FastQCFastQC Report
Sat 18 Jun 2016
SRR1930922_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930922_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137723
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT13990.12296490446268556No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11650.049.323941
GTATCAA21050.044.6695331
TATAGCG500.001622449637.968295
CAACGCA27650.033.6427845
ATCAACG27900.033.354523
TATCAAC28400.033.2702942
TCAACGC28000.033.2353974
AACGCAG29950.031.5345956
ATAGCGT1056.945137E-527.1202056
ACGCAGA35700.025.7907857
GTACATG42000.024.310141
CGCAGAG38200.023.9786628
GAGTATA1601.8630799E-623.744791
TATAGAG1601.8730807E-623.7301795
TACATGG43150.023.1078952
ACATGGG43700.022.381473
ATACGGG1703.160967E-622.3431243
GCAGAGT40800.021.8689889
GTATACG2005.39736E-721.370311
ATATAAC1856.564447E-620.5360341
Produced by FastQC (version 0.11.5)