##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930920_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3478472 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.014492857783534 30.0 10.0 31.0 2.0 33.0 2 21.862648599729997 30.0 10.0 31.0 2.0 33.0 3 22.331950063131167 30.0 10.0 31.0 2.0 33.0 4 24.265149180444748 33.0 2.0 35.0 2.0 37.0 5 24.251950569100455 32.0 10.0 35.0 2.0 37.0 6 25.033253681501535 32.0 15.0 35.0 2.0 37.0 7 25.137851332424123 32.0 15.0 35.0 2.0 37.0 8 25.95358565485075 33.0 17.0 35.0 2.0 37.0 9 27.13993356853239 34.0 17.0 37.0 2.0 39.0 10-11 27.41612667861061 34.0 18.5 37.0 2.0 39.0 12-13 27.374987781991635 34.0 18.5 37.0 2.0 39.0 14-15 27.6282780197742 34.0 17.5 38.0 2.0 40.0 16-17 26.733457966601428 33.5 14.0 38.0 2.0 40.0 18-19 26.088248374573663 33.0 10.0 38.0 2.0 40.0 20-21 25.5566576358815 32.0 10.0 38.0 2.0 40.0 22-23 25.490732281300524 32.0 10.0 38.0 2.0 40.0 24-25 25.188340455234368 32.0 10.0 38.0 2.0 40.0 26-27 24.86378659940342 32.0 8.5 38.0 2.0 40.0 28-29 24.79229198912626 32.0 8.5 38.0 2.0 40.0 30-31 24.71173894744589 31.5 8.0 38.0 2.0 40.0 32-33 24.41180940941885 31.0 5.0 37.5 2.0 40.0 34-35 21.476905951808725 26.0 2.0 36.0 2.0 39.0 36-37 19.808586643790722 19.0 2.0 35.0 2.0 38.5 38-39 18.873314921034293 19.0 2.0 34.0 2.0 38.0 40-41 20.23492024659103 24.0 2.0 35.0 2.0 38.0 42-43 20.154495422127876 23.5 2.0 35.0 2.0 38.0 44-45 18.147369592165756 18.5 2.0 34.0 2.0 38.0 46-47 18.999608879990987 19.0 2.0 33.0 2.0 38.0 48-49 18.438210081898028 18.5 2.0 34.0 2.0 38.0 50-51 16.399528586114823 11.0 2.0 32.0 2.0 37.5 52-53 19.349930946691536 23.0 2.0 33.5 2.0 38.0 54-55 21.01423570464273 26.5 2.0 35.5 2.0 38.5 56-57 21.23460128470202 28.0 2.0 36.0 2.0 39.0 58-59 20.850507924169 27.5 2.0 36.0 2.0 39.0 60-61 20.5338289340837 26.5 2.0 35.5 2.0 39.0 62-63 20.050849338445154 26.0 2.0 35.0 2.0 38.5 64-65 19.552394125926554 25.5 2.0 34.0 2.0 38.0 66-67 19.12909217035526 24.0 2.0 34.0 2.0 38.0 68-69 18.668889242173 22.5 2.0 34.0 2.0 37.5 70-71 18.069493875471757 21.5 2.0 33.5 2.0 36.5 72-73 17.59133967443176 19.5 2.0 33.0 2.0 36.0 74-75 17.055972708706584 17.5 2.0 32.0 2.0 36.0 76-77 16.48416732979308 10.5 2.0 32.0 2.0 35.0 78-79 15.978916461020816 7.5 2.0 31.0 2.0 35.0 80-81 15.472633242412186 4.5 2.0 31.0 2.0 34.5 82-83 14.997405182505421 2.0 2.0 31.0 2.0 34.0 84-85 14.475396812163503 2.0 2.0 30.0 2.0 34.0 86-87 13.915875131379526 2.0 2.0 30.0 2.0 34.0 88-89 13.358106519184286 2.0 2.0 29.0 2.0 34.0 90-91 12.78151599322921 2.0 2.0 29.0 2.0 34.0 92-93 12.156360896393588 2.0 2.0 29.0 2.0 34.0 94-95 11.493259684137172 2.0 2.0 27.0 2.0 34.0 96-97 10.763463095290117 2.0 2.0 25.5 2.0 33.0 98-99 9.952481578118208 2.0 2.0 24.5 2.0 32.5 100-101 8.878569958303531 2.0 2.0 10.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.653225181092427 1101 2 7.8502646449598 1101 3 7.441164265743062 1101 4 9.217363454292428 1101 5 9.068296692872035 1101 6 8.446267932565593 1101 7 8.108044274236661 1101 8 7.2776098028224645 1101 9 7.403752438984355 1101 10-11 6.857117583632647 1101 12-13 6.756065682704705 1101 14-15 7.134736837985898 1101 16-17 7.930358997467312 1101 18-19 8.344840688275646 1101 20-21 8.821150624548963 1101 22-23 8.556342012719899 1101 24-25 8.528655870943023 1101 26-27 8.694803521932233 1101 28-29 8.323839445623818 1101 30-31 8.259307624751706 1101 32-33 8.342703846494803 1101 34-35 10.909933669177054 1101 36-37 4.969786901722792 1101 38-39 8.278243039984893 1101 40-41 9.74864052738629 1101 42-43 10.044330795307847 1101 44-45 4.436229534995043 1101 46-47 5.8968512018429955 1101 48-49 9.098551272702608 1101 50-51 -3.708668631228246 1101 52-53 5.784299152861884 1101 54-55 8.173316006125788 1101 56-57 8.716493024938316 1101 58-59 9.073804654201748 1101 60-61 8.811190962741595 1101 62-63 8.974003395570666 1101 64-65 9.090506211144842 1101 66-67 8.930013570546198 1101 68-69 8.764335775131002 1101 70-71 8.835451785177337 1101 72-73 8.652480353778785 1101 74-75 8.747255535433094 1101 76-77 8.445141478316355 1101 78-79 8.418842760154092 1101 80-81 8.279573929119941 1101 82-83 8.001343330845666 1101 84-85 7.9245676127887315 1101 86-87 7.807263429525914 1101 88-89 7.483952338037531 1101 90-91 7.4932395213547025 1101 92-93 7.418383945037776 1101 94-95 7.335943612982659 1101 96-97 7.116168809487693 1101 98-99 6.711977001593194 1101 100-101 6.386110055239648 1102 1 6.689827694350289 