##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930920_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3478472 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.231914185308952 31.0 31.0 33.0 30.0 34.0 2 31.35468907037343 31.0 31.0 34.0 30.0 34.0 3 31.437574889204225 31.0 31.0 34.0 28.0 34.0 4 35.30962129348749 37.0 35.0 37.0 33.0 37.0 5 35.08863719472228 35.0 35.0 37.0 32.0 37.0 6 35.12105372703877 37.0 35.0 37.0 32.0 37.0 7 35.000462559422644 36.0 35.0 37.0 32.0 37.0 8 34.974145544365456 36.0 35.0 37.0 32.0 37.0 9 36.476768247667366 38.0 35.0 39.0 32.0 39.0 10-11 36.40076202999478 38.0 35.0 39.0 32.0 39.0 12-13 36.30401437757728 38.0 35.0 39.0 32.0 39.0 14-15 37.318479924518584 39.0 36.0 41.0 32.0 41.0 16-17 37.28216354767265 39.0 36.0 40.5 32.0 41.0 18-19 37.26965072595094 39.0 36.0 40.0 31.5 41.0 20-21 37.23312851159935 39.0 36.0 40.0 31.0 41.0 22-23 37.10722466646274 39.0 36.0 40.0 31.0 41.0 24-25 36.98760030266163 39.0 36.0 40.0 31.0 41.0 26-27 36.71176668951195 39.0 35.5 40.0 30.0 41.0 28-29 36.52137087778772 38.0 35.0 40.0 30.0 41.0 30-31 36.280307704072364 38.0 35.0 40.0 30.0 41.0 32-33 36.04386940587706 38.0 34.5 40.0 29.5 41.0 34-35 36.231748451618984 38.0 35.0 40.0 30.0 41.0 36-37 36.2794271450223 38.0 35.0 40.0 30.0 41.0 38-39 36.235936641145884 38.0 35.0 40.0 30.0 41.0 40-41 36.138028134192254 38.0 35.0 40.0 30.0 41.0 42-43 35.99355866598897 38.0 35.0 40.0 29.0 41.0 44-45 35.81285360353627 38.0 34.5 40.0 28.5 41.0 46-47 35.59420458178189 38.0 34.0 40.0 28.0 41.0 48-49 35.39834444549216 38.0 34.0 40.0 27.0 41.0 50-51 35.12282921926639 38.0 34.0 40.0 26.5 41.0 52-53 34.912164019144036 38.0 33.0 40.0 26.0 41.0 54-55 34.734029769393 38.0 33.0 40.0 26.0 41.0 56-57 34.47586972095794 37.5 33.0 40.0 26.0 41.0 58-59 34.233136992334565 37.0 33.0 40.0 25.5 41.0 60-61 33.92332035445449 37.0 32.5 40.0 24.5 41.0 62-63 33.61711205379834 36.0 32.0 39.0 24.0 41.0 64-65 33.30710768981323 36.0 32.0 39.0 23.0 40.5 66-67 32.97262806772629 35.5 31.0 39.0 23.0 40.0 68-69 32.59987172528628 35.0 31.0 38.5 22.0 40.0 70-71 32.20388808649315 35.0 31.0 38.0 22.0 40.0 72-73 31.786825939665462 35.0 30.5 37.0 20.5 39.5 74-75 31.262265011763787 34.0 30.0 37.0 19.5 39.0 76-77 29.882724656113375 32.5 28.5 35.0 18.0 38.0 78-79 30.65083591301008 34.0 29.5 36.0 19.0 38.0 80-81 30.657522469636092 34.0 30.0 35.5 19.0 37.5 82-83 30.38301084499171 34.0 30.0 35.0 18.0 37.0 84-85 30.064810353511543 34.0 29.5 35.0 17.5 36.5 86-87 29.728110359951152 34.0 29.0 35.0 14.0 36.0 88-89 29.379294126846503 34.0 29.0 35.0 9.0 36.0 90-91 29.0424544167669 34.0 29.0 35.0 6.5 35.5 92-93 28.690276506466056 33.5 29.0 35.0 2.0 35.0 94-95 28.26959524181882 33.0 29.0 35.0 2.0 35.0 96-97 27.80794210791405 33.0 27.0 35.0 2.0 35.0 98-99 27.321432945270224 33.0 27.0 35.0 2.0 35.0 100-101 26.111726930675307 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5381338099457871 1101 2 0.6112232315391601 1101 3 0.5783141661297222 1101 4 0.350879078614625 1101 5 0.45015561893053757 1101 6 0.4841599378332262 1101 7 0.5577981233934537 1101 8 0.5092385189280151 1101 9 0.6707740642907822 1101 10-11 0.5693081235341211 1101 12-13 0.5595463909614651 1101 14-15 0.718058544028942 1101 16-17 