##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930924_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3746397 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.261781119299425 31.0 31.0 34.0 30.0 34.0 2 31.384628751304252 31.0 31.0 34.0 30.0 34.0 3 31.46556197861572 31.0 31.0 34.0 30.0 34.0 4 35.33784273263085 37.0 35.0 37.0 33.0 37.0 5 35.126340054190734 37.0 35.0 37.0 32.0 37.0 6 35.16373625112341 37.0 35.0 37.0 32.0 37.0 7 35.03916055879823 37.0 35.0 37.0 32.0 37.0 8 35.01982438059821 37.0 35.0 37.0 32.0 37.0 9 36.52674823303563 38.0 35.0 39.0 32.0 39.0 10-11 36.45776528755495 38.0 35.0 39.0 32.0 39.0 12-13 36.35302598736867 38.0 35.0 39.0 32.0 39.0 14-15 37.370085044377305 39.0 36.0 41.0 32.0 41.0 16-17 37.34067091661669 39.0 36.0 41.0 32.0 41.0 18-19 37.34695949201326 39.0 36.0 41.0 32.0 41.0 20-21 37.31604458897442 39.0 36.0 40.5 31.5 41.0 22-23 37.190439374150685 39.0 36.0 40.0 31.0 41.0 24-25 37.065629323320515 39.0 36.0 40.0 31.0 41.0 26-27 36.79310908587638 39.0 35.5 40.0 30.5 41.0 28-29 36.601183483757865 38.5 35.0 40.0 30.0 41.0 30-31 36.34309431168133 38.0 35.0 40.0 30.0 41.0 32-33 36.077712132483555 38.0 35.0 40.0 30.0 41.0 34-35 36.217427037230706 38.0 35.0 40.0 30.0 41.0 36-37 36.23753555749698 38.0 35.0 40.0 30.0 41.0 38-39 36.18568440557688 38.0 35.0 40.0 30.0 41.0 40-41 36.06947915557268 38.0 35.0 40.0 29.5 41.0 42-43 35.90101235934152 38.0 35.0 40.0 29.0 41.0 44-45 35.68721414201431 38.0 34.5 40.0 28.0 41.0 46-47 35.42587758318192 38.0 34.0 40.0 27.0 41.0 48-49 35.19274865957879 38.0 34.0 40.0 27.0 41.0 50-51 34.906056672584356 38.0 33.5 40.0 26.0 41.0 52-53 34.684333507634136 38.0 33.0 40.0 26.0 41.0 54-55 34.50329930330395 38.0 33.0 40.0 25.5 41.0 56-57 34.24398789023161 37.0 33.0 40.0 25.0 41.0 58-59 33.9971085552332 37.0 33.0 40.0 24.0 41.0 60-61 33.684839460420235 36.5 32.0 40.0 23.5 41.0 62-63 33.371033422245425 36.0 32.0 39.0 23.0 41.0 64-65 33.055224259468496 36.0 31.5 39.0 22.5 40.5 66-67 32.71384266002775 35.0 31.0 39.0 22.0 40.0 68-69 32.34385637720722 35.0 31.0 38.5 21.5 40.0 70-71 31.93815844930476 35.0 31.0 38.0 20.0 40.0 72-73 31.52512640811959 35.0 30.0 37.0 19.5 39.5 74-75 31.01545164594142 34.0 30.0 36.5 18.0 39.0 76-77 29.636033100603058 32.5 28.5 35.0 16.0 38.0 78-79 30.39117090367091 34.0 29.5 36.0 18.0 38.0 80-81 30.393540914110275 34.0 30.0 35.5 18.0 37.0 82-83 30.125700506379864 34.0 30.0 35.0 16.0 37.0 84-85 29.81404560168076 34.0 29.0 35.0 12.0 36.5 86-87 29.48519617648637 34.0 29.0 35.0 7.5 36.0 88-89 29.14489921916978 34.0 29.0 35.0 6.0 36.0 90-91 28.816115990910735 34.0 29.0 35.0 2.0 35.0 92-93 28.47533056427282 33.5 29.0 35.0 2.0 35.0 94-95 28.06926735207187 33.0 28.0 35.0 2.0 35.0 96-97 27.618499721198795 33.0 27.0 35.0 2.0 35.0 98-99 27.13865174993467 33.0 27.0 35.0 2.0 35.0 100-101 25.971654365514386 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49019254355516395 1101 2 0.6266934937863091 1101 3 0.5723710619187834 1101 4 0.4492718112085683 1101 5 0.5623668260104466 1101 6 0.5484454401866188 1101 7 0.5450796814919414 1101 8 0.5322380604754855 1101 9 0.7876516511737393 1101 10-11 0.7268737116779178 1101 12-13 0.7358618044883869 1101 14-15 0.7608072174081641 1101 16-17 0.7551993841953646 1101 18-19 0.8017421092787202 1101 20-21 0.8303772611610469 1101 22-23 0.9726698784758483 1101 24-25 0.7375163666834297 1101 26-27 0.7693550229156827 1101 28-29 0.8091636149667991 1101 30-31 0.801723487517215 1101 32-33 0.8324146554796386 1101 34-35 0.6775647072411886 1101 36-37 0.7990968083912477 1101 