1102 2 7.688298400884047 1102 3 7.472337529110725 1102 4 9.18736348706835 1102 5 9.183494151426881 1102 6 8.321556688457061 1102 7 8.087200343354532 1102 8 7.339065971579142 1102 9 7.280704081385913 1102 10-11 6.827450333008269 1102 12-13 6.5269028125825805 1102 14-15 6.863309167895238 1102 16-17 7.816415258979863 1102 18-19 8.109415568151881 1102 20-21 8.515260176436527 1102 22-23 8.371507596688541 1102 24-25 8.388734959247916 1102 26-27 8.438468345499434 1102 28-29 8.386546015885571 1102 30-31 8.029199374068327 1102 32-33 7.975424707059464 1102 34-35 10.4520664231413 1102 36-37 1.056323904102321 1102 38-39 3.5722153017203127 1102 40-41 8.464413817957965 1102 42-43 9.573763616134979 1102 44-45 1.1620767516635695 1102 46-47 1.838370513417484 1102 48-49 7.526266676075906 1102 50-51 -4.945208706350666 1102 52-53 4.74635253992588 1102 54-55 7.66029617996929 1102 56-57 8.07044645690533 1102 58-59 8.401798161291179 1102 60-61 8.30491961742101 1102 62-63 8.417481969949069 1102 64-65 8.292090353497233 1102 66-67 8.179664670833969 1102 68-69 8.121613300352077 1102 70-71 8.10712408657162 1102 72-73 8.029120010352653 1102 74-75 7.941996679378395 1102 76-77 8.034795512049332 1102 78-79 7.938816244431486 1102 80-81 7.813842755610754 1102 82-83 7.663570389337581 1102 84-85 7.379055702017908 1102 86-87 7.337742662700393 1102 88-89 7.439516788614712 1102 90-91 7.30328878356865 1102 92-93 7.445617583296455 1102 94-95 6.976813838349914 1102 96-97 6.699075550512006 1102 98-99 6.524385515795 1102 100-101 6.059411852671326 1103 1 6.522201744288857 1103 2 7.695874087765652 1103 3 7.3348638241632464 1103 4 9.031572912730564 1103 5 9.057241290624862 1103 6 8.337406977239052 1103 7 8.179530901525467 1103 8 7.392726741374656 1103 9 7.159270248810479 1103 10-11 6.683121869269417 1103 12-13 6.598010177749533 1103 14-15 6.997767560598071 1103 16-17 7.742776031686521 1103 18-19 8.048615385028654 1103 20-21 8.612255990363863 1103 22-23 8.508723126591416 1103 24-25 8.54836111189341 1103 26-27 8.443392175087954 1103 28-29 8.330608807374333 1103 30-31 8.14508619178774 1103 32-33 8.017841662547998 1103 34-35 10.309035773528382 1103 36-37 -3.1992537974538635 1103 38-39 -1.7600068899281993 1103 40-41 6.681784763335639 1103 42-43 9.07532782032855 1103 44-45 0.706983616830712 1103 46-47 0.626474402353562 1103 48-49 7.476807810809863 1103 50-51 -5.227648456534208 1103 52-53 4.522667511885135 1103 54-55 7.564546696698226 1103 56-57 8.106351610875201 1103 58-59 8.378231094600746 1103 60-61 8.443836733820309 1103 62-63 8.441565107577429 1103 64-65 8.409000579645653 1103 66-67 8.312745093118057 1103 68-69 8.219039406020404 1103 70-71 8.169333903843192 1103 72-73 8.134211450338519 1103 74-75 7.97391292123665 1103 76-77 7.8871370528287486 1103 78-79 7.892650765952251 1103 80-81 7.755569768799852 1103 82-83 7.555611268249338 1103 84-85 7.319176040141876 1103 86-87 7.175997353139582 1103 88-89 7.117960847298692 1103 90-91 7.033767356515462 1103 92-93 7.144660293839324 1103 94-95 6.822647246455906 1103 96-97 6.466076121974531 1103 98-99 6.0846242858385615 1103 100-101 5.740266063528637 1104 1 6.295876915454588 1104 2 7.39922554589932 1104 3 6.884469288427304 1104 4 8.718805779159357 1104 5 8.699581746278245 1104 6 7.89221424057116 1104 7 7.626488617465327 1104 8 6.7500761336909 1104 9 6.732861501964386 1104 10-11 6.275707722238732 1104 12-13 6.077458530916761 1104 14-15 6.562412953443342 1104 16-17 7.249426487554128 1104 18-19 7.6020054258886205 1104 20-21 7.995925345014797 1104 22-23 7.9076128661475416 1104 24-25 7.843703382820987 1104 26-27 7.792941895811413 1104 28-29 7.606575308160885 1104 30-31 7.355857431846339 1104 32-33 7.173866994424646 1104 34-35 9.53510447080603 1104 36-37 -3.531620816278135 1104 38-39 -2.1735149888080194 1104 40-41 5.938901262212852 1104 42-43 7.894644936343102 1104 44-45 0.07303717440489521 1104 46-47 -0.37349986806734137 1104 48-49 6.57605680980117 1104 50-51 -5.5546881718276815 1104 52-53 3.5166864907677855 1104 54-55 6.2192091758467924 1104 56-57 6.775820356771273 1104 58-59 6.856158724862272 1104 60-61 6.659840625157198 1104 62-63 6.87834555253848 1104 64-65 7.132099198677441 1104 66-67 6.77386292574214 1104 68-69 6.879026278113894 1104 70-71 6.905209179883155 1104 72-73 6.811152834398939 1104 74-75 6.760204085070502 1104 76-77 6.659911688726098 1104 78-79 6.3585910900937215 1104 80-81 6.166261002674041 