7.103204459588454 1108 70-71 6.961644499848255 1108 72-73 6.916317513497852 1108 74-75 6.882769017336699 1108 76-77 6.846377641657991 1108 78-79 6.774921641605822 1108 80-81 6.592309245540372 1108 82-83 6.48322440211817 1108 84-85 6.375050274591926 1108 86-87 6.234684759276478 1108 88-89 6.216044705692921 1108 90-91 6.3275000605667255 1108 92-93 6.214438273481315 1108 94-95 5.888217189842374 1108 96-97 5.627161983077594 1108 98-99 5.405573097187476 1108 100-101 4.808500693143875 1109 1 4.892869092527381 1109 2 6.51130109282138 1109 3 6.200264277843733 1109 4 7.899944123355777 1109 5 7.7728249001318055 1109 6 5.285113415183215 1109 7 4.882929873857329 1109 8 3.243227172982177 1109 9 2.9738593797266866 1109 10-11 2.7072908994546943 1109 12-13 3.1992268239509194 1109 14-15 3.9532275051930164 1109 16-17 3.0918196125507684 1109 18-19 3.944346175423558 1109 20-21 4.461273811773154 1109 22-23 5.939774744484957 1109 24-25 6.005479384540848 1109 26-27 4.85054319671028 1109 28-29 4.699719650646987 1109 30-31 4.851086995035622 1109 32-33 4.373648059181988 1109 34-35 6.790303444073185 1109 36-37 -4.682507216449364 1109 38-39 -3.394434418087469 1109 40-41 3.52104351300979 1109 42-43 4.320054422513284 1109 44-45 -4.62318140860172 1109 46-47 -6.1019180353002795 1109 48-49 2.191212418625714 1109 50-51 -10.354098094009949 1109 52-53 -1.1429146216550947 1109 54-55 2.651055902694228 1109 56-57 4.149793017465601 1109 58-59 4.9290157725172605 1109 60-61 5.156017650168362 1109 62-63 5.160639526701193 1109 64-65 4.804165237879861 1109 66-67 4.983466088014385 1109 68-69 5.034927507894313 1109 70-71 5.017740970133826 1109 72-73 4.682708131142313 1109 74-75 4.942711311209958 1109 76-77 5.109545629557115 1109 78-79 5.0693827861920475 1109 80-81 4.8510412159535665 1109 82-83 4.792992705469819 1109 84-85 4.801861651625716 1109 86-87 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2313 82-83 -13.21794788113113 2313 84-85 -12.683948705928664 2313 86-87 -12.1201444087649 2313 88-89 -11.538949832569267 2313 90-91 -10.962211074563887 2313 92-93 -10.347402683007626 2313 94-95 -9.850485969599214 2313 96-97 -9.073708369637995 2313 98-99 -8.190144937477523 2313 100-101 -7.063211199005863 2314 1 -20.938111659076654 2314 2 -19.744244278397684 2314 3 -20.198819131872124 2314 4 -22.148085207533317 2314 5 -22.106735136531807 2314 6 -22.886288967951526 2314 7 -22.987464259964852 2314 8 -23.842932313727264 2314 9 -25.034756568944257 2314 10-11 -25.324423124376654 2314 12-13 -25.305997740668698 2314 14-15 -25.543839405897728 2314 16-17 -24.633569938319994 2314 18-19 -23.96646958992933 2314 20-21 -23.44752545495462 2314 22-23 -23.381948445249233 2314 24-25 -23.11367735239774 2314 26-27 -22.81114332941254 2314 28-29 -22.763250101873638 2314 30-31 -22.700608696990045 2314 32-33 -22.443492179131486 2314 34-35 -19.56799169799831 2314 36-37 -17.88498201021655 2314 38-39 -16.994709395172713 2314 40-41 -18.402971152434755 2314 42-43 -18.33649919801834 2314 44-45 -16.355720363688796 2314 46-47 -17.204851124209537 2314 48-49 -16.695497022254212 2314 50-51 -14.641999102259263 2314 52-53 -17.599009947044646 2314 54-55 -19.293582044143538 2314 56-57 -19.530041996283256 2314 58-59 -19.14751951620868 2314 60-61 -18.84783202902504 2314 62-63 -18.381248649284142 2314 64-65 -17.89134346972784 2314 66-67 -17.467404672755553 2314 68-69 -17.02868385782521 2314 70-71 -16.446878644898256 2314 72-73 -15.97815346372478 2314 74-75 -15.43675706642524 2314 76-77 -14.873497450186239 2314 78-79 -14.380938020407402 2314 80-81 -13.872881790462566 2314 82-83 -13.399857890313866 2314 84-85 -12.87439866001498 2314 86-87 -12.310135226028445 2314 88-89 -11.729216131926476 2314 90-91 -11.149630725619902 2314 92-93 -10.509569808811117 2314 94-95 -9.850485969599214 2314 96-97 -9.111908553292723 2314 98-99 -8.279125653730967 2314 100-101 -7.16275206218309 2315 1 -20.938111659076654 2315 2 -19.744244278397684 2315 3 -20.198819131872124 2315 4 -16.082808688947644 2315 5 -22.106735136531807 2315 6 -22.886288967951526 2315 7 -22.987464259964852 2315 8 -23.842932313727264 2315 9 -19.42006935226248 2315 10-11 -17.48054279799406 2315 12-13 -13.411618774213576 2315 14-15 -13.22117394805548 2315 16-17 -19.641548179480466 2315 18-19 -23.96646958992933 2315 20-21 -23.44752545495462 2315 22-23 -23.381948445249233 2315 24-25 -23.11367735239774 2315 26-27 -22.81114332941254 2315 28-29 -22.763250101873638 2315 30-31 -22.700608696990045 2315 32-33 -22.443492179131486 2315 34-35 -19.56799169799831 2315 36-37 -17.88498201021655 2315 38-39 -16.994709395172713 2315 40-41 -18.402971152434755 2315 42-43 -18.33649919801834 2315 44-45 -16.355720363688796 2315 46-47 -17.204851124209537 2315 48-49 -16.695497022254212 2315 50-51 -14.641999102259263 2315 52-53 -17.599009947044646 2315 54-55 -19.293582044143538 2315 56-57 -19.530041996283256 2315 58-59 -19.14751951620868 2315 60-61 -18.84783202902504 2315 62-63 -18.381248649284142 2315 64-65 -17.89134346972784 2315 66-67 -17.467404672755553 2315 68-69 -17.02868385782521 2315 70-71 -16.446878644898256 2315 72-73 -15.97815346372478 2315 74-75 -15.43675706642524 2315 76-77 -14.873497450186239 2315 78-79 -14.380938020407402 2315 80-81 -13.872881790462566 2315 82-83 -13.399857890313866 2315 84-85 -12.87439866001498 2315 86-87 -12.310135226028445 2315 88-89 -11.729216131926476 2315 90-91 -11.149630725619902 2315 92-93 -10.509569808811117 2315 94-95 -9.850485969599214 2315 96-97 -9.111908553292723 2315 98-99 -8.279125653730967 2315 100-101 -7.16275206218309 2316 1 -20.938111659076654 2316 2 -19.744244278397684 2316 3 -20.198819131872124 2316 4 -22.148085207533317 2316 5 -22.106735136531807 2316 6 -22.886288967951526 2316 7 -22.987464259964852 2316 8 -23.842932313727264 2316 9 -25.034756568944257 2316 10-11 -25.324423124376654 2316 12-13 -25.305997740668698 2316 14-15 -25.543839405897728 2316 16-17 -24.633569938319994 2316 18-19 -23.96646958992933 2316 20-21 -23.44752545495462 2316 22-23 -23.381948445249233 2316 24-25 -23.11367735239774 2316 26-27 -22.81114332941254 2316 28-29 -22.763250101873638 2316 30-31 -22.700608696990045 2316 32-33 -22.443492179131486 2316 34-35 -19.56799169799831 2316 36-37 -17.88498201021655 2316 38-39 -16.994709395172713 2316 40-41 -18.402971152434755 2316 42-43 -18.33649919801834 2316 44-45 -16.355720363688796 2316 46-47 -17.204851124209537 2316 48-49 -16.695497022254212 2316 50-51 -14.641999102259263 2316 52-53 -17.599009947044646 2316 54-55 -19.293582044143538 2316 56-57 -19.530041996283256 2316 58-59 -19.14751951620868 2316 60-61 -18.84783202902504 2316 62-63 -18.381248649284142 2316 64-65 -17.89134346972784 2316 66-67 -17.467404672755553 2316 68-69 -17.02868385782521 2316 70-71 -16.446878644898256 2316 72-73 -15.97815346372478 2316 74-75 -15.43675706642524 2316 76-77 -14.873497450186239 2316 78-79 -14.380938020407402 2316 80-81 -13.872881790462566 2316 82-83 -13.399857890313866 2316 84-85 -12.87439866001498 2316 86-87 -12.310135226028445 2316 88-89 -11.729216131926476 2316 90-91 -11.149630725619902 2316 92-93 -10.509569808811117 2316 94-95 -9.850485969599214 2316 96-97 -9.111908553292723 2316 98-99 -8.279125653730967 2316 100-101 -7.16275206218309 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 825421.0 3 50052.0 4 112407.0 5 61407.0 6 52689.0 7 63654.0 8 82227.0 9 97965.0 10 102647.0 11 96763.0 12 86052.0 13 79634.0 14 77741.0 15 83030.0 16 91542.0 17 98320.0 18 102154.0 19 106939.0 20 112809.0 21 120342.0 22 127148.0 23 135181.0 24 143561.0 25 152789.0 26 162979.0 27 173400.0 28 184170.0 29 195667.0 30 204823.0 31 205987.0 32 199195.0 33 195225.0 34 182240.0 35 156628.0 36 114185.0 37 53588.0 38 7971.0 39 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.755470812311685 20.689655172413794 13.842640689798024 23.712233325476497 2 14.952372207456676 20.990517207764846 41.4361166211122 22.620993963666276 3 18.163832960708493 26.50043615353845 30.60827019622258 24.727460689530478 4 12.23436096786099 17.208591249441742 39.85105161416864 30.70599616852863 5 12.819065991316048 38.06246823472135 36.11914614930979 12.999319624652811 6 29.774822846108545 38.35543569289026 18.549152820033864 13.32058864096733 7 25.833847645610746 32.861628867616716 24.065031362600138 17.239492124172397 8 24.285328362341374 36.75514987518341 21.563575751393607 17.395946011081605 9 25.574040544531773 16.552847788047316 21.875750785273283 35.997360882147625 10-11 24.18668374289198 26.789110581465675 29.6717863075665 19.35241936807585 12-13 24.39342402473476 24.600208974497374 29.63154496516073 21.374822035607135 14-15 21.70793091254669 26.209238691557964 26.89109435219675 25.191736043698604 16-17 21.621424529185212 29.176282733920523 27.620425411957722 21.58186732493655 18-19 21.550365453000765 28.34115674401709 29.263062193863398 20.84541560911874 20-21 22.155040648206842 27.558335817322682 29.173592710204428 21.113030824266048 22-23 21.998335211411163 27.45872853187164 28.866007168783515 21.67692908793368 24-25 21.782225149845456 27.705676883018388 28.842328419394015 21.669769547742142 26-27 21.784730368038456 27.811948351855758 28.72283973708723 21.680481543018555 28-29 21.822881325768147 27.997461746529684 28.703019583047208 21.476637344654968 30-31 22.024415347313457 27.739606441776687 28.56692785651865 21.6690503543912 32-33 21.862135125318815 27.93872162160824 28.486025204634068 21.713118048438876 34-35 22.241859362122796 27.88728132447753 28.47161682967338 21.399242483726294 36-37 22.732262555191742 27.744765820485302 28.073431986874137 21.449539637448822 38-39 22.491339451560375 27.853639649107194 28.113517022076945 21.54150387725549 40-41 22.324618184270033 27.770664620328873 28.35266229166272 21.552054903738373 42-43 22.50571597903159 27.801299804411006 27.947024273791328 21.74595994276608 44-45 22.767269577125596 27.984356443488583 27.684503543815804 21.563870435570017 46-47 22.202122025520435 27.91578211215774 28.057235391836898 21.824860470484925 48-49 22.643530651630655 27.89951376328899 27.615211080370322 21.841744504710032 50-51 22.82189798979153 28.048412423451214 