##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930923_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3127732 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.244042967875764 31.0 31.0 33.0 30.0 34.0 2 31.3574538995029 31.0 31.0 34.0 30.0 34.0 3 31.442119401534402 31.0 31.0 34.0 28.0 34.0 4 35.310358751964685 37.0 35.0 37.0 33.0 37.0 5 35.09836424604154 37.0 35.0 37.0 32.0 37.0 6 35.138286784161814 37.0 35.0 37.0 32.0 37.0 7 35.01985336339558 36.0 35.0 37.0 32.0 37.0 8 34.99232574913707 36.0 35.0 37.0 32.0 37.0 9 36.504452427509776 38.0 35.0 39.0 32.0 39.0 10-11 36.42838516855025 38.0 35.0 39.0 32.0 39.0 12-13 36.32340622534156 38.0 35.0 39.0 32.0 39.0 14-15 37.34147410967436 39.0 36.0 41.0 32.0 41.0 16-17 37.304622486837104 39.0 36.0 40.5 32.0 41.0 18-19 37.29119646440296 39.0 36.0 40.0 32.0 41.0 20-21 37.25145520779913 39.0 36.0 40.0 31.0 41.0 22-23 37.11936124962113 39.0 36.0 40.0 31.0 41.0 24-25 36.99711899868659 39.0 36.0 40.0 31.0 41.0 26-27 36.71467504249085 39.0 35.5 40.0 30.0 41.0 28-29 36.520552272381394 38.0 35.0 40.0 30.0 41.0 30-31 36.27810950554587 38.0 35.0 40.0 30.0 41.0 32-33 36.03008969438558 38.0 34.5 40.0 29.5 41.0 34-35 36.22409944330269 38.0 35.0 40.0 30.0 41.0 36-37 36.275255520613655 38.0 35.0 40.0 30.0 41.0 38-39 36.230374117731316 38.0 35.0 40.0 30.0 41.0 40-41 36.12790545993071 38.0 35.0 40.0 30.0 41.0 42-43 35.97557463363229 38.0 35.0 40.0 29.0 41.0 44-45 35.784960156432845 38.0 34.5 40.0 28.5 41.0 46-47 35.55820191755559 38.0 34.0 40.0 28.0 41.0 48-49 35.344191733818626 38.0 34.0 40.0 27.0 41.0 50-51 35.06370318812481 38.0 34.0 40.0 26.5 41.0 52-53 34.843042018945354 38.0 33.0 40.0 26.0 41.0 54-55 34.658875824399274 38.0 33.0 40.0 26.0 41.0 56-57 34.396767370094366 37.0 33.0 40.0 25.5 41.0 58-59 34.148906939597126 37.0 33.0 40.0 25.0 41.0 60-61 33.82906399908944 37.0 32.0 39.5 24.5 41.0 62-63 33.516149081826704 36.0 32.0 39.0 23.5 41.0 64-65 33.2004653851417 36.0 31.5 39.0 23.0 40.5 66-67 32.85585689566753 35.0 31.0 39.0 22.5 40.0 68-69 32.48073284411836 35.0 31.0 38.5 22.0 40.0 70-71 32.063677770346054 35.0 31.0 38.0 21.5 40.0 72-73 31.63667459360329 34.5 30.0 37.0 20.0 39.5 74-75 31.112054997039387 34.0 30.0 36.5 18.5 39.0 76-77 29.752140688524463 32.5 28.5 35.0 18.0 37.5 78-79 30.5131381141351 34.0 29.5 36.0 18.0 38.0 80-81 30.518534995965126 34.0 30.0 35.5 18.0 37.0 82-83 30.238654079057923 34.0 30.0 35.0 18.0 37.0 84-85 29.918453850905387 34.0 29.0 35.0 16.0 36.5 86-87 29.579952822044856 34.0 29.0 35.0 12.0 36.0 88-89 29.233171671997475 34.0 29.0 35.0 7.0 36.0 90-91 28.891120147122578 34.0 29.0 35.0 4.5 35.0 92-93 28.528107107642214 33.0 29.0 35.0 2.0 35.0 94-95 28.098241313514073 33.0 27.5 35.0 2.0 35.0 96-97 27.629203525110206 33.0 27.0 35.0 2.0 35.0 98-99 27.127510445268328 33.0 26.5 35.0 2.0 35.0 100-101 25.899534710774454 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43741773082267343 1101 2 0.5556382797239259 1101 3 0.48185416142479554 1101 4 0.3788550531121402 1101 5 0.5685216726244064 1101 6 0.4223362518494369 1101 7 0.5310975218938907 1101 8 0.4686709194621841 1101 9 0.6547756709374539 1101 10-11 0.5972509118924805 1101 12-13 0.6790654211637559 1101 14-15 0.7957221046442058 1101 16-17 0.785077993892827 1101 18-19 0.7423869661281088 1101 20-21 0.7580228180937354 1101 22-23 0.7912323433017079 1101 24-25 0.670517912346547 1101 26-27 0.6759452893772888 1101 28-29 0.6342599461181422 1101 30-31 0.7758844578895179 1101 32-33 