##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930922_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1137723 Sequences flagged as poor quality 0 Sequence length 101 %GC 52 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.408195140644956 30.0 10.0 31.0 2.0 33.0 2 21.33949036804213 28.0 10.0 31.0 2.0 33.0 3 21.783824357950046 28.0 10.0 31.0 2.0 33.0 4 23.698235862332044 32.0 2.0 35.0 2.0 37.0 5 23.649690654051998 32.0 10.0 35.0 2.0 37.0 6 24.32076173198573 32.0 10.0 35.0 2.0 37.0 7 24.337490760053193 32.0 10.0 35.0 2.0 37.0 8 25.03380084607589 32.0 17.0 35.0 2.0 37.0 9 26.121223707352318 33.0 17.0 37.0 2.0 39.0 10-11 26.269018908820513 32.5 17.0 37.0 2.0 39.0 12-13 26.134403541107986 33.0 15.5 37.0 2.0 39.0 14-15 26.339904352816987 33.0 13.5 38.0 2.0 40.0 16-17 25.445238427982908 32.0 10.0 38.0 2.0 40.0 18-19 24.798202638076226 32.0 10.0 38.0 2.0 40.0 20-21 24.22095888014921 31.0 2.0 37.0 2.0 39.5 22-23 24.08690911583927 31.0 8.0 37.0 2.0 39.0 24-25 23.73943393954416 30.0 2.0 37.0 2.0 39.0 26-27 23.321408638130723 30.0 2.0 37.0 2.0 39.0 28-29 23.169259564938038 30.0 2.0 37.0 2.0 39.0 30-31 23.01452682243393 30.0 2.0 37.0 2.0 39.0 32-33 22.622069255873356 30.0 2.0 37.0 2.0 39.0 34-35 19.855527663587708 23.5 2.0 35.0 2.0 38.0 36-37 18.227987831836046 16.5 2.0 34.0 2.0 38.0 38-39 17.281324188752446 15.0 2.0 33.0 2.0 38.0 40-41 18.367267779591344 17.0 2.0 33.5 2.0 38.0 42-43 18.20781508328477 16.5 2.0 33.5 2.0 38.0 44-45 16.37892966917255 11.5 2.0 32.0 2.0 37.5 46-47 16.960058819238075 14.5 2.0 31.5 2.0 37.0 48-49 16.41837468346865 9.0 2.0 32.0 2.0 37.0 50-51 14.610942206494903 7.0 2.0 30.0 2.0 36.5 52-53 17.09426064165003 16.0 2.0 32.0 2.0 37.0 54-55 18.50973215800331 21.5 2.0 34.0 2.0 38.0 56-57 18.633371216016553 22.0 2.0 34.0 2.0 38.0 58-59 18.229611689312776 20.5 2.0 34.0 2.0 38.0 60-61 17.839337870465833 19.0 2.0 34.0 2.0 37.5 62-63 17.309522177190757 16.0 2.0 33.0 2.0 37.0 64-65 16.78285575662969 11.5 2.0 32.5 2.0 36.0 66-67 16.322842642717077 8.0 2.0 32.0 2.0 36.0 68-69 15.83108146710579 6.5 2.0 31.5 2.0 35.0 70-71 15.256476752249888 2.0 2.0 31.0 2.0 35.0 72-73 14.76585996767227 2.0 2.0 30.5 2.0 34.0 74-75 14.240572177937864 2.0 2.0 30.0 2.0 34.0 76-77 13.68671372557292 2.0 2.0 29.5 2.0 34.0 78-79 13.19076787583621 2.0 2.0 29.0 2.0 34.0 80-81 12.69469106276308 2.0 2.0 29.0 2.0 34.0 82-83 12.231488683976679 2.0 2.0 27.5 2.0 33.5 84-85 11.751166584484976 2.0 2.0 26.5 2.0 33.0 86-87 11.221312656947253 2.0 2.0 25.5 2.0 33.0 88-89 10.706056746677355 2.0 2.0 25.0 2.0 32.0 90-91 10.17075773276975 2.0 2.0 23.5 2.0 32.0 92-93 9.58710600031818 2.0 2.0 19.5 2.0 32.0 94-95 8.984228146921527 2.0 2.0 15.5 2.0 31.5 96-97 8.337652926063726 2.0 2.0 2.0 2.0 31.0 98-99 7.633822116631201 2.0 2.0 2.0 2.0 31.0 100-101 6.788900285921969 2.0 2.0 2.0 2.0 29.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.0930282045721 1101 2 8.016957821790662 1101 3 7.843977120599877 1101 4 9.560197858260356 1101 5 9.375252959136649 1101 6 8.79761490893485 1101 7 8.718315361186658 1101 8 7.991419847635754 1101 9 8.312032653296818 1101 10-11 7.743333988069569 1101 12-13 7.387324981548417 1101 14-15 7.831088849339977 1101 16-17 8.565051001355666 1101 18-19 8.832478287685635 1101 20-21 9.416224562902425 1101 22-23 9.158327479381256 1101 24-25 9.08787301435676 1101 26-27 9.372693244964648 1101 28-29 9.162229463574619 1101 30-31 9.182566335040157 1101 32-33 9.069107657935948 1101 34-35 11.74214136028855 1101 36-37 5.797375050222563 1101 38-39 8.683266042599207 1101 40-41 10.554708432733793 1101 42-43 10.757690522088723 1101 44-45 5.10131644324176 1101 46-47 6.637301799558859 1101 48-49 9.599588276286308 1101 50-51 -2.5887734062040817 1101 52-53 6.4409837650032244 1101 54-55 9.196996454760715 1101 56-57 9.754706228195726 1101 58-59 9.91235036141839 1101 60-61 9.469302801776536 1101 62-63 9.690794209811994 1101 64-65 9.57421601394081 1101 66-67 9.746898658680902 1101 68-69 9.677677532235805 1101 70-71 9.152646671788686 1101 72-73 