38-39 0.8316081055255253 1101 40-41 0.9121196807882939 1101 42-43 0.8302376783345693 1101 44-45 0.7894074849849346 1101 46-47 0.7988769083546714 1101 48-49 0.7812932235409491 1101 50-51 0.7107893981467086 1101 52-53 0.6850936684159095 1101 54-55 0.8113690457847511 1101 56-57 0.823703102863 1101 58-59 0.659275678426603 1101 60-61 0.649605366827636 1101 62-63 0.7550563236142978 1101 64-65 0.7662639192462493 1101 66-67 0.7866352188934869 1101 68-69 0.7599984078210369 1101 70-71 0.7557539809050269 1101 72-73 0.7563301475066417 1101 74-75 0.840402308889356 1101 76-77 0.8706093562298243 1101 78-79 0.9634073049168776 1101 80-81 0.8311674917995653 1101 82-83 0.895135563022631 1101 84-85 0.851940435499646 1101 86-87 0.7587096360890513 1101 88-89 0.7787028500797071 1101 90-91 0.8127045417357692 1101 92-93 0.9608230548707226 1101 94-95 0.9213162501060559 1101 96-97 1.0946988981779917 1101 98-99 1.1084029274261873 1101 100-101 1.4481619021293461 1102 1 0.5236224895753487 1102 2 0.5950266046068364 1102 3 0.547758070764143 1102 4 0.43990622547956093 1102 5 0.5312690514629423 1102 6 0.5118603400798278 1102 7 0.5895895303348055 1102 8 0.5470890105518933 1102 9 0.7091234201098544 1102 10-11 0.6653568871675972 1102 12-13 0.7582779477899209 1102 14-15 0.7938998349853605 1102 16-17 0.7915490662393267 1102 18-19 0.7857814401480923 1102 20-21 0.7752556943532412 1102 22-23 0.8846315131038196 1102 24-25 0.8488110004154876 1102 26-27 0.8622263591358248 1102 28-29 0.9054183685448791 1102 30-31 0.9852979675890481 1102 32-33 1.0232121699414023 1102 34-35 0.9208290340029137 1102 36-37 1.0189818189069726 1102 38-39 1.0150125412638076 1102 40-41 0.9569649694997509 1102 42-43 0.9022224349242052 1102 44-45 1.003570914078196 1102 46-47 1.0178571464574233 1102 48-49 0.8421946996652281 1102 50-51 0.9667417488334777 1102 52-53 0.9784069964195865 1102 54-55 0.9518431709353408 1102 56-57 0.9974341152449284 1102 58-59 1.0914928182852677 1102 60-61 1.1145023254819435 1102 62-63 0.9791699611698519 1102 64-65 0.8928293476715297 1102 66-67 1.054169215775012 1102 68-69 1.0667279867749997 1102 70-71 0.9561737093018792 1102 72-73 0.8504006921526077 1102 74-75 1.0221185407069235 1102 76-77 1.1434075000226862 1102 78-79 1.2277742351655796 1102 80-81 1.0962499375150365 1102 82-83 1.1031562586020165 1102 84-85 1.1999688146713403 1102 86-87 1.212707813469482 1102 88-89 1.1450534880328753 1102 90-91 1.1060913425473942 1102 92-93 1.1914583393386948 1102 94-95 1.2571128050267397 1102 96-97 1.2995199492161333 1102 98-99 1.479338878749779 1102 100-101 1.8115407593064887 1103 1 0.3594893547046958 1103 2 0.4473731973246018 1103 3 0.4271492430376824 1103 4 0.2536712351618604 1103 5 0.2828503522084489 1103 6 0.3543341328662777 1103 7 0.3670940941402847 1103 8 0.4326684451452394 1103 9 0.5293070463734892 1103 10-11 0.50970216700739 1103 12-13 0.5480490526742514 1103 14-15 0.5906782670430459 1103 16-17 0.6107876040833204 1103 18-19 0.5954859966755066 1103 20-21 0.6221765962820314 1103 22-23 0.5729383845502412 1103 24-25 0.5252609515358699 1103 26-27 0.5473514093491687 1103 28-29 0.6177355027682836 1103 30-31 0.650847003899166 1103 32-33 0.7116325579894038 1103 34-35 0.610604767170706 1103 36-37 0.7996667200683873 1103 38-39 0.6980924554674388 1103 40-41 0.7380446526425146 1103 42-43 0.7223860641767956 1103 44-45 0.8023995697243578 1103 46-47 0.7496012002613952 1103 48-49 0.7226647212839055 1103 50-51 0.746130200665597 1103 52-53 0.6820574401871724 1103 54-55 0.6922120486613963 1103 56-57 0.7954371699120202 1103 58-59 