FastQCFastQC Report
Sat 18 Jun 2016
SRR1930926_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930926_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5098588
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44400.049.2997631
GTATCAA86300.038.293521
ATCAACG97650.033.0069273
CAACGCA98050.032.7629135
TCAACGC98700.032.6057784
AACGCAG103500.031.1749486
TATCAAC108400.029.9975572
ACGCAGA121950.026.4195357
CGCAGAG126550.025.5341918
GTACATG128800.025.104881
ACATGGG126250.024.2889463
TACATGG133300.023.7887122
GCAGAGT149000.021.4321619
CATGGGG93400.020.175564
GAGTACA96000.019.6851481
AGAGTAC138350.019.05206510-11
TCTAACG4450.018.134832
CTAACGC4253.6379788E-1217.8705793
CAGAGTA148400.017.74582710-11
GAGTACT92850.017.11985412-13
Produced by FastQC (version 0.11.5)