1104 82-83 6.072816556294887 1104 84-85 6.075911252270032 1104 86-87 6.035336072686704 1104 88-89 5.9464273317859675 1104 90-91 5.890151249286685 1104 92-93 5.742028472241005 1104 94-95 5.42878490366247 1104 96-97 5.16836824327471 1104 98-99 4.793853497502887 1104 100-101 4.222651779956541 1105 1 6.05423138437224 1105 2 6.999013704877729 1105 3 6.543454974451379 1105 4 8.603644439145118 1105 5 8.516693442837965 1105 6 7.627609764072599 1105 7 7.505227371939583 1105 8 6.56011392908874 1105 9 6.613018493567161 1105 10-11 6.172796985361895 1105 12-13 5.833727187542653 1105 14-15 6.182338745406113 1105 16-17 6.799285846463636 1105 18-19 7.410026546917578 1105 20-21 7.861581567003327 1105 22-23 7.559788770004584 1105 24-25 7.576616460683635 1105 26-27 7.6844093057714 1105 28-29 7.624601353750677 1105 30-31 7.281121156633738 1105 32-33 6.938891908335801 1105 34-35 9.483339725324559 1105 36-37 -3.6342563660277243 1105 38-39 -2.355177003826718 1105 40-41 5.457371397662534 1105 42-43 7.662505263183537 1105 44-45 -3.454384502638524 1105 46-47 -4.257939620432282 1105 48-49 4.952339803448968 1105 50-51 -9.872958383450374 1105 52-53 0.4685796872322925 1105 54-55 5.102254950003523 1105 56-57 6.134526701327999 1105 58-59 6.622105629363393 1105 60-61 6.602045415323598 1105 62-63 6.663150431977712 1105 64-65 6.5546786739327345 1105 66-67 6.366657086377433 1105 68-69 6.114784017619179 1105 70-71 6.239757581887428 1105 72-73 6.085574744327996 1105 74-75 5.698825377852625 1105 76-77 5.522251867926741 1105 78-79 5.407805313341289 1105 80-81 5.373042219962407 1105 82-83 5.225426088352444 1105 84-85 5.246128363542059 1105 86-87 5.087958371001072 1105 88-89 5.1817358264107956 1105 90-91 5.1075137527587415 1105 92-93 4.815975726070578 1105 94-95 4.53544622123721 1105 96-97 4.42400010324252 1105 98-99 4.046730605451444 1105 100-101 3.728458607387072 1106 1 5.89867752162117 1106 2 7.169954278028648 1106 3 6.762053614691471 1106 4 7.89651958193873 1106 5 8.103839899968118 1106 6 7.231596750000886 1106 7 7.199805214606023 1106 8 6.433460258454634 1106 9 6.4292453186489595 1106 10-11 5.99224262261259 1106 12-13 5.730278287950544 1106 14-15 6.300789209698593 1106 16-17 6.769281981771396 1106 18-19 6.937693066918101 1106 20-21 7.531654202041082 1106 22-23 7.1600806219157 1106 24-25 7.238809711766276 1106 26-27 7.422396554051705 1106 28-29 7.214262507887778 1106 30-31 6.896398985064934 1106 32-33 6.864398347724837 1106 34-35 9.469630361945843 1106 36-37 -0.08406713140149336 1106 38-39 1.0542231688785577 1106 40-41 6.86837715293634 1106 42-43 7.8806096404622075 1106 44-45 -3.4755404573822126 1106 46-47 -4.348936799265768 1106 48-49 4.7363735068594295 1106 50-51 -9.76964624808144 1106 52-53 0.4380825889867559 1106 54-55 5.078627239277793 1106 56-57 6.144748282898885 1106 58-59 6.508324854472008 1106 60-61 6.522275990980393 1106 62-63 6.569699144158729 1106 64-65 6.477488211075542 1106 66-67 6.563840361024699 1106 68-69 6.230896401928412 1106 70-71 6.121927262465025 1106 72-73 6.089275466560739 1106 74-75 6.02190276699125 1106 76-77 5.82477359432065 1106 78-79 5.549875988385963 1106 80-81 5.467163799533299 1106 82-83 5.434557703182039 1106 84-85 5.262906980794 1106 86-87 5.013127311581282 1106 88-89 5.045560130560313 1106 90-91 4.844786591795781 1106 92-93 4.538928321532007 1106 94-95 4.249374583842988 1106 96-97 3.9580001303012473 1106 98-99 3.551941616471405 1106 100-101 3.1707393993372186 1107 1 5.9112390701871185 1107 2 7.170200644979559 1107 3 6.6221025081699985 1107 4 8.021134921389791 1107 5 8.389902150219374 1107 6 6.887925214708819 1107 7 7.024790277170563 1107 8 6.352105631648758 1107 9 6.28323663594081 1107 10-11 5.940233436809304 1107 12-13 5.5815258947949395 1107 14-15 5.95252736307468 1107 16-17 6.861902140344217 1107 18-19 7.044577612917919 1107 20-21 7.5140468798771565 1107 22-23 7.381149629989391 1107 24-25 7.348337809686779 1107 26-27 7.255942148630712 1107 28-29 7.367291641355344 1107 30-31 7.127478377390489 1107 32-33 7.06443852626029 1107 34-35 9.290293497736627 1107 36-37 -0.1519249650070975 1107 38-39 0.9077312500599213 1107 40-41 7.033316804545841 1107 42-43 8.276257407099745 1107 44-45 -3.203337931084844 1107 46-47 -4.200649366167635 1107 48-49 4.982020496404953 1107 50-51 -9.735279929206623 1107 52-53 0.6432786291560824 1107 54-55 5.429324262036804 1107 56-57 6.596824298615715 1107 58-59 6.924812070444876 