##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930924_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3746397 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.453362524046437 30.0 10.0 31.0 2.0 33.0 2 21.36382182667774 28.0 10.0 31.0 2.0 33.0 3 21.80350774357336 28.0 10.0 31.0 2.0 33.0 4 23.713556251513122 32.0 2.0 35.0 2.0 37.0 5 23.710187147811617 32.0 10.0 35.0 2.0 37.0 6 24.424065842461438 32.0 10.0 35.0 2.0 37.0 7 24.483173833419148 32.0 10.0 35.0 2.0 37.0 8 25.225754771851463 32.0 17.0 35.0 2.0 37.0 9 26.319737070043566 33.0 17.0 37.0 2.0 39.0 10-11 26.538099939755448 33.0 17.0 37.0 2.0 39.0 12-13 26.4604581415157 33.0 17.0 37.0 2.0 39.0 14-15 26.662944290207363 33.0 14.0 38.0 2.0 40.0 16-17 25.801379832409644 32.5 10.0 38.0 2.0 40.0 18-19 25.21107520105317 32.0 10.0 38.0 2.0 40.0 20-21 24.68704491275217 32.0 8.0 38.0 2.0 40.0 22-23 24.604266445867857 31.5 10.0 38.0 2.0 40.0 24-25 24.29230231072681 31.0 5.0 37.0 2.0 39.5 26-27 23.931969970080587 30.5 4.5 37.0 2.0 39.0 28-29 23.800606422650883 30.0 2.0 37.0 2.0 39.0 30-31 23.666662395896644 30.0 2.0 37.0 2.0 39.0 32-33 23.31496595262061 30.0 2.0 37.0 2.0 39.0 34-35 20.46716231622009 24.5 2.0 35.5 2.0 39.0 36-37 18.847715551768808 17.0 2.0 34.0 2.0 38.5 38-39 17.925857830870566 15.5 2.0 33.0 2.0 38.0 40-41 19.13385407366064 20.0 2.0 34.0 2.0 38.0 42-43 19.012394174989996 20.0 2.0 34.0 2.0 38.0 44-45 17.13224132413089 12.5 2.0 33.0 2.0 38.0 46-47 17.825227144907494 17.5 2.0 32.5 2.0 38.0 48-49 17.24710555234803 14.0 2.0 33.0 2.0 38.0 50-51 15.405174358190015 9.5 2.0 30.5 2.0 37.0 52-53 18.082631525703228 18.5 2.0 32.5 2.0 37.5 54-55 19.614678850105847 24.5 2.0 34.5 2.0 38.0 56-57 19.80537487084257 25.0 2.0 35.0 2.0 39.0 58-59 19.44092377289433 23.5 2.0 35.0 2.0 39.0 60-61 19.107464585306897 23.0 2.0 34.5 2.0 38.0 62-63 18.62133591287843 22.0 2.0 34.0 2.0 38.0 64-65 18.119327182890657 20.5 2.0 34.0 2.0 38.0 66-67 17.697144883470706 18.5 2.0 33.5 2.0 37.0 68-69 17.23960487903444 16.0 2.0 33.0 2.0 36.5 70-71 16.659996124276205 10.0 2.0 32.0 2.0 36.0 72-73 16.179384619408996 7.5 2.0 32.0 2.0 35.5 74-75 15.657789871174892 4.5 2.0 31.0 2.0 35.0 76-77 15.0966361813764 2.0 2.0 31.0 2.0 35.0 78-79 14.601404629568089 2.0 2.0 30.5 2.0 34.0 80-81 14.097870567374468 2.0 2.0 30.0 2.0 34.0 82-83 13.632300180680264 2.0 2.0 29.5 2.0 34.0 84-85 13.122262536511748 2.0 2.0 29.0 2.0 34.0 86-87 12.575909200226244 2.0 2.0 29.0 2.0 34.0 88-89 12.041710475424788 2.0 2.0 27.5 2.0 34.0 90-91 11.480612038713463 2.0 2.0 26.5 2.0 33.0 92-93 10.87789441428658 2.0 2.0 25.5 2.0 33.0 94-95 10.24910213733355 2.0 2.0 24.5 2.0 32.0 96-97 9.554897011715523 2.0 2.0 20.5 2.0 32.0 98-99 8.80584972708445 2.0 2.0 8.5 2.0 32.0 100-101 7.8465947415610255 2.0 2.0 2.0 2.0 31.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.09276092326602 1101 2 8.108555286419396 1101 3 7.565977373737137 1101 4 9.480093792048553 1101 5 9.29016609775412 1101 6 8.6811608782081 1101 7 8.473709317396558 1101 8 7.560301058447465 1101 9 8.01845121110637 1101 10-11 7.456533972822328 1101 12-13 7.081514068818873 1101 14-15 7.707298420870146 1101 16-17 8.555241159459072 1101 18-19 8.825991791983402 1101 20-21 9.40210247999498 1101 22-23 9.050859820065341 1101 24-25 9.114927601139474 1101 26-27 9.129302261363474 1101 28-29 8.92270857201866 1101 30-31 8.677311500450035 1101 32-33 8.714665897517587 1101 34-35 11.286424366051381 1101 36-37 5.3175759921568435 1101 38-39 8.263234990668774 1101 40-41 10.025204866086817 1101 42-43 10.354951704458006 1101 44-45 4.693963921958456 1101 46-47 6.116068023172364 1101 48-49 9.235540729572442 1101 50-51 -3.303084924787381 1101 52-53 5.930683649087111 1101 54-55 8.52786677073206 1101 56-57 9.099919320280613 1101 58-59 9.103952649135941 1101 60-61 8.891756157275708 1101 62-63 9.134666437918828 1101 64-65 9.069420040104536 1101 66-67 9.058176828977743 1101 68-69 9.102873186992635 1101 70-71 9.128330398766156 1101 72-73 8.902139577003535 1101 74-75 8.910274032067626 1101 76-77 8.681882999970814 1101 78-79 8.668458294399633 1101 80-81 8.502837386876768 1101 82-83 8.28200034978809 1101 84-85 8.115029246942928 1101 86-87 7.910602963404013 1101 88-89 7.884228254327439 1101 90-91 7.805824829139672 1101 92-93 7.61390915231109 1101 94-95 7.356165335325647 1101 96-97 7.038480413726678 1101 98-99 6.724462099807109 1101 100-101 6.179204498969528 1102 1 6.625206195648566 