##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930921_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2466169 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.263443016273417 31.0 31.0 34.0 30.0 34.0 2 31.38612398420384 31.0 31.0 34.0 30.0 34.0 3 31.470700102061134 31.0 31.0 34.0 30.0 34.0 4 35.33876145552069 37.0 35.0 37.0 33.0 37.0 5 35.11772226477585 37.0 35.0 37.0 32.0 37.0 6 35.157805892459116 37.0 35.0 37.0 32.0 37.0 7 35.042469514457444 37.0 35.0 37.0 32.0 37.0 8 35.01857374737903 37.0 35.0 37.0 32.0 37.0 9 36.52853839294874 38.0 35.0 39.0 32.0 39.0 10-11 36.45591766014414 38.0 35.0 39.0 32.0 39.0 12-13 36.358406702865864 38.0 35.0 39.0 32.0 39.0 14-15 37.38380277264048 39.0 36.0 41.0 32.0 41.0 16-17 37.34508644784684 39.0 36.0 41.0 32.0 41.0 18-19 37.33202651561997 39.0 36.0 41.0 32.0 41.0 20-21 37.30072411906889 39.0 36.0 40.0 31.5 41.0 22-23 37.1677192438961 39.0 36.0 40.0 31.0 41.0 24-25 37.05055513227195 39.0 36.0 40.0 31.0 41.0 26-27 36.784762317586505 39.0 35.5 40.0 30.5 41.0 28-29 36.58918326359629 38.5 35.0 40.0 30.0 41.0 30-31 36.34366582338842 38.0 35.0 40.0 30.0 41.0 32-33 36.104486148353985 38.0 35.0 40.0 30.0 41.0 34-35 36.28701642101575 38.0 35.0 40.0 30.0 41.0 36-37 36.32932495704877 38.0 35.0 40.0 30.0 41.0 38-39 36.287683041997525 38.0 35.0 40.0 30.0 41.0 40-41 36.18935766364754 38.0 35.0 40.0 30.0 41.0 42-43 36.0453726001746 38.0 35.0 40.0 29.5 41.0 44-45 35.862448599426884 38.0 35.0 40.0 29.0 41.0 46-47 35.63954355926135 38.0 34.0 40.0 28.0 41.0 48-49 35.43918016161909 38.0 34.0 40.0 27.0 41.0 50-51 35.16841729013705 38.0 34.0 40.0 26.5 41.0 52-53 34.96172261511681 38.0 33.5 40.0 26.0 41.0 54-55 34.79595275100774 38.0 33.0 40.0 26.0 41.0 56-57 34.553242701534245 38.0 33.0 40.0 26.0 41.0 58-59 34.31928509360064 37.0 33.0 40.0 25.5 41.0 60-61 34.01373567667098 37.0 32.5 40.0 24.5 41.0 62-63 33.711247890959626 36.5 32.0 39.5 24.0 41.0 64-65 33.41046213783403 36.0 32.0 39.0 23.0 41.0 66-67 33.08544669890831 36.0 31.5 39.0 23.0 40.0 68-69 32.725611464583324 35.0 31.0 39.0 22.0 40.0 70-71 32.323432214094005 35.0 31.0 38.0 22.0 40.0 72-73 31.90587404999414 35.0 30.5 37.5 20.5 40.0 74-75 31.373545973532227 34.5 30.0 37.0 19.5 39.0 76-77 29.991712246808714 33.5 28.5 35.5 18.0 38.5 78-79 30.759433355946 34.0 30.0 36.0 19.0 39.0 80-81 30.75965982055569 34.0 30.0 36.0 19.0 37.5 82-83 30.46923730693233 34.0 30.0 35.0 18.0 37.0 84-85 30.1384424587285 34.0 30.0 35.0 17.5 37.0 86-87 29.78715773331025 34.0 29.0 35.0 14.0 36.0 88-89 29.434259371519147 34.0 29.0 35.0 8.5 36.0 90-91 29.092719720343577 34.0 29.0 35.0 6.0 35.5 92-93 28.733800076150498 34.0 29.0 35.0 2.0 35.0 94-95 28.31092861032638 33.0 29.0 35.0 2.0 35.0 96-97 27.85122653800287 33.0 27.5 35.0 2.0 35.0 98-99 27.360125765914663 33.0 27.0 35.0 2.0 35.0 100-101 26.150213144354666 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4766651524613188 1101 2 0.5708115392206814 1101 3 0.6200679882023188 1101 4 0.3521856031369097 1101 5 0.49865550598445196 1101 6 0.4860096284697306 1101 7 0.5722127666007708 1101 8 0.48816783659464136 1101 9 0.7271239045232321 1101 10-11 0.6760794632964533 1101 12-13 0.5745797298075175 1101 14-15 0.7146400622033156 1101 16-17 0.6447084348382006 1101 18-19 0.752965423309341 1101 20-21 0.6535132706138356 1101 22-23 0.7325362604438155 1101 24-25 0.7447500690432491 1101 26-27 0.7577529345341176 1101 28-29 0.6627867366809497 1101 30-31 0.7151987017082391 