1107 60-61 6.9545340016478505 1107 62-63 6.730387958523533 1107 64-65 6.695009362369028 1107 66-67 6.60080634051177 1107 68-69 6.548898141463287 1107 70-71 6.643368958114348 1107 72-73 6.379230660010602 1107 74-75 6.334838298286186 1107 76-77 6.230597380934636 1107 78-79 6.175228557912675 1107 80-81 6.063633729525943 1107 82-83 5.868767797780039 1107 84-85 5.680653661459836 1107 86-87 5.345901426473475 1107 88-89 5.42245623508437 1107 90-91 5.124857462587826 1107 92-93 5.030658191682809 1107 94-95 4.856057517635898 1107 96-97 4.655158880414932 1107 98-99 4.538232274213602 1107 100-101 4.215985006464821 1108 1 5.974096524397556 1108 2 6.430358312499518 1108 3 6.7139452469538945 1108 4 8.19207211756883 1108 5 8.208536132756844 1108 6 7.557551312732183 1108 7 7.641214256950974 1108 8 6.585920270025522 1108 9 5.015756820962707 1108 10-11 5.834931562718548 1108 12-13 5.308484120043762 1108 14-15 5.88807126110494 1108 16-17 5.978444198626061 1108 18-19 6.821549730173572 1108 20-21 7.532515211080806 1108 22-23 7.579206257024364 1108 24-25 7.3099913895566395 1108 26-27 7.724811036254405 1108 28-29 7.529666118929114 1108 30-31 7.431513020200178 1108 32-33 7.2715077083120185 1108 34-35 9.674310391782651 1108 36-37 -3.58977637302921 1108 38-39 -2.1989105304955956 1108 40-41 5.688338512495882 1108 42-43 8.0773169340289 1108 44-45 -3.150199582345822 1108 46-47 -4.260667814866512 1108 48-49 5.009135197007662 1108 50-51 -9.873791808615184 1108 52-53 0.6931940749906289 1108 54-55 5.476903567399475 1108 56-57 6.461905526666271 1108 58-59 6.874853341015079 1108 60-61 6.938092174942135 1108 62-63 6.900655414865074 1108 64-65 6.887875265594165 1108 66-67 6.80601425683594 1108 68-69 6.7273838970703785 1108 70-71 6.776057789111572 1108 72-73 6.619699199772189 1108 74-75 6.503374837607321 1108 76-77 6.502052529171525 1108 78-79 6.3738930758351415 1108 80-81 6.153091211449265 1108 82-83 6.17549210524963 1108 84-85 6.057697806601361 1108 86-87 6.085540998249698 1108 88-89 6.028347470273696 1108 90-91 5.794668894066305 1108 92-93 5.741886333289374 1108 94-95 5.526884886582279 1108 96-97 5.218743015330574 1108 98-99 4.899432311714195 1108 100-101 4.524977986057728 1109 1 4.807120293424266 1109 2 6.502474562833491 1109 3 6.159437462769361 1109 4 7.792418362163712 1109 5 7.670828001513765 1109 6 5.128701813820811 1109 7 4.752216943998665 1109 8 3.4504617647563123 1109 9 2.736716621204124 1109 10-11 2.6258356056850367 1109 12-13 3.290487526369727 1109 14-15 3.892178372877808 1109 16-17 3.105553142652159 1109 18-19 4.1512366806207694 1109 20-21 4.479854281314033 1109 22-23 6.067988864627296 1109 24-25 5.782060344880993 1109 26-27 4.601129980833132 1109 28-29 4.6979355728216845 1109 30-31 4.705794837910759 1109 32-33 4.33019650272195 1109 34-35 6.7792293084013515 1109 36-37 -4.671598310459567 1109 38-39 -3.3251391547434537 1109 40-41 3.2560745480802353 1109 42-43 4.180524084029049 1109 44-45 -4.649160648642658 1109 46-47 -6.031946892122594 1109 48-49 2.1591973573871535 1109 50-51 -10.100016427352717 1109 52-53 -0.9795058231794442 1109 54-55 2.629030421982886 1109 56-57 4.002605795542401 1109 58-59 4.843687222006153 1109 60-61 4.988685304627992 1109 62-63 5.141776069546815 1109 64-65 4.7269592432244245 1109 66-67 4.904758118801912 1109 68-69 4.827800148846617 1109 70-71 4.905005448015739 1109 72-73 4.848935341099157 1109 74-75 4.956771903060503 1109 76-77 5.051568704935885 1109 78-79 5.06561379309138 1109 80-81 4.813715888277407 1109 82-83 4.681604756764662 1109 84-85 4.521205001454657 1109 86-87 4.451687088992998 1109 88-89 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2313 68-69 -16.46300406157507 2313 70-71 -15.778646717126811 2313 72-73 -15.26374635429575 2313 74-75 -14.723019710008701 2313 76-77 -14.225141906776814 2313 78-79 -13.721248153490466 2313 80-81 -13.14795625020284 2313 82-83 -12.67803642771975 2313 84-85 -12.152695836419843 2313 86-87 -11.602367656428804 2313 88-89 -11.04680928288012 2313 90-91 -10.47783153154051 2313 92-93 -9.877918774858038 2313 94-95 -9.358036801763886 2313 96-97 -8.604671202533947 2313 98-99 -7.78314045983738 2313 100-101 -6.713081614766363 2314 1 -20.866360072363793 2314 2 -19.715115539371997 2314 3 -20.15013757757491 2314 4 -22.095424564140753 2314 5 -22.07312035781921 2314 6 -22.841174463747773 2314 7 -22.944663099035225 2314 8 -23.76847314648629 2314 9 -24.933805164702278 2314 