FastQCFastQC Report
Sat 18 Jun 2016
SRR1930925_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930925_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4332303
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77220.17824238055371475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31550.053.5553971
GTATCAA59900.044.7121431
ATCAACG74100.035.7851333
TCAACGC75050.035.7235344
CAACGCA74500.034.152095
TATCAAC78400.033.6394582
AACGCAG81750.031.5631396
ACGCAGA93400.027.0138347
GTACATG78850.026.7382141
CGCAGAG98050.026.2268188
TACATGG83850.024.536942
ACATGGG83650.024.1062243
GCAGAGT108400.022.2850349
CATGGGG64200.019.7029914
AGAGTAC97800.019.21738610-11
CAGAGTA105800.018.4121510-11
GAGTACT70150.018.11335412-13
GAGTACA60500.017.674141
GTGGTAT15800.017.557531
GTACTTT76250.016.56276714-15
Produced by FastQC (version 0.11.5)