9.241550707116781 1101 74-75 9.247393075742414 1101 76-77 9.065095146204209 1101 78-79 9.154505370781685 1101 80-81 8.83140691994596 1101 82-83 8.352492662668446 1101 84-85 8.141127763293388 1101 86-87 7.862519086238677 1101 88-89 7.6820326360890885 1101 90-91 7.332394408443767 1101 92-93 7.169946172984391 1101 94-95 6.9030187595489085 1101 96-97 6.511231982270354 1101 98-99 5.916751789229384 1101 100-101 5.190435663196351 1102 1 6.6615245752449965 1102 2 7.51333063483127 1102 3 7.2234017786227405 1102 4 9.093274849931628 1102 5 8.952185776457544 1102 6 8.31001169158542 1102 7 8.155229987285711 1102 8 7.363881191289337 1102 9 7.579702571793014 1102 10-11 6.957029124592911 1102 12-13 6.692495568839533 1102 14-15 7.044669131482259 1102 16-17 7.804761368568673 1102 18-19 7.84047386472189 1102 20-21 8.237420189875369 1102 22-23 7.943516971275457 1102 24-25 8.193720243762336 1102 26-27 8.278298994817515 1102 28-29 8.059192242907965 1102 30-31 7.719124627205449 1102 32-33 7.898862433131679 1102 34-35 9.939392702337024 1102 36-37 1.181052173869368 1102 38-39 3.294168648224222 1102 40-41 7.659532673428625 1102 42-43 8.59365421098316 1102 44-45 1.2407525153646013 1102 46-47 1.5512740846973436 1102 48-49 6.768062175449867 1102 50-51 -4.178675851318312 1102 52-53 4.026619476868536 1102 54-55 6.946765854272051 1102 56-57 7.36941044249545 1102 58-59 7.600305989104712 1102 60-61 7.522837070828274 1102 62-63 7.4132685726733705 1102 64-65 7.509757024445346 1102 66-67 7.520503552476267 1102 68-69 7.4090145870413195 1102 70-71 7.323876719406885 1102 72-73 7.379838592119723 1102 74-75 7.3549941529481 1102 76-77 7.0091835341409325 1102 78-79 7.058725734249432 1102 80-81 6.637342965317009 1102 82-83 6.6622638456329 1102 84-85 6.93959613375227 1102 86-87 6.542205198489214 1102 88-89 6.402101477379597 1102 90-91 6.325732854342716 1102 92-93 6.00018462066679 1102 94-95 5.503875530428685 1102 96-97 5.488474763743843 1102 98-99 4.9279294033257965 1102 100-101 4.440451892619762 1103 1 6.749318305117519 1103 2 7.425545829527604 1103 3 7.265219562267848 1103 4 8.86487522421432 1103 5 8.779062184222472 1103 6 8.28898403434653 1103 7 8.02739720899191 1103 8 7.06321589900627 1103 9 7.264311740895053 1103 10-11 6.707214309396694 1103 12-13 6.918635905625294 1103 14-15 7.25650784667387 1103 16-17 7.614820112826909 1103 18-19 8.156019159311615 1103 20-21 8.492918239170567 1103 22-23 8.431811369460888 1103 24-25 8.454543514644286 1103 26-27 8.385289129049898 1103 28-29 8.308581424820193 1103 30-31 8.176344019881672 1103 32-33 8.04300348800199 1103 34-35 9.631519790943578 1103 36-37 -2.8398333767731003 1103 38-39 -1.8734673639214332 1103 40-41 6.211071372075455 1103 42-43 8.476364437783918 1103 44-45 0.9105335133098933 1103 46-47 0.6428514885128003 1103 48-49 6.8545937653343465 1103 50-51 -4.427051064907273 1103 52-53 4.180201009326854 1103 54-55 6.977679187783874 1103 56-57 7.704507751188739 1103 58-59 7.916792482456252 1103 60-61 7.689288861337062 1103 62-63 7.806498463388589 1103 64-65 7.812664443027536 1103 66-67 7.684604062343752 1103 68-69 7.716912899130747 1103 70-71 7.6088193241315505 1103 72-73 7.131711893448179 1103 74-75 6.9418116722886705 1103 76-77 6.95365109093037 1103 78-79 7.093435442052183 1103 80-81 6.749377540469771 1103 82-83 6.761338917046615 1103 84-85 6.690829612144173 1103 86-87 6.492504274521735 1103 88-89 6.253783381031321 1103 90-91 6.210974226172519 1103 92-93 5.675079739820083 1103 94-95 5.520764590486227 1103 96-97 5.192737549733369 1103 98-99 4.617106921924943 1103 100-101 4.408307655110897 1104 1 6.5022064642556785 1104 2 7.4052952467770226 1104 3 6.9892635363118245 1104 4 8.634678900267993 1104 5 8.928516052426335 1104 6 7.976617909480403 1104 7 7.748962524307224 1104 8 6.614562919103292 1104 9 7.224074388157703 1104 10-11 6.963730878413262 1104 12-13 6.462311683676077 1104 14-15 7.005679002095022 1104 16-17 7.505940253947056 1104 18-19 8.029474673392127 1104 20-21 7.924976608728937 1104 22-23 7.88710987163439 1104 