1101 32-33 0.6820638154818113 1101 34-35 0.8447779407092426 1101 36-37 0.8063202673217091 1101 38-39 0.8668916696714888 1101 40-41 0.9071367038966187 1101 42-43 0.866726528320946 1101 44-45 0.9351710095571306 1101 46-47 0.880254049314523 1101 48-49 1.022244173415963 1101 50-51 0.9753974396142056 1101 52-53 0.969024605566851 1101 54-55 0.9618339860982559 1101 56-57 1.1861428218711438 1101 58-59 1.030011166073443 1101 60-61 0.9961751118708762 1101 62-63 0.9422573417521747 1101 64-65 1.0192492503584774 1101 66-67 1.134749547482123 1101 68-69 0.9811187896599876 1101 70-71 0.9538710381280566 1101 72-73 1.0295273341243067 1101 74-75 1.0315126470428844 1101 76-77 1.0601621661401701 1101 78-79 1.058960999764274 1101 80-81 0.8470570675907361 1101 82-83 1.0027366030820737 1101 84-85 1.0480461230728437 1101 86-87 0.9525876472097998 1101 88-89 0.9825200912273147 1101 90-91 1.0347812477098373 1101 92-93 1.188183621918089 1101 94-95 1.0945853954315155 1101 96-97 1.1255970172069212 1101 98-99 1.3031627872085458 1101 100-101 1.4777479946429786 1102 1 0.4178028186405811 1102 2 0.4379164485468898 1102 3 0.447295476619491 1102 4 0.3118228799538869 1102 5 0.35227489454560157 1102 6 0.40915469533359783 1102 7 0.38331463385904385 1102 8 0.4498982858458902 1102 9 0.6173706593706498 1102 10-11 0.5492933688522825 1102 12-13 0.5619723750620551 1102 14-15 0.6388163088629284 1102 16-17 0.6988907180138284 1102 18-19 0.6261166527417714 1102 20-21 0.7396992429270952 1102 22-23 0.6856118023296744 1102 24-25 0.6525969096064017 1102 26-27 0.8296469999805893 1102 28-29 0.8613453959301225 1102 30-31 0.7857198449207274 1102 32-33 0.7709249027830012 1102 34-35 0.7989060212932344 1102 36-37 0.7260594646191407 1102 38-39 0.7098613694423577 1102 40-41 0.7066480402216015 1102 42-43 0.6729435095587277 1102 44-45 0.7367289489062543 1102 46-47 0.8152592594861261 1102 48-49 0.819521928784404 1102 50-51 0.831267566282996 1102 52-53 0.8328318404223367 1102 54-55 0.7789159958799345 1102 56-57 0.6156920579927601 1102 58-59 0.8304573920604383 1102 60-61 0.65040564515715 1102 62-63 0.5483694192660806 1102 64-65 0.47246345003690493 1102 66-67 0.5010466701228395 1102 68-69 0.5116305069437459 1102 70-71 0.4842784119214585 1102 72-73 0.46286304845003556 1102 74-75 0.6656906724438763 1102 76-77 0.7790296401033139 1102 78-79 0.89505943559233 1102 80-81 0.7394918844223959 1102 82-83 0.8038239462867196 1102 84-85 0.8589781695498147 1102 86-87 0.8951595111626958 1102 88-89 0.8851185942833872 1102 90-91 0.8147418352827351 1102 92-93 0.9621679185729342 1102 94-95 0.9420138536128846 1102 96-97 0.9285761989298678 1102 98-99 0.8740911501502211 1102 100-101 1.0578214692671999 1103 1 0.40579811100604246 1103 2 0.4864783887110633 1103 3 0.45198634217949873 1103 4 0.25165731878509234 1103 5 0.40643236964366736 1103 6 0.4486240837312465 1103 7 0.3856183153004338 1103 8 0.4846713330981345 1103 9 0.5344258074133634 1103 10-11 0.5037347872752989 1103 12-13 0.5701805342318451 1103 14-15 0.5280998866925586 1103 16-17 0.6006860084628372 1103 18-19 0.5806826407324408 1103 20-21 0.6719508515514221 1103 22-23 0.6958324957988751 1103 24-25 0.7228236022638441 1103 26-27 0.7337744620262256 1103 28-29 0.6259401410108225 1103 30-31 0.630714150311924 1103 32-33 0.7439829693127322 1103 34-35 0.6409790163220848 1103 36-37 0.7405979993935574 1103 38-39 0.7085787135644139 1103 40-41 0.7883127174255762 1103 42-43 0.8203245370660142 1103 44-45 0.8708675298124788 