1102 2 7.638602650412622 1102 3 7.432541384278871 1102 4 9.149582013082654 1102 5 9.189657548250441 1102 6 8.52694011054329 1102 7 8.260258570908473 1102 8 7.406485462629121 1102 9 7.510153033101265 1102 10-11 6.99481109031737 1102 12-13 6.748156535099472 1102 14-15 7.149597933080429 1102 16-17 7.788660456825134 1102 18-19 8.205099786333367 1102 20-21 8.545932456581014 1102 22-23 8.229084150453126 1102 24-25 8.57334616849889 1102 26-27 8.471380251060285 1102 28-29 8.420490622978495 1102 30-31 8.115631931672588 1102 32-33 8.27516830334346 1102 34-35 10.644713551536846 1102 36-37 1.4091476986392557 1102 38-39 3.78640994137335 1102 40-41 8.651904165382131 1102 42-43 9.70163193205737 1102 44-45 1.5358304639598401 1102 46-47 2.1222521963668406 1102 48-49 7.8649064053719115 1102 50-51 -4.173359984026876 1102 52-53 5.065942766579536 1102 54-55 7.904403216804035 1102 56-57 8.369557879100775 1102 58-59 8.674350313172791 1102 60-61 8.56009398226849 1102 62-63 8.374625904753938 1102 64-65 8.268524521706116 1102 66-67 8.198579442532274 1102 68-69 8.056379100191723 1102 70-71 8.115973238548658 1102 72-73 8.08464686726391 1102 74-75 7.926372458173304 1102 76-77 7.796631799963375 1102 78-79 7.796200014126763 1102 80-81 7.472573962708333 1102 82-83 7.435163878975791 1102 84-85 7.457396006929379 1102 86-87 7.201649529983015 1102 88-89 7.034453125428605 1102 90-91 7.005613156268071 1102 92-93 6.8721817619243115 1102 94-95 6.470836013711741 1102 96-97 6.225528526902842 1102 98-99 5.836650428736917 1102 100-101 5.409811600096439 1103 1 6.621819422717454 1103 2 7.455707019618213 1103 3 7.2240622982906615 1103 4 9.043728153076568 1103 5 8.962823396605973 1103 6 8.204352426652928 1103 7 8.108029875762906 1103 8 7.3129395862707085 1103 9 7.342619699744379 1103 10-11 6.72971142146929 1103 12-13 6.749540427189093 1103 14-15 7.080820686711249 1103 16-17 7.521521905449596 1103 18-19 8.063246112871841 1103 20-21 8.3800990458152 1103 22-23 8.339599857822662 1103 24-25 8.279799479541087 1103 26-27 8.376958286569465 1103 28-29 8.364486273098638 1103 30-31 8.049779689799198 1103 32-33 8.021039185540221 1103 34-35 10.182664168812071 1103 36-37 -2.872081608782283 1103 38-39 -1.7518774453529424 1103 40-41 6.295074638488092 1103 42-43 8.853127556562963 1103 44-45 0.8434511270197476 1103 46-47 0.4998365009036547 1103 48-49 7.043459434371464 1103 50-51 -4.791871316078657 1103 52-53 4.269027923815752 1103 54-55 7.27619969386771 1103 56-57 7.632519810939229 1103 58-59 7.815949855008057 1103 60-61 7.94799967692968 1103 62-63 7.845183344401651 1103 64-65 7.950177511382925 1103 66-67 7.60981850467952 1103 68-69 7.503739194424988 1103 70-71 7.444011167861696 1103 72-73 7.444203866341432 1103 74-75 7.399218283691255 1103 76-77 7.266822227330593 1103 78-79 7.056845083785964 1103 80-81 7.031952967189289 1103 82-83 6.757132468123066 1103 84-85 6.688561701950938 1103 86-87 6.35980447714109 1103 88-89 5.991360061247029 1103 90-91 5.717877860165688 1103 92-93 5.661266785611582 1103 94-95 5.331787609992579 1103 96-97 5.154160537029949 1103 98-99 4.86695964400262 1103 100-101 4.417496069873573 1104 1 6.380819781508652 1104 2 7.447274067364624 1104 3 6.913637579207862 1104 4 8.706357195593323 1104 5 8.656682494952815 1104 6 7.8595456574589555 1104 7 7.730089622652027 1104 8 6.884239755881097 1104 9 6.866176380599601 1104 10-11 6.36560117607786 1104 12-13 6.147861685295876 1104 14-15 6.601990692582376 1104 16-17 7.277912262120665 1104 18-19 7.62049158856917 1104 20-21 7.87006390058631 1104 22-23 7.807357270122679 1104 24-25 7.805148825290775 1104 26-27 7.775973071756823 1104 28-29 7.679204764707819 1104 30-31 7.253850644836138 1104 32-33 7.208684876512272 1104 34-35 9.384298673381224 1104 36-37 -3.2299312195481935 1104 38-39 -2.1661377307006724 1104 40-41 5.560805220273462 1104 42-43 7.574522486462772 1104 44-45 0.08777002852068705 1104 46-47 -0.5402778162242754 1104 48-49 6.130974010263959 1104 50-51 -5.051956576555632 1104 52-53 3.4330669206138076 1104 54-55 6.069374031055332 1104 56-57 6.5039279916503965 1104 58-59 6.760413258305675 1104 60-61 6.607616096519678 1104 62-63 6.702982534131525 1104 64-65 6.643708635160454 1104 66-67 6.478168466082284 1104 68-69 6.377450264220748 1104 70-71 6.483835394150216 1104 72-73 6.427969481912971 1104 74-75 6.25050242321754 1104 76-77 6.054179798096719 1104 78-79 5.83746240850831 1104 80-81 5.683882896898831 