FastQCFastQC Report
Sat 18 Jun 2016
SRR1930919_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930919_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3538751
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN65390.1847827100578707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29750.053.94191
GTATCAA64250.049.0114481
TCAACGC77800.040.1813934
ATCAACG77500.040.158623
TATCAAC79850.038.9210052
CAACGCA82850.036.908975
AACGCAG89800.034.0680166
ACGCAGA103350.029.2213447
CGCAGAG105800.028.2784258
GTACATG67550.027.6415541
TACATGG71000.026.220822
GCAGAGT111250.025.302259
ACATGGG74200.024.9492473
AGAGTAC101800.020.38087310-11
CAGAGTA107150.020.22934310-11
CATGGGG53200.019.8083154
GAGTACT75250.019.74375712-13
GTGGTAT14900.018.966531
AGTACTT81650.018.78586612-13
GTATAGG9600.017.8728491
Produced by FastQC (version 0.11.5)