0.7745162576232687 1101 34-35 0.7540578321013385 1101 36-37 0.8415886919741098 1101 38-39 0.8806741092728103 1101 40-41 0.912130308911081 1101 42-43 0.8940780431180357 1101 44-45 0.9039676458710275 1101 46-47 0.8822178998898593 1101 48-49 0.8685326359658134 1101 50-51 0.8884286820255269 1101 52-53 0.8483771579738928 1101 54-55 0.8234662235979968 1101 56-57 0.8897880699540011 1101 58-59 0.8391370496739796 1101 60-61 0.7720739612246206 1101 62-63 0.8791428974498459 1101 64-65 0.8947411885648506 1101 66-67 0.9184048339619082 1101 68-69 0.856102681397509 1101 70-71 0.843225071724305 1101 72-73 0.7475409075552584 1101 74-75 0.9013381905292555 1101 76-77 0.91741758954959 1101 78-79 0.9490006373519293 1101 80-81 0.8641469951609331 1101 82-83 1.0097540411608215 1101 84-85 1.0773550667484209 1101 86-87 0.8551246880040146 1101 88-89 0.8670176272739027 1101 90-91 0.8588004767013224 1101 92-93 0.9732769491455286 1101 94-95 0.9298290625152781 1101 96-97 1.1467801108480948 1101 98-99 1.2514020695719132 1101 100-101 1.212061534435339 1102 1 0.45427909942456424 1102 2 0.539221836864197 1102 3 0.4907194643078512 1102 4 0.3089834831894649 1102 5 0.3876564953360031 1102 6 0.3984609562701493 1102 7 0.4677442763362052 1102 8 0.4700215391754128 1102 9 0.687457289539509 1102 10-11 0.6453926025129633 1102 12-13 0.6741729558600014 1102 14-15 0.7581176718782388 1102 16-17 0.7229261051009743 1102 18-19 0.7726211114016124 1102 20-21 0.6925016809238542 1102 22-23 0.7344493396559386 1102 24-25 0.7470013345680044 1102 26-27 0.7822620705886365 1102 28-29 0.6939436041063445 1102 30-31 0.8932128171910065 1102 32-33 0.8485241481360362 1102 34-35 0.8463109706077105 1102 36-37 0.7784455025207819 1102 38-39 0.8972952160651957 1102 40-41 0.9463553292939721 1102 42-43 0.873028006914133 1102 44-45 0.8704217050931007 1102 46-47 0.9086715124683309 1102 48-49 0.8508188270531107 1102 50-51 0.8065774244613308 1102 52-53 0.7278217986467226 1102 54-55 0.7673259381870068 1102 56-57 0.8196026565514671 1102 58-59 0.7712372490125503 1102 60-61 0.8444404364994256 1102 62-63 0.670046981404333 1102 64-65 0.6759097781053853 1102 66-67 0.7435357515959637 1102 68-69 0.8492152080040611 1102 70-71 0.6866905660724072 1102 72-73 0.636837427657607 1102 74-75 0.7877223175791279 1102 76-77 1.1289051007550484 1102 78-79 0.9732524305830417 1102 80-81 0.7802928082636846 1102 82-83 1.0022696591328035 1102 84-85 1.1382273407073882 1102 86-87 1.0553065330295617 1102 88-89 1.1048797294974513 1102 90-91 1.0788761904268256 1102 92-93 1.1361365199114815 1102 94-95 1.0150330101957508 1102 96-97 1.1557785478817983 1102 98-99 1.2640221849682014 1102 100-101 1.503196431969286 1103 1 0.3864119732052629 1103 2 0.46607520610912445 1103 3 0.41288608086582457 1103 4 0.2530292875787268 1103 5 0.30735802734625395 1103 6 0.41125676477117423 1103 7 0.38396100641175224 1103 8 0.3981130426927777 1103 9 0.47483568824111444 1103 10-11 0.4355604216795683 1103 12-13 0.4868317932900723 1103 14-15 0.6269816869726839 1103 16-17 0.6293624296710973 1103 18-19 0.561433693266828 1103 20-21 0.616357409992176 1103 22-23 0.5883810738531139 1103 24-25 0.5354333315891111 1103 26-27 0.7392119050983723 1103 28-29 0.648984922396977 1103 30-31 0.6976047070825828 1103 32-33 0.6783000932002423 1103 34-35 0.6706212304777566 1103 36-37 0.727198125003639 1103 38-39 0.7274777323198194 1103 40-41 0.7735438237240189 1103 42-43 0.7740598293333107 1103 44-45 0.7580218758793151 1103 46-47 0.6734457371706242 