FastQCFastQC Report
Sat 18 Jun 2016
SRR1930921_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930921_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466169
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41610.16872323024091213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34050.13806839677248398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27950.052.5034681
GTATCAA52600.041.170911
TCAACGC64300.033.3752334
ATCAACG64650.033.1958923
TATCACG2300.033.0305182
CAACGCA65450.032.774185
TATCAAC68000.031.5611442
AACGCAG69400.031.182326
TATAACG2800.030.5237372
ATAACGC3150.028.6389733
ACGCAGA81900.026.3651667
CGCAGAG84000.025.706048
TAACGCA3350.025.5108244
GTACATG59900.023.3887251
GCAGAGT95200.022.2331499
ACATGGG61150.021.6631773
TACATGG63350.021.4359442
AGAGTAC86250.020.68863310-11
ATCACGC3800.019.991753
CATGGGG41650.019.8349484
Produced by FastQC (version 0.11.5)