0.738362879756643 1103 60-61 0.6010490713217038 1103 62-63 0.4375864946703203 1103 64-65 0.44509771886551874 1103 66-67 0.3840089252460004 1103 68-69 0.5340825129359317 1103 70-71 0.4248125664621014 1103 72-73 0.4144805840980901 1103 74-75 0.6565834952384542 1103 76-77 0.7777390822632206 1103 78-79 0.7613734180438954 1103 80-81 0.6712646261322526 1103 82-83 0.6385725197050398 1103 84-85 0.618501168316012 1103 86-87 0.6795532991089104 1103 88-89 0.6350673481520168 1103 90-91 0.6068909403460268 1103 92-93 0.7359189729613007 1103 94-95 0.718852516723544 1103 96-97 0.701355222601066 1103 98-99 0.8932820243502775 1103 100-101 0.9319755494420967 1104 1 0.3810302134116341 1104 2 0.46196750504785555 1104 3 0.42542425921046956 1104 4 0.20243632749623686 1104 5 0.2884625804648877 1104 6 0.31917008102638533 1104 7 0.3808947801925484 1104 8 0.39907396889884694 1104 9 0.522304774029223 1104 10-11 0.5073550405987319 1104 12-13 0.5399403715580817 1104 14-15 0.5674234328752448 1104 16-17 0.6109871477117892 1104 18-19 0.652344276869691 1104 20-21 0.5704546703343638 1104 22-23 0.5620558329468324 1104 24-25 0.6250489045777172 1104 26-27 0.7481185633738789 1104 28-29 0.549361452069995 1104 30-31 0.5969562598966931 1104 32-33 0.7370390181336788 1104 34-35 0.6598469226903845 1104 36-37 0.7167445016515259 1104 38-39 0.7385748053867118 1104 40-41 0.782940677942733 1104 42-43 0.7150276168185243 1104 44-45 0.7930983495624702 1104 46-47 0.7476893799940427 1104 48-49 0.8005016180662068 1104 50-51 0.726872184944007 1104 52-53 0.771646073845055 1104 54-55 0.8377274644514685 1104 56-57 0.8502017879353048 1104 58-59 0.8628996789586054 1104 60-61 0.8436457071105821 1104 62-63 0.7515206741528928 1104 64-65 0.7926970116119563 1104 66-67 0.657036185222843 1104 68-69 0.6427959067201456 1104 70-71 0.8107368751095123 1104 72-73 0.6749769594915414 1104 74-75 0.9395513779898401 1104 76-77 1.0175929020304402 1104 78-79 1.0484390632428884 1104 80-81 0.8271899472775566 1104 82-83 0.9489724359870912 1104 84-85 1.0113706688895334 1104 86-87 0.8993440314234888 1104 88-89 1.037879612015768 1104 90-91 1.1137567633097518 1104 92-93 0.9873086772847337 1104 94-95 0.9448115017249847 1104 96-97 0.927231916666333 1104 98-99 1.1680826506040063 1104 100-101 1.4527836382809731 1105 1 0.17190321803102293 1105 2 0.27098484508654863 1105 3 0.27517950407589 1105 4 0.10486394866853743 1105 5 0.19084805680390815 1105 6 0.24296001227122588 1105 7 0.2791217037300058 1105 8 0.2516596135021061 1105 9 0.3591317958783691 1105 10-11 0.30918077840902214 1105 12-13 0.2950828289721059 1105 14-15 0.42537407536731564 1105 16-17 0.3520246747317515 1105 18-19 0.375650842325733 1105 20-21 0.36697626424408014 1105 22-23 0.3639256849751362 1105 24-25 0.4307488160341748 1105 26-27 0.37845579293796305 1105 28-29 0.43568305102133564 1105 30-31 0.4650289490994197 1105 32-33 0.5810757605507604 1105 34-35 0.521883841564339 1105 36-37 0.6234563223713465 1105 38-39 0.4618764473809307 1105 40-41 0.5224747766577522 1105 42-43 0.47043692586382946 1105 44-45 0.6733469568135888 1105 46-47 0.6663555629798168 1105 48-49 0.6510182270692866 1105 50-51 0.6766168345195709 1105 52-53 0.6606690778342426 1105 54-55 0.6659803759898537 1105 56-57 0.7923607613974468 1105 58-59 0.7840803723710934 1105 60-61 0.7596863063707247 1105 62-63 0.635170014960444 1105 64-65 0.6088923651243903 1105 66-67 0.6159567890834694 1105 68-69 0.666605374261529 1105 70-71 0.6669115264496384 1105 72-73 0.7410508019659723 1105 74-75 0.9119776284703676 1105 76-77 0.8440570214161163 1105 