1103 48-49 0.5772395071848777 1103 50-51 0.563878107540404 1103 52-53 0.44687715877471845 1103 54-55 0.6568300200170327 1103 56-57 0.6827157945244977 1103 58-59 0.6707635080510173 1103 60-61 0.5867627869282614 1103 62-63 0.5354788251388953 1103 64-65 0.4407497910261995 1103 66-67 0.37318952793838633 1103 68-69 0.5323090831867106 1103 70-71 0.4968415566127149 1103 72-73 0.5625158193198736 1103 74-75 0.578826303095191 1103 76-77 0.8276578877084582 1103 78-79 0.967505171813638 1103 80-81 0.8690749959719462 1103 82-83 0.8216698153140349 1103 84-85 0.881130272164615 1103 86-87 0.8892195702150367 1103 88-89 0.8377210108151125 1103 90-91 0.8471217304790208 1103 92-93 0.7430147439453663 1103 94-95 0.8157299047944697 1103 96-97 0.7358829406269329 1103 98-99 0.9432269896133754 1103 100-101 1.1873284452400839 1104 1 0.3851210907455922 1104 2 0.494388749584207 1104 3 0.4224383925206894 1104 4 0.24631316561691818 1104 5 0.34323720198645447 1104 6 0.3735118388106926 1104 7 0.37845472251139967 1104 8 0.448725633622999 1104 9 0.5139373338521764 1104 10-11 0.6003543708501624 1104 12-13 0.5787692415844248 1104 14-15 0.6896642474475172 1104 16-17 0.7000018198894011 1104 18-19 0.7173777307439408 1104 20-21 0.6710404330575059 1104 22-23 0.5616442992289379 1104 24-25 0.5513426656727844 1104 26-27 0.6173556423469719 1104 28-29 0.7486355208734352 1104 30-31 0.7816115057059179 1104 32-33 0.727590704297775 1104 34-35 0.6333983123967997 1104 36-37 0.599866994438834 1104 38-39 0.5898418520524515 1104 40-41 0.7052322744874928 1104 42-43 0.69149976427925 1104 44-45 0.6912281789480303 1104 46-47 0.6192302219035497 1104 48-49 0.6828087598508077 1104 50-51 0.7146113544567285 1104 52-53 0.7289215902277775 1104 54-55 0.7242706151560938 1104 56-57 0.8343043785559274 1104 58-59 0.6786331467705793 1104 60-61 0.7284436022166929 1104 62-63 0.5544738108582337 1104 64-65 0.6338274611545529 1104 66-67 0.6481478105620582 1104 68-69 0.8410018689148799 1104 70-71 0.7258568626213346 1104 72-73 0.5853406992216357 1104 74-75 0.7135335211005263 1104 76-77 0.9290870464812606 1104 78-79 0.8718409560570599 1104 80-81 0.8597225179484091 1104 82-83 0.9689180620459616 1104 84-85 1.036403963195145 1104 86-87 1.0923596764014114 1104 88-89 1.0932523137586152 1104 90-91 1.0031100236612325 1104 92-93 1.0759944869435643 1104 94-95 1.1069722380171676 1104 96-97 1.1060611517949432 1104 98-99 1.2891076348906978 1104 100-101 1.5848432506401942 1105 1 0.2539566087403031 1105 2 0.35534753925306006 1105 3 0.36637874192278375 1105 4 0.2115583651110029 1105 5 0.19034845442983084 1105 6 0.3030422264443615 1105 7 0.31072689610574855 1105 8 0.3316460192444808 1105 9 0.35885871478812703 1105 10-11 0.41014851682003695 1105 12-13 0.2967650303621667 1105 14-15 0.41173709764011335 1105 16-17 0.4040212105557188 1105 18-19 0.4260912239025032 1105 20-21 0.4331230836841584 1105 22-23 0.5126790489989688 1105 24-25 0.5851011186594519 1105 26-27 0.49092070070042126 1105 28-29 0.6092749655095204 1105 30-31 0.5298401064446807 1105 32-33 0.6848475169918018 1105 34-35 0.6859531175738667 1105 36-37 0.633901180077558 1105 38-39 0.5487951908474358 1105 40-41 0.6160332780663111 1105 42-43 0.5532314048593392 1105 44-45 0.587556609369436 1105 46-47 0.5761006765143293 1105 48-49 0.6397919059787824 1105 50-51 0.7129632017172796 1105 52-53 0.7178223067104739 1105 54-55 0.6703782887791832 1105 56-57 0.7104296767909446 1105 58-59 0.6405752586338664 1105 60-61 0.7711828819252133 