1104 82-83 5.805341434097704 1104 84-85 5.428851439435377 1104 86-87 5.125226281045393 1104 88-89 5.1220080327176944 1104 90-91 4.8971935135190225 1104 92-93 4.520158453773211 1104 94-95 4.276551297061419 1104 96-97 4.240434769026514 1104 98-99 3.86378988004374 1104 100-101 3.35200984664021 1105 1 5.965327655896569 1105 2 6.741092089913291 1105 3 6.651066208705149 1105 4 8.51893872812126 1105 5 8.4131381381066 1105 6 7.579018618594333 1105 7 7.325680012684732 1105 8 6.534130809517684 1105 9 6.54892976951685 1105 10-11 6.158869937224573 1105 12-13 5.882345493802358 1105 14-15 6.09381794288015 1105 16-17 6.746103941548945 1105 18-19 7.206706131766442 1105 20-21 7.586347246208604 1105 22-23 7.448623009445463 1105 24-25 7.452328430027382 1105 26-27 7.461260861886583 1105 28-29 7.107993191342725 1105 30-31 7.063140744660313 1105 32-33 6.717337171396917 1105 34-35 9.066482713172249 1105 36-37 -3.604183050288242 1105 38-39 -2.4054347154938718 1105 40-41 5.05055555743213 1105 42-43 7.301178384533724 1105 44-45 -3.239029083208022 1105 46-47 -4.179146420450419 1105 48-49 4.679543173480262 1105 50-51 -9.204299711122903 1105 52-53 0.37215345695447155 1105 54-55 5.027859007013085 1105 56-57 6.097020611026622 1105 58-59 6.467594501570378 1105 60-61 6.4374045373341495 1105 62-63 6.2948143534018755 1105 64-65 6.210407166472091 1105 66-67 6.187549709031888 1105 68-69 6.169994488724306 1105 70-71 6.3215523809600676 1105 72-73 6.159958473840071 1105 74-75 6.0258777485599 1105 76-77 5.852744431728141 1105 78-79 5.600525337440304 1105 80-81 5.5041154891133015 1105 82-83 5.335545887743114 1105 84-85 5.151376553029486 1105 86-87 5.051469474851757 1105 88-89 4.918754136486939 1105 90-91 4.636896979321053 1105 92-93 4.483529276314316 1105 94-95 4.405167087430655 1105 96-97 4.188059966752741 1105 98-99 3.892648653101297 1105 100-101 3.4639144228601273 1106 1 5.7347941216216896 1106 2 6.877554286897851 1106 3 6.490138339319184 1106 4 7.826658604251239 1106 5 7.625741162469495 1106 6 6.903945762076429 1106 7 6.801202174296687 1106 8 6.1252877593628945 1106 9 5.946231733867467 1106 10-11 5.483060468234406 1106 12-13 5.4432031172883875 1106 14-15 5.678803696002873 1106 16-17 6.091777704027322 1106 18-19 6.436656775879403 1106 20-21 6.874249110291217 1106 22-23 6.667975304989717 1106 24-25 6.72356124493761 1106 26-27 6.745718674553707 1106 28-29 6.681596586768212 1106 30-31 6.164491207195919 1106 32-33 6.233684518933025 1106 34-35 8.545971873531613 1106 36-37 -0.2640226975151805 1106 38-39 0.4194367305806459 1106 40-41 5.68913900824327 1106 42-43 6.7716864488086586 1106 44-45 -3.675270759011852 1106 46-47 -4.597396376221987 1106 48-49 3.555511890166372 1106 50-51 -9.268592261534444 1106 52-53 -0.3669765508064131 1106 54-55 3.9751020930575294 1106 56-57 4.778987273181734 1106 58-59 5.290395512158334 1106 60-61 5.338090736999284 1106 62-63 5.311693139114748 1106 64-65 5.259030369644577 1106 66-67 5.074575279908142 1106 68-69 4.838194703352585 1106 70-71 4.948329945664106 1106 72-73 4.724233979197411 1106 74-75 4.622348446527624 1106 76-77 4.397349881177703 1106 78-79 4.322734485451187 1106 80-81 4.307550403365907 1106 82-83 4.050274791542092 1106 84-85 3.869098669728672 1106 86-87 3.8536516093031423 1106 88-89 3.6568571955735862 1106 90-91 3.6134476103555713 1106 92-93 3.56073412060743 1106 94-95 3.2597798085918175 1106 96-97 2.9371939005788974 1106 98-99 2.6113454427508813 1106 100-101 2.3157379014908246 1107 1 5.875992951211295 1107 2 6.970279283245851 1107 3 6.542222733207826 1107 4 7.742135358600315 1107 5 8.324083695889591 1107 6 6.74079263830145 1107 7 6.918701742696786 1107 8 6.1634288942517585 1107 9 6.500930934559211 1107 10-11 5.902410247614313 1107 12-13 5.543363866732715 1107 14-15 5.775318041104754 1107 16-17 6.774785714324722 1107 18-19 6.918521034144849 1107 20-21 7.418600488189266 1107 22-23 7.208396357129445 1107 24-25 7.237850275709331 1107 26-27 7.126465347299661 1107 28-29 7.056982198530942 1107 30-31 6.796196621942517 1107 32-33 6.690977708803043 1107 34-35 8.868817083362945 1107 36-37 -0.08099445981504161 1107 38-39 0.6617899730886379 1107 40-41 6.435547445369618 1107 42-43 7.614688173240854 1107 44-45 -3.140559490737715 1107 46-47 -4.172381817410539 1107 48-49 4.245008162667972 1107 50-51 -9.152848603839644 1107 52-53 0.08232527794753608 1107 54-55 4.744639181875627 1107 56-57 5.889065290704444 