24-25 7.98099665047242 1104 26-27 8.213006597392361 1104 28-29 7.6288245263132985 1104 30-31 7.239916989704643 1104 32-33 7.221930557554057 1104 34-35 9.603849966398755 1104 36-37 -3.0815843060240287 1104 38-39 -2.2554849803140495 1104 40-41 4.851585137589222 1104 42-43 7.118233689028166 1104 44-45 0.16988606953744778 1104 46-47 -0.4147177721814579 1104 48-49 5.54878358452417 1104 50-51 -4.980934821916032 1104 52-53 3.170814984315829 1104 54-55 5.8571935360482215 1104 56-57 6.073793448599435 1104 58-59 6.380716087004856 1104 60-61 6.339016386064586 1104 62-63 6.499602515867643 1104 64-65 6.414196919560013 1104 66-67 6.298424504289194 1104 68-69 6.421775322743169 1104 70-71 6.166190725252951 1104 72-73 6.504453734940023 1104 74-75 6.391773872250868 1104 76-77 6.099648815053092 1104 78-79 5.905135144376885 1104 80-81 5.556619003961236 1104 82-83 5.601921195128895 1104 84-85 5.433631854946418 1104 86-87 5.233921500313961 1104 88-89 5.2905429334158764 1104 90-91 4.9893055513788465 1104 92-93 4.761075810191153 1104 94-95 4.305263031234668 1104 96-97 4.127132827878647 1104 98-99 3.962734126927147 1104 100-101 3.533925843229084 1105 1 6.1943444655974815 1105 2 6.915399393788366 1105 3 6.675328697642726 1105 4 8.568561665431421 1105 5 8.263028050036251 1105 6 7.717842462387296 1105 7 7.271848528668087 1105 8 6.733248973781784 1105 9 6.503812893724088 1105 10-11 6.1533929640790355 1105 12-13 5.984912358246806 1105 14-15 5.983830798853727 1105 16-17 6.749786827106785 1105 18-19 7.297267000477685 1105 20-21 7.6324935909290765 1105 22-23 7.385732360994311 1105 24-25 7.397550299024736 1105 26-27 7.436634721278562 1105 28-29 7.3950211686597775 1105 30-31 7.525687714278515 1105 32-33 7.400173227428475 1105 34-35 9.086531332627239 1105 36-37 -3.2849867736844693 1105 38-39 -2.131409763319269 1105 40-41 5.331471189122862 1105 42-43 7.280976825388944 1105 44-45 -2.8966999447821102 1105 46-47 -3.988222460631466 1105 48-49 4.367076663753469 1105 50-51 -8.692488024947668 1105 52-53 0.38124193726884315 1105 54-55 4.784704045094912 1105 56-57 6.045313211372775 1105 58-59 6.427163256114028 1105 60-61 6.418589000694439 1105 62-63 6.390929210942804 1105 64-65 6.00997600419846 1105 66-67 5.971969551671631 1105 68-69 5.828367325384846 1105 70-71 5.699313222475004 1105 72-73 5.73932110081209 1105 74-75 5.755418336263281 1105 76-77 5.584141091821026 1105 78-79 5.516832748523896 1105 80-81 5.356441975007632 1105 82-83 5.165917620480592 1105 84-85 4.806045214277193 1105 86-87 4.549606645606119 1105 88-89 4.270840763992224 1105 90-91 4.006535081013832 1105 92-93 3.8174784010249585 1105 94-95 3.2196110248191694 1105 96-97 3.2071450665938723 1105 98-99 2.7750726256796874 1105 100-101 2.373361499559672 1106 1 5.655505544910646 1106 2 6.762496695505455 1106 3 6.507708092573225 1106 4 7.947138934442048 1106 5 7.971122898768876 1106 6 7.077613517170615 1106 7 6.768169051971437 1106 8 6.4143609934056585 1106 9 6.320656720379851 1106 10-11 5.820604738404459 1106 12-13 5.560954876644189 1106 14-15 5.781868411282183 1106 16-17 6.1966388303774345 1106 18-19 6.476334866381205 1106 20-21 6.925216403684598 1106 22-23 6.663328436219167 1106 24-25 6.856258393873468 1106 26-27 6.883077893805137 1106 28-29 6.864767693598452 1106 30-31 6.60009490969961 1106 32-33 6.1638690573548836 1106 34-35 8.358812608179157 1106 36-37 -0.4517897172658252 1106 38-39 0.09262948443216246 1106 40-41 5.083719758215256 1106 42-43 6.054893423917154 1106 44-45 -3.4567980641606884 1106 46-47 -4.683234725553788 1106 48-49 2.9443456743830687 1106 50-51 -8.83312580090842 1106 52-53 -0.48834105537220296 1106 54-55 3.6412044539256563 1106 56-57 4.39854297425094 1106 58-59 4.837220492418197 1106 60-61 4.800436915657642 1106 62-63 5.083079660656622 1106 64-65 4.87833250460729 1106 66-67 5.048829731557156 1106 68-69 4.599830939448623 1106 70-71 4.275355740872383 1106 72-73 4.2139735946796275 1106 74-75 4.084935915985275 1106 76-77 4.434917870234761 1106 78-79 4.285843378123239 1106 80-81 