10-11 -25.2075425546162 2314 12-13 -25.162713119138612 2314 14-15 -25.413362240355113 2314 16-17 -24.523414044007335 2314 18-19 -23.888166224166753 2314 20-21 -23.369661587432603 2314 22-23 -23.273727111704066 2314 24-25 -22.978832608319653 2314 26-27 -22.66608357484196 2314 28-29 -22.585146729491385 2314 30-31 -22.49256449506396 2314 32-33 -22.191858365486762 2314 34-35 -19.37851679165244 2314 36-37 -17.677246508415458 2314 38-39 -16.784256960015107 2314 40-41 -18.124775371231223 2314 42-43 -18.049707223125335 2314 44-45 -16.055561939659334 2314 46-47 -16.8603492589865 2314 48-49 -16.356805870154535 2314 50-51 -14.300258492979399 2314 52-53 -17.21296467202291 2314 54-55 -18.821211643643796 2314 56-57 -19.024290385199933 2314 58-59 -18.637888894185348 2314 60-61 -18.329362032650113 2314 62-63 -17.843968954659747 2314 64-65 -17.361394710514144 2314 66-67 -16.941530208255646 2314 68-69 -16.48379210505333 2314 70-71 -15.875521717126812 2314 72-73 -15.396626789078358 2314 74-75 -14.868671883921746 2314 76-77 -14.295929950255076 2314 78-79 -13.78565032740351 2314 80-81 -13.279613858898493 2314 82-83 -12.818933166850185 2314 84-85 -12.295766488593756 2314 86-87 -11.747748091211413 2314 88-89 -11.194907108967078 2314 90-91 -10.627152183714422 2314 92-93 -10.006587253118907 2314 94-95 -9.358036801763886 2314 96-97 -8.629942941664382 2314 98-99 -7.843466546793902 2314 100-101 -6.784684875635929 2315 1 -20.866360072363793 2315 2 -19.715115539371997 2315 3 -20.15013757757491 2315 4 -16.660863962152042 2315 5 -22.07312035781921 2315 6 -22.841174463747773 2315 7 -22.944663099035225 2315 8 -23.76847314648629 2315 9 -19.86473770971706 2315 10-11 -18.067660802399054 2315 12-13 -14.450270227442832 2315 14-15 -14.319435876474436 2315 16-17 -20.15026166561443 2315 18-19 -23.888166224166753 2315 20-21 -23.369661587432603 2315 22-23 -23.273727111704066 2315 24-25 -22.978832608319653 2315 26-27 -22.66608357484196 2315 28-29 -22.585146729491385 2315 30-31 -22.49256449506396 2315 32-33 -22.191858365486762 2315 34-35 -19.37851679165244 2315 36-37 -17.677246508415458 2315 38-39 -16.784256960015107 2315 40-41 -18.124775371231223 2315 42-43 -18.049707223125335 2315 44-45 -16.055561939659334 2315 46-47 -16.8603492589865 2315 48-49 -16.356805870154535 2315 50-51 -14.300258492979399 2315 52-53 -17.21296467202291 2315 54-55 -18.821211643643796 2315 56-57 -19.024290385199933 2315 58-59 -18.637888894185348 2315 60-61 -18.329362032650113 2315 62-63 -17.843968954659747 2315 64-65 -17.361394710514144 2315 66-67 -16.941530208255646 2315 68-69 -16.48379210505333 2315 70-71 -15.875521717126812 2315 72-73 -15.396626789078358 2315 74-75 -14.868671883921746 2315 76-77 -14.295929950255076 2315 78-79 -13.78565032740351 2315 80-81 -13.279613858898493 2315 82-83 -12.818933166850185 2315 84-85 -12.295766488593756 2315 86-87 -11.747748091211413 2315 88-89 -11.194907108967078 2315 90-91 -10.627152183714422 2315 92-93 -10.006587253118907 2315 94-95 -9.358036801763886 2315 96-97 -8.629942941664382 2315 98-99 -7.843466546793902 2315 100-101 -6.784684875635929 2316 1 -20.866360072363793 2316 2 -19.715115539371997 2316 3 -20.15013757757491 2316 4 -22.095424564140753 2316 5 -22.07312035781921 2316 6 -22.841174463747773 2316 7 -22.944663099035225 2316 8 -23.76847314648629 2316 9 -24.933805164702278 2316 10-11 -25.2075425546162 2316 12-13 -25.162713119138612 2316 14-15 -25.413362240355113 2316 16-17 -24.523414044007335 2316 18-19 -23.888166224166753 2316 20-21 -23.369661587432603 2316 22-23 -23.273727111704066 2316 24-25 -22.978832608319653 2316 26-27 -22.66608357484196 2316 28-29 -22.585146729491385 2316 30-31 -22.49256449506396 2316 32-33 -22.191858365486762 2316 34-35 -19.37851679165244 2316 36-37 -17.677246508415458 2316 38-39 -16.784256960015107 2316 40-41 -18.124775371231223 2316 42-43 -18.049707223125335 2316 44-45 -16.055561939659334 2316 46-47 -16.8603492589865 2316 48-49 -16.356805870154535 2316 50-51 -14.300258492979399 2316 52-53 -17.21296467202291 2316 54-55 -18.821211643643796 2316 56-57 -19.024290385199933 2316 58-59 -18.637888894185348 2316 60-61 -18.329362032650113 2316 62-63 -17.843968954659747 2316 64-65 -17.361394710514144 2316 66-67 -16.941530208255646 2316 68-69 -16.48379210505333 2316 70-71 -15.875521717126812 2316 72-73 -15.396626789078358 2316 74-75 -14.868671883921746 2316 76-77 -14.295929950255076 2316 78-79 -13.78565032740351 