1107 58-59 6.2171196861302285 1107 60-61 6.096733371298171 1107 62-63 6.0213295721469215 1107 64-65 6.186953862598553 1107 66-67 5.941709463948239 1107 68-69 5.911734899689964 1107 70-71 5.844255347494681 1107 72-73 5.685811074744038 1107 74-75 5.643048887669448 1107 76-77 5.536319761652813 1107 78-79 5.527631496615731 1107 80-81 5.231767117176565 1107 82-83 4.907546794898492 1107 84-85 4.913934890477634 1107 86-87 4.633795911225349 1107 88-89 4.5241269462490195 1107 90-91 4.51390745484189 1107 92-93 4.440771235739593 1107 94-95 3.9889295371142506 1107 96-97 3.916135022482875 1107 98-99 3.768406859785575 1107 100-101 3.42016503439359 1108 1 6.01598852832084 1108 2 6.440913287581466 1108 3 6.651831524355931 1108 4 8.050477635097039 1108 5 8.08494794446294 1108 6 7.400165850131028 1108 7 7.64852341441625 1108 8 6.595667574524363 1108 9 5.1393936620921465 1108 10-11 6.022673606826729 1108 12-13 5.474450228722876 1108 14-15 6.045970215285763 1108 16-17 6.187724357690602 1108 18-19 6.759885278896853 1108 20-21 7.504141876853602 1108 22-23 7.564884162993643 1108 24-25 7.267936778472649 1108 26-27 7.499214178648241 1108 28-29 7.348145342293865 1108 30-31 7.012344252306228 1108 32-33 7.21761933707759 1108 34-35 9.342848688539352 1108 36-37 -3.4383800951839643 1108 38-39 -2.2880352438462506 1108 40-41 5.127417438030182 1108 42-43 7.561372620222993 1108 44-45 -2.9243160803883974 1108 46-47 -4.048792291722394 1108 48-49 4.809447691967897 1108 50-51 -9.204100696022149 1108 52-53 0.43944933657326857 1108 54-55 5.277269647898876 1108 56-57 6.343946199324915 1108 58-59 6.790492024229387 1108 60-61 6.642620613231511 1108 62-63 6.703178475952999 1108 64-65 6.754561712464845 1108 66-67 6.794339382657288 1108 68-69 6.691864361687497 1108 70-71 6.486720495043933 1108 72-73 6.42287800313537 1108 74-75 6.441639541268618 1108 76-77 6.24474650204418 1108 78-79 6.1479275837382925 1108 80-81 5.9991912469778 1108 82-83 5.869920299368593 1108 84-85 5.56644843095707 1108 86-87 5.465592203837275 1108 88-89 5.5223287602258 1108 90-91 5.259774924433282 1108 92-93 5.013922306047034 1108 94-95 5.0075880206939605 1108 96-97 5.03476377358829 1108 98-99 4.553552012689632 1108 100-101 4.148446522033043 1109 1 4.693424052446257 1109 2 6.342029934342712 1109 3 6.112106881843832 1109 4 7.5383141525502815 1109 5 7.307096798144894 1109 6 5.089080748105161 1109 7 4.670550917279986 1109 8 3.0117631125711206 1109 9 2.4676528570760787 1109 10-11 2.3979077882455755 1109 12-13 3.1503259551212537 1109 14-15 3.7494193712610553 1109 16-17 2.971418268126669 1109 18-19 3.7311369561589878 1109 20-21 4.072724894913971 1109 22-23 5.809765928781335 1109 24-25 5.621124523210916 1109 26-27 4.386670574815437 1109 28-29 4.332827137080191 1109 30-31 4.099665209400701 1109 32-33 3.8335945083663496 1109 34-35 6.190246982801757 1109 36-37 -4.6217848787629645 1109 38-39 -3.5066622830307814 1109 40-41 2.8075345885306078 1109 42-43 3.602234225980073 1109 44-45 -4.589090860145756 1109 46-47 -5.863604615245521 1109 48-49 1.6501077273143423 1109 50-51 -9.532204185275463 1109 52-53 -1.449308510372731 1109 54-55 2.085925304551047 1109 56-57 3.398110368471663 1109 58-59 4.047262607655021 1109 60-61 4.337345826249408 1109 62-63 4.335382295003509 1109 64-65 4.056388676312714 1109 66-67 4.289775211716812 1109 68-69 4.178320578979953 1109 70-71 4.256215413058012 1109 72-73 4.267048143908298 1109 74-75 4.2941745675841325 1109 76-77 4.344018247657392 1109 78-79 4.320257703803604 1109 80-81 4.062740921590189 1109 82-83 3.809727764872923 1109 84-85 3.7446603038553548 1109 86-87 3.7553251298942385 1109 88-89 3.763635771567655 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-12.997992932113604 2313 78-79 -12.503542424269042 2313 80-81 -11.973814976108896 2313 82-83 -11.508360820247995 2313 84-85 -10.997000236592715 2313 86-87 -10.443247865734183 2313 88-89 -9.909940807320703 2313 90-91 -9.348881042882386 2313 92-93 -8.75919847083166 2313 94-95 -8.209559814049692 2313 96-97 -7.507528110776564 2313 98-99 -6.746084227769488 2313 100-101 -5.792849625033156 2314 1 -20.349118490096284 2314 2 -19.28009218777941 2314 3 -19.678183408446458 2314 4 -21.60382798959186 2314 5 -21.583543842495747 2314 6 -22.277285618261207 2314 7 -22.352472105743036 2314 8 -23.073321972513966 2314 9 -24.166231026807804 2314 10-11 -24.38349861816083 2314 12-13 -24.311886257090958 2314 14-15 -24.498703751862067 