FastQCFastQC Report
Sat 18 Jun 2016
SRR1930923_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930923_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3127732
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN56370.18022643883811015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.052.0184861
GTATCAA49900.045.139451
ATCAACG59100.037.420183
TCAACGC59950.036.961714
TATCAAC61800.035.791392
CAACGCA62450.034.1869545
AACGCAG67250.031.8695686
ACGCAGA75550.027.9677567
GTACATG58600.027.6272131
TACATGG59100.026.9641132
CGCAGAG79700.026.508618
ACATGGG60050.025.528633
GCAGAGT87000.022.7362779
GTGGTAT13600.021.4421271
CATGGGG46950.019.4777344
AGAGTAC78650.019.07788510-11
CAGAGTA84050.018.82785410-11
TGGTATC13900.018.8062782
GAGTACT59250.018.55110212-13
GTACTTT65850.017.25609814-15
Produced by FastQC (version 0.11.5)