1103 46-47 0.7895563483227122 1103 48-49 0.8590406830065902 1103 50-51 0.783356365220186 1103 52-53 0.7218393289698142 1103 54-55 0.7657298451411734 1103 56-57 0.8323789386954914 1103 58-59 0.700054358175457 1103 60-61 0.5842247256557798 1103 62-63 0.6591748196990679 1103 64-65 0.5624337347194341 1103 66-67 0.4281958887225841 1103 68-69 0.7060814631520742 1103 70-71 0.52275748936745 1103 72-73 0.6315010684509836 1103 74-75 0.5500311655614034 1103 76-77 0.7766759083892047 1103 78-79 0.7653565921892778 1103 80-81 0.6152261262009766 1103 82-83 0.74855355845785 1103 84-85 0.8286395764604144 1103 86-87 0.8246986516737458 1103 88-89 0.7441712940549898 1103 90-91 1.0078947355292058 1103 92-93 0.9507108550335559 1103 94-95 0.9706870987390985 1103 96-97 0.8839916726604002 1103 98-99 0.9482257852127205 1103 100-101 1.110024759272683 1104 1 0.42639731957606486 1104 2 0.4958391132346094 1104 3 0.47087821140315 1104 4 0.2473413287322188 1104 5 0.42663360086339 1104 6 0.4470936048588072 1104 7 0.45568214525280126 1104 8 0.47512086750592175 1104 9 0.5408048393904323 1104 10-11 0.4653094961284481 1104 12-13 0.5175755247703862 1104 14-15 0.667724955698688 1104 16-17 0.6088182875470096 1104 18-19 0.6764956878171304 1104 20-21 0.7042742086439446 1104 22-23 0.5380828612031792 1104 24-25 0.6335689246676139 1104 26-27 0.7624531887827359 1104 28-29 0.6575970098934718 1104 30-31 0.6587852597819506 1104 32-33 0.7395283477305696 1104 34-35 0.7195449470499184 1104 36-37 0.7201471653667895 1104 38-39 0.6760317625580754 1104 40-41 0.7834256876725618 1104 42-43 0.7895952361404781 1104 44-45 0.7904171515606038 1104 46-47 0.7923309605383722 1104 48-49 0.7937416822930388 1104 50-51 0.7899993668215117 1104 52-53 0.799473480776868 1104 54-55 0.6831663888086226 1104 56-57 0.6780591752889009 1104 58-59 0.6557045714236196 1104 60-61 0.8205653916254647 1104 62-63 0.651593020904194 1104 64-65 0.6636992366390544 1104 66-67 0.5252267299855973 1104 68-69 0.5485009276463018 1104 70-71 0.5600951959379259 1104 72-73 0.5996582566237585 1104 74-75 0.6913216294130926 1104 76-77 0.8458062254945844 1104 78-79 0.9158712132330997 1104 80-81 0.6777981563858226 1104 82-83 0.8407119844888058 1104 84-85 0.8800165533220792 1104 86-87 0.9747161313013528 1104 88-89 0.9595356637868484 1104 90-91 0.9413793442918745 1104 92-93 1.0605125582775372 1104 94-95 0.8895554526205629 1104 96-97 0.8456481237493989 1104 98-99 0.9981340440023629 1104 100-101 1.3096516018376327 1105 1 0.2823558844515084 1105 2 0.4133123816245501 1105 3 0.3156000478732466 1105 4 0.12049352068842722 1105 5 0.25004645190603725 1105 6 0.3397255437643878 1105 7 0.3191311357068827 1105 8 0.2841337016062795 1105 9 0.45583035093719815 1105 10-11 0.33540366601669547 1105 12-13 0.37396418726024194 1105 14-15 0.4790113742375439 1105 16-17 0.45364601336461163 1105 18-19 0.5025667507811065 1105 20-21 0.48931618598425075 1105 22-23 0.5366633210681613 1105 24-25 0.5685017114042807 1105 26-27 0.511470286486535 1105 28-29 0.6196563116653451 1105 30-31 0.5935599910955176 1105 32-33 0.5182415679221677 1105 34-35 0.539644240113951 1105 36-37 0.5224306535736787 1105 38-39 0.40360105078544706 1105 40-41 0.5202656661334188 1105 42-43 0.5531447903307551 1105 44-45 0.5908696105136855 1105 46-47 0.6462411115583748 1105 48-49 0.6007720498007032 1105 50-51 0.5701744050273163 1105 52-53 0.6578158017281055 1105 54-55 0.6958963905931697 1105 56-57 0.6625163319917462 1105 58-59 