2314 16-17 -23.659587737879335 2314 18-19 -23.086555736533708 2314 20-21 -22.566664494475418 2314 22-23 -22.460003840988435 2314 24-25 -22.156528129441735 2314 26-27 -21.808774870630007 2314 28-29 -21.68769338344033 2314 30-31 -21.552862318126824 2314 32-33 -21.21132541155357 2314 34-35 -18.439132396577623 2314 36-37 -16.8072203141984 2314 38-39 -15.918323944692442 2314 40-41 -17.11113408013806 2314 42-43 -16.97421722547138 2314 44-45 -15.108860629915526 2314 46-47 -15.776517528158433 2314 48-49 -15.225893273686658 2314 50-51 -13.381303284374562 2314 52-53 -16.03889809996232 2314 54-55 -17.535821442195697 2314 56-57 -17.721509251147957 2314 58-59 -17.348383037990622 2314 60-61 -17.022013533109952 2314 62-63 -16.53711520249399 2314 64-65 -16.045463175539137 2314 66-67 -15.626641204699606 2314 68-69 -15.166274015614643 2314 70-71 -14.576992795150193 2314 72-73 -14.090934839074684 2314 74-75 -13.568813420403856 2314 76-77 -13.036612382973239 2314 78-79 -12.539852406306506 2314 80-81 -12.043740559891292 2314 82-83 -11.583290253145096 2314 84-85 -11.076035391840342 2314 86-87 -10.524079274510164 2314 88-89 -9.989617481685599 2314 90-91 -9.430867186069454 2314 92-93 -8.830278789025142 2314 94-95 -8.209559814049692 2314 96-97 -7.516381074594886 2314 98-99 -6.767254358639388 2314 100-101 -5.816585832372135 2315 1 -20.349118490096284 2315 2 -19.28009218777941 2315 3 -19.678183408446458 2315 4 -17.098875042588393 2315 5 -21.583543842495747 2315 6 -22.277285618261207 2315 7 -22.352472105743036 2315 8 -23.073321972513966 2315 9 -19.994859060487844 2315 10-11 -18.58681709265315 2315 12-13 -15.534149753871542 2315 14-15 -15.420447189207287 2315 16-17 -20.042574364922427 2315 18-19 -23.086555736533708 2315 20-21 -22.566664494475418 2315 22-23 -22.460003840988435 2315 24-25 -22.156528129441735 2315 26-27 -21.808774870630007 2315 28-29 -21.68769338344033 2315 30-31 -21.552862318126824 2315 32-33 -21.21132541155357 2315 34-35 -18.439132396577623 2315 36-37 -16.8072203141984 2315 38-39 -15.918323944692442 2315 40-41 -17.11113408013806 2315 42-43 -16.97421722547138 2315 44-45 -15.108860629915526 2315 46-47 -15.776517528158433 2315 48-49 -15.225893273686658 2315 50-51 -13.381303284374562 2315 52-53 -16.03889809996232 2315 54-55 -17.535821442195697 2315 56-57 -17.721509251147957 2315 58-59 -17.348383037990622 2315 60-61 -17.022013533109952 2315 62-63 -16.53711520249399 2315 64-65 -16.045463175539137 2315 66-67 -15.626641204699606 2315 68-69 -15.166274015614643 2315 70-71 -14.576992795150193 2315 72-73 -14.090934839074684 2315 74-75 -13.568813420403856 2315 76-77 -13.036612382973239 2315 78-79 -12.539852406306506 2315 80-81 -12.043740559891292 2315 82-83 -11.583290253145096 2315 84-85 -11.076035391840342 2315 86-87 -10.524079274510164 2315 88-89 -9.989617481685599 2315 90-91 -9.430867186069454 2315 92-93 -8.830278789025142 2315 94-95 -8.209559814049692 2315 96-97 -7.516381074594886 2315 98-99 -6.767254358639388 2315 100-101 -5.816585832372135 2316 1 -20.349118490096284 2316 2 -19.28009218777941 2316 3 -19.678183408446458 2316 4 -21.60382798959186 2316 5 -21.583543842495747 2316 6 -22.277285618261207 2316 7 -22.352472105743036 2316 8 -23.073321972513966 2316 9 -24.166231026807804 2316 10-11 -24.38349861816083 2316 12-13 -24.311886257090958 2316 14-15 -24.498703751862067 2316 16-17 -23.659587737879335 2316 18-19 -23.086555736533708 2316 20-21 -22.566664494475418 2316 22-23 -22.460003840988435 2316 24-25 -22.156528129441735 2316 26-27 -21.808774870630007 2316 28-29 -21.68769338344033 2316 30-31 -21.552862318126824 2316 32-33 -21.21132541155357 2316 34-35 -18.439132396577623 2316 36-37 -16.8072203141984 2316 38-39 -15.918323944692442 2316 40-41 -17.11113408013806 2316 42-43 -16.97421722547138 2316 44-45 -15.108860629915526 2316 46-47 -15.776517528158433 2316 48-49 -15.225893273686658 2316 50-51 -13.381303284374562 2316 52-53 -16.03889809996232 2316 54-55 -17.535821442195697 2316 56-57 -17.721509251147957 2316 58-59 -17.348383037990622 2316 60-61 -17.022013533109952 2316 62-63 -16.53711520249399 2316 64-65 -16.045463175539137 2316 66-67 -15.626641204699606 2316 68-69 -15.166274015614643 2316 70-71 -14.576992795150193 2316 72-73 -14.090934839074684 2316 74-75 -13.568813420403856 2316 76-77 -13.036612382973239 2316 78-79 -12.539852406306506 2316 80-81 -12.043740559891292 2316 82-83 -11.583290253145096 2316 84-85 -11.076035391840342 2316 86-87 -10.524079274510164 2316 88-89 -9.989617481685599 2316 90-91 -9.430867186069454 2316 92-93 -8.830278789025142 2316 94-95 -8.209559814049692 2316 96-97 -7.516381074594886 2316 98-99 -6.767254358639388 2316 100-101 -5.816585832372135 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 657021.0 3 40269.0 4 80699.0 5 53533.0 6 47835.0 7 59905.0 8 76457.0 9 89222.0 10 91771.0 11 84157.0 12 75259.0 13 69415.0 14 66108.0 15 68827.0 16 74072.0 17 77450.0 18 79309.0 19 81565.0 20 85666.0 21 89589.0 22 93889.0 23 99015.0 24 104308.0 25 109223.0 26 114625.0 27 120672.0 28 126876.0 29 132036.0 30 134556.0 31 133303.0 32 127311.0 33 121246.0 34 109753.0 35 89191.0 36 58248.0 37 21639.0 38 2364.0 39 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.349197154213925 19.286825955827286 13.50649903405636 23.857477855902427 2 15.681492035594536 20.83843390865889 39.83011543482156 23.649958620925016 3 18.446364702352653 25.906684268970892 30.50466591601189 25.142285112664563 4 12.805743800037838 17.104843097316085 38.25031033966326 31.83910276298282 5 13.330723013662398 37.37970863169874 35.20523638412719 14.084331970511673 6 30.40790191745597 37.109955613380144 18.214106331068205 14.268036138095683 7 26.31161824314534 33.01618331344649 23.081948499162735 17.590249944245436 8 25.410105730752246 34.95880301868739 21.292751017332467 18.3383402332279 9 26.307895224615667 16.083572875087686 21.200305930417628 36.40822596987902 10-11 24.63190692622131 26.09704713718092 28.950204384986993 20.320841551610783 12-13 25.21436288454824 24.22458131150906 28.82769992395351 21.73335587998919 14-15 22.51857016314364 25.363208101642527 26.76031341184707 25.357908323366768 16-17 22.034562341887284 28.26074765917228 27.4525920698363 22.252097929104135 18-19 21.961803664130706 27.090953353367915 29.584736765129353 21.362506217372022 20-21 22.627233468868607 26.422446922352638 29.553946593819674 21.39637301495908 22-23 22.414343615558387 26.33082830444364 29.32640933816968 21.928418741828295 24-25 22.176554679084465 26.548920030907187 29.330408230550997 21.944117059457355 26-27 22.010619596444258 26.784346324736045 29.25672977468046 21.94830430413924 28-29 22.082794439259366 27.05025154544512 29.172655660764914 21.694298354530602 30-31 22.28535634181333 26.81506535251812 29.021662933643373 21.877915372025175 32-33 22.11965920008476 26.989265385865508 28.87466624652163 22.0164091675281 34-35 22.58233047219904 26.935989544442336 28.801215947543202 21.680464035815422 36-37 23.03140660772374 26.93218062354378 28.377079461594967 21.65933330713752 38-39 22.735479461755265 26.96512934239026 28.42282475310557 21.876566442748903 40-41 22.740147978683687 26.875328629749323 28.525209480504053 21.859313911062934 42-43 22.83316185415937 26.954727699628002 28.134001941341534 22.078108504871093 44-45 23.036284406230905 27.148763317616453 27.935143945163215 21.879808330989427 46-47 22.520055964063886 27.160806121971266 28.09610899331455 22.223028920650297 48-49 22.955008942299767 27.346318996468987 27.4872760606298 22.211396000601443 50-51 23.212366947152738 27.439491433945555 27.40409402273633 21.94404759616538 52-53 22.365610950880026 27.500027140773764 27.828083704558804 22.306278203787404 54-55 22.046069334583585 27.44745740267908 28.030855243076292 22.47561801966105 56-57 22.075320151667636 27.706065956812587 27.791733084027275 22.426880807492505 58-59 22.177954169733606 27.4904142144665 27.917521042656418 22.414110573143475 60-61 22.113627713534243 27.5460160658392 27.97948144134722 22.36087477927933 62-63 22.174469989612213 27.473975280507062 27.95767959450798 22.393875135372745 64-65 21.959010563808697 27.369903299886893 27.938782337805378 22.732303798499036 66-67 21.921503228506573 27.492799377190707 27.836438794020275 22.749258600282445 68-69 22.031320933362146 27.38177599438017 27.90229527344616 22.684607798811523 70-71 21.952499172107785 27.326544060711893 27.681923053223983 23.039033713956336 72-73 22.069423423037946 27.477824948089726 27.612500892666937 22.840250736205395 74-75 22.04089892404824 27.62645973071123 27.483628804256945 22.849012540983594 76-77 22.14724661259532 27.39554766776203 27.496248788946442 22.960956930696206 78-79 21.965872465878302 27.53912471196659 27.5021933671244 22.992809455030706 80-81 22.086655911151055 27.57078672630951 27.349012945265844 22.99354441727359 82-83 