##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930919_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3538751 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.86552713090014 30.0 10.0 31.0 2.0 33.0 2 21.716617247158673 30.0 10.0 31.0 2.0 33.0 3 22.17761323133501 28.0 10.0 31.0 2.0 33.0 4 24.085127775308294 33.0 2.0 35.0 2.0 37.0 5 24.10716747236525 32.0 10.0 35.0 2.0 37.0 6 24.881293428105 32.0 11.0 35.0 2.0 37.0 7 24.97596411841353 32.0 11.0 35.0 2.0 37.0 8 25.778999709219438 33.0 17.0 35.0 2.0 37.0 9 26.907721961788212 33.0 17.0 37.0 2.0 39.0 10-11 27.175771903702746 33.5 17.5 37.0 2.0 39.0 12-13 27.127965770973994 34.0 17.5 37.0 2.0 39.0 14-15 27.3678632658811 34.0 17.0 38.0 2.0 40.0 16-17 26.51969254123842 33.0 13.5 38.0 2.0 40.0 18-19 25.90864629921687 32.5 10.0 38.0 2.0 40.0 20-21 25.379990284707795 32.0 10.0 38.0 2.0 40.0 22-23 25.314120575310326 32.0 10.0 38.0 2.0 40.0 24-25 25.023883002788274 32.0 9.5 38.0 2.0 40.0 26-27 24.68126861709117 31.5 8.0 38.0 2.0 40.0 28-29 24.595923816058267 31.0 5.0 38.0 2.0 40.0 30-31 24.507071562819764 31.0 7.5 38.0 2.0 40.0 32-33 24.196520467249602 31.0 2.0 37.0 2.0 39.5 34-35 21.26589904178056 25.5 2.0 36.0 2.0 39.0 36-37 19.613981175844245 18.0 2.0 35.0 2.0 38.5 38-39 18.68149058806342 18.0 2.0 34.0 2.0 38.0 40-41 20.012190459289165 23.5 2.0 35.0 2.0 38.0 42-43 19.928554029373643 23.5 2.0 35.0 2.0 38.0 44-45 17.948887757290635 17.5 2.0 34.0 2.0 38.0 46-47 18.77437590268431 18.5 2.0 33.0 2.0 38.0 48-49 18.203800578226613 17.0 2.0 33.5 2.0 38.0 50-51 16.229081531873817 11.0 2.0 31.5 2.0 37.5 52-53 19.135944150916522 22.0 2.0 33.5 2.0 38.0 54-55 20.781963749356763 26.5 2.0 35.5 2.0 38.5 56-57 21.00275831783587 28.0 2.0 36.0 2.0 39.0 58-59 20.63542051983878 26.5 2.0 36.0 2.0 39.0 60-61 20.32027670214717 26.0 2.0 35.5 2.0 39.0 62-63 19.846387892225252 26.0 2.0 35.0 2.0 38.5 64-65 19.35049046966006 24.5 2.0 34.0 2.0 38.0 66-67 18.936707612375102 23.0 2.0 34.0 2.0 38.0 68-69 18.479828758790887 22.0 2.0 34.0 2.0 37.5 70-71 17.884789859473017 20.5 2.0 33.5 2.0 36.5 72-73 17.414301825700647 18.5 2.0 33.0 2.0 36.0 74-75 16.875068209094113 14.5 2.0 32.0 2.0 36.0 76-77 16.316138095051052 9.5 2.0 31.5 2.0 35.0 78-79 15.81748193077162 7.0 2.0 31.0 2.0 35.0 80-81 15.318121421936723 3.5 2.0 31.0 2.0 34.5 82-83 14.842128055915772 2.0 2.0 31.0 2.0 34.0 84-85 14.322589665110657 2.0 2.0 30.0 2.0 34.0 86-87 13.759404518712959 2.0 2.0 29.5 2.0 34.0 88-89 13.21029072121774 2.0 2.0 29.0 2.0 34.0 90-91 12.641829278183177 2.0 2.0 29.0 2.0 34.0 92-93 12.015470853982098 2.0 2.0 28.5 2.0 34.0 94-95 11.3606956239645 2.0 2.0 26.5 2.0 33.5 96-97 10.630946766246057 2.0 2.0 25.5 2.0 33.0 98-99 9.83301226901808 2.0 2.0 24.0 2.0 32.5 100-101 8.79281616592973 2.0 2.0 10.