FastQCFastQC Report
Sat 18 Jun 2016
SRR1930920_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930920_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3478472
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51100.1469035829525148No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45620.13114953922296915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.056.1811
GTATCAA69750.040.97951
ATCAACG88300.031.8259753
CAACGCA88800.031.6902085
TCAACGC89200.031.5576254
TATCAAC95050.029.7165282
AACGCAG95200.029.6586176
ACGCAGA108600.025.6058187
CGCAGAG111000.024.8811728
GTACATG89700.023.7666681
TACATGG92550.022.7227292
ACATGGG91600.022.5949693
GTGGTAT17000.021.2265341
GCAGAGT129600.020.9441099
CATGGGG68200.019.6977464
AGAGTAC120750.019.25660910-11
CAGAGTA124600.018.41405110-11
GAGTACT83700.017.46208812-13
AGTACTT91200.017.3008612-13
GAGTACA69750.017.0861381
Produced by FastQC (version 0.11.5)