22.20781027963837 27.496720066340625 27.349536488684638 22.945933165336367 84-85 22.160191689182028 27.551762509273075 27.282501009172456 23.005544792372444 86-87 22.065254869831485 27.568483833714286 27.19160119072376 23.174660105730474 88-89 22.03853059289922 27.410760703662213 27.153153674616306 23.39755502882226 90-91 22.082443304658067 27.34785000285634 27.16981482103136 23.39989187145424 92-93 22.094827309592684 27.175624658131458 27.281286611286788 23.448261420989066 94-95 22.021163196190606 27.081580678710303 27.291318680374154 23.605937444724937 96-97 22.031123665714865 26.92128655609625 27.18083551474909 23.866754263439795 98-99 21.891582003576847 26.98025182958721 27.12462018283362 24.00354598400232 100-101 22.247492607849807 27.076010920405935 26.98818695706164 23.68830951468262 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45652.0 1 49263.0 2 37118.0 3 22486.0 4 28674.5 5 35924.5 6 38113.5 7 36412.5 8 33397.0 9 30504.5 10 27269.0 11 23948.5 12 20668.0 13 17568.5 14 15178.5 15 13378.0 16 12020.5 17 11382.5 18 11228.0 19 11725.0 20 12717.5 21 14014.0 22 15897.5 23 18571.0 24 21758.5 25 25646.5 26 30595.5 27 36420.5 28 42467.5 29 49125.5 30 56194.5 31 63177.5 32 70207.5 33 75647.0 34 79526.5 35 83749.0 36 89108.5 37 93903.5 38 97953.0 39 101295.0 40 103623.5 41 106246.5 42 108920.0 43 110999.0 44 112896.0 45 115098.5 46 116454.5 47 116289.5 48 115320.0 49 112893.0 50 109298.0 51 105470.0 52 101131.5 53 96647.5 54 90868.5 55 83640.0 56 75425.0 57 66959.5 58 58989.5 59 50877.5 60 43110.0 61 35893.5 62 29510.5 63 23728.0 64 18544.0 65 14370.5 66 11006.0 67 8512.0 68 6551.5 69 4994.5 70 3859.5 71 2955.5 72 2249.5 73 1684.5 74 1307.0 75 994.0 76 718.5 77 492.0 78 296.0 79 196.0 80 122.0 81 67.0 82 50.0 83 30.5 84 15.0 85 9.5 86 8.5 87 3.5 88 1.5 89 1.0 90 0.5 91 1.5 92 1.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.709492880759834 2 21.785518192546068 3 17.527507095484008 4 22.827532693411833 5 22.693804207082163 6 16.45914728204192 7 15.261836906232842 8 12.29442581765894 9 3.0831222638711275 10-11 6.622282689207791 12-13 3.632970558112234 14-15 7.5802964821934244 16-17 11.702777361822573 18-19 14.860824947276011 20-21 15.27970740954576 22-23 13.18438755956723 24-25 15.761717191210648 26-27 13.239146838949528 28-29 13.257697996234782 30-31 14.136569082240882 32-33 12.58108790926322 34-35 19.48057026524418 36-37 24.257800227792195 38-39 23.293006587395837 40-41 18.42159279969528 42-43 18.369716290078173 44-45 23.748190594856872 46-47 13.05119291949038 48-49 21.80178715710054 50-51 21.538467492900512 52-53 11.487116821842426 54-55 13.121553855611138 56-57 12.648058921678615 58-59 13.674778727401288 60-61 13.072173077225932 62-63 13.045200495302552 64-65 14.111304808326508 66-67 12.432691997137518 68-69 13.480111691313013 70-71 12.061882389933581 72-73 11.41603519328037 74-75 11.4934028614693 76-77 11.534655830655428 78-79 11.96844594953498 80-81 11.939858482696842 82-83 10.420251777908215 84-85 10.335503685274144 86-87 10.471460979709304 88-89 11.920880248409338 90-91 9.354027349477379 92-93 11.40619907607229 94-95 11.493536323032503 96-97 11.06297597398247 98-99 10.919838447446974 100-101 11.00937781020004 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3746397.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.78864776433726 #Duplication Level Percentage of deduplicated Percentage of total 1 98.80810031602265 95.63502417750816 2 0.8078579341502419 1.5638295406418585 3 0.17109784366791672 0.4968098677203489 4 0.0722267068925051 0.2796290117038682 5 0.04170530736738805 0.20183001523427704 6 0.022811364382883823 0.13247286673673347 7 0.0172775602635312 0.1170590186201822 8 0.011102621047505094 0.08596861422623102 9 0.006455405724024673 0.056232899171865955 >10 0.03798138748583116 0.6338526460446258 >50 0.0017352544132659363 0.11231340549738833 >100 0.0014826298120821694 0.306395004065744 >500 8.28343917312748E-5 0.04634073810548451 >1k 5.5222918968514384E-5 0.15070091184398776 >5k 2.7611459484257192E-5 0.18154128287927093 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6793 0.18132087976794772 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.6692312640651806E-5 0.0 4 0.0 0.0 0.0 2.6692312640651806E-5 0.0 5 0.0 0.0 0.0 2.6692312640651806E-5 0.0 6 0.0 0.0 0.0 2.6692312640651806E-5 0.0 7 0.0 0.0 0.0 2.6692312640651806E-5 0.0 8 0.0 0.0 0.0 2.6692312640651806E-5 