0.697889545344097 1101 18-19 0.6694995480114159 1101 20-21 0.6069407150096993 1101 22-23 0.7290562861380536 1101 24-25 0.6708479122537341 1101 26-27 0.6219602149316472 1101 28-29 0.6910912591986857 1101 30-31 0.6862442332470593 1101 32-33 0.5732727931783046 1101 34-35 0.510715721026159 1101 36-37 0.7378738390099002 1101 38-39 0.8594612118390259 1101 40-41 0.837095537830443 1101 42-43 0.7203330563222181 1101 44-45 0.8511351619764795 1101 46-47 0.8922522370924497 1101 48-49 0.8879857954938544 1101 50-51 0.7949332806235603 1101 52-53 0.6526674586195611 1101 54-55 0.6509198327447976 1101 56-57 0.7416696041855246 1101 58-59 0.6510522675074029 1101 60-61 0.6509153685028508 1101 62-63 0.646681680746596 1101 64-65 0.7560420249803386 1101 66-67 0.8218292189801986 1101 68-69 0.677904497963496 1101 70-71 0.49328165344959984 1101 72-73 0.5931410928333811 1101 74-75 0.7549567547917455 1101 76-77 0.8452785009785124 1101 78-79 0.7796759862479092 1101 80-81 0.6823514190674942 1101 82-83 0.8167560431983354 1101 84-85 0.8904141865238238 1101 86-87 0.8936748234321747 1101 88-89 0.7471637069700172 1101 90-91 0.7354463778129805 1101 92-93 0.8321821226277883 1101 94-95 0.8916418213093245 1101 96-97 0.7461707273098739 1101 98-99 0.7797995880469628 1101 100-101 1.0007361000098172 1102 1 0.47967778540317596 1102 2 0.5187958629870053 1102 3 0.46677450070635373 1102 4 0.2709917786297069 1102 5 0.38993469281403037 1102 6 0.37909179668698556 1102 7 0.4078273759057254 1102 8 0.4206544552378375 1102 9 0.7024709230462136 1102 10-11 0.6336846271296395 1102 12-13 0.6336720112458281 1102 14-15 0.8304772187672214 1102 16-17 0.7269433126088103 1102 18-19 0.7487156380403235 1102 20-21 0.7430911253642662 1102 22-23 0.8061981927791066 1102 24-25 0.7198527122878176 1102 26-27 0.8112618665104634 1102 28-29 0.915675250781824 1102 30-31 0.9208989552744455 1102 32-33 0.7955914767660843 1102 34-35 0.6585639816078626 1102 36-37 0.8136270297461081 1102 38-39 0.9482667588364322 1102 40-41 0.9036273452260062 1102 42-43 0.8295318652451371 1102 44-45 0.8587811705244377 1102 46-47 0.8458685686799186 1102 48-49 0.8726061028009156 1102 50-51 0.8806742787160005 1102 52-53 0.7496965259478685 1102 54-55 0.7557885323927849 1102 56-57 0.6189574046588078 1102 58-59 0.7353862361473986 1102 60-61 0.7974602125222248 1102 62-63 0.5167566474790348 1102 64-65 0.5344595705880408 1102 66-67 0.6411244464778889 1102 68-69 0.6361667348547613 1102 70-71 0.6178735931943464 1102 72-73 0.5811334281835236 1102 74-75 0.7024885638261047 1102 76-77 0.9125129422473321 1102 78-79 0.9325569080379985 1102 80-81 0.8306540768671802 1102 82-83 0.9136039825759816 1102 84-85 0.9943452079527191 1102 86-87 0.8690306880282783 1102 88-89 0.7786417044921627 1102 90-91 0.7620609608048987 1102 92-93 0.9401761705199689 1102 94-95 0.9619225389054087 1102 96-97 0.9136316911842535 1102 98-99 0.8938947922737412 1102 100-101 1.2061925129471334 1103 1 0.42354381512640416 1103 2 0.49647101944131933 1103 3 0.45071763998569025 1103 4 0.3050626694931253 1103 5 0.3385494046441764 1103 6 0.3616683708708379 1103 7 0.4234726081416369 1103 8 0.4167209745923941 1103 9 0.6025542297604787 1103 10-11 0.5105584998071535 1103 12-13 0.5748181505239671 1103 14-15 