##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930926_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5098588 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.283841134055155 31.0 31.0 34.0 30.0 34.0 2 31.41106184692703 31.0 31.0 34.0 30.0 34.0 3 31.499007372237177 31.0 31.0 34.0 30.0 34.0 4 35.35611094679547 37.0 35.0 37.0 33.0 37.0 5 35.14287426244286 37.0 35.0 37.0 32.0 37.0 6 35.176820523643016 37.0 35.0 37.0 32.0 37.0 7 35.060965310395744 37.0 35.0 37.0 32.0 37.0 8 35.04043727400606 37.0 35.0 37.0 32.0 37.0 9 36.55321198731884 38.0 35.0 39.0 32.0 39.0 10-11 36.48239532199895 38.0 35.0 39.0 32.0 39.0 12-13 36.38618123292174 38.0 35.0 39.0 32.0 39.0 14-15 37.42701802930537 39.0 36.0 41.0 32.0 41.0 16-17 37.39684879421518 39.0 36.0 41.0 32.0 41.0 18-19 37.37997657390635 39.0 36.0 41.0 32.0 41.0 20-21 37.33996167174127 39.0 36.0 40.5 31.5 41.0 22-23 37.217126192585084 39.0 36.0 40.0 31.0 41.0 24-25 37.103121982007565 39.0 36.0 40.0 31.0 41.0 26-27 36.83995863168391 39.0 36.0 40.0 30.5 41.0 28-29 36.664011290969185 38.5 35.0 40.0 30.0 41.0 30-31 36.44391996372329 38.0 35.0 40.0 30.0 41.0 32-33 36.22720721893983 38.0 35.0 40.0 30.0 41.0 34-35 36.43154673411541 38.0 35.0 40.0 30.0 41.0 36-37 36.48888898259675 38.0 35.0 40.0 30.0 41.0 38-39 36.456811277945974 38.5 35.0 40.0 30.0 41.0 40-41 36.372433210920356 38.0 35.0 40.0 30.0 41.0 42-43 36.249256558874734 38.0 35.0 40.0 30.0 41.0 44-45 36.090599299256965 38.0 35.0 40.0 29.5 41.0 46-47 35.90403401883031 38.0 35.0 40.0 29.0 41.0 48-49 35.72894054196966 38.0 34.0 40.0 28.0 41.0 50-51 35.47468985530896 38.0 34.0 40.0 27.5 41.0 52-53 35.2717007140016 38.0 34.0 40.0 27.0 41.0 54-55 35.11396125750895 38.0 34.0 40.0 27.0 41.0 56-57 34.87980946881764 38.0 33.0 40.0 26.0 41.0 58-59 34.65249516140547 37.5 33.0 40.0 26.0 41.0 60-61 34.35838696909811 37.0 33.0 40.0 26.0 41.0 62-63 34.071067028753845 37.0 32.5 40.0 25.5 41.0 64-65 33.78030113435328 36.0 32.0 39.0 25.0 41.0 66-67 33.467736557650866 36.0 32.0 39.0 24.5 40.5 68-69 33.11798776445557 35.5 31.5 39.0 23.5 40.0 70-71 32.732264403399526 35.0 31.0 38.5 23.0 40.0 72-73 32.33536235914728 35.0 31.0 38.0 22.0 40.0 74-75 31.823316082805672 35.0 30.5 37.0 21.0 39.5 76-77 30.42633705645563 33.5 28.5 35.5 20.0 38.5 78-79 31.212208066233238 34.0 30.0 36.0 20.5 39.0 80-81 31.2156652979217 34.0 30.0 36.0 21.0 38.5 82-83 30.931277247739963 34.0 30.0 35.5 20.0 37.0 84-85 30.59815139014959 34.0 30.0 35.0 20.0 37.0 86-87 30.24747586978983 34.0 30.0 35.0 18.0 36.5 88-89 29.88753886370109 34.0 29.5 35.0 17.5 36.0 90-91 29.546245450701253 34.0 29.0 35.0 13.0 36.0 92-93 29.18853739505918 34.0 29.0 35.0 7.0 35.5 94-95 28.778099544422886 34.0 29.0 35.0 2.0 35.0 96-97 28.32975933729103 33.0 29.0 35.0 2.0 35.0 98-99 27.848132071075362 33.0 29.0 35.0 2.0 35.0 100-101 26.634381126696255 32.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48584589375888143 1101 2 0.5884329334567475 1101 3 0.5441775852547117 1101 4 0.374751526235265 1101 5 0.5298113524175037 1101 6 0.43659658876231333 1101 7 0.5290457160168955 1101 8 0.5337934936325439 1101 9 0.6396728053825029 1101 10-11 0.5798479781924826 1101 12-13 0.5996905604454383 1101 14-15 0.6555949583088605 1101 16-17 0.7269463539304439 1101 18-19 0.7505996389448057 1101 20-21 0.7061888776459497 1101 22-23 0.8534017458259839 1101 24-25 0.780125482588538 1101 26-27 0.7759056441553582 1101 28-29 0.67817258468029 1101 30-31 0.7947535653553999 