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.734913146707321 1101 2 7.861794576211086 1101 3 7.462920953246009 1101 4 9.300522812762988 1101 5 9.237940018574289 1101 6 8.551780576191941 1101 7 8.341208145119353 1101 8 7.4391990036812885 1101 9 7.783606697453848 1101 10-11 7.127351282338633 1101 12-13 6.912477628260344 1101 14-15 7.263317925183298 1101 16-17 8.121065309619851 1101 18-19 8.588612805622464 1101 20-21 9.044562200597905 1101 22-23 8.870097034157169 1101 24-25 8.967984820890617 1101 26-27 8.971536165852243 1101 28-29 8.804051116344837 1101 30-31 8.65250065494762 1101 32-33 8.63894573195087 1101 34-35 11.196455160961047 1101 36-37 5.387442763769087 1101 38-39 8.561946239540756 1101 40-41 9.97906914782871 1101 42-43 10.363405409103837 1101 44-45 4.730839187939889 1101 46-47 6.344547542004314 1101 48-49 9.558799009407721 1101 50-51 -3.629883939282383 1101 52-53 5.8715281631034735 1101 54-55 8.366047225354599 1101 56-57 8.865988251571725 1101 58-59 9.121445748844625 1101 60-61 8.996296157416825 1101 62-63 9.052157368986684 1101 64-65 9.030687698847888 1101 66-67 8.981756797206042 1101 68-69 8.866773885844982 1101 70-71 8.908963299341572 1101 72-73 8.871538811178464 1101 74-75 8.849460747744715 1101 76-77 8.792671104086189 1101 78-79 8.719470012934346 1101 80-81 8.450628629823767 1101 82-83 8.228512679826899 1101 84-85 8.13836436493481 1101 86-87 8.089246192617765 1101 88-89 7.9085490203797075 1101 90-91 7.842095896337867 1101 92-93 7.835477455785016 1101 94-95 7.65563888421085 1101 96-97 7.600146862217358 1101 98-99 7.210222744525874 1101 100-101 6.689260920451073 1102 1 6.671062983828218 1102 2 7.727826270134344 1102 3 7.408653009187585 1102 4 9.111181762501953 1102 5 9.158939282364624 1102 6 8.446928164538715 1102 7 8.172141027014362 1102 8 7.478984920126351 1102 9 7.326299897453588 1102 10-11 6.857610972690278 1102 12-13 6.513736647152509 1102 14-15 6.831170540152112 1102 16-17 7.745067428246088 1102 18-19 8.007742002972623 1102 20-21 8.457358181812577 1102 22-23 8.279937816676181 1102 24-25 8.374624515865417 1102 26-27 8.522056935447111 1102 28-29 8.222096071754613 1102 30-31 8.067987977831567 1102 32-33 8.015229161664855 1102 34-35 10.140938914166991 1102 36-37 0.9428253823574408 1102 38-39 3.4237028021344393 1102 40-41 8.21024699588203 1102 42-43 9.34405003933816 1102 44-45 1.0801106997338117 1102 46-47 1.7609317359648031 1102 48-49 7.654057628143708 1102 50-51 -4.770310591237806 1102 52-53 4.739044578895527 1102 54-55 7.548862184850115 1102 56-57 8.076251949960156 1102 58-59 8.356654862822445 1102 60-61 8.284119333711523 1102 62-63 8.352559969483835 1102 64-65 8.155677338817405 1102 66-67 8.30697490822283 1102 68-69 8.263363946561238 1102 70-71 8.174332485502902 1102 72-73 8.16490792222957 1102 74-75 7.851822106255511 1102 76-77 7.892353860527699 1102 78-79 7.915072730370342 1102 80-81 7.755482158417772 1102 82-83 7.536065320113046 1102 84-85 7.432535649908816 1102 86-87 7.281179759569785 1102 88-89 7.179908084791256 1102 90-91 7.210505460070507 1102 92-93 7.057562272707093 1102 94-95 6.728294182686566 1102 96-97 6.665510075173611 1102 98-99 6.380073825562684 1102 100-101 5.96126991490004 1103 1 6.648200090127997 