0.6523522698618791 1103 16-17 0.7098582978875712 1103 18-19 0.636478723156344 1103 20-21 0.6734745476072774 1103 22-23 0.600351369460725 1103 24-25 0.6821167646629789 1103 26-27 0.7744533790357764 1103 28-29 0.7724292957833967 1103 30-31 0.7922748240829307 1103 32-33 1.017077869537502 1103 34-35 0.8315522680218521 1103 36-37 0.9515273177105783 1103 38-39 0.9483893860007981 1103 40-41 0.9714416027574089 1103 42-43 1.0923720459596922 1103 44-45 1.1135320260233854 1103 46-47 1.043704069622855 1103 48-49 0.9964988193854154 1103 50-51 0.9423346066549314 1103 52-53 1.0218326831754325 1103 54-55 1.1278223208623217 1103 56-57 1.156358051731445 1103 58-59 1.127282833732103 1103 60-61 1.0835796948481686 1103 62-63 1.0725283192880681 1103 64-65 0.9480774069184648 1103 66-67 0.8650885460296109 1103 68-69 0.9417782336538139 1103 70-71 0.7915445639747816 1103 72-73 0.8205052421204293 1103 74-75 1.1162307022285098 1103 76-77 1.129617896344893 1103 78-79 1.231053678457215 1103 80-81 1.1462417640630314 1103 82-83 0.9930259081534594 1103 84-85 1.152882590893629 1103 86-87 1.2325838609612916 1103 88-89 1.2890123735100794 1103 90-91 1.2573707010705952 1103 92-93 1.2803791772981619 1103 94-95 1.2881445734316337 1103 96-97 1.2463004849835855 1103 98-99 1.3106002809061046 1103 100-101 1.4221151019076572 1104 1 0.3792949930987177 1104 2 0.4298542285741327 1104 3 0.4223890241299344 1104 4 0.2181204425270309 1104 5 0.3430596485093602 1104 6 0.34976919729095357 1104 7 0.41262566956571334 1104 8 0.37348806345700325 1104 9 0.5275887143818778 1104 10-11 0.4000961776905143 1104 12-13 0.44155930490423856 1104 14-15 0.5484123378345345 1104 16-17 0.4880100665458258 1104 18-19 0.6162998641618884 1104 20-21 0.5508139601066873 1104 22-23 0.46906718171909034 1104 24-25 0.41909189289174265 1104 26-27 0.6350293304247998 1104 28-29 0.5330539820222455 1104 30-31 0.5597639524625819 1104 32-33 0.743071939393424 1104 34-35 0.6545909755540791 1104 36-37 0.5412755823028661 1104 38-39 0.44396448051334403 1104 40-41 0.485678442485181 1104 42-43 0.5183785498384523 1104 44-45 0.5841253291611324 1104 46-47 0.576016928265247 1104 48-49 0.6101596425270444 1104 50-51 0.5341046806164158 1104 52-53 0.5252594905561168 1104 54-55 0.5977726173695288 1104 56-57 0.5702952873920353 1104 58-59 0.5526158280403948 1104 60-61 0.37055286716322655 1104 62-63 0.3722726767098763 1104 64-65 0.41137550120654254 1104 66-67 0.43246290418721856 1104 68-69 0.4857694106202288 1104 70-71 0.4017855651418074 1104 72-73 0.3588157704313524 1104 74-75 0.4963028055901617 1104 76-77 0.6105445942875498 1104 78-79 0.7214315144156629 1104 80-81 0.6178006831692322 1104 82-83 0.7856991718376385 1104 84-85 0.7960546591419764 1104 86-87 0.6897912632564207 1104 88-89 0.6984908244636792 1104 90-91 0.7061611547214923 1104 92-93 0.735372205862884 1104 94-95 0.6999432270178936 1104 96-97 0.5890622537629184 1104 98-99 0.6555609390990114 1104 100-101 1.049479312598681 1105 1 0.2259943850488817 1105 2 0.3118982609403851 1105 3 0.31893321963742594 1105 4 0.1894912777252742 1105 5 0.20236670458022132 1105 6 0.2697657040064243 1105 7 0.3141247495461599 1105 8 0.2991393244963234 1105 9 0.4962446731611152 1105 10-11 0.36639614045306956 1105 12-13 