1105 62-63 0.670512072974482 1105 64-65 0.696795833869281 1105 66-67 0.704525773149733 1105 68-69 0.6431888209529362 1105 70-71 0.7411858733434258 1105 72-73 0.7707888063275341 1105 74-75 0.9011030943010532 1105 76-77 0.8102378743529393 1105 78-79 0.749368947030959 1105 80-81 0.8001714514887084 1105 82-83 0.756189445137835 1105 84-85 0.781203804376009 1105 86-87 0.9015110001337341 1105 88-89 0.7780772713589386 1105 90-91 0.666312942052631 1105 92-93 0.6708110364721378 1105 94-95 0.48143173195185796 1105 96-97 0.31555917374216946 1105 98-99 0.5232308868022777 1105 100-101 0.7830868978748704 1106 1 0.08305667286195018 1106 2 0.16817485664949317 1106 3 0.13339808361673988 1106 4 -0.04981320838844283 1106 5 0.08425593175824275 1106 6 0.023115540531655654 1106 7 0.01809843453865767 1106 8 0.13790086772846166 1106 9 0.1781480236483688 1106 10-11 0.10050447068680057 1106 12-13 0.14773479872271622 1106 14-15 0.18146295791503064 1106 16-17 0.09920726531389334 1106 18-19 0.12250742492387445 1106 20-21 0.1417082638545395 1106 22-23 0.25825198952041717 1106 24-25 0.22095067503211396 1106 26-27 0.11146027434872252 1106 28-29 0.06823496390646966 1106 30-31 0.1288880592036321 1106 32-33 0.20761785843958336 1106 34-35 0.12745933401019016 1106 36-37 0.23822713703590637 1106 38-39 0.1484805582301263 1106 40-41 0.08875782728254222 1106 42-43 0.1251319622739686 1106 44-45 0.09382989461631297 1106 46-47 -0.022189812441851586 1106 48-49 0.00556310297822904 1106 50-51 -0.10238966593735199 1106 52-53 -0.09556857985718636 1106 54-55 -0.011558478401987315 1106 56-57 0.10769754712208623 1106 58-59 0.17789225967297995 1106 60-61 -0.01811627239898428 1106 62-63 -0.10752213871569438 1106 64-65 -0.09176177971763622 1106 66-67 -0.11674653882212027 1106 68-69 0.11121543966672931 1106 70-71 -0.1116859856977257 1106 72-73 -0.2278809915175195 1106 74-75 0.06783642016783986 1106 76-77 0.06334305103098004 1106 78-79 0.16631262732729013 1106 80-81 0.13278353555676858 1106 82-83 0.26379664493974175 1106 84-85 0.15283017197053184 1106 86-87 0.14879316544163146 1106 88-89 0.24963876587997547 1106 90-91 0.20166438378780427 1106 92-93 0.32923525055256064 1106 94-95 0.3912162052096946 1106 96-97 0.43766488822621596 1106 98-99 0.2533604665621638 1106 100-101 0.43034054479359796 1107 1 0.09822731040195265 1107 2 0.18136845565925697 1107 3 0.17454533700793462 1107 4 0.020111087411109452 1107 5 0.07916626055931175 1107 6 0.13628527489762376 1107 7 0.2183780324563216 1107 8 0.05378515862877009 1107 9 0.18498960697630906 1107 10-11 0.21090130778094363 1107 12-13 0.0745076557902209 1107 14-15 0.02249756805277059 1107 16-17 0.1722341427021945 1107 18-19 0.2699762472299412 1107 20-21 0.23773014425505323 1107 22-23 0.23349991541780213 1107 24-25 0.26450640733645514 1107 26-27 0.2471391354065915 1107 28-29 0.3155625996709901 1107 30-31 0.1425104746418029 1107 32-33 0.10496110895704902 1107 34-35 0.036554698567755395 1107 36-37 0.06500642501562481 1107 38-39 0.1198460098931946 1107 40-41 0.09794055618277042 1107 42-43 0.3693379371873675 1107 44-45 0.2829879281020595 1107 46-47 0.3009481827483853 1107 48-49 0.2851448321784815 1107 50-51 0.29603581015251024 1107 52-53 0.08220331453033936 1107 54-55 0.14579309049599232 1107 56-57 0.16209590415299147 1107 58-59 0.18802370378278965 1107 60-61 0.1786892542362395 1107 62-63 0.1411124042843781 1107 64-65 0.04089757681934003 1107 66-67 0.261414270095905 1107 68-69 0.4855939293594318 1107 70-71 