0.5660591948483926 1105 60-61 0.5060052793329319 1105 62-63 0.43753155400661115 1105 64-65 0.48593772810203717 1105 66-67 0.5369136438374582 1105 68-69 0.4752412268584507 1105 70-71 0.4636109247104585 1105 72-73 0.3841251613753549 1105 74-75 0.6157269127932281 1105 76-77 0.6101704269222843 1105 78-79 0.5093667576273617 1105 80-81 0.5357067203113779 1105 82-83 0.6320924514546 1105 84-85 0.6928855504471834 1105 86-87 0.6257068662948839 1105 88-89 0.6712884314696659 1105 90-91 0.6770025295742528 1105 92-93 0.6436857599260435 1105 94-95 0.4701954852095618 1105 96-97 0.3565639140000414 1105 98-99 0.47551927162864516 1105 100-101 0.9127934322097495 1106 1 0.04460899377410499 1106 2 0.05623762379785546 1106 3 0.0399383502226236 1106 4 0.0028800395524442024 1106 5 0.10343758854219232 1106 6 0.11004872861527559 1106 7 0.06869417742785089 1106 8 0.09499729100135568 1106 9 0.05794820372791776 1106 10-11 0.17519411547767305 1106 12-13 0.12272139059701459 1106 14-15 0.11870238516586795 1106 16-17 0.005868040560947918 1106 18-19 0.057424069288401824 1106 20-21 8.222853446397949E-4 1106 22-23 0.15391894780323412 1106 24-25 0.15587494170475225 1106 26-27 0.14893632509750887 1106 28-29 0.234441218675002 1106 30-31 0.3260961635452233 1106 32-33 0.22138772672960982 1106 34-35 0.12285278812785805 1106 36-37 0.25665945662026246 1106 38-39 0.14769771113662955 1106 40-41 0.22470479225830076 1106 42-43 0.24030500994502546 1106 44-45 0.09237103023960458 1106 46-47 -0.002678191830227661 1106 48-49 0.0962569869127492 1106 50-51 0.21117695677487092 1106 52-53 0.09919827408466375 1106 54-55 0.04751505277417323 1106 56-57 0.15230771382771735 1106 58-59 0.4015340060720618 1106 60-61 0.19666701102263318 1106 62-63 0.18808624312762845 1106 64-65 0.23337961672081775 1106 66-67 0.08293152945369542 1106 68-69 0.1235983673265153 1106 70-71 0.018061846118939684 1106 72-73 0.21441544966866033 1106 74-75 0.2546373186605031 1106 76-77 0.31621989785127624 1106 78-79 0.3349360465567557 1106 80-81 0.3941255040859204 1106 82-83 0.4094505536008697 1106 84-85 0.38740346669859704 1106 86-87 0.32973345348571215 1106 88-89 0.4053680545178544 1106 90-91 0.4166377755984101 1106 92-93 0.4631300851514659 1106 94-95 0.4419843339454843 1106 96-97 0.4465783554526652 1106 98-99 0.3392093780052292 1106 100-101 0.5612926488667611 1107 1 0.09286738930698846 1107 2 0.09044341910725606 1107 3 0.20387410116392957 1107 4 0.017933307423483313 1107 5 0.07551080737058413 1107 6 0.1351666847983708 1107 7 0.1617910465275756 1107 8 0.15017473050502161 1107 9 0.2409254267027663 1107 10-11 0.09712728028930684 1107 12-13 0.08756190699880761 1107 14-15 0.11998734003719136 1107 16-17 0.09849241275086484 1107 18-19 0.2813762067469341 1107 20-21 0.27557109190087914 1107 22-23 0.22097113259182777 1107 24-25 0.24695220981777766 1107 26-27 0.26319465545840615 1107 28-29 0.3682119836611548 1107 30-31 0.1721830898253316 1107 32-33 0.4721371680551627 1107 34-35 0.3279277717143074 1107 36-37 0.3680093043793775 1107 38-39 0.31017834402384636 1107 40-41 0.24023888619451128 1107 42-43 0.3769541556732392 1107 44-45 0.3682992890325991 1107 46-47 0.42580095846733457 1107 48-49 0.4291366491757316 1107 50-51 0.38911781714154614 1107 52-53 0.36399176729004523 1107 54-55 0.47063344178108935 1107 56-57 0.32577932477842353 1107 58-59 0.3785583963230863 1107 60-61 0.42266739007227017 1107 62-63 0.3260998360169225 1107 64-65 0.23966739839056572 1107 66-67 