78-79 0.8996146283775346 1105 80-81 0.8268970997678053 1105 82-83 0.8928173997189006 1105 84-85 0.9200231213249523 1105 86-87 0.9590392336871787 1105 88-89 0.9700431702055674 1105 90-91 0.8630532990832336 1105 92-93 0.7440569710098011 1105 94-95 0.5715483021207532 1105 96-97 0.5232222887382534 1105 98-99 0.7416604397254396 1105 100-101 1.10006072756185 1106 1 0.05745904901099763 1106 2 0.10920510317781762 1106 3 0.12194649450987427 1106 4 0.027904705230696436 1106 5 0.0972715963659212 1106 6 0.1406468526783229 1106 7 0.11513370851731963 1106 8 0.10794750231309536 1106 9 0.1613014475205503 1106 10-11 0.08901222119872187 1106 12-13 0.09659165210045018 1106 14-15 0.2333294773948822 1106 16-17 0.13195623345316676 1106 18-19 0.19085070920956326 1106 20-21 0.11222912160345544 1106 22-23 0.21826777629809868 1106 24-25 0.16739841990457904 1106 26-27 0.2957234720174924 1106 28-29 0.12934426783291286 1106 30-31 0.1621269937719063 1106 32-33 0.16240341693058014 1106 34-35 0.11196948757569913 1106 36-37 0.22989882059587075 1106 38-39 0.20150443685532338 1106 40-41 0.13956302010725352 1106 42-43 0.2812069795918717 1106 44-45 0.15451812819026145 1106 46-47 0.026709362544174553 1106 48-49 0.1769942560202793 1106 50-51 0.16860451848576474 1106 52-53 -0.022901276799203174 1106 54-55 0.2251263972306532 1106 56-57 0.40703760491193464 1106 58-59 0.27303733164495014 1106 60-61 0.013346050590996583 1106 62-63 0.037513196659958226 1106 64-65 -0.05743973690970705 1106 66-67 0.06925175647102577 1106 68-69 0.04183570426898342 1106 70-71 -0.046273057420393116 1106 72-73 -0.04251870358298859 1106 74-75 0.16245146830414114 1106 76-77 0.2671964998906837 1106 78-79 0.2841376811480103 1106 80-81 0.16904171228873466 1106 82-83 0.20536799628312608 1106 84-85 0.2490323968759327 1106 86-87 0.2656466824138839 1106 88-89 0.26864223149831545 1106 90-91 0.17821502052978744 1106 92-93 0.31440710557068385 1106 94-95 0.32901929511365324 1106 96-97 0.370774112039463 1106 98-99 0.40433628258753274 1106 100-101 0.5603178075324244 1107 1 0.10198171427687086 1107 2 0.19120219830145402 1107 3 0.20940277503849813 1107 4 0.056214707576828005 1107 5 0.21063481461087008 1107 6 0.19362395747175754 1107 7 0.18457616506098873 1107 8 0.1400726756293551 1107 9 0.1648113015241961 1107 10-11 0.2331515249235636 1107 12-13 0.1518127689678721 1107 14-15 0.19539596542099957 1107 16-17 0.178823986325213 1107 18-19 0.1994159998226266 1107 20-21 0.2961500702083981 1107 22-23 0.32137490209852615 1107 24-25 0.2797446117179092 1107 26-27 0.21253320605850234 1107 28-29 0.3661123494094696 1107 30-31 0.25484233752954566 1107 32-33 0.3744039968237729 1107 34-35 0.2527897757713049 1107 36-37 0.2249130424888861 1107 38-39 0.3565736084245188 1107 40-41 0.26287455457154607 1107 42-43 0.39171616664456366 1107 44-45 0.34846492114478167 1107 46-47 0.2737345817501904 1107 48-49 0.35328031305638774 1107 50-51 0.32877849628258815 1107 52-53 0.20056153896346274 1107 54-55 0.3743729664418183 1107 56-57 0.33754559430565223 1107 58-59 0.3739025972227594 1107 60-61 0.29194569212945254 1107 62-63 0.3119938258170265 1107 64-65 0.3530652571069126 1107 66-67 0.412671308954792 1107 68-69 0.5088007282000362 1107 70-71 0.329300872076594 1107 72-73 0.2903525350423273 1107 74-75 0.6482760552839402 1107 76-77 0.5513799497682861 1107 78-79 0.6341323076677696 1107 80-81 0.6295086984622884 1107 82-83 0.5677002318033821 1107 84-85 0.6094394017300182 1107 86-87 0.5548489874278708 1107 88-89 0.5361669089296512 1107 90-91 0.5145044442910454 1107 92-93 0.5082841785413166 