1101 32-33 0.7117158755231614 1101 34-35 0.6176319508753849 1101 36-37 0.6685107100084977 1101 38-39 0.7890799501356938 1101 40-41 0.8060462018112347 1101 42-43 0.7245808476709072 1101 44-45 0.7749218392591217 1101 46-47 0.8493465365059265 1101 48-49 0.7988222283131563 1101 50-51 0.7913736102227418 1101 52-53 0.8454573858129208 1101 54-55 0.8852108858735903 1101 56-57 0.9270623813962473 1101 58-59 0.8587570872746753 1101 60-61 0.8062209961440701 1101 62-63 0.734418322805503 1101 64-65 0.8425668188883364 1101 66-67 0.874144012836517 1101 68-69 0.8163834431152281 1101 70-71 0.7637239977829537 1101 72-73 0.7248512929115662 1101 74-75 0.7833250755271024 1101 76-77 0.9275974179069699 1101 78-79 0.9640236484822005 1101 80-81 0.9856659034534054 1101 82-83 1.0683368485096345 1101 84-85 1.1489933237727143 1101 86-87 1.0453733711950512 1101 88-89 0.9545832662834535 1101 90-91 0.873285001280486 1101 92-93 0.9891467074831048 1101 94-95 0.9828398288871689 1101 96-97 1.0215523968927585 1101 98-99 1.050464362963428 1101 100-101 1.2784990018394708 1102 1 0.51554041443854 1102 2 0.6042214871616487 1102 3 0.5442409861360709 1102 4 0.3290732077879639 1102 5 0.5063996896882514 1102 6 0.4741344276343753 1102 7 0.5143365502176209 1102 8 0.5286233372824185 1102 9 0.6929954471439714 1102 10-11 0.6521655159410145 1102 12-13 0.661525012516627 1102 14-15 0.7240274376608227 1102 16-17 0.7586381233667652 1102 18-19 0.8425937733146682 1102 20-21 0.8474631821237608 1102 22-23 0.8490155300810187 1102 24-25 0.8164845369442304 1102 26-27 0.9050245840474531 1102 28-29 0.8827631150085011 1102 30-31 0.8788664297330158 1102 32-33 0.9766907442526858 1102 34-35 0.8870264297072268 1102 36-37 0.8883585091751556 1102 38-39 0.9014269168633007 1102 40-41 0.9020342892212128 1102 42-43 0.8674243239622115 1102 44-45 0.934050247338817 1102 46-47 0.9897287518650018 1102 48-49 0.8415786614860394 1102 50-51 0.8911919457253745 1102 52-53 0.9298354086475626 1102 54-55 0.930380222690701 1102 56-57 0.8936517906742623 1102 58-59 0.9487969056093704 1102 60-61 0.7888987622132149 1102 62-63 0.7610691978667319 1102 64-65 0.859403402874662 1102 66-67 0.830370080213747 1102 68-69 0.8500184172999354 1102 70-71 0.8800244248758204 1102 72-73 0.7652982723516075 1102 74-75 0.9749959017002752 1102 76-77 1.086341569054671 1102 78-79 1.0987715944872356 1102 80-81 0.9764770507802716 1102 82-83 1.0835541086494196 1102 84-85 1.0218474943246356 1102 86-87 1.0851514350386111 1102 88-89 1.109860756453898 1102 90-91 1.0475490791800013 1102 92-93 1.125785871127075 1102 94-95 0.9990046740825456 1102 96-97 1.0622751822660952 1102 98-99 1.0934779084891346 1102 100-101 1.301678497155553 1103 1 0.38737430049005894 1103 2 0.507301183558738 1103 3 0.4537672168510092 1103 4 0.2643020988860414 1103 5 0.25392684497297324 1103 6 0.4346100182333643 1103 7 0.42638352999850326 1103 8 0.415764481522892 1103 9 0.5247835094430258 1103 10-11 0.5965159961860778 1103 12-13 0.5135456275136292 1103 14-15 0.6693768738123822 1103 16-17 0.68628658574778 1103 18-19 0.6727248668113148 1103 20-21 0.6776008367773372 1103 22-23 0.6910842929029357 1103 24-25 0.7263955044160681 1103 26-27 0.6604481312333945 1103 28-29 0.7834983440260785 1103 30-31 0.7761469329468724 1103 32-33 0.8357640496086063 1103 34-35 0.7131209097347551 1103 36-37 0.7592539126341293 1103 38-39 0.7666760890508257 1103 40-41 0.846851797360209 1103 42-43 0.8720832196894577 1103 44-45 0.8184738868552586 1103 46-47 