1103 2 7.705782034737194 1103 3 7.477471042653502 1103 4 9.041778104259702 1103 5 9.005623548184179 1103 6 8.324255858972865 1103 7 8.074293674217607 1103 8 7.354222355041813 1103 9 7.322272706794394 1103 10-11 6.806103601084384 1103 12-13 6.6616798061780536 1103 14-15 7.044705354656795 1103 16-17 7.6583180570845 1103 18-19 8.074579171436017 1103 20-21 8.391713469592982 1103 22-23 8.406161002615804 1103 24-25 8.520054401903057 1103 26-27 8.36452146985701 1103 28-29 8.391025132251784 1103 30-31 8.202123260195865 1103 32-33 8.02922213672835 1103 34-35 10.385512802120118 1103 36-37 -3.115228694865003 1103 38-39 -1.7118042730579255 1103 40-41 6.710192018535576 1103 42-43 9.240805463609629 1103 44-45 0.9015464391383112 1103 46-47 0.7428243427200876 1103 48-49 7.650098618997141 1103 50-51 -5.102731956827077 1103 52-53 4.372649774006689 1103 54-55 7.407659632620835 1103 56-57 8.103887869645536 1103 58-59 8.49006911768814 1103 60-61 8.455136733121385 1103 62-63 8.445914784795164 1103 64-65 8.31594982799319 1103 66-67 8.231409065688197 1103 68-69 8.143199392241062 1103 70-71 8.193534304060694 1103 72-73 8.081653973943805 1103 74-75 7.974494331796116 1103 76-77 7.99564877213821 1103 78-79 7.72272155972898 1103 80-81 7.438182283961384 1103 82-83 7.353794850134356 1103 84-85 6.980646749923094 1103 86-87 7.030331732000125 1103 88-89 7.052212754360598 1103 90-91 6.8617959152349215 1103 92-93 6.85297844241696 1103 94-95 6.501199329718558 1103 96-97 6.2024453289848935 1103 98-99 5.774375995783432 1103 100-101 5.506595175926396 1104 1 6.507502278189705 1104 2 7.58602665583064 1104 3 7.0477346451259315 1104 4 8.98706930492224 1104 5 9.026189719457538 1104 6 8.170564124349031 1104 7 7.897474965068717 1104 8 7.165869146379734 1104 9 7.193362525741001 1104 10-11 6.686461741446546 1104 12-13 6.5394819681469265 1104 14-15 7.003353886340438 1104 16-17 7.571600799654448 1104 18-19 8.021287683906696 1104 20-21 8.253899070239225 1104 22-23 8.281347782534638 1104 24-25 8.269907108625464 1104 26-27 8.20745163956542 1104 28-29 8.1538900961217 1104 30-31 7.699318126114203 1104 32-33 7.723064451709309 1104 34-35 10.107007345629842 1104 36-37 -3.217103264294643 1104 38-39 -1.8798652852066873 1104 40-41 6.2252306390182355 1104 42-43 8.589101802244588 1104 44-45 0.5900401862762017 1104 46-47 0.05940518866521671 1104 48-49 7.134358000915025 1104 50-51 -5.25161813247292 1104 52-53 3.9051426864826766 1104 54-55 6.737922310264402 1104 56-57 7.3030890583671315 1104 58-59 7.714250644146738 1104 60-61 7.497156811016989 1104 62-63 7.707028209265054 1104 64-65 7.670219572934965 1104 66-67 7.477214024994183 1104 68-69 7.4891571413033375 1104 70-71 7.508224345892604 1104 72-73 7.253375085084766 1104 74-75 7.315473753350403 1104 76-77 7.204599764545968 1104 78-79 6.972806207044956 1104 80-81 6.694412334136823 1104 82-83 6.468899991904909 1104 84-85 6.330272472146499 1104 86-87 6.087917266318069 1104 88-89 6.054593300902033 1104 90-91 5.961163140844064 1104 92-93 