4.161838540829381 1106 82-83 3.9966616924348024 1106 84-85 3.798686260883896 1106 86-87 3.627693317723862 1106 88-89 3.791691131939894 1106 90-91 3.236706789926343 1106 92-93 3.21363539203068 1106 94-95 2.9645006405182706 1106 96-97 2.8371532432087516 1106 98-99 2.5935517753117416 1106 100-101 2.3680466998867358 1107 1 5.783536015075821 1107 2 6.879727356567418 1107 3 6.375618774348407 1107 4 8.012608808296978 1107 5 7.983596655370231 1107 6 6.216056896567508 1107 7 6.618666620917846 1107 8 6.438160900052154 1107 9 6.323349962463297 1107 10-11 5.742819533953085 1107 12-13 5.402451295365431 1107 14-15 5.726662840447755 1107 16-17 6.460216495453075 1107 18-19 6.490242967514604 1107 20-21 6.844246640138682 1107 22-23 6.760102175734083 1107 24-25 6.713680554005499 1107 26-27 6.9810771435729 1107 28-29 7.029575255101456 1107 30-31 6.98255918038026 1107 32-33 6.825998106464983 1107 34-35 8.619676409604601 1107 36-37 -0.007500680148972094 1107 38-39 0.3371092874078201 1107 40-41 5.948049488557174 1107 42-43 6.95920935417567 1107 44-45 -2.8924699049857594 1107 46-47 -4.269276739868429 1107 48-49 3.762305684255235 1107 50-51 -8.706724107190404 1107 52-53 -0.1518864460592404 1107 54-55 4.119296166870829 1107 56-57 5.534588687354539 1107 58-59 5.882019373223578 1107 60-61 5.77742095587428 1107 62-63 5.544387382222265 1107 64-65 5.456674491361042 1107 66-67 5.342147623810451 1107 68-69 5.205561967443543 1107 70-71 5.267295326882975 1107 72-73 5.334261812171038 1107 74-75 5.299356348488269 1107 76-77 5.195244201618758 1107 78-79 5.034515251026306 1107 80-81 4.935652358850275 1107 82-83 4.440039125370522 1107 84-85 4.534081445387999 1107 86-87 4.59635279150076 1107 88-89 4.182571620468082 1107 90-91 4.011689163396666 1107 92-93 3.8117089449040495 1107 94-95 3.6629972955692125 1107 96-97 3.5075377928762865 1107 98-99 3.3194197959752056 1107 100-101 3.202664680452709 1108 1 5.862147712064573 1108 2 6.328711026810449 1108 3 6.767941535578057 1108 4 7.922711134991403 1108 5 7.938020607886777 1108 6 7.477089251863511 1108 7 7.3321136901789785 1108 8 6.528504717236267 1108 9 5.007819954991504 1108 10-11 5.871375797087598 1108 12-13 5.50034328145032 1108 14-15 6.163936665867396 1108 16-17 6.075821220886841 1108 18-19 6.6832870689324615 1108 20-21 7.622657604204054 1108 22-23 7.787486563371374 1108 24-25 7.276483101289752 1108 26-27 7.824454229979704 1108 28-29 7.383098455219578 1108 30-31 7.427596609369555 1108 32-33 7.133539210890653 1108 34-35 9.362333492345513 1108 36-37 -3.0559050634330216 1108 38-39 -2.325435374712974 1108 40-41 4.946449798630351 1108 42-43 7.48491576986466 1108 44-45 -2.469085410426679 1108 46-47 -3.969520241505986 1108 48-49 4.601956100931979 1108 50-51 -8.750098711484332 1108 52-53 0.6584613552342553 1108 54-55 5.173299942228155 1108 56-57 6.202436160881852 1108 58-59 6.697312307093725 1108 60-61 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-12.009816311528157 2313 76-77 -11.487550274305331 2313 78-79 -10.984948560366155 2313 80-81 -10.50539885121649 2313 82-83 -10.060457752818726 2313 84-85 -9.579078610553749 2313 86-87 -9.06278636531826 2313 88-89 -8.537729942670842 2313 90-91 -8.02566604265659 2313 92-93 -7.471529491338505 2313 94-95 -6.8846408149191785 2313 96-97 -6.24706589007007 2313 98-99 -5.536865232047212 2313 100-101 -4.711266464463223 2314 1 -20.117184561385347 2314 2 -19.02899962501785 2314 3 -19.45304415599587 2314 4 -21.33086597152688 2314 5 -21.275810870650584 2314 6 -21.964087218724725 2314 7 -21.990620809026108 2314 8 -22.711558875768503 2314 9 -23.78073330414999 2314 10-11 -23.954538352355964 2314 12-13 -23.803313316323923 2314 14-15 -23.985506895340876 2314 16-17 -23.10303410502731 2314 18-19 -22.470925967633516 2314 20-21 -21.90675416050183 2314 22-23 -21.74550230785279 2314 24-25 -21.39680783670707 2314 26-27 -20.966881223120456 2314 28-29 -20.833515217276446 2314 30-31 -20.699986856449204 2314 32-33 -20.297700852702352 2314 34-35 -17.628922469498683 2314 36-37 -16.05113558807531 