2316 80-81 -13.279613858898493 2316 82-83 -12.818933166850185 2316 84-85 -12.295766488593756 2316 86-87 -11.747748091211413 2316 88-89 -11.194907108967078 2316 90-91 -10.627152183714422 2316 92-93 -10.006587253118907 2316 94-95 -9.358036801763886 2316 96-97 -8.629942941664382 2316 98-99 -7.843466546793902 2316 100-101 -6.784684875635929 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 565065.0 3 33686.0 4 74342.0 5 42692.0 6 37156.0 7 45751.0 8 58438.0 9 70223.0 10 73932.0 11 70414.0 12 63396.0 13 57920.0 14 55454.0 15 59280.0 16 64071.0 17 68193.0 18 70441.0 19 73624.0 20 77348.0 21 82128.0 22 86725.0 23 91758.0 24 97469.0 25 103043.0 26 109942.0 27 116711.0 28 123874.0 29 130886.0 30 136649.0 31 137176.0 32 132961.0 33 130388.0 34 122054.0 35 104605.0 36 73661.0 37 32625.0 38 4358.0 39 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80465992600331 20.301111095025036 14.043074838736178 23.85115414023547 2 15.254832111386355 20.942135071518038 40.56525020667619 23.237782610419416 3 18.266338814481482 26.0308228407403 30.566123008635714 25.1367153361425 4 12.597474458623974 17.288445562676312 38.93303723161753 31.181042747082184 5 13.142237281289711 37.80971002962851 35.60392458075153 13.44412810833025 6 30.291318610521135 37.78760398710935 18.217365804287304 13.703711598082217 7 26.30366338080358 32.91662527837511 23.247495429207035 17.532215911614273 8 24.950022061374185 35.80972122088005 21.216392436306176 18.023864281439586 9 25.99072495137601 16.209233277678294 21.292709901968813 36.507331868976884 10-11 24.456946623988227 26.434613558228904 29.120800043714873 19.987639774068 12-13 24.886675320244002 24.323407369936852 28.997233917410036 21.79268339240911 14-15 22.199522640384686 25.76663161469882 26.63836084252185 25.39548490239465 16-17 21.96205863281228 28.62292705191655 27.33905184821175 22.07596246705942 18-19 21.931003659791205 27.728180392289143 29.090837029666233 21.24997891825342 20-21 22.62707597007698 26.96932732866187 29.02889506621359 21.374701635047565 22-23 22.4013987316838 26.823132152313768 28.755571241152655 22.019897874849782 24-25 22.227919654835592 27.02788351553564 28.72510095990754 22.019095869721234 26-27 22.115370927582973 27.19039268609445 28.61076249306028 22.083473893262294 28-29 22.11934880419863 27.376148818638296 28.59219387039506 21.912308506768014 30-31 22.381400004985284 27.156766946160438 28.43892182020422 22.02291122865006 32-33 22.222773629261443 27.26824115651747 28.31547815261406 22.19350706160703 34-35 22.649257543900696 27.197254750005396 28.27617123646629 21.877316469627623 36-37 23.090957750878328 27.155671098859273 27.858483714004546 21.894887436257854 38-39 22.812183225741233 27.13634666090097 28.01956594380126 22.031904169556537 40-41 22.792490138408215 27.160884385896626 28.06554360621868 21.981081869476483 42-43 22.88213517034165 27.08691281100446 27.76273813220092 22.268213886452973 44-45 23.11174610883497 27.290006033105847 27.50561662388903 22.092631234170156 46-47 22.59279957221845 27.26330981933432 27.758043741885967 22.385846866561263 48-49 23.0265913022756 27.32166026135437 27.277065114239036 22.374683322131 50-51 23.252402932819223 27.431145316725413 27.1413646891302 22.17508706132516 52-53 22.385091344828716 27.469888005880037 27.671624812393535 22.47339583689771 54-55 22.137483840471248 27.402890305010224 27.797018297240754 22.66260755727777 56-57 22.247908508146434 27.568439480841022 27.591017759057372 22.592634251955168 58-59 22.283965161274864 27.434181538883408 27.7058183013792 22.57603499846253 60-61 22.23541895513935 27.504986233220368 27.76257316571151 22.497021645928772 62-63 22.211810156603853 27.460399737424336 27.662881652310574 22.66490845366124 64-65 22.004528069598788 27.339520747240208 27.6714397906416 22.9845113925194 66-67 22.090757669821063 27.4362307347233 27.557753799748557 22.91525779570708 68-69 22.173427074467494 27.34788190157646 27.62156192172313 22.857129102232918 70-71 22.07109503975977 27.19686554201691 27.47604882800026 23.255990590223064 72-73 22.151997882728867 27.29818624331623 27.441316107153117 23.108499766801785 74-75 22.104341977644157 27.469231178725984 27.332087735448514 23.094339108181344 76-77 22.127493346608983 27.28261461009139 27.383984920333916 23.205907122965712 78-79 22.018296670307592 27.368482380352514 27.31490479983641 23.29831614950348 80-81 22.143692916066442 27.378560101997657 