FastQCFastQC Report
Sat 18 Jun 2016
SRR1930923_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930923_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3127732
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52990.1694198863585499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46050.14723128452181963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.054.646611
GTATCAA69450.042.1198351
TCAACGC84000.034.0809754
CAACGCA84300.034.00355
ATCAACG84200.034.000573
TATCAAC88300.032.798732
AACGCAG89150.032.4719856
ACGCAGA102650.028.0627427
CGCAGAG106500.026.9591488
GTACATG83900.025.3001881
TACATGG84350.024.3156852
ACATGGG82600.023.8533293
GTGGTAT19650.022.7166081
GCAGAGT123100.022.706899
CATGGGG57800.021.355984
AGAGTAC110750.020.11834510-11
TATCACG2151.0849271E-619.8743062
TGGTATC20600.019.8207072
CAGAGTA117950.019.47367110-11
GAGTACT82600.018.44273812-13
Produced by FastQC (version 0.11.5)