2314 38-39 -15.105244595698665 2314 40-41 -16.16444050343642 2314 42-43 -16.003160541741064 2314 44-45 -14.212726109949731 2314 46-47 -14.7588684132071 2314 48-49 -14.262113851373268 2314 50-51 -12.491752129608338 2314 52-53 -14.883271554145711 2314 54-55 -16.27662056651588 2314 56-57 -16.39295334627236 2314 58-59 -15.991479425815655 2314 60-61 -15.593675921627721 2314 62-63 -15.06409940720928 2314 64-65 -14.531315900952809 2314 66-67 -14.091399213659525 2314 68-69 -13.6287054464876 2314 70-71 -13.05416183884961 2314 72-73 -12.565257803521517 2314 74-75 -12.037713307236311 2314 76-77 -11.501713364434087 2314 78-79 -10.99911165049491 2314 80-81 -10.530720739628508 2314 82-83 -10.088783933076236 2314 84-85 -9.604829683515122 2314 86-87 -9.089395807378345 2314 88-89 -8.565626938378996 2314 90-91 -8.053563038364745 2314 92-93 -7.498138933398591 2314 94-95 -6.8846408149191785 2314 96-97 -6.24706589007007 2314 98-99 -5.536865232047212 2314 100-101 -4.711266464463223 2315 1 -20.117184561385347 2315 2 -19.02899962501785 2315 3 -19.45304415599587 2315 4 -17.671166974871362 2315 5 -21.275810870650584 2315 6 -21.964087218724725 2315 7 -21.990620809026108 2315 8 -22.711558875768503 2315 9 -20.316686481407515 2315 10-11 -19.156043369078372 2315 12-13 -16.460922011976095 2315 14-15 -16.517697530792383 2315 16-17 -20.164488954525638 2315 18-19 -22.470925967633516 2315 20-21 -21.90675416050183 2315 22-23 -21.74550230785279 2315 24-25 -21.39680783670707 2315 26-27 -20.966881223120456 2315 28-29 -20.833515217276446 2315 30-31 -20.699986856449204 2315 32-33 -20.297700852702352 2315 34-35 -17.628922469498683 2315 36-37 -16.05113558807531 2315 38-39 -15.105244595698665 2315 40-41 -16.16444050343642 2315 42-43 -16.003160541741064 2315 44-45 -14.212726109949731 2315 46-47 -14.7588684132071 2315 48-49 -14.262113851373268 2315 50-51 -12.491752129608338 2315 52-53 -14.883271554145711 2315 54-55 -16.27662056651588 2315 56-57 -16.39295334627236 2315 58-59 -15.991479425815655 2315 60-61 -15.593675921627721 2315 62-63 -15.06409940720928 2315 64-65 -14.531315900952809 2315 66-67 -14.091399213659525 2315 68-69 -13.6287054464876 2315 70-71 -13.05416183884961 2315 72-73 -12.565257803521517 2315 74-75 -12.037713307236311 2315 76-77 -11.501713364434087 2315 78-79 -10.99911165049491 2315 80-81 -10.530720739628508 2315 82-83 -10.088783933076236 2315 84-85 -9.604829683515122 2315 86-87 -9.089395807378345 2315 88-89 -8.565626938378996 2315 90-91 -8.053563038364745 2315 92-93 -7.498138933398591 2315 94-95 -6.8846408149191785 2315 96-97 -6.24706589007007 2315 98-99 -5.536865232047212 2315 100-101 -4.711266464463223 2316 1 -20.117184561385347 2316 2 -19.02899962501785 2316 3 -19.45304415599587 2316 4 -21.33086597152688 2316 5 -21.275810870650584 2316 6 -21.964087218724725 2316 7 -21.990620809026108 2316 8 -22.711558875768503 2316 9 -23.78073330414999 2316 10-11 -23.954538352355964 2316 12-13 -23.803313316323923 2316 14-15 -23.985506895340876 2316 16-17 -23.10303410502731 2316 18-19 -22.470925967633516 2316 20-21 -21.90675416050183 2316 22-23 -21.74550230785279 2316 24-25 -21.39680783670707 2316 26-27 -20.966881223120456 2316 28-29 -20.833515217276446 2316 30-31 -20.699986856449204 2316 32-33 -20.297700852702352 2316 34-35 -17.628922469498683 2316 36-37 -16.05113558807531 2316 38-39 -15.105244595698665 2316 40-41 -16.16444050343642 2316 42-43 -16.003160541741064 2316 44-45 -14.212726109949731 2316 46-47 -14.7588684132071 2316 48-49 -14.262113851373268 2316 50-51 -12.491752129608338 2316 52-53 -14.883271554145711 2316 54-55 -16.27662056651588 2316 56-57 -16.39295334627236 2316 58-59 -15.991479425815655 2316 60-61 -15.593675921627721 2316 62-63 -15.06409940720928 2316 64-65 -14.531315900952809 2316 66-67 -14.091399213659525 2316 68-69 -13.6287054464876 2316 70-71 -13.05416183884961 2316 72-73 -12.565257803521517 2316 74-75 -12.037713307236311 2316 76-77 -11.501713364434087 2316 78-79 -10.99911165049491 2316 80-81 -10.530720739628508 2316 82-83 -10.088783933076236 