0.263361390023114 1107 72-73 0.1944314986926834 1107 74-75 0.3245115158065346 1107 76-77 0.29231065479926244 1107 78-79 0.34085928137170285 1107 80-81 0.34741776563915394 1107 82-83 0.4404276083322003 1107 84-85 0.445174104271441 1107 86-87 0.5437259357247335 1107 88-89 0.5812715246799378 1107 90-91 0.567130856631703 1107 92-93 0.45973411411342013 1107 94-95 0.5242659972813151 1107 96-97 0.5327484602875252 1107 98-99 0.6905940517921643 1107 100-101 0.9852455474117683 1108 1 0.3148772702044411 1108 2 0.3578254169007735 1108 3 0.3994969484445221 1108 4 0.14127780026521464 1108 5 0.2819297671388554 1108 6 0.18614940082191822 1108 7 0.22803662065052777 1108 8 0.33800256653173477 1108 9 0.3457014312504114 1108 10-11 0.434590522579974 1108 12-13 0.4181708739147467 1108 14-15 0.43426371994594604 1108 16-17 0.505099602649743 1108 18-19 0.6353187415539381 1108 20-21 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-0.7826070748435363 2316 74-75 -0.6357835362481232 2316 76-77 -1.0570004532398656 2316 78-79 -0.8487432563750872 2316 80-81 -0.774856055208577 2316 82-83 -1.0372576822062136 2316 84-85 -0.8714568706144235 2316 86-87 -0.86805762309627 2316 88-89 -0.9932419139644431 2316 90-91 -0.6647615532592717 2316 92-93 -1.0035350814957198 2316 94-95 -0.7947459732720255 2316 96-97 -0.8647793679245019 2316 98-99 -0.763959214374534 2316 100-101 -0.8956827155962337 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 20.0 4 68.0 5 113.0 6 238.0 7 626.0 8 1518.0 9 2591.0 10 4293.0 11 6386.0 12 9112.0 13 11560.0 14 13364.0 15 16238.0 16 18834.0 17 21594.0 18 19915.0 19 17779.0 20 17909.0 21 19288.0 22 21512.0 23 25174.0 24 30700.0 25 38228.0 26 48613.0 27 62513.0 28 78610.0 29 96504.0 30 117795.0 31 141420.0 32 169561.0 33 203326.0 34 249097.0 35 309027.0 36 396750.0 37 497698.0 38 396653.0 39 63082.0 40 17.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.36061748454418 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22.09367682397341 27.429092390268732 28.81317836694448 21.664052418813377 20-21 22.61117640513957 26.715092597447608 28.859553823665202 21.814177173747623 22-23 22.324401402934395 26.581594319202743 28.79215916974931 22.301845108113554 24-25 22.234341459172473 26.755768902439414 28.79805660228959 22.211833036098522 26-27 22.176961424393063 26.930938498744755 28.582708055437966 22.309392021424216 28-29 22.20849910182479 27.16004463939588 28.52128724006112 22.110169018718203 30-31 22.41217454140834 26.86883495176674 28.53440916256063 22.184581344264288 32-33 22.233239185467458 27.05566300692422 28.35454538477184 22.356552422836486 34-35 22.508111637621884 27.032114314694127 28.421541030480956 22.038233017203034 36-37 22.56895775570129 26.825455796441616 28.358909004409615 22.246677443447478 38-39 22.316330848501114 26.953941854254687 28.41696135782431 22.312765939419883 40-41 22.517648977190614 26.879887839471046 28.404526067411418 22.197937115926923 42-43 22.458095699904092 26.947722803872225 28.14355193450518 22.450629561718504 44-45 22.353219545573726 27.078779584710915 28.26865891170471 22.299341958010654 46-47 22.57591393689313 27.059006913729856 27.911596344971855 22.453482804405155 48-49 22.47941228054328 27.211378231287515 27.7676051800683 22.541604308100904 50-51 22.544859012244835 27.308747567450688 27.738445891294077 22.407947529010404 52-53 22.692543422753914 27.231148072726434 27.66280053970953 22.41350796481012 54-55 22.36491964915428 27.239917630529852 27.852076481002786 