0.3245861267794723 1105 14-15 0.4631156997570045 1105 16-17 0.3967918260210581 1105 18-19 0.41539552193644624 1105 20-21 0.3869998392317697 1105 22-23 0.5586124664521037 1105 24-25 0.519655427610509 1105 26-27 0.6004788664123026 1105 28-29 0.5783164271515986 1105 30-31 0.6939847680717435 1105 32-33 0.654474117116699 1105 34-35 0.6767928910384242 1105 36-37 0.6925946914182717 1105 38-39 0.5095367969612496 1105 40-41 0.5754241665472435 1105 42-43 0.48167177104706127 1105 44-45 0.5812456437022959 1105 46-47 0.5308386312657092 1105 48-49 0.4478718625942619 1105 50-51 0.5428594217191502 1105 52-53 0.4952086014060342 1105 54-55 0.5852528918349549 1105 56-57 0.6747772442135869 1105 58-59 0.5319209979802011 1105 60-61 0.5927466862329283 1105 62-63 0.5308160922930796 1105 64-65 0.5748528779439326 1105 66-67 0.4889188700015481 1105 68-69 0.5605939276554537 1105 70-71 0.5259895693191936 1105 72-73 0.5539669580874396 1105 74-75 0.727248939870563 1105 76-77 0.7329044656667563 1105 78-79 0.7924708817915409 1105 80-81 0.7049646516374892 1105 82-83 0.7578217303289243 1105 84-85 0.8093063975968526 1105 86-87 0.8734766238470719 1105 88-89 0.765695311393582 1105 90-91 0.55871331443252 1105 92-93 0.6408937180669376 1105 94-95 0.5310277584386576 1105 96-97 0.4338063767362357 1105 98-99 0.6344095864822563 1105 100-101 0.9912909566378403 1106 1 0.1591578562366287 1106 2 0.22384710112094908 1106 3 0.22886118459754812 1106 4 0.10814450600799574 1106 5 0.047122546618425076 1106 6 0.155922870510544 1106 7 0.18436814868709916 1106 8 0.09371887303909432 1106 9 0.13727946950889702 1106 10-11 0.1910727886531376 1106 12-13 0.11483510237132322 1106 14-15 0.25619716535182846 1106 16-17 0.14645226839596148 1106 18-19 0.2994987251016141 1106 20-21 0.21483177041318413 1106 22-23 0.35844802585601343 1106 24-25 0.2010259639497889 1106 26-27 0.33766111753377004 1106 28-29 0.3956038367254493 1106 30-31 0.2604858568914281 1106 32-33 0.37691568496999395 1106 34-35 0.33990996238993887 1106 36-37 0.3434315119942042 1106 38-39 0.29791622032799836 1106 40-41 0.344702039684158 1106 42-43 0.3650893883966404 1106 44-45 0.27047159349305616 1106 46-47 0.12615785366845245 1106 48-49 0.32050069449250174 1106 50-51 0.29495926724911214 1106 52-53 0.10689526604134159 1106 54-55 0.2692759188124967 1106 56-57 0.3946214682927902 1106 58-59 0.3338411088503932 1106 60-61 0.16538775338209888 1106 62-63 0.1448831897033216 1106 64-65 0.11923110366784329 1106 66-67 0.017451122240657924 1106 68-69 0.22028218757923668 1106 70-71 0.025808246429747328 1106 72-73 0.08315464918970505 1106 74-75 0.25507369460672535 1106 76-77 0.30238216162782905 1106 78-79 0.32551855927032847 1106 80-81 0.2441443662973164 1106 82-83 0.44124064179159106 1106 84-85 0.40558929306898506 1106 86-87 0.4642523328739827 1106 88-89 0.4183635960937515 1106 90-91 0.45319589272930116 1106 92-93 0.3845668286324333 1106 94-95 0.5369297964314619 1106 96-97 0.4460750099057691 1106 98-99 0.4603178479825125 1106 100-101 0.6762486861987931 1107 1 0.06429996046366426 1107 2 0.1476126472784891 1107 3 0.11331566024463768 1107 4 -0.07921640891136406 1107 5 0.06989997710322626 1107 6 0.16125947799157814 1107 7 0.16820549814710972 1107 8 0.09627349109549499 