FastQCFastQC Report
Sat 18 Jun 2016
SRR1930924_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930924_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3746397
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90010.2402575060785069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78430.2093478080406321No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49200.1313261781920069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47750.060.253511
GTATCAA94650.044.6429061
TCAACGC124000.033.840624
ATCAACG124700.033.5754173
CAACGCA125600.033.363275
TATCAAC126600.033.0724072
AACGCAG131800.031.865426
ACGCAGA152500.027.3533677
CGCAGAG155700.026.6997578
GTACATG104150.026.3026581
TACATGG106000.024.9448132
ACATGGG107000.023.9124643
GCAGAGT171650.023.389369
GTGGTAT22050.023.0387171
TATCACG3401.8189894E-1220.943212
AGAGTAC160350.020.7463110-11
CATGGGG76150.020.6954984
CAGAGTA165500.020.12940210-11
ATAACGC3102.6193447E-1019.9067763
GAGTACT121500.019.66595312-13
Produced by FastQC (version 0.11.5)