0.0 9 0.0 0.0 0.0 5.338462528130361E-5 0.0 10-11 2.6692312640651806E-5 0.0 0.0 5.338462528130361E-5 0.0 12-13 2.6692312640651806E-5 0.0 0.0 5.338462528130361E-5 2.6692312640651806E-5 14-15 2.6692312640651806E-5 0.0 0.0 5.338462528130361E-5 1.0676925056260722E-4 16-17 2.6692312640651806E-5 0.0 0.0 5.338462528130361E-5 1.6015387584391083E-4 18-19 2.6692312640651806E-5 0.0 0.0 5.338462528130361E-5 1.6015387584391083E-4 20-21 2.6692312640651806E-5 0.0 0.0 1.2011540688293312E-4 2.5357697008619213E-4 22-23 2.6692312640651806E-5 0.0 0.0 2.2688465744554034E-4 3.2030775168782167E-4 24-25 2.6692312640651806E-5 0.0 0.0 4.270770022504289E-4 3.2030775168782167E-4 26-27 2.6692312640651806E-5 0.0 0.0 6.272693470553174E-4 3.4700006432847346E-4 28-29 2.6692312640651806E-5 0.0 0.0 0.0013479617883529163 3.4700006432847346E-4 30-31 2.6692312640651806E-5 0.0 0.0 0.0050048086201222135 3.8703853328945115E-4 32-33 2.6692312640651806E-5 0.0 0.0 0.014053502605303175 4.13730845930103E-4 34-35 2.6692312640651806E-5 0.0 0.0 0.026331966420003006 4.270770022504289E-4 36-37 2.6692312640651806E-5 1.3346156320325903E-5 0.0 0.04404231585707548 4.270770022504289E-4 38-39 2.6692312640651806E-5 2.6692312640651806E-5 0.0 0.06952012827257763 4.537693148910807E-4 40-41 2.6692312640651806E-5 2.6692312640651806E-5 0.0 0.09710663338669126 4.804616275317325E-4 42-43 4.003846896097771E-5 2.6692312640651806E-5 0.0 0.12828325455097256 5.071539401723843E-4 44-45 5.338462528130361E-5 2.6692312640651806E-5 0.0 0.16700045403623803 5.071539401723843E-4 46-47 5.338462528130361E-5 2.6692312640651806E-5 0.0 0.21157661614612655 5.071539401723843E-4 48-49 5.338462528130361E-5 2.6692312640651806E-5 0.0 0.2588620479890412 6.139231907349915E-4 50-51 5.338462528130361E-5 2.6692312640651806E-5 0.0 0.3139416351230262 6.139231907349915E-4 52-53 5.338462528130361E-5 2.6692312640651806E-5 0.0 0.3877191872617878 7.073462849772729E-4 54-55 5.338462528130361E-5 2.6692312640651806E-5 0.0 0.4736150493394053 7.206924412975987E-4 56-57 5.338462528130361E-5 2.6692312640651806E-5 0.0 0.5695205286572673 7.340385976179246E-4 58-59 5.338462528130361E-5 5.338462528130361E-5 0.0 0.6723660092616986 7.607309102585765E-4 60-61 5.338462528130361E-5 5.338462528130361E-5 0.0 0.7820180295894963 7.874232228992282E-4 62-63 5.338462528130361E-5 5.338462528130361E-5 0.0 0.8964212815673299 8.27461691860206E-4 64-65 5.338462528130361E-5 5.338462528130361E-5 0.0 1.0226759203576128 8.541540045008578E-4 66-67 5.338462528130361E-5 5.338462528130361E-5 0.0 1.164291985072591 8.675001608211836E-4 68-69 5.338462528130361E-5 5.338462528130361E-5 0.0 1.3139157435797648 8.808463171415096E-4 70-71 5.338462528130361E-5 5.338462528130361E-5 0.0 1.468143926017451 8.808463171415096E-4 72-73 8.007693792195542E-5 5.338462528130361E-5 2.6692312640651806E-5 1.636131995621393 9.208847861024872E-4 74-75 9.342309424228133E-5 6.673078160162951E-5 2.6692312640651806E-5 1.809885604755716 9.876155677041166E-4 76-77 1.0676925056260722E-4 8.007693792195542E-5 2.6692312640651806E-5 2.0078624876114306 0.0010143078803447685 78-79 1.0676925056260722E-4 8.007693792195542E-5 2.6692312640651806E-5 2.2217079503320125 0.0010810386619463982 80-81 1.0676925056260722E-4 9.342309424228133E-5 2.6692312640651806E-5 2.444962453258424 0.001121077130907376 82-83 1.3346156320325902E-4 1.3346156320325902E-4 2.6692312640651806E-5 2.683565035953211 0.0012011540688293312 84-85 1.3346156320325902E-4 1.3346156320325902E-4 5.338462528130361E-5 2.924543234473015 0.001227846381469983 86-87 1.3346156320325902E-4 1.3346156320325902E-4 5.338462528130361E-5 3.18640816763413 0.001227846381469983 88-89 1.3346156320325902E-4 1.3346156320325902E-4 5.338462528130361E-5 3.457628756375793 0.001227846381469983 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3320 0.0 55.17533 1 GTATCAA 6485 0.0 46.68933 1 TCAACGC 8325 0.0 36.76386 4 ATCAACG 8215 0.0 36.532753 3 TATCAAC 8445 0.0 35.188923 2 CAACGCA 8680 0.0 33.89183 5 AACGCAG 9385 0.0 32.111053 6 GTACATG 7145 0.0 27.686003 1 ACGCAGA 10925 0.0 27.345413 7 TACATGG 7300 0.0 26.539864 2 GTGGTAT 1645 0.0 26.382076 1 CGCAGAG 11335 0.0 26.232815 8 ACATGGG 7720 0.0 24.827862 3 GCAGAGT 12120 0.0 22.613014 9 CATGGGG 5970 0.0 20.438906 4 TGGTATC 1825 0.0 19.904898 2 AGAGTAC 10920 0.0 19.804907 10-11 CAGAGTA 11620 0.0 19.068106 10-11 GAGTACT 8425 0.0 18.590668 12-13 AGTACTT 8830 0.0 17.971716 12-13 >>END_MODULE