FastQCFastQC Report
Sat 18 Jun 2016
SRR1930922_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930922_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137723
Sequences flagged as poor quality0
Sequence length101
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20960.18422761955238665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8350.057.835921
GTATCAA13750.043.9027181
TATCAAC18050.033.159352
ATCAACG18450.032.977913
TCAACGC19050.032.4595224
CAACGCA19650.031.4765435
GTACATG31250.031.0684911
AACGCAG22300.029.234296
TACATGG32950.028.0865672
ACATGGG34550.026.4885033
ACGCAGA25850.024.3554487
GTGGTAT3400.023.6730351
CGCAGAG27650.022.7939918
CATGGGG30800.022.6879924
GTAAGTA2453.8817234E-922.5820222
GTATAAG2253.8944563E-822.3578661
GTATATA1150.002668828721.8718261
GTACTAG3059.640644E-1121.441561
TACTAGG3850.020.9023692
TAAGTAC3502.910383E-1120.1137983
Produced by FastQC (version 0.11.5)