0.331723838457755 1107 68-69 0.5109074524915442 1107 70-71 0.46819015164287947 1107 72-73 0.4832968046498145 1107 74-75 0.5645327497680839 1107 76-77 0.560900672743287 1107 78-79 0.6071014267585895 1107 80-81 0.7328441965078945 1107 82-83 0.6459874603074596 1107 84-85 0.6957173811663537 1107 86-87 0.6734464974252212 1107 88-89 0.7577361868187431 1107 90-91 0.7144939240713057 1107 92-93 0.6456737536709554 1107 94-95 0.5925573433029285 1107 96-97 0.5574725838009869 1107 98-99 0.5299815944563093 1107 100-101 0.8370685717919706 1108 1 0.19961714949588583 1108 2 0.2638659364035192 1108 3 0.22094680697104607 1108 4 0.09257883206224449 1108 5 0.17608345520138613 1108 6 0.14723966584655557 1108 7 0.20307413255387985 1108 8 0.1949721179402104 1108 9 0.19551577429124478 1108 10-11 0.16203805533302074 1108 12-13 0.19472035625548045 1108 14-15 0.3344621361538316 1108 16-17 0.36927443119318326 1108 18-19 0.451678471219644 1108 20-21 0.34311378647581847 1108 22-23 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68-69 -1.1129821076705753 2316 70-71 -0.9257062337937114 2316 72-73 -0.8413545307766945 2316 74-75 -0.5360932363524675 2316 76-77 -0.9736276861305448 2316 78-79 -1.032577390518135 2316 80-81 -1.040235918087415 2316 82-83 -1.151945182879615 2316 84-85 -1.262119931411391 2316 86-87 -1.1125896440440464 2316 88-89 -1.3390126202575523 2316 90-91 -1.051210246176396 2316 92-93 -1.1727059758136136 2316 94-95 -0.8130807798782023 2316 96-97 -0.882502317447436 2316 98-99 -0.9562575726902836 2316 100-101 -1.0518260552660017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 43.0 5 88.0 6 154.0 7 469.0 8 1183.0 9 2145.0 10 3398.0 11 5165.0 12 7282.0 13 9339.0 14 10515.0 15 12667.0 16 14745.0 17 16571.0 18 15061.0 19 13426.0 20 13495.0 21 14481.0 22 16435.0 23 19042.0 24 23189.0 25 28940.0 26 36352.0 27 47269.0 28 59629.0 29 74170.0 30 89834.0 31 108637.0 32 129739.0 33 156386.0 34 192477.0 35 239260.0 36 306973.0 37 392951.0 38 341614.0 39 63019.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.65051399593054 21.1937797306302 14.35883230636891 23.796873967070358 2 15.377381902407691 21.957093316724084 39.92010043404636 22.74542434682186 3 18.16787993213499 26.93538550374847 30.96945263560837 23.927281928508172 4 12.813383015437635 18.171043056471614 39.03521474692063 29.980359181170122 5 12.614086279801922 38.17610363827168 35.886916917127984 13.32289316479841 6 29.638057763322596 38.99557491443199 18.213932795305425 13.152434526939984 7 25.636361498340136 32.983384350383126 23.88607593396884 17.494178217307898 8 24.425495576337227 36.72363897202503 21.229485894924476 17.621379556713265 9 25.35020917057996 16.210932827393417 21.377569825912175 37.061288176114445 10-11 23.84925769482951 26.768076315937794 29.764484915672853 19.61818107355984 12-13 24.392407819577652 24.48382491224243 29.62315234681808 21.500614921361837 14-15 21.641789683065046 26.179922418115385 27.024633556060746 25.15365434275882 16-17 21.449401886083233 28.90990033529738 27.783760966908595 21.856936811710796 18-19 21.39666827374766 28.038812425263636 29.50391883119121 21.06060046979749 20-21 22.018178802831436 27.355039334287312 29.42057093410873 21.20621092877252 22-23 21.77525582700452 27.250368334523777 29.247008061290213 21.727367777181485 24-25 21.63748120301514 27.390569970604016 29.29554060604905 21.676408220331783 26-27 21.60673283666971 27.53741957262869 29.13725899666379 21.718588594037808 28-29 