0.7533187260377616 1103 48-49 0.9001805437633905 1103 50-51 0.8673064096140237 1103 52-53 0.6978888667408967 1103 54-55 0.8037094201236599 1103 56-57 0.8335738061857896 1103 58-59 0.8666464777270804 1103 60-61 0.7813785696598572 1103 62-63 0.6169808160788577 1103 64-65 0.6615583555837361 1103 66-67 0.5770002541998167 1103 68-69 0.5926847829438913 1103 70-71 0.5481959679840358 1103 72-73 0.5608148361899268 1103 74-75 0.6631855346296334 1103 76-77 0.7635128851310569 1103 78-79 0.9060948416622985 1103 80-81 0.8372053486865205 1103 82-83 0.8475836012870097 1103 84-85 0.9622638100089311 1103 86-87 0.9417009083753314 1103 88-89 0.8694045520974178 1103 90-91 0.9074999195140094 1103 92-93 0.8473796188346192 1103 94-95 1.0039650668286555 1103 96-97 0.9565960503498445 1103 98-99 0.9107128503968092 1103 100-101 0.9236903249452588 1104 1 0.4528035947278042 1104 2 0.4878461110317325 1104 3 0.5208439017619177 1104 4 0.2734389925285541 1104 5 0.4598690869878226 1104 6 0.4900006604907361 1104 7 0.4905054110009459 1104 8 0.5325133245542162 1104 9 0.6100367153666326 1104 10-11 0.5606179239672358 1104 12-13 0.5653058288820816 1104 14-15 0.6540598221258165 1104 16-17 0.6674056796624583 1104 18-19 0.7107627970213954 1104 20-21 0.7354554064286276 1104 22-23 0.6535098546611096 1104 24-25 0.7175896115344145 1104 26-27 0.7418942034486022 1104 28-29 0.6823540336030405 1104 30-31 0.6639676730250343 1104 32-33 0.8543407713352593 1104 34-35 0.674490291584867 1104 36-37 0.7842742976449486 1104 38-39 0.734930127646443 1104 40-41 0.6827701864125686 1104 42-43 0.7375710100451869 1104 44-45 0.6919112632839131 1104 46-47 0.6239750370280319 1104 48-49 0.7140274430146221 1104 50-51 0.6695814631786519 1104 52-53 0.7959676987820501 1104 54-55 0.6781530934071611 1104 56-57 0.7477377980299664 1104 58-59 0.7265548656249763 1104 60-61 0.7075386972150213 1104 62-63 0.6163047245831095 1104 64-65 0.6930591059884819 1104 66-67 0.6983278842752796 1104 68-69 0.6828090292439057 1104 70-71 0.599353368447467 1104 72-73 0.6277040335431963 1104 74-75 0.8167009149725715 1104 76-77 0.8694198340335362 1104 78-79 0.893565786772303 1104 80-81 0.7448209114719013 1104 82-83 0.9584853323539484 1104 84-85 0.9709757318541143 1104 86-87 0.9428070849654304 1104 88-89 1.0588784551929393 1104 90-91 0.9670939191849399 1104 92-93 0.977502687940035 1104 94-95 0.9927555834580026 1104 96-97 0.9976584389367851 1104 98-99 1.175803439839509 1104 100-101 1.440673288803115 1105 1 0.15434943616826047 1105 2 0.32377404994389636 1105 3 0.25114329472581787 1105 4 0.07082489286898408 1105 5 0.15900634337436514 1105 6 0.18773634468837486 1105 7 0.2071712400313288 1105 8 0.22241961154755785 1105 9 0.334445458374816 1105 10-11 0.22141961368630092 1105 12-13 0.2550733075772911 1105 14-15 0.36020820297488854 1105 16-17 0.32677958026483367 1105 18-19 0.3605649625613907 1105 20-21 0.33016302458919 1105 22-23 0.37307510184975 1105 24-25 0.36303770016808556 1105 26-27 0.342904817061779 1105 28-29 0.4243323064057307 1105 30-31 0.3934168651629122 1105 32-33 0.45266414985091075 1105 34-35 0.43477646512526036 1105 36-37 0.5199174293429039 1105 38-39 0.33438099723257153 1105 40-41 0.446561378264569 1105 42-43 0.4265892394039241 1105 44-45 0.4868355464032348 1105 46-47 0.39108718477750415 1105 48-49 0.30500594796222913 1105 50-51 0.3698163494461397 1105 52-53 0.4204160282633467 1105 54-55 0.4883091396072601 1105 56-57 0.4088323340958695 1105 58-59 0.29717647007049663 