27.263460393776068 23.214286588159837 82-83 22.22095149860019 27.260427517051312 27.31013544488034 23.20848553946816 84-85 22.185585566240118 27.34923342124101 27.130684800102046 23.334496212416823 86-87 21.99904679682009 27.367779164968336 27.127857603640788 23.505316434570787 88-89 22.08648276471654 27.199394252410375 27.031665575927438 23.682457406945648 90-91 22.02040245034836 27.167998118140574 27.121839302012756 23.689760129498303 92-93 22.07754134647206 26.96592447836648 27.203698007511655 23.752836167649797 94-95 21.83963375323866 26.979056793477067 27.222255864580003 23.95905358870426 96-97 21.907597490827072 26.771365217239612 27.125328135287035 24.195709156646277 98-99 21.71820631976705 26.837570147021093 27.042066684158232 24.402156849053625 100-101 21.992941195082825 26.889403930853224 26.847512153054957 24.270142721008995 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 42548.0 1 45804.0 2 34035.5 3 20312.0 4 26310.5 5 33119.5 6 34965.0 7 33246.5 8 30403.0 9 27408.5 10 24301.5 11 21212.5 12 17896.0 13 15056.0 14 12762.5 15 10870.5 16 9580.0 17 8765.0 18 8602.5 19 8828.5 20 9348.0 21 10264.0 22 11609.0 23 13643.5 24 16344.5 25 19810.0 26 24172.0 27 29669.0 28 36373.5 29 44172.5 30 52929.0 31 61692.5 32 69717.5 33 76283.5 34 81912.5 35 86777.0 36 91361.5 37 95605.0 38 98047.0 39 99069.5 40 99950.0 41 100733.5 42 101602.5 43 102981.5 44 104352.5 45 105567.5 46 106452.0 47 106778.0 48 105958.0 49 104030.5 50 101019.0 51 97245.0 52 93681.5 53 90116.5 54 85405.5 55 78807.0 56 70432.0 57 62009.5 58 54231.5 59 46513.0 60 39121.5 61 32394.0 62 26349.5 63 20957.5 64 16343.0 65 12578.5 66 9614.5 67 7257.5 68 5475.0 69 4196.5 70 3291.5 71 2590.0 72 1955.5 73 1434.0 74 1083.5 75 773.5 76 532.0 77 378.0 78 260.5 79 164.0 80 103.5 81 70.5 82 45.0 83 28.5 84 16.0 85 11.0 86 8.0 87 4.5 88 2.5 89 3.5 90 3.0 91 2.0 92 1.0 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.613859188747243 2 21.687798550627978 3 17.434264240160623 4 22.69117014597214 5 22.561141788693426 6 16.377823366121678 7 15.201071044987568 8 12.235976026255207 9 3.0964170474852177 10-11 6.616396509731858 12-13 3.6141156231816725 14-15 7.52685374497768 16-17 11.663296412907737 18-19 14.771485870807641 20-21 15.191598494971355 22-23 13.126208864121946 24-25 15.678637056730658 26-27 13.161382354091106 28-29 13.19577964117578 30-31 14.077316131910795 32-33 12.527080856192029 34-35 19.390741107014804 36-37 24.162908311465493 38-39 23.21434526424246 40-41 18.34962305288069 42-43 18.288762997086078 44-45 23.639531955410305 46-47 12.98539991122539 48-49 21.70727549337755 50-51 21.42312774114611 52-53 11.448158846758002 54-55 13.061122814845138 56-57 12.58998203809029 58-59 13.613448663666116 60-61 13.019840320692532 62-63 12.980843312810913 64-65 14.067656718237204 66-67 12.364035128067727 68-69 13.412800218026765 70-71 11.999536003164609 72-73 11.362977767249529 74-75 11.434790908191873 76-77 11.503743597763616 78-79 11.921628232166308 80-81 11.881754402507767 82-83 10.385680839173062 84-85 10.301721560501278 86-87 10.425899647891374 88-89 11.858899539797935 90-91 9.318603110791175 92-93 11.351694077169515 94-95 11.461656152471544 96-97 11.00914424494433 98-99 10.864842379067591 100-101 10.958992914130112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3478472.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.7421154984667 #Duplication Level Percentage of deduplicated Percentage of total 1 98.75963039271083 95.54215570037515 2 0.8618579097678367 1.6675591490005428 3 0.17921872962339727 0.5201399712214553 4 0.0673843204030087 0.26075606828854214 5 0.038195609500364006 0.18475620329092737 6 0.022198623169442235 0.12885250599390718 7 0.01323920125542991 0.08965518358721722 8 0.010192547556885638 0.07888388903774761 9 0.007146822115966113 0.06222588215308109 >10 0.036353751952836244 0.627886565606539 >50 0.0025927289900133615 0.18156679920570803 >100 0.0018705259853765684 0.356249670043947 >500 2.970924215230544E-5 0.02405650941155998 >1k 5.941848430343708E-5 0.09269085167353444 >5k 2.970924215171854E-5 0.18256505111016766 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6352 0.18260891563882073 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8748254980922657E-5 2 0.0 0.0 0.0 0.0 2.8748254980922657E-5 3 0.0 0.0 0.0 0.0 2.8748254980922657E-5 4 0.0 0.0 0.0 0.0 2.8748254980922657E-5 5 0.0 0.0 0.0 0.0 2.8748254980922657E-5 