FastQCFastQC Report
Sat 18 Jun 2016
SRR1930925_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930925_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4332303
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55790.1287767730004111No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48960.1130114860387189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44950.052.8208581
GTATCAA80450.039.547031
TCAACGC98800.031.1862134
ATCAACG99250.030.9020213
CAACGCA99250.030.8891835
AACGCAG104800.029.4790946
TATCAAC106000.029.1595042
ACGCAGA124450.024.6338337
GTACATG111550.024.3921571
CGCAGAG129900.023.5637788
ACATGGG113150.022.867933
TACATGG116200.022.8407652
GCAGAGT149600.020.1435749
CATGGGG80450.019.7103944
GAGTACA85550.019.7048661
AGAGTAC135250.019.1404410-11
GAGTACT95350.018.66401112-13
CAGAGTA145300.018.24113810-11
TATAGCG2755.971433E-717.2572565
GTACTTT102300.017.16407814-15
Produced by FastQC (version 0.11.5)