21.72409213891595 27.627097510611097 29.11264137573786 21.53616897473509 30-31 21.96865276475056 27.365225582737324 29.137231238881245 21.52889041363087 32-33 21.798566476145282 27.554441561443905 28.988614344769687 21.658377617641126 34-35 21.962112738772397 27.549250925241765 28.977241850856622 21.511394485129216 36-37 22.020202716497575 27.40717477642815 28.979076119957657 21.593546387116618 38-39 21.877650257901838 27.51643603403927 28.929285163543277 21.676628544515612 40-41 21.97923133474094 27.396143495103036 28.983049885265068 21.64157528489096 42-43 21.888523491933277 27.544061378037426 28.73122412395804 21.836191006071257 44-45 21.85469229717997 27.615556088139055 28.745477940296848 21.784273674384124 46-47 22.007860181790274 27.59934362636095 28.494791205177183 21.898004986671594 48-49 21.961091081549682 27.70855801959376 28.347969748637286 21.982381150219275 50-51 22.012175500127583 27.835133017928044 28.212290729782396 21.940400752161977 52-53 22.095948070388964 27.789137380191693 28.16090065419139 21.954013895227952 54-55 21.924300231878828 27.718411706078633 28.32764366703133 22.029644395011207 56-57 21.974056257065083 27.886188175776716 28.13471686444673 22.00503870271147 58-59 21.988383642357974 27.81104133942176 28.21760574547758 21.982969272742682 60-61 22.069860746700833 27.90847411279424 28.214362365297802 21.807302775207127 62-63 21.991518759088315 27.866650295443087 28.205473738899954 21.936357206568648 64-65 21.830865998350756 27.736578291010694 28.21016210107708 22.222393609561465 66-67 21.776096210099528 27.82298101599307 28.136769609351468 22.264153164555932 68-69 21.813537997031446 27.854645962422804 28.195341687204955 22.1364743533408 70-71 21.822760138818904 27.641022321139562 28.203246589445385 22.332970950596145 72-73 21.824445976494705 27.78762699436491 28.004963518222226 22.38296351091816 74-75 21.840904046124336 27.877811321797914 27.953427655901773 22.327856976175976 76-77 21.804907940274614 27.761420598169078 27.997460225004378 22.436211236551934 78-79 21.687495970189744 27.78054880034323 28.027933529521732 22.504021699945294 80-81 21.747266689887756 27.894076959664645 27.89105605541271 22.467600295034888 82-83 21.872385874609567 27.780679264073143 27.888640235117705 22.45829462619958 84-85 21.838410473574697 27.860059034895627 27.737419401796753 22.56411108973292 86-87 21.72827571833074 27.896364766567096 27.849693188098623 22.52566632700354 88-89 21.70615812030534 27.686918137927837 27.853026144017445 22.753897597749383 90-91 21.776081300084407 27.715783034596775 27.85434613484173 22.653789530477088 92-93 21.732511856068033 27.618762953870885 27.875036931395915 22.773688258665164 94-95 21.58787526377339 27.538570522583544 27.919024610199294 22.954529603443774 96-97 21.521355592418846 27.424377648084945 28.00150760146608 23.052759158030124 98-99 21.42032845275405 27.442523200964736 27.902264605548122 23.234883740733096 100-101 21.452382217114884 27.394189124913986 27.84204975409228 23.31137890387885 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 320.0 1 239.0 2 273.5 3 385.5 4 309.5 5 274.5 6 404.0 7 694.0 8 1045.0 9 1237.5 10 1168.5 11 1066.0 12 1156.0 13 1297.0 14 1466.5 15 1679.0 16 1866.0 17 2078.0 18 2367.5 19 2696.5 20 3138.0 21 3778.0 22 4724.5 23 5947.5 24 7738.0 25 10563.5 26 14226.0 27 18783.5 28 24460.5 29 31545.5 30 39335.5 31 46794.5 32 54473.0 33 60088.0 34 64281.5 35 67781.5 36 72349.5 37 77817.5 38 80201.0 