1105 60-61 0.36865373905224175 1105 62-63 0.3392025131580567 1105 64-65 0.4724021576432307 1105 66-67 0.44386839573816417 1105 68-69 0.45498800622674196 1105 70-71 0.3218694342025614 1105 72-73 0.3196355510116078 1105 74-75 0.40686125596233325 1105 76-77 0.42789321036263317 1105 78-79 0.527151205112883 1105 80-81 0.4919168156045366 1105 82-83 0.5072319565165557 1105 84-85 0.5518511466200557 1105 86-87 0.5564525172260026 1105 88-89 0.5336462395710022 1105 90-91 0.44689948748634833 1105 92-93 0.5196487925940936 1105 94-95 0.3673015465188989 1105 96-97 0.28858458617757776 1105 98-99 0.4837240850065534 1105 100-101 0.7402330728626545 1106 1 0.08700420836973066 1106 2 0.13661534903379646 1106 3 0.16026257782616327 1106 4 0.030721443318178387 1106 5 0.08840777636047648 1106 6 0.08066888264162486 1106 7 0.11614926595544972 1106 8 0.09218397119275323 1106 9 0.10849644136570191 1106 10-11 0.13821418603994573 1106 12-13 0.15570430896637788 1106 14-15 0.12300406486644988 1106 16-17 0.16357366174715793 1106 18-19 0.14979040027166235 1106 20-21 0.15124206087606495 1106 22-23 0.2167455426427196 1106 24-25 0.12456609228114957 1106 26-27 0.25971871879039554 1106 28-29 0.22173759852525876 1106 30-31 0.11627007472925044 1106 32-33 0.22901069362699644 1106 34-35 0.09234463171983265 1106 36-37 0.2670791450034855 1106 38-39 0.16742233586604982 1106 40-41 0.11323252176195098 1106 42-43 0.177418901472727 1106 44-45 0.06553364821647278 1106 46-47 0.10334152395883933 1106 48-49 0.17991081530951192 1106 50-51 0.0813837847899066 1106 52-53 -0.024736738581310647 1106 54-55 0.11321535000377025 1106 56-57 0.2877977922394521 1106 58-59 0.28823076159728345 1106 60-61 0.10667255501373063 1106 62-63 0.04795196790640688 1106 64-65 0.09208053580438502 1106 66-67 0.0456328674621318 1106 68-69 0.13512940666156226 1106 70-71 0.08784790138418686 1106 72-73 0.15425352408594506 1106 74-75 0.3493959722044764 1106 76-77 0.3670890009198473 1106 78-79 0.4161712036950469 1106 80-81 0.2930201279009772 1106 82-83 0.42419011753140623 1106 84-85 0.39865816034960133 1106 86-87 0.371501631219072 1106 88-89 0.5203486302934088 1106 90-91 0.3928123148493263 1106 92-93 0.5119399534447275 1106 94-95 0.5578222946278579 1106 96-97 0.5716634406524541 1106 98-99 0.5607955698807139 1106 100-101 0.7994868768257142 1107 1 0.056721327998769056 1107 2 0.07978669302206498 1107 3 0.14296123532204064 1107 4 -0.014175640857224892 1107 5 0.04494229643913883 1107 6 0.1507354480911971 1107 7 0.15939033112965006 1107 8 0.0719032694168007 1107 9 0.014184069762741558 1107 10-11 0.09265148833910786 1107 12-13 0.04909060868676107 1107 14-15 0.05596766184460478 1107 16-17 0.037088604071747966 1107 18-19 0.10449569946076309 1107 20-21 0.10459262631211885 1107 22-23 0.0842620764581099 1107 24-25 0.1479485008485284 1107 26-27 0.06893776059166612 1107 28-29 0.18184022090017748 1107 30-31 0.06373167353529396 1107 32-33 0.1581281024857759 1107 34-35 -0.048295352842565364 1107 36-37 0.04035500521818847 1107 38-39 0.1208247287151778 1107 40-41 -0.027520950495478758 1107 42-43 0.16178540814018305 1107 44-45 0.21201374234161108 1107 46-47 0.19847079187204741 1107 48-49 0.14256849842134756 1107 50-51 0.12654876342953258 1107 52-53 0.012297020832761518 1107 54-55 0.17006674452478876 1107 56-57 0.18761816825860222 1107 58-59 0.203298639632429 1107 60-61 0.1423057451661549 1107 62-63 0.038811138383060495 1107 64-65 -0.026629488078221186 1107 66-67 0.03817069302355236 