5.70661119025179 1104 94-95 5.418336979763934 1104 96-97 5.130440338614537 1104 98-99 4.913099012473273 1104 100-101 4.556112311816335 1105 1 5.939385586360633 1105 2 6.912934643450999 1105 3 6.6867398632935 1105 4 8.524867168521258 1105 5 8.44232524097217 1105 6 7.620271062778539 1105 7 7.508084592237239 1105 8 6.625025771238427 1105 9 6.619655853711059 1105 10-11 6.104125862762434 1105 12-13 5.815979462270914 1105 14-15 6.175939011806246 1105 16-17 6.783214122455298 1105 18-19 7.42673337169612 1105 20-21 7.947447393311318 1105 22-23 7.748271895267521 1105 24-25 7.934308937961259 1105 26-27 7.826467628390752 1105 28-29 7.592626466851094 1105 30-31 7.273168252543734 1105 32-33 6.932824886169946 1105 34-35 9.265331163117914 1105 36-37 -3.6533602225497415 1105 38-39 -2.4131085543174358 1105 40-41 5.321804118765474 1105 42-43 7.611757232495318 1105 44-45 -3.3996095953334944 1105 46-47 -4.448851764297178 1105 48-49 4.551096119119688 1105 50-51 -9.822603471234807 1105 52-53 0.29830973620897794 1105 54-55 4.978243273219427 1105 56-57 5.912699135856766 1105 58-59 6.3906278835680475 1105 60-61 6.2370078257390915 1105 62-63 6.375367150662001 1105 64-65 6.152755515925456 1105 66-67 5.999799216618825 1105 68-69 5.966962948372718 1105 70-71 6.036817751797763 1105 72-73 5.994911826832887 1105 74-75 5.99910497784893 1105 76-77 5.716872117520097 1105 78-79 5.535358059029123 1105 80-81 5.34203496063361 1105 82-83 5.1369684539458405 1105 84-85 5.185801983175397 1105 86-87 4.971786353868698 1105 88-89 5.040525176712199 1105 90-91 4.806120691132499 1105 92-93 4.641183193333914 1105 94-95 4.360386364244219 1105 96-97 4.263412973998664 1105 98-99 3.9940004574262566 1105 100-101 3.650832462164484 1106 1 5.866727060206795 1106 2 6.995139901487459 1106 3 6.5642090708564815 1106 4 7.910696045526606 1106 5 7.954062677858801 1106 6 7.057637624100767 1106 7 6.904664629610114 1106 8 6.1984280233704325 1106 9 6.257265683179369 1106 10-11 5.8201151101668565 1106 12-13 5.674932734693929 1106 14-15 5.980939069214667 1106 16-17 6.340023047167037 1106 18-19 6.881797367723454 1106 20-21 7.282000081316692 1106 22-23 7.053797239646229 1106 24-25 7.105340079154381 1106 26-27 7.175121401128074 1106 28-29 7.024796059911562 1106 30-31 6.611445526562694 1106 32-33 6.621904012184281 1106 34-35 9.204905364925704 1106 36-37 -0.2665610716201563 1106 38-39 0.7551420821983292 1106 40-41 6.4129565495811605 1106 42-43 7.500488104730028 1106 44-45 -3.57582335268536 1106 46-47 -4.472351036645556 1106 48-49 4.334280262342194 1106 50-51 -9.785941598170545 1106 52-53 -0.03433254336425051 1106 54-55 4.258270958426142 1106 56-57 5.320157636750903 1106 58-59 5.965664768472795 1106 60-61 5.8080889122507 1106 62-63 5.959055124460804 1106 64-65 5.672275452431752 1106 66-67 5.674977182696196 1106 68-69 5.4861842176153 1106 70-71 5.480625321670974 1106 72-73 5.308419523865734 1106 74-75 5.270019867007637 1106 76-77 5.007284912706972 1106 78-79 4.999392908805662 1106 80-81 