1107 9 0.10491847975845303 1107 10-11 0.07246552174908771 1107 12-13 0.12000031789060017 1107 14-15 0.08418137138828996 1107 16-17 0.1777509445765446 1107 18-19 0.17294295810027904 1107 20-21 0.253855892500674 1107 22-23 0.21626925554944165 1107 24-25 0.20721463843803178 1107 26-27 0.30235653200011825 1107 28-29 0.35041607978141087 1107 30-31 0.2603328848886193 1107 32-33 0.3511432842852287 1107 34-35 0.3556210361238854 1107 36-37 0.2865467933072665 1107 38-39 0.2957625241905433 1107 40-41 0.10846894431366394 1107 42-43 0.3081268166019697 1107 44-45 0.2803874315493857 1107 46-47 0.3469243714788277 1107 48-49 0.46666421998054375 1107 50-51 0.3226676344003394 1107 52-53 0.23955811286901962 1107 54-55 0.3733176031796006 1107 56-57 0.2679652549528342 1107 58-59 0.37865212007159954 1107 60-61 0.28945840889088004 1107 62-63 0.25450784850236374 1107 64-65 0.31318636389006116 1107 66-67 0.3092130546324938 1107 68-69 0.3791985126132289 1107 70-71 0.348671703879063 1107 72-73 0.3964836192952639 1107 74-75 0.4821671805033674 1107 76-77 0.3704788790760212 1107 78-79 0.520683204922122 1107 80-81 0.4835478531955495 1107 82-83 0.6863086977836623 1107 84-85 0.6535535997935362 1107 86-87 0.6719608266624277 1107 88-89 0.6026420460081567 1107 90-91 0.705239788642718 1107 92-93 0.7433823043911865 1107 94-95 0.8223607313710168 1107 96-97 0.7813228677221034 1107 98-99 0.5480490904202568 1107 100-101 0.7504408270930583 1108 1 0.3215743785998413 1108 2 0.382799841536972 1108 3 0.29627943632113585 1108 4 0.1316735018070645 1108 5 0.2238291743884986 1108 6 0.22568652269154654 1108 7 0.3045991843900566 1108 8 0.33595257649071897 1108 9 0.30567732618555254 1108 10-11 0.3414703276863591 1108 12-13 0.33030474418415423 1108 14-15 0.4367152648974084 1108 16-17 0.47757406155876225 1108 18-19 0.4351118368182867 1108 20-21 0.3748873523122995 1108 22-23 0.4397036555442142 1108 24-25 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-0.6433788897892789 2316 76-77 -0.9757454031742441 2316 78-79 -0.9580352578742009 2316 80-81 -1.046256027875419 2316 82-83 -1.2567060099828886 2316 84-85 -1.1176988529529766 2316 86-87 -1.137683194956935 2316 88-89 -1.2758447148836076 2316 90-91 -0.9190957105217308 2316 92-93 -1.142880640968297 2316 94-95 -0.9697040207952448 2316 96-97 -0.8852573997421516 2316 98-99 -0.8949152213705887 2316 100-101 -1.0230873029155774 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 19.0 4 77.0 5 113.0 6 246.0 7 689.0 8 1707.0 9 2918.0 10 4710.0 11 7145.0 12 9706.0 13 12344.0 14 14597.0 15 17557.0 16 20341.0 17 22773.0 18 21021.0 19 19299.0 20 19454.0 21 20979.0 22 23506.0 23 27146.0 24 33633.0 25 42163.0 26 53700.0 27 68332.0 28 86244.0 29 107338.0 30 130478.0 31 156588.0 32 187267.0 33 225205.0 34 276563.0 35 342014.0 36 438891.0 37 551027.0 38 454984.0 39 77684.