1107 68-69 0.20888590979839705 1107 70-71 0.20665517848559745 1107 72-73 0.20696584414828578 1107 74-75 0.33199481583861257 1107 76-77 0.2918695512593743 1107 78-79 0.34962239830155184 1107 80-81 0.38275304567268975 1107 82-83 0.3283170852702817 1107 84-85 0.41870149052514805 1107 86-87 0.39442010249884873 1107 88-89 0.29800334357155833 1107 90-91 0.27173169894710014 1107 92-93 0.2701314245986204 1107 94-95 0.3109261267529284 1107 96-97 0.36191620991097295 1107 98-99 0.3319954144394437 1107 100-101 0.5883288854636852 1108 1 0.25548801921138775 1108 2 0.3333994020569655 1108 3 0.28862997844345273 1108 4 0.15209142639719175 1108 5 0.18322961999227516 1108 6 0.12509582840856837 1108 7 0.23768646441808983 1108 8 0.2708456273200497 1108 9 0.2749049503517682 1108 10-11 0.2995102199619666 1108 12-13 0.32864791711834584 1108 14-15 0.4190492015160956 1108 16-17 0.4593983177831049 1108 18-19 0.44005935666932317 1108 20-21 0.41327779057976244 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-1.1850387324931262 2316 66-67 -0.9001573407683665 2316 68-69 -0.8888655822227278 2316 70-71 -0.8339861293748996 2316 72-73 -0.844369044322022 2316 74-75 -0.5896517655974165 2316 76-77 -0.9773476995925705 2316 78-79 -0.9222035040986647 2316 80-81 -0.9775774467435525 2316 82-83 -1.211898464347538 2316 84-85 -1.1396021658455773 2316 86-87 -0.9881779272855766 2316 88-89 -1.1081884971681504 2316 90-91 -0.7697047747555672 2316 92-93 -1.039563322285705 2316 94-95 -0.8363467093745527 2316 96-97 -0.8240511777310857 2316 98-99 -0.6953523230724521 2316 100-101 -0.9953364317595437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 41.0 4 82.0 5 206.0 6 326.0 7 901.0 8 2143.0 9 3720.0 10 5820.0 11 8791.0 12 12188.0 13 15392.0 14 18007.0 15 21748.0 16 24950.0 17 28228.0 18 26092.0 19 24485.0 20 25472.0 21 27660.0 22 31040.0 23 36102.0 24 45280.0 25 56856.0 26 73816.0 27 94310.0 28 119713.0 29 148801.0 30 183563.0 31 221790.0 32 266666.0 33 322449.0 34 398766.0 35 495146.0 36 640889.0 37 816766.0 38 743793.0 39 156573.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.63544897380275 21.991216186260164 14.54359126468007 23.829743575257016 2 14.908569893084733 21.94975708208213 40.49462285448658 22.647050170346557 3 17.899300900652104 26.934458283816333 31.178206882646126 23.98803393288544 4 12.565681291154734 18.02478471173219 39.5075234888913 29.902010508221778 5 12.48652305246953 38.6120014019752 36.29742821496665 12.604047330588616 6 29.731615785239278 39.39037215416196 18.08424924086796 12.793762819730803 7 25.511631847876316 33.19246426657734 24.117441927059023 17.178461958487333 8 24.110621236825025 37.4353129602378 21.27858169332013 17.175484109617038 9 25.139808342978164 16.29129796157249 21.683282054420175 36.88561164102917 10-11 23.73699345779655 27.12242095262453 29.845733760013555 19.294851829565363 12-13 24.02992750149649 24.677812759140373 29.875849941199405 21.416409798163727 14-15 21.336662884245055 26.544411190123224 26.878601918120232 25.240324007511493 16-17 21.25998413678454 29.510189880021688 27.77110054783795 21.458725435355827 18-19 21.242459677071377 28.60665541126288 29.37985967879734 20.771025232868396 20-21 21.790552600053193 27.802158166143254 29.27898861410257 21.128300619700983 22-23 21.613922610669942 27.73607338821916 29.03566951137806 21.614334489732837 24-25 21.502208466704325 27.94135991338731 29.04454394966422 21.51188767024415 26-27 21.539123876994832 28.004524873565384 