4.779525452365368 1106 82-83 4.780145464173145 1106 84-85 4.673343998956968 1106 86-87 4.427838348667496 1106 88-89 4.237016876891028 1106 90-91 3.9415597650199956 1106 92-93 3.674579675920505 1106 94-95 3.6988958552899422 1106 96-97 3.501360436690371 1106 98-99 3.2309637246538028 1106 100-101 2.8505794345883544 1107 1 5.929270450768762 1107 2 7.157639510581497 1107 3 6.610876988422234 1107 4 8.007854940982714 1107 5 8.362662609059353 1107 6 7.085051077520411 1107 7 7.071529876506322 1107 8 6.518184410773877 1107 9 6.598994112988301 1107 10-11 5.97085314062625 1107 12-13 5.783596774472517 1107 14-15 6.115697645691508 1107 16-17 6.851201394103896 1107 18-19 7.1655466250021185 1107 20-21 7.632553604161242 1107 22-23 7.3914125569055145 1107 24-25 7.408479835854511 1107 26-27 7.493812960686505 1107 28-29 7.235432611182787 1107 30-31 7.112285974384566 1107 32-33 6.7689229633054 1107 34-35 9.118340021084823 1107 36-37 -0.2313271534914989 1107 38-39 0.7905495625748493 1107 40-41 6.834691702972663 1107 42-43 7.892358824309728 1107 44-45 -3.253304823742681 1107 46-47 -4.294601404097161 1107 48-49 4.656110796788827 1107 50-51 -9.682667369818919 1107 52-53 0.4179847471883349 1107 54-55 5.281443082140061 1107 56-57 6.2980605044370535 1107 58-59 6.609128038948448 1107 60-61 6.448718114489807 1107 62-63 6.474359874750071 1107 64-65 6.665954502999348 1107 66-67 6.584498870159059 1107 68-69 6.383343894883335 1107 70-71 6.316878722552861 1107 72-73 6.256886390676254 1107 74-75 6.160739759430317 1107 76-77 6.0675438488173015 1107 78-79 5.905842075394146 1107 80-81 5.795321962356921 1107 82-83 5.514585843195569 1107 84-85 5.429852583251931 1107 86-87 5.2792565555036575 1107 88-89 5.211988731661643 1107 90-91 5.221392144542776 1107 92-93 4.9712413389908345 1107 94-95 4.788994207230401 1107 96-97 4.613687265348469 1107 98-99 4.177769241118764 1107 100-101 3.911198004185252 1108 1 6.12393150042444 1108 2 6.525880769263143 1108 3 6.711796417483445 1108 4 8.147316148113095 1108 5 8.141905087549947 1108 6 7.541654087829112 1108 7 7.548613897629011 1108 8 6.447006155922153 1108 9 4.876765690974697 1108 10-11 5.720821366695443 1108 12-13 5.266157536302881 1108 14-15 5.978936060218853 1108 16-17 5.848724189961814 1108 18-19 6.630259349918926 1108 20-21 7.466978488263887 1108 22-23 7.559008804791773 1108 24-25 7.271817059556934 1108 26-27 7.540116490072741 1108 28-29 7.257799521036272 1108 30-31 7.126683439909851 1108 32-33 7.197342109501129 1108 34-35 9.319936312369617 1108 36-37 -3.6427670085403783 1108 38-39 -2.30706189029976 1108 40-41 5.52078890330187 1108 42-43 7.789469411538466 1108 44-45 -3.248703803567011 1108 46-47 -4.210015712905669 1108 48-49 5.037126725602821 1108 50-51 -9.789976458868072 1108 52-53 0.5957168790021008 1108 54-55 5.423062979130528 1108 56-57 6.493509315084577 1108 58-59 6.820041454212028 1108 60-61 6.724023337969037 1108 62-63 6.712663580830366 1108 64-65 6.635721220349307 1108 66-67 6.747646380245346 1108 68-69 6.593438937685146 