1107 94-95 0.5216500157797377 1107 96-97 0.6175368668616983 1107 98-99 0.7027257632560371 1107 100-101 1.0148584721126603 1108 1 0.2678168085073658 1108 2 0.3319267880685004 1108 3 0.3277333385181933 1108 4 0.18014069590424242 1108 5 0.2016118961984077 1108 6 0.1399197374080572 1108 7 0.215828422423904 1108 8 0.3277779030470356 1108 9 0.2730055813018524 1108 10-11 0.34037247295558615 1108 12-13 0.3512001961744673 1108 14-15 0.4227708714592353 1108 16-17 0.5205197013234226 1108 18-19 0.48761859285925624 1108 20-21 0.5057899004818225 1108 22-23 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-0.4667524693937253 2316 72-73 -0.43104707049082336 2316 74-75 -0.25607435687200564 2316 76-77 -0.5323538204857279 2316 78-79 -0.406782130172747 2316 80-81 -0.5542106994008691 2316 82-83 -0.6839824434955695 2316 84-85 -0.7079047573303363 2316 86-87 -0.507736362462456 2316 88-89 -0.6632263550624238 2316 90-91 -0.3800501884833132 2316 92-93 -0.593566543173818 2316 94-95 -0.4884814142068201 2316 96-97 -0.2218421574077425 2316 98-99 -0.19944380442244736 2316 100-101 -0.5107451683033908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 23.0 4 82.0 5 137.0 6 287.0 7 780.0 8 1906.0 9 3332.0 10 5790.0 11 9051.0 12 12800.0 13 16050.0 14 18057.0 15 22173.0 16 25917.0 17 29445.0 18 25817.0 19 21736.0 20 21782.0 21 22949.0 22 25428.0 23 29398.0 24 36124.0 25 44540.0 26 56468.0 27 72403.0 28 91149.0 29 112301.0 30 137014.0 31 166142.0 32 199264.0 33 240078.0 34 293930.0 35 366676.0 36 469493.0 37 593986.0 38 490070.0 39 83800.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.30195024758205 20.498223661886247 14.254382510591684 23.945443579940022 2 15.706501617736834 21.719154692798167 39.11530442307749 23.45903926638751 3 18.203432367654607 26.227371868124006 31.102211609018767 24.466984155202617 4 13.102914604047971 17.892453398991787 37.88523317660146 31.119398820358786 5 12.961928371023076 38.04202669650562 35.35267687095621 13.643368061515087 6 30.440428614593866 38.045070515770355 17.725408632723287 13.78909223691249 7 26.098435376709944 33.236333469197206 23.065281122102117 17.599950031990737 8 25.233364216339062 35.6825237688371 20.990647814420093 18.093464200403748 9 25.769345854163344 15.836202089634387 21.05270744130961 37.34174461489266 10-11 24.291726157158465 26.457260135538224 29.075682582491925 20.175331124811386 12-13 24.915552195883137 24.25709288150722 29.04553361536431 21.781821307245337 14-15 22.159533834712903 25.75775148199076 26.70841166972049 25.374303013575844 16-17 21.732560644267014 28.589869146275742 27.56304257130251 22.114527638154737 18-19 21.66209293889569 27.427926084715526 29.623902645661953 21.28607833072683 20-21 22.331496101454277 26.644092977866467 29.57931313739574 21.44509778328351 22-23 22.127199046763877 26.579945099236706 29.422543692658227 21.870312161341193 24-25 21.934080019474823 26.797460638956238 29.466894068680517 21.801565272888425 26-27 21.88210155706237 26.981095435717872 29.336701974672465 21.800101032547296 28-29 21.943647678274093 27.136627914736145 29.23855579525271 21.681168611737046 30-31 22.226942636027665 26.815571919953353 29.223129778501367 21.73435566551762 32-33 21.958797910861186 27.00382118840628 29.032021225116388 22.005359675616145 34-35 22.248845500257417 27.030637473933073 29.05177452653731 21.668742499272202 36-37 22.271217811165958 26.890183535044198 29.032831523002674 21.80576713078717 38-39 22.07826960033195 26.953079800014496 29.060372149425117 21.90827845022844 40-41 22.223642882062663 