FastQCFastQC Report
Sat 18 Jun 2016
SRR1930920_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930920_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478472
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63520.18260891563882073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50900.047.453931
GGTATCA26550.046.7223851
ATCAACG62100.038.5496983
TCAACGC63250.038.309084
CAACGCA65100.036.1738435
TATCAAC66100.035.690762
AACGCAG71150.033.7088176
ACGCAGA80700.029.1191067
CGCAGAG85350.027.8338268
GTACATG64250.027.0769921
TACATGG68750.024.9297332
ACATGGG72050.023.8527973
GCAGAGT93200.023.174139
GTGGTAT13300.021.611141
GAGTACA51950.019.8986131
CATGGGG55200.019.7120514
AGAGTAC87200.019.25823210-11
TATACCG3859.094947E-1218.839845
AGTACAT53600.018.2451882
CAGAGTA89150.018.2421410-11
Produced by FastQC (version 0.11.5)