1108 70-71 6.374536782359147 1108 72-73 6.3785092480671 1108 74-75 6.237905462804399 1108 76-77 6.0907175065237205 1108 78-79 6.115372937180647 1108 80-81 5.9420989233121855 1108 82-83 5.975466709554418 1108 84-85 5.846476238865916 1108 86-87 5.700447132440743 1108 88-89 5.599332322549811 1108 90-91 5.513609623924594 1108 92-93 5.335409722224744 1108 94-95 5.0218947951256165 1108 96-97 4.721440026968041 1108 98-99 4.5337263881607655 1108 100-101 4.270423216256866 1109 1 4.837482122138326 1109 2 6.338492831237449 1109 3 6.12846046488729 1109 4 7.6544077668118575 1109 5 7.52917208642409 1109 6 5.122474910996878 1109 7 4.900937311176914 1109 8 3.4729241155135853 1109 9 3.0505146319430274 1109 10-11 2.740694775599465 1109 12-13 3.231400980500407 1109 14-15 3.940812406661532 1109 16-17 3.228695975545847 1109 18-19 4.0058486840541825 1109 20-21 4.499513307469623 1109 22-23 5.862320395412034 1109 24-25 5.851353665241497 1109 26-27 4.540817673809638 1109 28-29 4.688010643530557 1109 30-31 4.583566338757034 1109 32-33 4.279165827166683 1109 34-35 6.527352974505668 1109 36-37 -4.712095745279829 1109 38-39 -3.238607774970646 1109 40-41 3.249085514570421 1109 42-43 4.035718662570883 1109 44-45 -4.589526989734585 1109 46-47 -6.100656003737289 1109 48-49 1.9324483116131823 1109 50-51 -10.061861001479656 1109 52-53 -1.1527830267044372 1109 54-55 2.5131842028614457 1109 56-57 3.8580422807017207 1109 58-59 4.457601385777057 1109 60-61 4.82026825659916 1109 62-63 4.785945493079112 1109 64-65 4.267359494206971 1109 66-67 4.525058310718503 1109 68-69 4.583472713433206 1109 70-71 4.672847315363558 1109 72-73 4.625393705729621 1109 74-75 4.593999482163724 1109 76-77 4.6134035081638025 1109 78-79 4.634484777721058 1109 80-81 4.44864095695881 1109 82-83 4.364011271568366 1109 84-85 4.169057639891198 1109 86-87 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2313 90-91 -10.408616081186784 2313 92-93 -9.811688604227138 2313 94-95 -9.280649599255513 2313 96-97 -8.53925355964829 2313 98-99 -7.71415087978426 2313 100-101 -6.667234833873021 2314 1 -20.756534971878768 2314 2 -19.626517852523236 2314 3 -20.046486116309293 2314 4 -21.975132831478742 2314 5 -21.9935309050289 2314 6 -22.754661841139875 2314 7 -22.83410542386582 2314 8 -23.62322973064026 2314 9 -24.76171827403988 2314 10-11 -25.017318044913356 2314 12-13 -24.970789190439763 2314 14-15 -25.202617080517957 2314 16-17 -24.36211599026612 2314 18-19 -23.75617054671505 2314 20-21 -23.25062023953892 2314 22-23 -23.168528963562323 2314 24-25 -22.87594334330551 2314 26-27 -22.533167368925405 2314 28-29 -22.456353672708758 2314 30-31 -22.358474259533562 2314 32-33 -22.05603716428631 2314 34-35 -19.234132068229375 2314 36-37 -17.5370156170747 2314 38-39 -16.644724227962097 2314 40-41 -17.970332220472315 2314 42-43 -17.898709756232538 2314 44-45 -15.902427630760226 2314 46-47 -16.69080023450652 2314 48-49 -16.15542562001075 2314 50-51 -14.17150309549675 2314 52-53 -17.02898494978136 