28.902242528908097 21.554108720531687 28-29 21.53732004573032 28.13497151269558 28.874470515066232 21.453237926507875 30-31 21.801926133593632 27.822282748502943 28.814437989390267 21.561353128513158 32-33 21.653893183772382 28.014918951961672 28.69041899521894 21.640768869047 34-35 21.83675304872218 27.933118533078577 28.78531995779843 21.444808460400814 36-37 21.86099632081562 27.803040414345375 28.752585172034745 21.583378092804256 38-39 21.72397950202533 27.892936279463633 28.832153831896406 21.550930386614635 40-41 21.70710507762765 27.82785867181618 28.885979303413073 21.5790569471431 42-43 21.746042145067047 27.903507407486593 28.584283366937008 21.76616708050935 44-45 21.71755356474723 28.034871455885245 28.54237883233191 21.70519614703562 46-47 21.93515953065299 27.948304000861484 28.366079762237163 21.750456706248364 48-49 21.850240968333843 27.960663203072766 28.305204914049447 21.883890914543947 50-51 21.80615796456273 28.058460907790494 28.17534970837738 21.9600314192694 52-53 21.897567646645 27.984289027574754 28.245801697786604 21.872341627993638 54-55 21.740070340330718 27.97317466920261 28.314096834332013 21.97265815613466 56-57 21.812150968119056 28.094097131637124 28.136947016788838 21.956804883454982 58-59 21.791900648382455 27.973853619148798 28.259570343017987 21.974675389450756 60-61 21.8124247529542 28.07056517093283 28.250942213212472 21.866067862900497 62-63 21.77511551679963 27.996059618154028 28.228261392405685 22.000563472640657 64-65 21.55432608659585 27.949484461088097 28.192201883092157 22.303987569223892 66-67 21.58213904128666 27.96163630814065 28.185298846511163 22.270925804061523 68-69 21.71604733562136 27.943001771733183 28.205536757008666 22.135414135636793 70-71 21.654391710126937 27.716651944048227 28.26214630745637 22.366810038368463 72-73 21.72716692504898 27.796591473275754 28.158545885783866 22.3176957158914 74-75 21.596542336936736 27.974911809005874 28.083347186780284 22.345198667277103 76-77 21.548370813378067 27.881999850689 28.115654845294426 22.453974490638508 78-79 21.494279364718206 27.927890377210822 28.080698911971062 22.497131346099906 80-81 21.576408979023448 27.903109707661983 28.06051720587226 22.459964107442314 82-83 21.59664597335576 27.810062315292 28.168691017983804 22.424600693368436 84-85 21.700961295652963 27.78182192617073 27.90314964683846 22.614067131337844 86-87 21.476142022065716 27.88087603862089 28.044264412029367 22.598717527284023 88-89 21.532008461569163 27.706039415080447 27.9212463309648 22.840705792385588 90-91 21.480299160890187 27.68133637750919 28.057317594630327 22.7810468669703 92-93 21.531928191399363 27.581116300119202 28.04865668576278 22.838298822718656 94-95 21.291234358907673 27.496626955056865 28.17681600640501 23.035322679630458 96-97 21.336328803190217 27.330409909567123 28.140222351756993 23.193038935485667 98-99 21.23666395480474 27.386876523461005 28.04296012935346 23.333499392380794 100-101 21.18567925080434 27.279895924126446 28.06096511426301 23.473459710806207 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 197.0 1 144.0 2 132.0 3 195.0 4 185.0 5 184.0 6 300.5 7 539.5 8 863.5 9 1016.0 10 934.0 11 903.5 12 1012.0 13 1167.5 14 1379.0 15 1678.0 16 1951.0 17 2283.5 18 2671.0 19 3064.0 20 3590.5 21 4370.0 22 5408.5 23 7024.5 24 9976.0 25 14894.5 26 22617.0 27 33847.5 28 49196.5 29 71188.5 30 96226.5 31 120618.5 32 143245.5 33 157758.0 34 167216.0 35 174164.5 36 182571.5 