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.9644827629834 21.492730353090565 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27.925968643703325 29.215342253725197 21.1876076622149 20-21 22.34787285911745 27.17366993323505 29.046575622859695 21.431881584787803 22-23 22.14501870578963 27.021087009987298 28.880770373513514 21.953123910709554 24-25 22.05957183302017 27.243984112491287 28.879724222471122 21.816719832017423 26-27 21.97270045404372 27.404795208844735 28.720698353658474 21.90180598345307 28-29 22.005246271313787 27.557744129208512 28.662367554935425 21.77464204454228 30-31 22.308948465463953 27.260547689211528 28.600517501617194 21.829986343707326 32-33 22.119603520365576 27.401603987915024 28.444029356124595 22.034763135594805 34-35 22.345977270097116 27.337197124511302 28.54777182691001 21.769053778481563 36-37 22.364175553403577 27.185875669101613 28.507138880619724 21.94280989687509 38-39 22.20795500983987 27.227519042607923 28.592710631561562 21.971815315990646 40-41 22.268234803019148 27.219165497665376 28.595733695109228 21.91686600420625 42-43 22.251304986838004 27.241650556782883 28.322095707084937 22.184948749294175 44-45 22.20984355294706 27.370838209530746 28.33193725902624 22.08738097849595 46-47 22.392859309408557 27.368828249727517 28.037692983770086 22.200619457093843 48-49 22.302021965118822 27.42803575807951 27.97148542429584 22.29845685250583 50-51 22.31633828782118 27.481406268003227 27.902912201866574 22.29934324230902 52-53 22.475213451012724 27.459804595375342 27.842189272721285 22.222792680890645 54-55 22.245884700743606 27.411137153734977 27.92736192565196 22.415616219869456 56-57 22.283834450006047 27.562368184725322 27.774178287063783 22.379619078204847 58-59 22.215867585472377 27.52362825029965 27.89961571700071 22.36088844722726 60-61 22.301179060013812 27.598075466629158 27.906999487715378 22.193745985641648 62-63 22.262528440127785 27.57264723364048 27.83331872928365 22.331505596948084 64-65 22.02297025326446 27.44611297264327 27.864903750066013 22.66601302402626 66-67 22.058114634335162 27.55136839942292 27.787183722123558 22.60333324411836 68-69 22.14511193526228 27.487703683153754 27.847333718772855 22.51985066281112 70-71 22.119459358823807 27.299352240024838 27.81360937636233 22.76757902478903 72-73 22.171739961280522 27.42816982081706 27.683475552631403 22.716614665271013 74-75 22.148319013425787 27.639018723069565 27.524844702148183 22.687817561356468 76-77 22.13661984928952 27.48498914691352 27.595617818526012 22.782773185270948 78-79 22.049747164413354 27.55655612300271 27.589460615019313 22.804236097564623 80-81 22.15076780793927 27.59367952573013 27.49264289768852 22.76290976864208 82-83 22.242539252867353 27.46905825316403 27.61923913718437 22.669163356784246 84-85 22.261929950851982 27.512827471372486 27.383374079193395 22.841868498582134 86-87 22.073585758344468 27.58987279472136 27.43487657799172 22.901664868942458 88-89 22.12600654885625 27.437680934433818 27.373428547608576 23.062883969101357 90-91 22.0466976197804 27.423204312831817 27.455418589376933 23.074679478010847 92-93 22.11519813449439 27.308782439864544 27.502866331548482 23.07315309409259 94-95 21.88904732608483 27.273043508259498 27.558949178075103 23.27895998758057 96-97 21.94462108650005 27.14516603842147 27.529084609564197 23.381128265514285 98-99 21.765591328606355 27.23220138037621 27.432605465848226 23.56960182516921 100-101 21.819551803205545 27.153790514915745 27.39862502846077 23.62803265341794 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 261.0 1 196.5 2 183.0 3 231.0 4 202.0 5 217.5 6 336.0 