2316 84-85 -9.604829683515122 2316 86-87 -9.089395807378345 2316 88-89 -8.565626938378996 2316 90-91 -8.053563038364745 2316 92-93 -7.498138933398591 2316 94-95 -6.8846408149191785 2316 96-97 -6.24706589007007 2316 98-99 -5.536865232047212 2316 100-101 -4.711266464463223 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 203347.0 3 13343.0 4 25883.0 5 18681.0 6 17565.0 7 21525.0 8 27056.0 9 31291.0 10 32365.0 11 29370.0 12 25135.0 13 21242.0 14 20212.0 15 20294.0 16 22295.0 17 23882.0 18 25282.0 19 26286.0 20 27445.0 21 28710.0 22 29842.0 23 31308.0 24 32504.0 25 33963.0 26 35320.0 27 36703.0 28 37928.0 29 39089.0 30 38576.0 31 37124.0 32 35025.0 33 31560.0 34 26304.0 35 18871.0 36 9837.0 37 2427.0 38 132.0 39 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.22623222636972 16.243902739329666 12.261521950138413 26.268343084162204 2 17.670154001323233 18.51853917979763 36.5367944116364 27.274512407242735 3 20.859326221926356 21.828408244087402 26.96204128403898 30.35022424994726 4 14.861221790806898 14.739709614156451 32.86390071042236 37.535167884614296 5 16.374513815153282 35.423030182671226 31.820607805812667 16.381848196362824 6 36.4500284738041 31.607178748492565 15.996310967439367 15.946481810263968 7 31.609588722938426 29.525384433550826 18.93166852986747 19.933358313643275 8 29.04307724557174 32.15865363019749 18.3260215311363 20.472247593094465 9 28.47498323759129 14.459978616602939 17.571625319391842 39.49341282641393 10-11 28.32273716893347 23.555895843657453 25.086581662490048 23.03478532491903 12-13 28.386521963711296 20.95464413221499 25.1231897326858 25.535644171387915 14-15 25.468518684537944 21.930549804168226 22.9620047936924 29.63892671760143 16-17 25.99521511167715 24.75217571569987 23.46658414122959 25.78602503139339 18-19 26.03496597234955 24.17634904958315 24.747354132545286 25.041330845522015 20-21 26.64806839046472 23.45895942541441 24.754284982975662 25.13868720114521 22-23 26.56093714709773 23.04809755428645 24.590330881166715 25.800634417449103 24-25 26.317848887837147 23.371870598650936 24.565871945846478 25.744408567665438 26-27 26.040617559587602 23.766300507807752 24.499290662823014 25.69379126978163 28-29 25.962357323905007 24.10640771056328 24.418902665439692 25.512332300092023 30-31 26.282663073968443 23.847400686573597 24.354407314347675 25.51552892511028 32-33 26.080212297216104 23.915331464049668 24.239134788704185 25.765321450030044 34-35 26.300113415679593 23.850573620566188 24.488448594031045 25.360864369723174 36-37 26.72205123110715 23.993637551843612 24.018761598212784 25.265549618836452 38-39 26.494981228015817 24.004048278627277 24.04551067087062 25.455459822486286 40-41 26.527643610634716 23.827923874857817 24.214968177607474 25.429464336899994 42-43 26.51922931709723 23.819632738332086 24.051360294846134 25.609777649724546 44-45 26.600008026556438 23.993154494011616 24.007774293233037 25.399063186198912 46-47 26.227641891793276 23.8167736077817 24.09051400686188 25.865070493563145 48-49 26.48251872534631 24.14080253240678 23.84090637270956 25.535772369537348 50-51 26.554983124300563 24.17884228689135 23.936630299571533 25.329544289236562 52-53 25.93658517043187 23.937814711263158 24.17378169863588 25.95181841966909 54-55 25.549787117430984 23.700993183324837 24.474946754170503 26.27427294507368 56-57 25.711149977699044 23.923014087189227 24.156497597009167 26.209338338102565 58-59 25.814926934439413 23.972589201410376 24.19356228374667 26.018921580403536 60-61 25.9212741912344 23.992066508433112 24.223577471098885 25.863081829233607 62-63 25.747788189586622 23.954133242076235 24.121012953811157 26.177065614525986 64-65 25.523483338537307 23.891999105561563 24.22237084252943 26.362146713371704 66-67 25.7933664521042 23.958542574209932 23.976581011571582 26.271509962114287 68-69 25.604467838741158 23.922303293236048 24.11181732222947 26.361411545793324 70-71 25.401954230058344 23.829480509718636 24.162951067588644 26.60561419263438 72-73 25.708095884751696 