26.88781446668889 29.029726105977012 21.85881654527143 42-43 22.186132480954004 27.017938566436285 28.699931100998565 22.095997851611145 44-45 22.074721328621205 27.145236392505335 28.779411752928862 22.000630525944594 46-47 22.28183999488533 27.205898742594435 28.387999049141015 22.124262213379218 48-49 22.232790485573144 27.346515959019403 28.181309398930836 22.239384156476618 50-51 22.264472572417855 27.44179634279293 28.114543690200588 22.17918739458863 52-53 22.42061372539175 27.406758649274295 28.049371928141614 22.12325569719234 54-55 22.112116502874102 27.39083261762243 28.146140113458312 22.35091076604516 56-57 22.144421679269083 27.599410033435888 27.942391500210224 22.313776787084805 58-59 22.066762956397305 27.501935583772063 28.14414893048985 22.28715252934078 60-61 22.131944596526896 27.5794978733087 28.132603195138017 22.15595433502639 62-63 22.16208474872673 27.534739288176745 28.080077945219518 22.223098017877007 64-65 21.90348352467141 27.50978333900474 28.033115655627 22.553617480696854 66-67 21.86400692734346 27.605185780882014 27.945901306198657 22.584905985575872 68-69 21.997407505088393 27.54957391643359 28.005251258503698 22.447767319974314 70-71 21.98386614559691 27.394823091615848 27.889437703673682 22.73187305911356 72-73 21.987813663537768 27.54242392755191 27.789278577535974 22.680483831374353 74-75 21.944528246605344 27.75543623317054 27.651083032370227 22.64895248785389 76-77 21.960822159362944 27.617969674511166 27.615761137484913 22.805447028640977 78-79 21.82879622563038 27.740103131008738 27.66202211414564 22.769078529215246 80-81 21.9195465256446 27.797005309681598 27.550779902860477 22.73266826181332 82-83 21.97658176642785 27.693274364676245 27.55293953096802 22.77720433792788 84-85 21.959120439900165 27.720791891833095 27.411681441534935 22.9084062267318 86-87 21.870146169773253 27.797894884071283 27.390476770080696 22.941482176074775 88-89 21.7937383954892 27.74938153763038 27.327455138122374 23.12942492875805 90-91 21.771030952520142 27.66392332195367 27.40472436152569 23.160321364000495 92-93 21.819071322402138 27.5376247846699 27.485698610607756 23.15760528232021 94-95 21.672227224158487 27.512120538329942 27.486055008011846 23.329597229499722 96-97 21.64689166684684 27.302592330711345 27.504799945120606 23.545716057321208 98-99 21.490234483958854 27.42182689127714 27.400072656475004 23.687865968289 100-101 21.49373117691478 27.41817004444537 27.378905652550973 23.709193126088877 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 383.0 1 317.5 2 401.5 3 585.0 4 529.0 5 514.0 6 767.0 7 1457.0 8 2359.5 9 2886.0 10 2724.0 11 2417.5 12 2509.0 13 2762.0 14 3142.0 15 3598.0 16 4077.5 17 4496.0 18 4912.0 19 5578.0 20 6420.0 21 7431.5 22 8920.0 23 11022.0 24 14034.0 25 18300.5 26 23795.0 27 30185.0 28 37074.5 29 45340.5 30 54489.0 31 63801.5 32 74302.5 33 80146.5 34 82433.5 35 86213.5 36 93134.0 37 101472.5 38 107440.5 39 111293.0 40 113779.5 41 117332.0 42 121070.0 43 125229.5 44 128776.5 45 133115.5 46 137168.0 47 138718.5 48 139649.5 49 138618.0 50 134598.0 51 129861.0 52 126782.0 53 124473.0 54 120512.5 55 111651.5 56 100212.0 57 88647.5 58 78430.5 59 68265.0 60 56811.5 61 47201.0 62 39090.5 63 30314.5 64 22494.0 65 16885.0 66 12492.5 67 9897.0 68 7804.0 69 5892.0 70 4514.0 71 3314.5 72 2479.5 73 1945.0 74 1504.0 75 1194.5 76 899.5 77 546.5 78 312.5 79 184.0 80 111.0 81 65.0 82 33.0 83 20.0 84 12.0 85 5.5 86 3.5 87 3.5 88 1.