7 654.0 8 1061.0 9 1270.0 10 1197.0 11 1050.0 12 1089.0 13 1242.5 14 1452.0 15 1669.0 16 1926.0 17 2220.0 18 2541.0 19 2965.0 20 3450.0 21 4082.5 22 4976.5 23 6296.0 24 8414.5 25 11714.0 26 16230.5 27 22041.0 28 29231.0 29 39240.5 30 50892.5 31 61732.0 32 73630.0 33 82386.0 34 87346.5 35 92424.0 36 99789.5 37 107686.5 38 110605.0 39 110604.5 40 110987.0 41 111528.5 42 113297.0 43 116047.5 44 118229.0 45 122636.5 46 126464.5 47 127593.5 48 128706.0 49 127646.0 50 124269.5 51 120805.5 52 117649.5 53 115881.0 54 113031.5 55 104568.0 56 93301.5 57 81623.5 58 70970.0 59 61475.0 60 51047.0 61 41506.0 62 33758.0 63 26220.0 64 19246.5 65 14134.0 66 10363.0 67 7875.5 68 5943.5 69 4358.5 70 3343.0 71 2531.0 72 1875.5 73 1491.0 74 1140.5 75 822.5 76 595.0 77 375.5 78 207.0 79 121.5 80 81.5 81 50.0 82 33.0 83 12.5 84 6.0 85 5.0 86 4.5 87 3.0 88 1.0 89 0.5 90 1.0 91 1.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020813736606188005 2 0.0037372731475199456 3 0.0036222801275962546 4 0.033779199602584124 5 0.0019836295936836636 6 2.874825498092266E-5 7 0.0 8 0.0 9 2.874825498092266E-5 10-11 0.0 12-13 0.0 14-15 2.874825498092266E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.006188924332293E-4 24-25 4.5997207969476257E-4 26-27 0.001796765936307666 28-29 4.312238247138399E-5 30-31 0.0063821126057648295 32-33 0.021992415060405834 34-35 0.013066081888829349 36-37 5.893392271089145E-4 38-39 6.468357370707598E-4 40-41 0.002170493251059661 42-43 0.007934518374734655 44-45 0.0076614099524158884 46-47 0.007618287569944504 48-49 0.009228189848876174 50-51 0.19755800822890052 52-53 0.04995009302935312 54-55 0.018010781745548047 56-57 0.010248752900698928 58-59 0.02002315959421263 60-61 0.0543054536589629 62-63 0.09798842710247488 64-65 0.10310561648907912 66-67 0.10691476027405136 68-69 0.10495987893534864 70-71 0.09386305251271249 72-73 0.07024636104588451 74-75 0.2520790738002203 76-77 0.3037109397459574 78-79 0.006425234988236214 80-81 8.76821776918141E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 5.749650996184532E-5 90-91 0.005764025123674993 92-93 0.017090837586158522 94-95 0.0014949092590079783 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3478472.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.88221236151074 #Duplication Level Percentage of deduplicated Percentage of total 1 75.95324322151046 42.44435267249923 2 13.361265991665295 14.933142071297425 3 4.36016919464498 7.30967702601804 4 2.0404498857688087 4.560994153182116 5 1.105644941496885 3.0892942708579523 6 0.6793718237093299 2.277888031497097 7 0.4529223106177259 1.7717210521644124 8 0.3317984162975284 1.4833303648601142 9 0.2530040619073905 1.2724584044250244 >10 1.3503532193085341 13.433815773588314 >50 0.07676995822712909 2.9473550284434507 >100 0.03274291796553766 3.159180220121703 >500 0.0016465868263187597 0.6477562433773185 >1k 5.660142163154198E-4 0.5220983721142898 >5k 5.145583784685633E-5 0.14693631555345157 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5110 0.1469035829525148 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4562 0.13114953922296915 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4374127490461328E-4 2 0.0 0.0 0.0 0.0 1.4374127490461328E-4 