39 80596.5 40 80550.5 41 81290.5 42 82195.5 43 83815.0 44 85644.5 45 87215.5 46 88650.0 47 88981.0 48 88840.5 49 88235.5 50 85749.0 51 82035.5 52 79606.5 53 77470.0 54 74042.0 55 68346.5 56 61197.0 57 54020.0 58 47097.5 59 40539.5 60 33726.5 61 27313.5 62 22044.5 63 16998.0 64 12633.5 65 9423.5 66 6865.5 67 5348.5 68 4130.0 69 3063.0 70 2336.5 71 1736.0 72 1247.0 73 956.0 74 741.5 75 558.0 76 396.0 77 241.0 78 156.0 79 103.5 80 57.0 81 32.5 82 22.0 83 12.0 84 5.0 85 2.0 86 4.0 87 3.5 88 2.5 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019787776101313412 2 0.002960056670893195 3 0.003933225987351232 4 0.03629110575958095 5 0.002149082240511498 6 8.109744303816972E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.027436075954243E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.027436075954243E-5 24-25 6.285051835458154E-4 26-27 0.0018855155506374462 28-29 6.08230822786273E-5 30-31 0.007217672430397106 32-33 0.022970850740561575 34-35 0.01325943193674075 36-37 6.285051835458154E-4 38-39 7.096026265839852E-4 40-41 0.002027436075954243 42-43 0.00833276227217194 44-45 0.008190841746855143 46-47 0.007927275056981091 48-49 0.00934648031014906 50-51 0.20262196143086705 52-53 0.052470045645695816 54-55 0.017902260550675966 56-57 0.010400747069645267 58-59 0.02059875053169511 60-61 0.055957235696337114 62-63 0.10122988327239536 64-65 0.10597408369012828 66-67 0.11057636358254443 68-69 0.10869084803190698 70-71 0.09664787774073877 72-73 0.07250111407612374 74-75 0.2588224894563187 76-77 0.31155610179188853 78-79 0.006974380101282596 80-81 8.109744303816973E-4 82-83 0.0 84-85 2.027436075954243E-5 86-87 0.0 88-89 1.0137180379771216E-4 90-91 0.005859290259507763 92-93 0.01818610160130956 94-95 0.00150030269620614 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2466169.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.50567061011473 #Duplication Level Percentage of deduplicated Percentage of total 1 81.801527118096 53.58463890802364 2 10.7258634969878 14.052097624854722 3 3.1129903254843305 6.117555566209511 4 1.3933839119597768 3.650981902810809 5 0.7910977519453178 2.591069437966612 6 0.4772779620944728 1.875864778465642 7 0.32799470089379706 1.5039858987028523 8 0.23778879754415475 1.2461211717362126 9 0.17317137267890373 1.020933620802512 >10 0.905347090122184 10.359378211770737 >50 0.03842730825239208 1.6974596168676956 >100 0.014385039502218507 1.6742520007702297 >500 5.588433289561447E-4 0.22786077130813343 >1k 1.8628110964861964E-4 0.3978004897106501 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4161 0.16872323024091213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3405 0.13806839677248398 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.054872151908486E-5 2 0.0 0.0 0.0 0.0 4.054872151908486E-5 3 0.0 0.0 0.0 0.0 4.054872151908486E-5 4 0.0 0.0 0.0 0.0 4.054872151908486E-5 5 0.0 0.0 0.0 4.054872151908486E-5 4.054872151908486E-5 6 0.0 0.0 0.0 8.109744303816972E-5 4.054872151908486E-5 7 0.0 0.0 0.0 8.109744303816972E-5 4.054872151908486E-5 8 0.0 0.0 0.0 8.109744303816972E-5 4.054872151908486E-5 9 0.0 0.0 0.0 1.6219488607633944E-4 4.054872151908486E-5 10-11 0.0 0.0 0.0 1.6219488607633944E-4 6.0823082278627295E-5 12-13 0.0 0.0 0.0 1.8246924683588188E-4 1.4192052531679703E-4 14-15 0.0 0.0 0.0 2.4329232911450918E-4 2.4329232911450918E-4 16-17 0.0 0.0 0.0 3.2438977215267894E-4 3.243897721526789E-4 18-19 0.0 0.0 0.0 3.6493849367176377E-4 