22.54308623931308 56-57 22.437187026307654 27.381134148472537 27.633151865015837 22.54852696020397 58-59 22.405906742428737 27.235171065059134 27.786038082607988 22.57288410990414 60-61 22.340132011121476 27.360486138224445 27.851493166480303 22.44788868417378 62-63 22.468296449935266 27.2809346336652 27.83758194214177 22.413186974257762 64-65 22.221445221818232 27.248646725778087 27.76595296957019 22.763955082833487 66-67 22.199924786160654 27.33693399585524 27.737103627067377 22.72603759091673 68-69 22.390264487337646 27.219950465333653 27.780251968448493 22.609533078880204 70-71 22.251989574972768 27.118255702466854 27.702007304175513 22.927747418384865 72-73 22.25742223894503 27.277639985256624 27.64892790592909 22.816009869869255 74-75 22.244439006433787 27.477092647195278 27.47154779939254 22.8069205469784 76-77 22.22462187094358 27.280975670447955 27.529587829067196 22.96481462954127 78-79 22.18124502295695 27.351502461483022 27.538985405043576 22.928267110516455 80-81 22.290142086587117 27.411867901245817 27.398375582355822 22.899614429811248 82-83 22.312349651440723 27.3733331372381 27.466483701288986 22.84783351003219 84-85 22.2458673322822 27.431206930646063 27.32872051197489 22.99420522509685 86-87 22.205195330034673 27.500102310556017 27.2857297236464 23.008972635762913 88-89 22.170171610355773 27.331111270971714 27.26531264737149 23.233404471301025 90-91 22.116861885179986 27.35657090711781 27.363861026033078 23.162706181669122 92-93 22.191128818468776 27.228132313998394 27.483525665808695 23.09721320172413 94-95 21.967185544924483 27.107135280774724 27.525880633754802 23.399798540545994 96-97 22.01476980764337 26.896965596796658 27.574293449694537 23.51397114586544 98-99 21.820379751206307 27.07078163985917 27.391205512492757 23.717633096441766 100-101 21.839866714923144 26.97843037702719 27.387560698934564 23.794142209115105 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 260.0 1 203.5 2 229.0 3 353.0 4 317.5 5 279.5 6 448.0 7 813.5 8 1248.0 9 1503.5 10 1434.0 11 1288.5 12 1354.0 13 1533.0 14 1765.5 15 1957.0 16 2151.5 17 2418.5 18 2764.5 19 3175.5 20 3740.0 21 4542.5 22 5605.5 23 7131.5 24 9272.5 25 12284.0 26 16165.0 27 20866.0 28 26292.5 29 32826.0 30 40221.5 31 48108.0 32 57711.0 33 63290.0 34 65545.5 35 69671.5 36 76173.0 37 83837.0 38 89242.0 39 92934.0 40 96476.5 41 100094.0 42 103241.5 43 107100.5 44 110457.5 45 114714.5 46 118285.5 47 119306.0 48 119855.0 49 118947.0 50 116050.0 51 112951.0 52 110936.5 53 108939.5 54 105907.5 55 98079.5 56 88080.0 57 78072.0 58 67892.5 59 58608.5 60 48900.5 61 40403.0 62 33269.5 63 25422.5 64 18444.0 65 13759.0 66 10192.5 67 7947.5 68 6259.0 69 4746.5 70 3602.5 71 2668.5 72 2018.0 73 1568.5 74 1145.0 75 853.5 76 641.0 77 419.5 78 249.5 79 160.0 80 100.0 81 58.0 82 31.0 83 17.5 84 11.5 85 8.5 86 6.0 87 3.5 88 1.5 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018959424912364617 2 0.0034849533144144067 3 0.003932561996999743 4 0.036224331240656174 5 0.0022380434129266828 6 3.1972048756095466E-5 7 0.0 8 0.0 9 3.1972048756095466E-5 10-11 0.0 12-13 0.0 14-15 3.1972048756095466E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.394409751219093E-5 24-25 4.4760868258533656E-4 26-27 0.0017904347303413462 28-29 4.795807313414321E-5 30-31 0.007097794823853195 32-33 0.023531427884486266 34-35 0.01390784120890153 36-37 6.394409751219095E-4 38-39 7.193710970121481E-4 40-41 0.0023019875104388736 42-43 0.00890421557857259 