3 0.0 0.0 0.0 0.0 1.4374127490461328E-4 4 0.0 0.0 0.0 0.0 1.4374127490461328E-4 5 0.0 0.0 0.0 0.0 1.7248952988553596E-4 6 0.0 0.0 0.0 0.0 2.012377848664586E-4 7 0.0 0.0 0.0 0.0 2.012377848664586E-4 8 0.0 0.0 0.0 0.0 2.012377848664586E-4 9 0.0 0.0 0.0 0.0 2.587342948283039E-4 10-11 0.0 0.0 0.0 0.0 2.8748254980922656E-4 12-13 0.0 0.0 0.0 0.0 5.174685896566078E-4 14-15 0.0 0.0 0.0 0.0 6.899581195421438E-4 16-17 0.0 0.0 0.0 0.0 8.049511394658344E-4 18-19 0.0 0.0 0.0 0.0 8.624476494276797E-4 20-21 0.0 0.0 0.0 0.0 9.918147968418318E-4 22-23 0.0 0.0 0.0 4.312238247138399E-5 0.001006188924332293 24-25 0.0 0.0 0.0 3.018566772996879E-4 0.0010349371793132156 26-27 0.0 0.0 0.0 4.455979522043012E-4 0.0010780595617845997 28-29 0.0 0.0 0.0 0.0016817729163839754 0.0011211819442559837 30-31 0.0 0.0 0.0 0.0066120986456122115 0.001135556071746445 32-33 0.0 0.0 0.0 0.020238771506569553 0.0011930525817082903 34-35 0.0 0.0 0.0 0.04270553277416061 0.0012361749641796743 36-37 0.0 0.0 0.0 0.0773903024086438 0.0012505490916701356 38-39 0.0 0.0 0.0 0.1275703814778443 0.0012792973466510583 40-41 0.0 0.0 0.0 0.1820626987941832 0.0012936714741415196 42-43 0.0 0.0 0.0 0.24033541164051342 0.0012936714741415196 44-45 0.0 0.0 0.0 0.31440529059886063 0.0013224197291224421 46-47 0.0 0.0 0.0 0.3972865097088607 0.0014230386215556717 48-49 0.0 0.0 0.0 0.4837181383090046 0.0014805351315175168 50-51 0.0 0.0 0.0 0.5720184034829086 0.0015236575139889008 52-53 0.0 0.0 0.0 0.666815774282501 0.0016099022789316689 54-55 0.0 0.0 0.0 0.7730693246919913 0.0016099022789316689 56-57 0.0 0.0 0.0 0.8978798736916669 0.0016242764064221301 58-59 0.0 0.0 0.0 1.0314155180780527 0.0016386505339125914 60-61 0.0 0.0 0.0 1.1764073420743362 0.0016386505339125914 62-63 0.0 0.0 0.0 1.3219022605327857 0.0017536435538362822 64-65 0.0 0.0 0.0 1.48031951960516 0.0017823918088172048 66-67 0.0 0.0 0.0 1.6629715576264519 0.001796765936307666 68-69 0.0 0.0 0.0 1.8653880209471285 0.0018111400637981275 70-71 0.0 0.0 0.0 2.08199174810089 0.0018111400637981275 72-73 0.0 0.0 0.0 2.309347322617517 0.0019405072112122796 74-75 0.0 0.0 0.0 2.552399444353728 0.0020267519761550477 76-77 0.0 0.0 0.0 2.8293888810949177 0.0020698743586264313 78-79 0.0 0.0 0.0 3.1351984434544824 0.002141744996078738 80-81 0.0 0.0 0.0 3.461735497655292 0.002184867378550122 82-83 0.0 0.0 0.0 3.8101355997690938 0.0023286086534547354 84-85 0.0 0.0 0.0 4.175583417086583 0.0023286086534547354 86-87 0.0 0.0 0.0 4.57491105289909 0.002386105163416581 88-89 0.0 0.0 0.0 5.010447115860067 0.002457975800868887 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3820 0.0 56.181 1 GTATCAA 6975 0.0 40.9795 1 ATCAACG 8830 0.0 31.825975 3 CAACGCA 8880 0.0 31.690208 5 TCAACGC 8920 0.0 31.557625 4 TATCAAC 9505 0.0 29.716528 2 AACGCAG 9520 0.0 29.658617 6 ACGCAGA 10860 0.0 25.605818 7 CGCAGAG 11100 0.0 24.881172 8 GTACATG 8970 0.0 23.766668 1 TACATGG 9255 0.0 22.722729 2 ACATGGG 9160 0.0 22.594969 3 GTGGTAT 1700 0.0 21.226534 1 GCAGAGT 12960 0.0 20.944109 9 CATGGGG 6820 0.0 19.697746 4 AGAGTAC 12075 0.0 19.256609 10-11 CAGAGTA 12460 0.0 18.414051 10-11 GAGTACT 8370 0.0 17.462088 12-13 AGTACTT 9120 0.0 17.30086 12-13 GAGTACA 6975 0.0 17.086138 1 >>END_MODULE