27.424549001817272 21.70514058493998 52-53 21.921400748197 28.06791278733558 28.02627836622797 21.98440809823945 54-55 21.7500432437034 27.97410268183411 28.171518112093114 22.104335962369376 56-57 21.803459119850825 28.198488202490907 27.934575372293136 22.063477305365133 58-59 21.91282104881865 27.97970153431863 28.077644526322594 22.02983289054013 60-61 21.820032799093667 28.11949599405135 28.05189454944254 22.008576657412448 62-63 21.846152036396038 28.022425613864193 27.982497832249475 22.14892451749029 64-65 21.64179745640886 27.93561670245922 27.991622399942077 22.430963441189835 66-67 21.68630638728376 27.926490319945778 27.998337611242206 22.388865681528255 68-69 21.77620271249447 27.920276823221485 28.016373247246957 22.287147217037095 70-71 21.737564226746485 27.725108015231786 27.981629162597187 22.555698595424538 72-73 21.86191538133416 27.785759492722008 27.888429136678784 22.463895989265055 74-75 21.781492802183433 28.00902028987311 27.77636696055996 22.433119947383496 76-77 21.790081264947563 27.838258562525624 27.85791441610108 22.513745756425728 78-79 21.676539238208232 27.884983657285407 27.79646504117819 22.642012063328174 80-81 21.820169277912076 27.892799070471625 27.755541275244216 22.53149037637209 82-83 21.889551150349696 27.79421052596917 27.837962077744287 22.478276245936847 84-85 21.94834086777952 27.759170505193666 27.692243560982945 22.600245066043872 86-87 21.73714185564379 27.86240676697104 27.696687223386675 22.703764153998492 88-89 21.84813140834441 27.683210335853136 27.546469954078905 22.92218830172354 90-91 21.82258704483918 27.64490552014521 27.679008210461326 22.853499224554284 92-93 21.914080383922947 27.465763639050866 27.67299124307358 22.9471647339526 94-95 21.68416728162524 27.45371804563554 27.78534550112093 23.07676917161829 96-97 21.79198752806357 27.23081825749477 27.628856430029785 23.34833778441187 98-99 21.68458615164183 27.351674336351966 27.541434737900346 23.422304774105854 100-101 21.93471462511741 27.369313221724955 27.417978893861537 23.277993259296093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 64276.0 1 69284.0 2 51819.0 3 31347.5 4 40571.5 5 50731.0 6 53187.0 7 50304.5 8 45740.5 9 40966.0 10 35975.0 11 30962.5 12 25821.5 13 21377.5 14 18010.5 15 15464.0 16 13707.0 17 12685.5 18 12237.5 19 12446.5 20 13141.5 21 14498.0 22 16611.0 23 19493.0 24 23637.0 25 29365.5 26 37317.5 27 47767.0 28 60770.5 29 77077.0 30 95022.5 31 112314.5 32 128699.5 33 140399.5 34 148265.5 35 154402.0 36 158844.5 37 161572.0 38 159864.5 39 154184.5 40 148003.0 41 143622.5 42 140533.5 43 138287.5 44 137111.5 45 137642.5 46 138369.5 47 138266.0 48 137099.0 49 135065.5 50 131317.5 51 126503.0 52 122381.0 53 117620.5 54 111099.5 55 102608.0 56 92867.5 57 82949.0 58 72934.5 59 62855.0 60 53356.0 61 44476.5 62 36601.0 63 29535.5 64 22979.0 65 17782.5 66 13734.0 67 10543.0 68 8249.5 69 6384.0 70 4928.5 71 3811.0 72 2903.0 73 2222.5 74 1713.5 75 1325.5 76 969.0 77 663.0 78 449.5 79 289.5 80 191.0 81 120.5 82 71.5 83 43.5 84 30.0 85 24.0 86 13.0 87 7.0 88 4.5 89 1.5 90 1.5 91 2.5 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.875079139557853 2 22.035375284294396 3 17.774666240927882 4 23.1024942591949 5 22.973890026022893 6 16.67171381566818 7 15.531084292357022 8 12.514111750155141 9 3.1791154727544173 10-11 6.797215229000657 12-13 