44-45 0.008696397261657968 46-47 0.008296746652206774 48-49 0.010279013675084695 50-51 0.19205609687786548 52-53 0.05134711030228932 54-55 0.019263159375547522 56-57 0.011909588161645564 58-59 0.021149510252157156 60-61 0.05459227325103302 62-63 0.09727495834042046 64-65 0.10168710106876165 66-67 0.10573156523640773 68-69 0.10402106062795663 70-71 0.09412571153794506 72-73 0.07225683018877577 74-75 0.24677625832392291 76-77 0.2947822895312003 78-79 0.007913082067133629 80-81 8.4725929203653E-4 82-83 0.0 84-85 1.5986024378047733E-5 86-87 0.0 88-89 2.2380434129266828E-4 90-91 0.0067461022875361445 92-93 0.019023369009876806 94-95 0.0017744487059632988 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3127732.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.38349811603797 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97348361154198 47.68695198874501 2 12.059238706424209 14.563580354204367 3 3.6293458359424235 6.574577923412388 4 1.6660447931231195 4.02406450507139 5 0.9315966793601299 2.8126533166524816 6 0.6151765514761707 2.228790728225525 7 0.40641675838676933 1.7178605895061618 8 0.28917261500186026 1.396900324253968 9 0.22586974781535576 1.2274924942510657 >10 1.1324416833960622 12.069817587940177 >50 0.04947585052526312 2.007501391891816 >100 0.019405482194101983 2.084806540034456 >500 0.0013778137538642242 0.5658050542640675 >1k 9.008782221186023E-4 0.8696348794940486 >5k 5.29928365952119E-5 0.16956232205316862 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5299 0.1694198863585499 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4605 0.14723128452181963 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.197204875609547E-5 3.197204875609547E-5 2 0.0 0.0 0.0 3.197204875609547E-5 6.394409751219095E-5 3 0.0 0.0 0.0 3.197204875609547E-5 6.394409751219095E-5 4 0.0 0.0 0.0 6.394409751219095E-5 6.394409751219095E-5 5 0.0 0.0 0.0 6.394409751219095E-5 6.394409751219095E-5 6 0.0 0.0 0.0 6.394409751219095E-5 9.591614626828642E-5 7 0.0 0.0 0.0 6.394409751219095E-5 9.591614626828642E-5 8 0.0 0.0 0.0 6.394409751219095E-5 9.591614626828642E-5 9 0.0 0.0 0.0 1.278881950243819E-4 9.591614626828642E-5 10-11 0.0 0.0 0.0 1.278881950243819E-4 9.591614626828642E-5 12-13 0.0 0.0 0.0 1.278881950243819E-4 2.557763900487638E-4 14-15 0.0 0.0 0.0 1.278881950243819E-4 3.197204875609547E-4 16-17 0.0 0.0 0.0 1.278881950243819E-4 3.516925363170502E-4 18-19 3.197204875609547E-5 0.0 3.197204875609547E-5 1.758462681585251E-4 5.595108532316707E-4 20-21 3.197204875609547E-5 0.0 3.197204875609547E-5 2.0781831691462057E-4 6.394409751219095E-4 22-23 6.394409751219095E-5 0.0 3.197204875609547E-5 2.717624144268115E-4 7.513431457682436E-4 24-25 9.591614626828642E-5 0.0 3.197204875609547E-5 4.795807313414321E-4 7.673291701462913E-4 26-27 9.591614626828642E-5 0.0 3.197204875609547E-5 7.673291701462913E-4 7.673291701462913E-4 28-29 1.278881950243819E-4 0.0 3.197204875609547E-5 0.0019662809984998717 7.83315194524339E-4 30-31 1.278881950243819E-4 0.0 3.197204875609547E-5 0.0077532218233531515 7.993012189023868E-4 32-33 1.278881950243819E-4 0.0 3.197204875609547E-5 0.02105359410588887 8.312732676584823E-4 34-35 1.278881950243819E-4 3.197204875609547E-5 3.197204875609547E-5 0.041148026749094874 9.271894139267687E-4 36-37 1.9183229253657284E-4 3.197204875609547E-5 3.197204875609547E-5 0.0723687323594221 9.271894139267687E-4 38-39 1.9183229253657284E-4 7.993012189023868E-5 3.197204875609547E-5 0.1164422015696997 