6 0.0 0.0 0.0 0.0 2.8748254980922657E-5 7 0.0 0.0 0.0 0.0 2.8748254980922657E-5 8 0.0 0.0 0.0 0.0 2.8748254980922657E-5 9 0.0 0.0 0.0 2.8748254980922657E-5 2.8748254980922657E-5 10-11 0.0 0.0 0.0 2.8748254980922657E-5 5.7496509961845314E-5 12-13 0.0 0.0 0.0 2.8748254980922657E-5 1.437412749046133E-4 14-15 0.0 0.0 0.0 2.8748254980922657E-5 2.1561191235691993E-4 16-17 0.0 0.0 0.0 2.8748254980922657E-5 2.2998603984738126E-4 18-19 0.0 0.0 0.0 2.8748254980922657E-5 2.587342948283039E-4 20-21 0.0 0.0 0.0 2.8748254980922657E-5 3.1623080479014926E-4 22-23 0.0 0.0 0.0 5.7496509961845314E-5 3.7372731475199456E-4 24-25 0.0 0.0 0.0 1.7248952988553596E-4 3.7372731475199456E-4 26-27 0.0 0.0 0.0 1.8686365737599728E-4 4.3122382471383986E-4 28-29 0.0 2.8748254980922657E-5 0.0 7.618287569944504E-4 4.599720796947625E-4 30-31 0.0 2.8748254980922657E-5 0.0 0.002831703115620882 4.7434620718522384E-4 32-33 0.0 2.8748254980922657E-5 0.0 0.008940707299066947 4.887203346756852E-4 34-35 0.0 2.8748254980922657E-5 0.0 0.018715113992580648 4.887203346756852E-4 36-37 0.0 2.8748254980922657E-5 0.0 0.033779199602584124 4.887203346756852E-4 38-39 0.0 2.8748254980922657E-5 0.0 0.05492354114105274 4.887203346756852E-4 40-41 0.0 2.8748254980922657E-5 0.0 0.07795089338077178 4.887203346756852E-4 42-43 0.0 2.8748254980922657E-5 0.0 0.10368058158869756 4.887203346756852E-4 44-45 1.4374127490461329E-5 2.8748254980922657E-5 0.0 0.13803474629090012 4.887203346756852E-4 46-47 2.8748254980922657E-5 2.8748254980922657E-5 0.0 0.1751918658537427 4.887203346756852E-4 48-49 2.8748254980922657E-5 2.8748254980922657E-5 0.0 0.2155400417194676 5.462168446375305E-4 50-51 2.8748254980922657E-5 2.8748254980922657E-5 0.0 0.26357837579258936 5.749650996184531E-4 52-53 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 0.32896628174669795 5.749650996184531E-4 54-55 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 0.4029930383225738 5.893392271089145E-4 56-57 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 0.489525285815151 6.037133545993758E-4 58-59 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 0.5827989991007546 6.037133545993758E-4 60-61 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 0.6820379752948996 6.037133545993758E-4 62-63 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 0.7828006090030335 6.612098645612212E-4 64-65 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 0.8902615861217225 6.612098645612212E-4 66-67 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 1.0128297712328862 6.612098645612212E-4 68-69 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 1.1470409996113236 6.612098645612212E-4 70-71 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 1.2888705155597056 7.187063745230665E-4 72-73 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 1.4356303572373157 8.193252669562957E-4 74-75 2.8748254980922657E-5 2.8748254980922657E-5 2.8748254980922657E-5 1.5918771230586304 8.76821776918141E-4 76-77 5.7496509961845314E-5 2.8748254980922657E-5 2.8748254980922657E-5 1.768089551964196 9.055700318990638E-4 78-79 5.7496509961845314E-5 2.8748254980922657E-5 2.8748254980922657E-5 1.9630745913723036 9.19944159389525E-4 80-81 8.624476494276798E-5 2.8748254980922657E-5 2.8748254980922657E-5 2.1699757824700043 9.19944159389525E-4 82-83 8.624476494276798E-5 2.8748254980922657E-5 2.8748254980922657E-5 2.3865651354962756 9.486924143704477E-4 84-85 8.624476494276798E-5 2.8748254980922657E-5 2.8748254980922657E-5 2.607581719789609 9.486924143704477E-4 86-87 8.624476494276798E-5 2.8748254980922657E-5 2.8748254980922657E-5 2.846767201230885 0.0010061889243322929 88-89 8.624476494276798E-5 2.8748254980922657E-5 2.8748254980922657E-5 3.10242543277623 0.0010349371793132156 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5090 0.0 47.45393 1 GGTATCA 2655 0.0 46.722385 1 ATCAACG 6210 0.0 38.549698 3 TCAACGC 6325 0.0 38.30908 4 CAACGCA 6510 0.0 36.173843 5 TATCAAC 6610 0.0 35.69076 2 AACGCAG 7115 0.0 33.708817 6 ACGCAGA 8070 0.0 29.119106 7 CGCAGAG 8535 0.0 27.833826 8 GTACATG 6425 0.0 27.076992 1 TACATGG 6875 0.0 24.929733 2 ACATGGG 7205 0.0 23.852797 3 GCAGAGT 9320 0.0 23.17413 9 GTGGTAT 1330 0.0 21.61114 1 GAGTACA 5195 0.0 19.898613 1 CATGGGG 5520 0.0 19.712051 4 AGAGTAC 8720 0.0 19.258232 10-11 TATACCG 385 9.094947E-12 18.83984 5 AGTACAT 5360 0.0 18.245188 2 CAGAGTA 8915 0.0 18.24214 10-11 >>END_MODULE