3.7049081039691774 14-15 7.706055088193045 16-17 11.917485782338169 18-19 15.067093085379717 20-21 15.547020076931103 22-23 13.407888615436272 24-25 16.004607550168792 26-27 13.437230072325907 28-29 13.487675411309954 30-31 14.400104499520259 32-33 12.842810597757653 34-35 19.692167713884707 36-37 24.504696202164208 38-39 23.536290831893066 40-41 18.697264811355616 42-43 18.639444881602515 44-45 24.007068231439764 46-47 13.340320888842166 48-49 22.040906227371185 50-51 21.79067027969312 52-53 11.766130152112703 54-55 13.371545219970708 56-57 12.877898743730617 58-59 13.961757647411401 60-61 13.352383051935163 62-63 13.299897932525633 64-65 14.393269273767562 66-67 12.740164531827244 68-69 13.78818998514883 70-71 12.313340477795029 72-73 11.695071262867287 74-75 11.760501142669304 76-77 11.79149992115464 78-79 12.201976311873013 80-81 12.206114712543943 82-83 10.656303274553661 84-85 10.603582795864266 86-87 10.697344048979836 88-89 12.18229439209444 90-91 9.590518002239051 92-93 11.685774571312685 94-95 11.774191207448023 96-97 11.280623184301222 98-99 11.174054463706423 100-101 11.254841536519521 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5098588.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 95.36866816691058 #Duplication Level Percentage of deduplicated Percentage of total 1 98.33814954404664 93.78378352014212 2 1.0989789967655728 2.0961632652988045 3 0.2577005770286966 0.7372968245121344 4 0.0993344806601414 0.3789358849443769 5 0.05479792183315968 0.2613002411771455 6 0.02850477197232437 0.16310772836412335 7 0.02357309887902674 0.15736945312617712 8 0.016385332904107992 0.12501179012289892 9 0.010968031955246944 0.09414059399856158 >10 0.06500375878734567 1.0939842286684252 >50 0.0035392503033119557 0.23637075610482428 >100 0.002797005002606789 0.5099003633564467 >500 2.0556189207289832E-4 0.12295716695398776 >1k 4.111197973117901E-5 0.057518059021606935 >5k 2.055598986551545E-5 0.18216012420827885 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 9292 0.18224653570753313 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.922654664389435E-5 2 0.0 0.0 0.0 0.0 3.922654664389435E-5 3 0.0 0.0 0.0 0.0 3.922654664389435E-5 4 0.0 0.0 0.0 5.8839819965841526E-5 3.922654664389435E-5 5 0.0 0.0 0.0 5.8839819965841526E-5 3.922654664389435E-5 6 0.0 0.0 0.0 7.84530932877887E-5 5.8839819965841526E-5 7 0.0 3.922654664389435E-5 0.0 7.84530932877887E-5 5.8839819965841526E-5 8 0.0 3.922654664389435E-5 0.0 7.84530932877887E-5 5.8839819965841526E-5 9 0.0 3.922654664389435E-5 0.0 9.806636660973587E-5 7.84530932877887E-5 10-11 0.0 3.922654664389435E-5 0.0 9.806636660973587E-5 7.84530932877887E-5 12-13 0.0 4.903318330486794E-5 0.0 1.1767963993168305E-4 1.7651945989752456E-4 14-15 0.0 8.825972994876229E-5 0.0 1.3729291325363023E-4 2.451659165243397E-4 16-17 0.0 1.3729291325363023E-4 0.0 1.569061865755774E-4 2.7458582650726046E-4 18-19 0.0 1.3729291325363023E-4 0.0 1.8632609655849815E-4 2.7458582650726046E-4 20-21 0.0 1.3729291325363023E-4 0.0 2.0593936988044532E-4 2.7458582650726046E-4 22-23 0.0 1.3729291325363023E-4 0.0 2.353592798633661E-4 2.8439246316823403E-4 24-25 0.0 1.3729291325363023E-4 0.0 2.5497255318531327E-4 3.138123731511548E-4 26-27 0.0 1.3729291325363023E-4 0.0 4.51105286404785E-4 3.138123731511548E-4 28-29 0.0 1.3729291325363023E-4 0.0 9.904703027583323E-4 