37 190682.5 38 186796.5 39 175554.0 40 165515.0 41 158920.0 42 155394.5 43 155244.0 44 156472.5 45 159134.5 46 162553.5 47 164429.5 48 164989.5 49 163225.0 50 159634.5 51 156325.0 52 154124.0 53 152145.5 54 147723.5 55 136883.0 56 123654.0 57 110136.5 58 97440.0 59 85318.0 60 71237.5 61 58493.5 62 48161.5 63 37706.0 64 28161.5 65 21034.5 66 15501.5 67 12177.5 68 9653.5 69 7303.0 70 5596.5 71 4259.0 72 3250.5 73 2576.5 74 2021.0 75 1540.0 76 1135.0 77 711.0 78 432.0 79 289.0 80 167.5 81 100.5 82 65.5 83 38.5 84 22.0 85 17.5 86 10.0 87 5.0 88 4.0 89 2.5 90 1.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02020167152160559 2 0.0030988971848676535 3 0.003353869738052967 4 0.035637317625978014 5 0.0013140893125704607 6 3.922654664389435E-5 7 0.0 8 1.9613273321947174E-5 9 1.9613273321947174E-5 10-11 0.0 12-13 0.0 14-15 4.903318330486794E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.84530932877887E-5 24-25 5.491716530145208E-4 26-27 0.002020167152160559 28-29 1.9613273321947174E-5 30-31 0.006737159386088854 32-33 0.02312404924657572 34-35 0.013209539582331422 36-37 7.747242962169134E-4 38-39 6.962712029291247E-4 40-41 0.0022457197953629515 42-43 0.008531773895047022 44-45 0.008541580531707994 46-47 0.007815889418795949 48-49 0.01003218930417598 50-51 0.19595621375957423 52-53 0.05215169376305754 54-55 0.01850512337925716 56-57 0.010944206513646523 58-59 0.02072142326463719 60-61 0.056348934253954236 62-63 0.10145946289443274 64-65 0.10635297458825857 66-67 0.11076596108569668 68-69 0.10890270012011169 70-71 0.09711512285362144 72-73 0.07350074177399704 74-75 0.2473135699530929 76-77 0.298876865516492 78-79 0.0074138173156960314 80-81 7.453043862339926E-4 82-83 0.0 84-85 9.806636660973587E-6 86-87 0.0 88-89 1.2748627659265663E-4 90-91 0.006433153649598673 92-93 0.01870125611247663 94-95 0.0015494485924338268 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5098588.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.5851895082006 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13866581094786 36.9973327042375 2 14.167684032214975 14.333499633238045 3 4.976159757039375 7.551599529987548 4 2.3918003642679326 4.839586987691065 5 1.3553863146079121 3.428123679063143 6 0.8379863752296013 2.543381975776769 7 0.5698992462734248 2.017992296132532 8 0.4181409623224599 1.6921391856178403 9 0.2980550556440614 1.3569454326268908 >10 1.6845211824287953 15.278432853196723 >50 0.10951332750970799 3.829277157785654 >100 0.048540826694619066 4.351659719041384 >500 0.0025216870461021532 0.8397174473462138 >1k 0.0010862626776604958 0.8403438252099055 >5k 3.879509562953231E-5 0.09996757304897698 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.9613273321947174E-5 0.0 10-11 0.0 0.0 0.0 5.883981996584152E-5 9.806636660973587E-6 12-13 0.0 9.806636660973587E-6 0.0 7.84530932877887E-5 1.3729291325363023E-4 14-15 0.0 1.9613273321947174E-5 0.0 9.806636660973587E-5 2.451659165243397E-4 16-17 0.0 1.9613273321947174E-5 0.0 1.2748627659265663E-4 3.530389197950491E-4 18-19 0.0 1.9613273321947174E-5 0.0 1.8632609655849815E-4 4.0207210309991707E-4 20-21 0.0 1.9613273321947174E-5 0.0 2.3535927986336608E-4 4.707185597267322E-4 22-23 0.0 1.9613273321947174E-5 0.0 2.7458582650726046E-4 5.197517430316002E-4 24-25 0.0 1.9613273321947174E-5 0.0 3.4323228313407555E-4 5.295583796925737E-4 26-27 0.0 1.9613273321947174E-5 0.0 6.17818109641336E-4 5.687849263364681E-4 28-29 0.0 1.9613273321947174E-5 