2314 54-55 -18.64293225013001 2314 56-57 -18.844815625281182 2314 58-59 -18.45666143473576 2314 60-61 -18.167044777588874 2314 62-63 -17.69655984880123 2314 64-65 -17.20406144596397 2314 66-67 -16.7740869884268 2314 68-69 -16.326783696823203 2314 70-71 -15.72458858207234 2314 72-73 -15.253746251535336 2314 74-75 -14.735060346896105 2314 76-77 -14.165709739699444 2314 78-79 -13.679617786381504 2314 80-81 -13.178362248169394 2314 82-83 -12.72145881390172 2314 84-85 -12.21368808660453 2314 86-87 -11.661466464166729 2314 88-89 -11.110977775515389 2314 90-91 -10.548012427493376 2314 92-93 -9.937185029961848 2314 94-95 -9.280649599255513 2314 96-97 -8.563479135502142 2314 98-99 -7.774383868929084 2314 100-101 -6.738190618887582 2315 1 -20.756534971878768 2315 2 -19.626517852523236 2315 3 -20.046486116309293 2315 4 -16.890744645824732 2315 5 -21.9935309050289 2315 6 -22.754661841139875 2315 7 -22.83410542386582 2315 8 -23.62322973064026 2315 9 -20.05074780990486 2315 10-11 -18.497143994280442 2315 12-13 -15.028014928836388 2315 14-15 -14.885502101615003 2315 16-17 -20.34352421811422 2315 18-19 -23.75617054671505 2315 20-21 -23.25062023953892 2315 22-23 -23.168528963562323 2315 24-25 -22.87594334330551 2315 26-27 -22.533167368925405 2315 28-29 -22.456353672708758 2315 30-31 -22.358474259533562 2315 32-33 -22.05603716428631 2315 34-35 -19.234132068229375 2315 36-37 -17.5370156170747 2315 38-39 -16.644724227962097 2315 40-41 -17.970332220472315 2315 42-43 -17.898709756232538 2315 44-45 -15.902427630760226 2315 46-47 -16.69080023450652 2315 48-49 -16.15542562001075 2315 50-51 -14.17150309549675 2315 52-53 -17.02898494978136 2315 54-55 -18.64293225013001 2315 56-57 -18.844815625281182 2315 58-59 -18.45666143473576 2315 60-61 -18.167044777588874 2315 62-63 -17.69655984880123 2315 64-65 -17.20406144596397 2315 66-67 -16.7740869884268 2315 68-69 -16.326783696823203 2315 70-71 -15.72458858207234 2315 72-73 -15.253746251535336 2315 74-75 -14.735060346896105 2315 76-77 -14.165709739699444 2315 78-79 -13.679617786381504 2315 80-81 -13.178362248169394 2315 82-83 -12.72145881390172 2315 84-85 -12.21368808660453 2315 86-87 -11.661466464166729 2315 88-89 -11.110977775515389 2315 90-91 -10.548012427493376 2315 92-93 -9.937185029961848 2315 94-95 -9.280649599255513 2315 96-97 -8.563479135502142 2315 98-99 -7.774383868929084 2315 100-101 -6.738190618887582 2316 1 -20.756534971878768 2316 2 -19.626517852523236 2316 3 -20.046486116309293 2316 4 -21.975132831478742 2316 5 -21.9935309050289 2316 6 -22.754661841139875 2316 7 -22.83410542386582 2316 8 -23.62322973064026 2316 9 -24.76171827403988 2316 10-11 -25.017318044913356 2316 12-13 -24.970789190439763 2316 14-15 -25.202617080517957 2316 16-17 -24.36211599026612 2316 18-19 -23.75617054671505 2316 20-21 -23.25062023953892 2316 22-23 -23.168528963562323 2316 24-25 -22.87594334330551 2316 26-27 -22.533167368925405 2316 28-29 -22.456353672708758 2316 30-31 -22.358474259533562 2316 32-33 -22.05603716428631 