FastQCFastQC Report
Sat 18 Jun 2016
SRR1930924_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930924_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746397
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN67930.18132087976794772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.055.175331
GTATCAA64850.046.689331
TCAACGC83250.036.763864
ATCAACG82150.036.5327533
TATCAAC84450.035.1889232
CAACGCA86800.033.891835
AACGCAG93850.032.1110536
GTACATG71450.027.6860031
ACGCAGA109250.027.3454137
TACATGG73000.026.5398642
GTGGTAT16450.026.3820761
CGCAGAG113350.026.2328158
ACATGGG77200.024.8278623
GCAGAGT121200.022.6130149
CATGGGG59700.020.4389064
TGGTATC18250.019.9048982
AGAGTAC109200.019.80490710-11
CAGAGTA116200.019.06810610-11
GAGTACT84250.018.59066812-13
AGTACTT88300.017.97171612-13
Produced by FastQC (version 0.11.5)