0.0 0.001588675139077721 6.374313829632832E-4 30-31 0.0 1.9613273321947174E-5 0.0 0.005854562086601232 6.472380196242567E-4 32-33 0.0 1.9613273321947174E-5 0.0 0.020005538788386117 6.66851292946204E-4 34-35 0.0 3.922654664389435E-5 0.0 0.04337475395148618 6.864645662681511E-4 36-37 0.0 4.903318330486794E-5 0.0 0.07987505560362987 6.864645662681511E-4 38-39 0.0 5.8839819965841526E-5 0.0 0.1319482962733996 7.256911129120455E-4 40-41 0.0 5.8839819965841526E-5 0.0 0.18764999250772957 7.256911129120455E-4 42-43 9.806636660973587E-6 5.8839819965841526E-5 0.0 0.24728415004310997 7.256911129120455E-4 44-45 1.9613273321947174E-5 5.8839819965841526E-5 0.0 0.32189304175979705 7.649176595559398E-4 46-47 1.9613273321947174E-5 5.8839819965841526E-5 0.0 0.41242791141390517 8.139508428608078E-4 48-49 1.9613273321947174E-5 5.8839819965841526E-5 0.0 0.5059047720663055 8.629840261656757E-4 50-51 1.9613273321947174E-5 5.8839819965841526E-5 0.0 0.6032454475631293 9.218238461315172E-4 52-53 1.9613273321947174E-5 5.8839819965841526E-5 0.0 0.7030672021351794 0.001019890212741253 54-55 1.9613273321947174E-5 5.8839819965841526E-5 0.0 0.8155493246365464 0.0010296968494022266 56-57 1.9613273321947174E-5 5.8839819965841526E-5 0.0 0.947870273103063 0.0010591167593851473 58-59 1.9613273321947174E-5 5.8839819965841526E-5 0.0 1.0920768652026795 0.0010787300327070946 60-61 1.9613273321947174E-5 5.8839819965841526E-5 0.0 1.2446681316474286 0.0010787300327070946 62-63 1.9613273321947174E-5 5.8839819965841526E-5 0.0 1.4010349532066524 0.0011179565793509889 64-65 1.9613273321947174E-5 5.8839819965841526E-5 0.0 1.5678164228998304 0.0011571831259948834 66-67 1.9613273321947174E-5 5.8839819965841526E-5 0.0 1.7582220803092934 0.0012062163092997511 68-69 1.9613273321947174E-5 5.8839819965841526E-5 0.0 1.9732522023744612 0.0012258295826216984 70-71 1.9613273321947174E-5 5.8839819965841526E-5 0.0 2.2092489136207907 0.0012454428559436456 72-73 1.9613273321947174E-5 5.8839819965841526E-5 0.0 2.454120631045301 0.0014121556791801965 74-75 1.9613273321947174E-5 5.8839819965841526E-5 0.0 2.7073377962683 0.0014906087724679852 76-77 1.9613273321947174E-5 5.8839819965841526E-5 0.0 2.9916910328898902 0.0015298353191118795 78-79 1.9613273321947174E-5 5.8839819965841526E-5 0.0 3.3118679132340167 0.0015494485924338268 80-81 1.9613273321947174E-5 5.8839819965841526E-5 0.0 3.663416224256598 0.001569061865755774 82-83 1.9613273321947174E-5 6.864645662681512E-5 0.0 4.030978772946549 0.0017063547790094043 84-85 1.9613273321947174E-5 8.825972994876228E-5 0.0 4.412623651881658 0.0017063547790094043 86-87 1.9613273321947174E-5 9.806636660973587E-5 0.0 4.826208746421559 0.0017750012356362193 88-89 1.9613273321947174E-5 9.806636660973587E-5 0.0 5.276804087719973 0.0018436476922630343 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4440 0.0 49.299763 1 GTATCAA 8630 0.0 38.29352 1 ATCAACG 9765 0.0 33.006927 3 CAACGCA 9805 0.0 32.762913 5 TCAACGC 9870 0.0 32.605778 4 AACGCAG 10350 0.0 31.174948 6 TATCAAC 10840 0.0 29.997557 2 ACGCAGA 12195 0.0 26.419535 7 CGCAGAG 12655 0.0 25.534191 8 GTACATG 12880 0.0 25.10488 1 ACATGGG 12625 0.0 24.288946 3 TACATGG 13330 0.0 23.788712 2 GCAGAGT 14900 0.0 21.432161 9 CATGGGG 9340 0.0 20.17556 4 GAGTACA 9600 0.0 19.685148 1 AGAGTAC 13835 0.0 19.052065 10-11 TCTAACG 445 0.0 18.13483 2 CTAACGC 425 3.6379788E-12 17.870579 3 CAGAGTA 14840 0.0 17.745827 10-11 GAGTACT 9285 0.0 17.119854 12-13 >>END_MODULE