3.243897721526789E-4 20-21 0.0 0.0 0.0 4.257615759503911E-4 3.6493849367176377E-4 22-23 0.0 0.0 0.0 5.271333797481032E-4 3.6493849367176377E-4 24-25 0.0 0.0 0.0 7.298769873435275E-4 4.0548721519084865E-4 26-27 0.0 2.027436075954243E-5 0.0 0.0011353642025343762 4.0548721519084865E-4 28-29 0.0 4.054872151908486E-5 0.0 0.0021896309620305825 4.0548721519084865E-4 30-31 0.0 1.2164616455725459E-4 0.0 0.006954105740523054 4.460359367099335E-4 32-33 0.0 1.2164616455725459E-4 0.0 0.018551040094981326 5.879564620267306E-4 34-35 0.0 1.2164616455725459E-4 0.0 0.03691961094312677 6.08230822786273E-4 36-37 0.0 1.2164616455725459E-4 0.0 0.0630735363229365 6.08230822786273E-4 38-39 0.0 1.2164616455725459E-4 0.0 0.10721081969646037 6.893282658244427E-4 40-41 0.0 1.8246924683588188E-4 0.0 0.15641669325986987 7.298769873435275E-4 42-43 0.0 2.4329232911450918E-4 0.0 0.20629162072834425 7.298769873435275E-4 44-45 0.0 2.4329232911450918E-4 0.0 0.26449931046899056 7.298769873435275E-4 46-47 0.0 2.4329232911450918E-4 0.0 0.33114113428560654 7.501513481030699E-4 48-49 0.0 2.4329232911450918E-4 0.0 0.4058116049630013 8.109744303816973E-4 50-51 0.0 2.4329232911450918E-4 0.0 0.48303664509609845 8.109744303816973E-4 52-53 0.0 2.4329232911450918E-4 0.0 0.5614173237924895 8.109744303816973E-4 54-55 0.0 2.4329232911450918E-4 0.0 0.6516990522547319 8.109744303816973E-4 56-57 0.0 2.4329232911450918E-4 0.0 0.7550171946853601 8.312487911412397E-4 58-59 0.0 3.0411541139313645E-4 0.0 0.8677629148691757 8.515231519007822E-4 60-61 0.0 3.243897721526789E-4 0.0 0.9919231001606135 8.515231519007822E-4 62-63 0.0 3.243897721526789E-4 0.0 1.1192866344520591 8.920718734198671E-4 64-65 0.0 3.243897721526789E-4 0.0 1.2497926946612337 9.731693164580367E-4 66-67 0.0 4.0548721519084865E-4 0.0 1.4005528412691912 9.731693164580367E-4 68-69 0.0 4.0548721519084865E-4 0.0 1.571323781946817 9.731693164580367E-4 70-71 0.0 4.0548721519084865E-4 0.0 1.760301098586512 0.001074541120255749 72-73 2.027436075954243E-5 4.0548721519084865E-4 0.0 1.959212851998383 0.0012367360063320885 74-75 4.054872151908486E-5 4.460359367099335E-4 0.0 2.1622403006444406 0.001277284727851173 76-77 4.054872151908486E-5 4.8658465822901836E-4 0.0 2.386778846056373 0.0013381078101298005 78-79 8.109744303816972E-5 4.8658465822901836E-4 0.0 2.644263227702562 0.0013989308924084278 80-81 8.109744303816972E-5 4.8658465822901836E-4 0.0 2.924535990842477 0.0014394796139275126 82-83 8.109744303816972E-5 4.8658465822901836E-4 0.0 3.2254277788748458 0.001459753974687055 84-85 8.109744303816972E-5 4.8658465822901836E-4 0.0 3.53497671895154 0.001459753974687055 86-87 8.109744303816972E-5 4.8658465822901836E-4 0.0 3.866705809699173 0.0015003026962061398 88-89 8.109744303816972E-5 4.8658465822901836E-4 0.0 4.235395060111452 0.0015408514177252248 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2795 0.0 52.503468 1 GTATCAA 5260 0.0 41.17091 1 TCAACGC 6430 0.0 33.375233 4 ATCAACG 6465 0.0 33.195892 3 TATCACG 230 0.0 33.030518 2 CAACGCA 6545 0.0 32.77418 5 TATCAAC 6800 0.0 31.561144 2 AACGCAG 6940 0.0 31.18232 6 TATAACG 280 0.0 30.523737 2 ATAACGC 315 0.0 28.638973 3 ACGCAGA 8190 0.0 26.365166 7 CGCAGAG 8400 0.0 25.70604 8 TAACGCA 335 0.0 25.510824 4 GTACATG 5990 0.0 23.388725 1 GCAGAGT 9520 0.0 22.233149 9 ACATGGG 6115 0.0 21.663177 3 TACATGG 6335 0.0 21.435944 2 AGAGTAC 8625 0.0 20.688633 10-11 ATCACGC 380 0.0 19.99175 3 CATGGGG 4165 0.0 19.834948 4 >>END_MODULE