9.591614626828642E-4 40-41 2.0781831691462057E-4 9.591614626828642E-5 3.197204875609547E-5 0.16481591133767215 9.911335114389597E-4 42-43 2.557763900487638E-4 1.5986024378047736E-4 3.197204875609547E-5 0.21613104959120538 0.0010071195358170074 44-45 2.557763900487638E-4 1.5986024378047736E-4 3.197204875609547E-5 0.2788761952750427 0.0010550776089511505 46-47 2.557763900487638E-4 1.5986024378047736E-4 3.197204875609547E-5 0.3525397956090867 0.0011509937552194368 48-49 2.8774843880485925E-4 1.5986024378047736E-4 3.197204875609547E-5 0.4324059734018132 0.0012469099014877233 50-51 3.197204875609547E-4 1.5986024378047736E-4 3.197204875609547E-5 0.5151176635338321 0.001278881950243819 52-53 3.197204875609547E-4 1.5986024378047736E-4 3.197204875609547E-5 0.5993799980305218 0.0013907841208901529 54-55 3.3570651193900243E-4 1.5986024378047736E-4 3.197204875609547E-5 0.6946087452505522 0.0014227561696462485 56-57 3.516925363170502E-4 1.5986024378047736E-4 3.197204875609547E-5 0.8042409004352036 0.0014707142427803916 58-59 3.516925363170502E-4 1.5986024378047736E-4 3.197204875609547E-5 0.9240241810999152 0.001518672315914535 60-61 3.516925363170502E-4 1.9183229253657284E-4 3.197204875609547E-5 1.0519123761242972 0.0015986024378047735 62-63 3.8366458507314567E-4 1.9183229253657284E-4 3.197204875609547E-5 1.1867704777775079 0.0016625465353169646 64-65 3.8366458507314567E-4 1.9183229253657284E-4 3.197204875609547E-5 1.3275913665237302 0.0016625465353169646 66-67 3.8366458507314567E-4 2.238043412926683E-4 3.197204875609547E-5 1.4938939781285607 0.00169451858407306 68-69 3.8366458507314567E-4 2.238043412926683E-4 3.197204875609547E-5 1.6778771326955124 0.0017264906328291554 70-71 3.8366458507314567E-4 2.238043412926683E-4 3.197204875609547E-5 1.8765674296902675 0.0017424766572072031 72-73 3.8366458507314567E-4 2.238043412926683E-4 3.197204875609547E-5 2.0838422217760346 0.001870364852231585 74-75 3.8366458507314567E-4 2.238043412926683E-4 3.197204875609547E-5 2.296312471784667 0.002014239071634015 76-77 3.8366458507314567E-4 2.238043412926683E-4 3.197204875609547E-5 2.5390442659409436 0.0020302250960120625 78-79 3.8366458507314567E-4 2.238043412926683E-4 3.197204875609547E-5 2.8145474100722185 0.0020781831691462057 80-81 3.8366458507314567E-4 2.3979036567071604E-4 3.197204875609547E-5 3.117482572036223 0.002110155217902301 82-83 3.8366458507314567E-4 2.557763900487638E-4 3.197204875609547E-5 3.4317038672111293 0.0022060713641705873 84-85 3.8366458507314567E-4 2.557763900487638E-4 3.197204875609547E-5 3.760120752033742 0.0022060713641705873 86-87 3.8366458507314567E-4 2.557763900487638E-4 3.197204875609547E-5 4.108088544670707 0.002333959559194969 88-89 3.8366458507314567E-4 2.557763900487638E-4 3.197204875609547E-5 4.49538195727767 0.002477833778597399 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3615 0.0 54.64661 1 GTATCAA 6945 0.0 42.119835 1 TCAACGC 8400 0.0 34.080975 4 CAACGCA 8430 0.0 34.0035 5 ATCAACG 8420 0.0 34.00057 3 TATCAAC 8830 0.0 32.79873 2 AACGCAG 8915 0.0 32.471985 6 ACGCAGA 10265 0.0 28.062742 7 CGCAGAG 10650 0.0 26.959148 8 GTACATG 8390 0.0 25.300188 1 TACATGG 8435 0.0 24.315685 2 ACATGGG 8260 0.0 23.853329 3 GTGGTAT 1965 0.0 22.716608 1 GCAGAGT 12310 0.0 22.70689 9 CATGGGG 5780 0.0 21.35598 4 AGAGTAC 11075 0.0 20.118345 10-11 TATCACG 215 1.0849271E-6 19.874306 2 TGGTATC 2060 0.0 19.820707 2 CAGAGTA 11795 0.0 19.473671 10-11 GAGTACT 8260 0.0 18.442738 12-13 >>END_MODULE