FastQCFastQC Report
Sat 18 Jun 2016
SRR1930921_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930921_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466169
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44880.18198266217765288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18650.050.10111
GTATCAA38200.046.1441271
ATCAACG44400.039.1038553
TCAACGC45550.038.7643934
TATCAAC45650.038.38062
CAACGCA47600.036.3076675
AACGCAG51350.034.7134936
ACGCAGA59150.029.9947347
CGCAGAG59750.029.2757648
GTACATG41000.026.6087361
GCAGAGT65450.025.2672799
TACATGG42800.025.1280572
TATAACG2301.7898856E-924.1483762
ATAACGC2752.3646862E-1123.8591383
TATCACG2853.6379788E-1123.0314662
ACATGGG45550.022.6040293
CATGGGG34050.020.1500024
AGAGTAC59350.020.01437410-11
CAGAGTA63600.019.33652910-11
GAGTACT45850.018.9926512-13
Produced by FastQC (version 0.11.5)