3.138123731511548E-4 30-31 0.0 1.3729291325363023E-4 0.0 0.002804698085038446 3.138123731511548E-4 32-33 0.0 1.3729291325363023E-4 0.0 0.008727906628266491 3.138123731511548E-4 34-35 0.0 1.3729291325363023E-4 0.0 0.018897388845696104 3.138123731511548E-4 36-37 0.0 1.3729291325363023E-4 0.0 0.03475472032649039 3.138123731511548E-4 38-39 0.0 1.3729291325363023E-4 0.0 0.05733940455671256 3.2361900981212836E-4 40-41 0.0 1.3729291325363023E-4 0.0 0.0821698085822977 3.33425646473102E-4 42-43 0.0 1.3729291325363023E-4 0.0 0.1084908213803508 3.33425646473102E-4 44-45 0.0 1.3729291325363023E-4 0.0 0.1417941594810171 3.4323228313407555E-4 46-47 3.922654664389435E-5 1.3729291325363023E-4 0.0 0.18413921658310106 3.726521931169963E-4 48-49 3.922654664389435E-5 1.3729291325363023E-4 0.0 0.22862212047727726 4.2168537642186426E-4 50-51 3.922654664389435E-5 1.3729291325363023E-4 0.0 0.28130337262002736 4.707185597267322E-4 52-53 3.922654664389435E-5 1.3729291325363023E-4 0.0 0.35132275837937876 5.197517430316002E-4 54-55 3.922654664389435E-5 1.3729291325363023E-4 0.0 0.4285402154478848 5.982048363193888E-4 56-57 3.922654664389435E-5 1.3729291325363023E-4 0.0 0.5200067155847854 6.080114729803624E-4 58-59 3.922654664389435E-5 1.470995499146038E-4 0.0 0.6202207356232745 6.080114729803624E-4 60-61 3.922654664389435E-5 1.7651945989752456E-4 0.0 0.7269561690413111 6.080114729803624E-4 62-63 3.922654664389435E-5 1.7651945989752456E-4 0.0 0.8345349732121913 6.66851292946204E-4 64-65 3.922654664389435E-5 1.7651945989752456E-4 0.0 0.9490568761390408 6.864645662681511E-4 66-67 3.922654664389435E-5 1.9613273321947175E-4 0.0 1.0790242318069239 7.060778395900982E-4 68-69 3.922654664389435E-5 1.9613273321947175E-4 0.0 1.2223090000604089 7.256911129120455E-4 70-71 3.922654664389435E-5 1.9613273321947175E-4 0.0 1.379725131742357 7.256911129120455E-4 72-73 3.922654664389435E-5 1.9613273321947175E-4 0.0 1.5384651593735363 7.943375695388605E-4 74-75 3.922654664389435E-5 1.9613273321947175E-4 0.0 1.7037364070209242 8.041442061998341E-4 76-77 3.922654664389435E-5 1.9613273321947175E-4 0.0 1.8878658169673643 8.237574795217813E-4 78-79 5.8839819965841526E-5 1.9613273321947175E-4 0.0 2.095138889433702 8.335641161827549E-4 80-81 5.8839819965841526E-5 2.255526432023925E-4 0.0 2.3207111459094163 8.727906628266493E-4 82-83 5.8839819965841526E-5 2.353592798633661E-4 0.0 2.5550015023767365 9.218238461315172E-4 84-85 5.8839819965841526E-5 2.353592798633661E-4 0.0 2.7925378555788387 9.218238461315172E-4 86-87 5.8839819965841526E-5 2.353592798633661E-4 0.0 3.0449802965056207 9.51243756114438E-4 88-89 5.8839819965841526E-5 2.353592798633661E-4 0.0 3.3148687440522746 9.806636660973588E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3375 0.0 47.729263 1 GTATCAA 6295 0.0 41.071636 1 TCAACGC 7125 0.0 35.19738 4 ATCAACG 7080 0.0 34.86205 3 CAACGCA 7450 0.0 32.939697 5 TATCAAC 7775 0.0 32.40142 2 AACGCAG 7940 0.0 31.051628 6 GTACATG 8805 0.0 25.979853 1 ACGCAGA 9385 0.0 25.562206 7 CGCAGAG 9830 0.0 24.764389 8 TACATGG 9360 0.0 24.541407 2 ACATGGG 9435 0.0 23.699495 3 GCAGAGT 11190 0.0 20.937466 9 CATGGGG 7035 0.0 19.581112 4 GAGTACA 7030 0.0 17.885942 1 CAGAGTA 10790 0.0 17.864424 10-11 AGAGTAC 10120 0.0 17.781702 10-11 GAGTACT 6570 0.0 17.724556 12-13 GTACTTT 7670 0.0 15.543198 14-15 ATGGGGG 4805 0.0 15.5129795 5 >>END_MODULE