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02055308073330189 2 0.003042923641034306 3 0.003203077516878217 4 0.036301545191286456 5 0.0017083080090017154 6 2.6692312640651802E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.3346156320325901E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.6692312640651802E-5 24-25 5.738847217740138E-4 26-27 0.0021220388549318186 28-29 8.007693792195542E-5 30-31 0.006846578192327188 32-33 0.022274734898623933 34-35 0.013626425603052746 36-37 7.473847539382506E-4 38-39 6.806539723366211E-4 40-41 0.0025090773882212696 42-43 0.008915232421977703 44-45 0.008488155419727274 46-47 0.008287963074922385 48-49 0.010156424959768012 50-51 0.1915840739782783 52-53 0.05127593258269212 54-55 0.018791388099018872 56-57 0.011264155934355062 58-59 0.020206080668973416 60-61 0.05560008723047771 62-63 0.10058998018629632 64-65 0.10510098102256649 66-67 0.110119135799009 68-69 0.10826402007048372 70-71 0.09566524850409608 72-73 0.07196247487919727 74-75 0.24261977574720459 76-77 0.2907460154382998 78-79 0.0076873860405077205 80-81 8.808463171415095E-4 82-83 0.0 84-85 4.003846896097771E-5 86-87 0.0 88-89 2.4023081376586627E-4 90-91 0.006406155033756434 92-93 0.01853781112893268 94-95 0.0018684618848456263 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3746397.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.8433358069527 #Duplication Level Percentage of deduplicated Percentage of total 1 78.03413040857458 44.35720279218145 2 12.115570185639587 13.773788491100309 3 3.8096073165325333 6.4965236395884824 4 1.7973106788592568 4.086605378712755 5 0.987011255988271 2.8052506134691724 6 0.6726787096780442 2.2942381070650026 7 0.4621473121774485 1.8388996400868316 8 0.3311302352002739 1.505803772425952 9 0.2574039928450751 1.316853144300875 >10 1.434219757458049 14.44813329054517 >50 0.07154149252588521 2.7642608993856497 >100 0.024523285850518453 2.434554321582626 >500 0.0018795646288225457 0.7170347968023597 >1k 7.518258147358369E-4 0.710944848672531 >5k 9.397822684197957E-5 0.44990626408085466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9001 0.2402575060785069 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7843 0.2093478080406321 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4920 0.1313261781920069 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 2.6692312640651806E-5 0.0 4 0.0 0.0 0.0 8.007693792195542E-5 0.0 5 0.0 0.0 0.0 8.007693792195542E-5 0.0 6 2.6692312640651806E-5 0.0 0.0 8.007693792195542E-5 0.0 7 2.6692312640651806E-5 0.0 0.0 8.007693792195542E-5 0.0 8 2.6692312640651806E-5 0.0 0.0 8.007693792195542E-5 0.0 9 2.6692312640651806E-5 0.0 0.0 8.007693792195542E-5 0.0 10-11 2.6692312640651806E-5 0.0 0.0 8.007693792195542E-5 0.0 12-13 2.6692312640651806E-5 1.3346156320325903E-5 0.0 8.007693792195542E-5 2.6692312640651803E-4 14-15 2.6692312640651806E-5 2.6692312640651806E-5 0.0 9.342309424228133E-5 3.8703853328945115E-4 16-17 2.6692312640651806E-5 2.6692312640651806E-5 0.0 1.0676925056260722E-4 4.270770022504289E-4 18-19 2.6692312640651806E-5 2.6692312640651806E-5 0.0 1.0676925056260722E-4 4.537693148910807E-4 20-21 2.6692312640651806E-5 2.6692312640651806E-5 0.0 1.8684618848456263E-4 5.205000964927102E-4 22-23 2.6692312640651806E-5 2.6692312640651806E-5 0.0 4.4042315857075473E-4 5.872308780943396E-4 24-25 2.6692312640651806E-5 2.6692312640651806E-5 0.0 6.272693470553174E-4 6.139231907349915E-4 26-27 2.6692312640651806E-5 2.6692312640651806E-5 0.0 0.0010810386619463982 7.073462849772729E-4 28-29 2.6692312640651806E-5 4.003846896097771E-5 0.0 0.002882769765190395 7.473847539382505E-4 30-31 