23.929134076169355 23.91875651068692 26.44401352839203 74-75 25.580859282445683 23.934602845148216 24.049016208312455 26.435521664093642 76-77 25.67187564955568 23.690792690531385 23.970658394585403 26.666673265327535 78-79 25.666026459041795 23.801948074553188 23.872417329170492 26.65960813723452 80-81 25.704491917312573 23.913928487813465 23.86779993130424 26.513779663569725 82-83 25.80706032346943 23.897814401057506 23.910671440555504 26.384453834917572 84-85 25.791573898218505 23.769803693091777 23.76618981364591 26.67243259504381 86-87 25.528005908853007 23.879396788284033 23.898424468912488 26.694172833950468 88-89 25.62134849830323 23.636909355546752 23.85665167760989 26.885090468540124 90-91 25.74026398238829 23.70874187980664 23.728601442258444 26.822392695546625 92-93 25.795941036719622 23.49701151515391 23.902443363073235 26.804604085053228 94-95 25.76742428626042 23.400005242357658 23.917762245232545 26.914808226149372 96-97 25.57071331842444 23.473835670991495 23.819204912355662 27.136246098228405 98-99 25.382345352908654 23.401142576325327 23.815910068186007 27.40060200258001 100-101 26.10055373339513 23.29739527825915 23.33292002601868 27.269130962327036 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12085.0 1 13728.5 2 10234.0 3 4845.5 4 6149.5 5 8795.5 6 10049.0 7 9954.0 8 9313.0 9 8534.5 10 7895.5 11 7327.5 12 6554.0 13 5752.0 14 4905.5 15 4079.5 16 3334.0 17 2712.0 18 2323.0 19 2058.5 20 1904.0 21 1876.5 22 2057.5 23 2292.5 24 2571.0 25 2995.0 26 3364.0 27 3673.0 28 4082.5 29 4409.5 30 4714.0 31 5109.0 32 5636.0 33 6083.0 34 6390.0 35 6957.0 36 7755.5 37 8743.5 38 9754.0 39 10942.5 40 12703.0 41 14790.5 42 17241.5 43 20760.5 44 25077.0 45 29635.5 46 34531.5 47 39399.0 48 43823.0 49 47517.5 50 50433.0 51 52358.5 52 53349.5 53 53426.0 54 52286.0 55 50121.5 56 46715.5 57 42447.5 58 38161.0 59 33891.0 60 29269.0 61 24434.0 62 19941.5 63 16057.0 64 12637.0 65 9826.0 66 7619.0 67 5833.5 68 4489.5 69 3411.5 70 2533.0 71 1945.5 72 1538.5 73 1103.0 74 784.0 75 580.0 76 413.5 77 265.0 78 172.5 79 121.0 80 78.5 81 45.0 82 23.0 83 16.5 84 10.0 85 4.0 86 1.5 87 1.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.336090594986652 2 21.22063103233388 3 17.08289276036434 4 22.240738738691228 5 22.10432592116007 6 16.037207650719903 7 14.850714980711475 8 11.946493127061682 9 2.9939625022962533 10-11 6.439660620379477 12-13 3.5180795325399945 14-15 7.350602914769236 16-17 11.386690785015332 18-19 14.430269933894277 20-21 14.838058121352912 22-23 12.827990644471457 24-25 15.314799823858708 26-27 12.859413055726218 28-29 12.89127494126426 30-31 13.753215853067926 32-33 12.22819614264632 34-35 19.053363604321966 36-37 23.90902706546321 38-39 22.83675376168013 40-41 17.974190554291333 42-43 17.937889978492127 44-45 23.346456035432176 46-47 12.680019653289948 48-49 21.365481756104078 50-51 21.14530514017911 52-53 11.143002294934707 54-55 12.758553707712686 56-57 12.305983090787477 58-59 13.287724692214187 60-61 12.697774414334598 62-63 12.673823065895654 64-65 13.720518966391642 66-67 12.048802740210052 68-69 13.085610469332165 70-71 11.715197811769649 72-73 11.06815982449155 74-75 11.155131785153328 76-77 11.199298950623307 78-79 11.630247432810974 80-81 11.587926059330785 82-83 10.102371139548028 84-85 10.010564961770132 86-87 10.15431699983212 88-89 11.562084971473723 90-91 9.049390756801085 92-93 11.060600866819076 94-95 11.138695446958529 96-97 10.723040669829123 98-99 10.572476780376244 100-101 10.681861929485473 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1137723.