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.01162450215500386 7.473847539382505E-4 32-33 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.03169712126077402 7.740770665789023E-4 34-35 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.06013778037938852 8.541540045008578E-4 36-37 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.10205805738153217 8.541540045008578E-4 38-39 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.1630900302343825 8.808463171415096E-4 40-41 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.23024788883826247 8.941924734618355E-4 42-43 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.303491594724211 9.075386297821614E-4 44-45 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.3902949954316107 9.60923255063465E-4 46-47 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.4898172831122809 0.0010543463493057463 48-49 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.5930898407189629 0.0011344232872277015 50-51 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.6999124759068514 0.0012011540688293312 52-53 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.8110192272735643 0.0012945771630716125 54-55 2.6692312640651806E-5 5.338462528130361E-5 0.0 0.9404102127991241 0.001374654100993568 56-57 2.6692312640651806E-5 5.338462528130361E-5 0.0 1.0837212393667834 0.0014146925699545457 58-59 2.6692312640651806E-5 5.338462528130361E-5 0.0 1.2395909990318699 0.0014413848825951974 60-61 2.6692312640651806E-5 5.338462528130361E-5 0.0 1.4042291833994103 0.0014814233515561752 62-63 2.6692312640651806E-5 5.338462528130361E-5 0.0 1.577609100156764 0.0015748464457984565 64-65 2.6692312640651806E-5 5.338462528130361E-5 0.0 1.7695668665120114 0.0016015387584391084 66-67 2.6692312640651806E-5 5.338462528130361E-5 0.0 1.9890177148871302 0.0016148849147594342 68-69 2.6692312640651806E-5 5.338462528130361E-5 0.0 2.22692629745326 0.00162823107107976 70-71 2.6692312640651806E-5 5.338462528130361E-5 0.0 2.4735232277839216 0.0016816156963610639 72-73 2.6692312640651806E-5 5.338462528130361E-5 0.0 2.7431556239234656 0.0018551157285253005 74-75 2.6692312640651806E-5 5.338462528130361E-5 0.0 3.0262809841028595 0.0019485388227675818 76-77 2.6692312640651806E-5 5.338462528130361E-5 0.0 3.352074006038335 0.0019485388227675818 78-79 2.6692312640651806E-5 5.338462528130361E-5 0.0 3.7049063406787908 0.0020019234480488854 80-81 2.6692312640651806E-5 5.338462528130361E-5 0.0 4.078198332958307 0.002015269604369211 82-83 2.6692312640651806E-5 5.338462528130361E-5 0.0 4.482613561776821 0.0021086926986114926 84-85 2.6692312640651806E-5 5.338462528130361E-5 0.0 4.903244370524533 0.0021353850112521442 86-87 2.6692312640651806E-5 5.338462528130361E-5 0.0 5.366382687152482 0.0022154619491740997 88-89 5.338462528130361E-5 5.338462528130361E-5 0.0 5.859456432406923 0.002295538887096055 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4775 0.0 60.25351 1 GTATCAA 9465 0.0 44.642906 1 TCAACGC 12400 0.0 33.84062 4 ATCAACG 12470 0.0 33.575417 3 CAACGCA 12560 0.0 33.36327 5 TATCAAC 12660 0.0 33.072407 2 AACGCAG 13180 0.0 31.86542 6 ACGCAGA 15250 0.0 27.353367 7 CGCAGAG 15570 0.0 26.699757 8 GTACATG 10415 0.0 26.302658 1 TACATGG 10600 0.0 24.944813 2 ACATGGG 10700 0.0 23.912464 3 GCAGAGT 17165 0.0 23.38936 9 GTGGTAT 2205 0.0 23.038717 1 TATCACG 340 1.8189894E-12 20.94321 2 AGAGTAC 16035 0.0 20.74631 10-11 CATGGGG 7615 0.0 20.695498 4 CAGAGTA 16550 0.0 20.129402 10-11 ATAACGC 310 2.6193447E-10 19.906776 3 GAGTACT 12150 0.0 19.665953 12-13 >>END_MODULE