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 98.23059098723384 #Duplication Level Percentage of deduplicated Percentage of total 1 99.02624719132092 97.2740678485136 2 0.7587728549986608 1.4906941194317826 3 0.12413736368690448 0.3658225979568543 4 0.0390957726735908 0.15361603419317488 5 0.01669526771836947 0.08199930073327602 6 0.01010067817540655 0.059531735192522424 7 0.006959850720400401 0.04785691746134997 8 0.0036020722896938874 0.028306695183629535 9 0.0030168536941120347 0.02667125891651803 >10 0.010467382130149956 0.1934679582643916 >50 4.534396885029715E-4 0.027687648271677443 >100 3.610194554952754E-4 0.06445387200714782 >500 0.0 0.0 >1k 9.025344780877791E-5 0.18582401387405045 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2096 0.18422761955238665 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.394742832833651E-4 2 0.0 0.0 0.0 0.0 4.394742832833651E-4 3 0.0 0.0 0.0 0.0 4.394742832833651E-4 4 0.0 0.0 0.0 0.0 4.394742832833651E-4 5 0.0 0.0 0.0 0.0 4.394742832833651E-4 6 0.0 0.0 0.0 0.0 5.273691399400382E-4 7 0.0 0.0 0.0 0.0 5.273691399400382E-4 8 0.0 0.0 0.0 0.0 5.273691399400382E-4 9 0.0 0.0 0.0 0.0 5.273691399400382E-4 10-11 0.0 0.0 0.0 0.0 5.273691399400382E-4 12-13 0.0 0.0 0.0 0.0 7.031588532533841E-4 14-15 0.0 0.0 0.0 4.394742832833651E-5 7.910537099100572E-4 16-17 0.0 0.0 0.0 8.789485665667302E-5 0.0010547382798800763 18-19 0.0 0.0 0.0 1.318422849850095E-4 0.0010547382798800763 20-21 0.0 0.0 0.0 2.1973714164168255E-4 0.0012305279931934224 22-23 0.0 0.0 0.0 2.636845699700191E-4 0.0013184228498500953 24-25 0.0 0.0 0.0 8.350011382383938E-4 0.0014063177065067683 26-27 0.0 0.0 0.0 0.0014942125631634414 0.0014063177065067683 28-29 0.0 0.0 0.0 0.003691583979580267 0.0014063177065067683 30-31 0.0 0.0 0.0 0.008262116525727264 0.0014063177065067683 32-33 0.0 0.0 0.0 0.022764767874078315 0.001450265134835105 34-35 0.0 0.0 0.0 0.0456174306048133 0.0016700022764767875 36-37 0.0 0.0 0.0 0.07892958127769237 0.0016700022764767875 38-39 0.0 0.0 0.0 0.12305279931934224 0.0017578971331334604 40-41 0.0 0.0 0.0 0.17209812933376578 0.0017578971331334604 42-43 0.0 0.0 0.0 0.21683661137201235 0.00188973941811847 44-45 0.0 0.0 0.0 0.2750230064787299 0.0021094765597601526 46-47 0.0 0.0 0.0 0.34208678210777144 0.0021973714164168255 48-49 0.0 0.0 0.0 0.41209503543481146 0.0021973714164168255 50-51 0.0 0.0 0.0 0.49445251612211405 0.0022852662730734985 52-53 0.0 0.0 0.0 0.5978608149786899 0.0027686879846852003 54-55 0.0 0.0 0.0 0.7188041377382719 0.00290053026967021 56-57 0.0 0.0 0.0 0.8501630010116699 0.00290053026967021 58-59 0.0 0.0 0.0 0.9889929271008848 0.00290053026967021 60-61 0.0 0.0 0.0 1.1309431206014118 0.00290053026967021 62-63 0.0 0.0 0.0 1.2766727929381756 0.0030763199829835558 64-65 0.0 0.0 0.0 1.4314116880822485 0.0030763199829835558 66-67 0.0 0.0 0.0 1.6058390311174162 0.0030763199829835558 68-69 0.0 0.0 0.0 1.7914729683763095 0.0030763199829835558 70-71 0.0 0.0 0.0 1.9800513833332014 0.0032081622679685654 72-73 0.0 0.0 0.0 2.1716621708447486 0.003340004552953575 74-75 0.0 0.0 0.0 2.3627895366446845 0.0034718468379385846 76-77 4.394742832833651E-5 4.394742832833651E-5 0.0 2.573034033767446 0.003515794266266921 78-79 8.789485665667302E-5 8.789485665667302E-5 0.0 2.8078890907540766 0.003559741694595257 80-81 8.789485665667302E-5 8.789485665667302E-5 0.0 3.0499954734148824 0.0036476365512519305 82-83 8.789485665667302E-5 8.789485665667302E-5 0.0 3.306340822854069 0.003867373692893613 84-85 8.789485665667302E-5 8.789485665667302E-5 0.0 3.567388547124388 0.003867373692893613 86-87 8.789485665667302E-5 8.789485665667302E-5 0.0 3.839950497616731 0.003999215977878622 88-89 8.789485665667302E-5 8.789485665667302E-5 0.0 4.119060614929996 0.004131058262863632 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 835 0.0 57.83592 1 GTATCAA 1375 0.0 43.902718 1 TATCAAC 1805 0.0 33.15935 2 ATCAACG 1845 0.0 32.97791 3 TCAACGC 1905 0.0 32.459522 4 CAACGCA 1965 0.0 31.476543 5 GTACATG 3125 0.0 31.068491 1 AACGCAG 2230 0.0 29.23429 6 TACATGG 3295 0.0 28.086567 2 ACATGGG 3455 0.0 26.488503 3 ACGCAGA 2585 0.0 24.355448 7 GTGGTAT 340 0.0 23.673035 1 CGCAGAG 2765 0.0 22.793991 8 CATGGGG 3080 0.0 22.687992 4 GTAAGTA 245 3.8817234E-9 22.582022 2 GTATAAG 225 3.8944563E-8 22.357866 1 GTATATA 115 0.0026688287 21.871826 1 GTACTAG 305 9.640644E-11 21.44156 1 TACTAGG 385 0.0 20.902369 2 TAAGTAC 350 2.910383E-11 20.113798 3 >>END_MODULE