2316 34-35 -19.234132068229375 2316 36-37 -17.5370156170747 2316 38-39 -16.644724227962097 2316 40-41 -17.970332220472315 2316 42-43 -17.898709756232538 2316 44-45 -15.902427630760226 2316 46-47 -16.69080023450652 2316 48-49 -16.15542562001075 2316 50-51 -14.17150309549675 2316 52-53 -17.02898494978136 2316 54-55 -18.64293225013001 2316 56-57 -18.844815625281182 2316 58-59 -18.45666143473576 2316 60-61 -18.167044777588874 2316 62-63 -17.69655984880123 2316 64-65 -17.20406144596397 2316 66-67 -16.7740869884268 2316 68-69 -16.326783696823203 2316 70-71 -15.72458858207234 2316 72-73 -15.253746251535336 2316 74-75 -14.735060346896105 2316 76-77 -14.165709739699444 2316 78-79 -13.679617786381504 2316 80-81 -13.178362248169394 2316 82-83 -12.72145881390172 2316 84-85 -12.21368808660453 2316 86-87 -11.661466464166729 2316 88-89 -11.110977775515389 2316 90-91 -10.548012427493376 2316 92-93 -9.937185029961848 2316 94-95 -9.280649599255513 2316 96-97 -8.563479135502142 2316 98-99 -7.774383868929084 2316 100-101 -6.738190618887582 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 592819.0 3 34507.0 4 73466.0 5 43648.0 6 37995.0 7 47393.0 8 61168.0 9 73005.0 10 76237.0 11 72015.0 12 65096.0 13 60525.0 14 57836.0 15 60849.0 16 66085.0 17 69279.0 18 71230.0 19 74598.0 20 77835.0 21 82708.0 22 87260.0 23 92670.0 24 98139.0 25 103761.0 26 110313.0 27 117769.0 28 124839.0 29 131556.0 30 137336.0 31 137032.0 32 133076.0 33 130260.0 34 122085.0 35 103793.0 36 73881.0 37 32396.0 38 4269.0 39 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.0248548311433 20.316147351984128 14.057682967461954 23.60131484941062 2 15.04933291425343 21.182174250674645 40.91870368314087 22.849789151931052 3 18.105475368319386 26.62356859952889 30.711780046891356 24.559175985260364 4 12.341454796565117 17.45291110325909 39.428804754999916 30.77682934517587 5 12.760444401014933 37.97672885748301 35.8203072247554 13.442519516746652 6 29.51949264991259 38.26959509664537 18.571171184595805 13.639741068846236 7 25.74575884092348 33.283616077271766 23.637767217730197 17.33285786407456 8 24.73435003987169 35.959585076375575 21.472774992064373 17.833289891688366 9 25.898913706851385 16.47970537405289 21.520804573423685 36.10057634567204 10-11 24.115044683406893 26.756396483125144 29.461150056063463 19.667408777404493 12-13 24.738889768257838 24.775769475133117 29.338378938841448 21.146961817767597 14-15 21.98494820638989 26.081758738331008 27.178537600644777 24.75475545463433 16-17 21.532087780144924 29.026764103317543 27.741663525039108 21.69948459149843 18-19 21.491735193952984 27.931589002408835 29.76875155743833 20.80792424619985 20-21 22.217557872034387 27.18509082207836 29.709634181916673 20.88771712397058 22-23 21.941237425825815 27.11053868136953 29.508698868283588 21.439525024521068 24-25 21.760250401538574 27.263260177516663 29.45776674017851 21.518722680766253 26-27 21.691892919358263 27.449904780038793 29.32616675833823 21.53203554226472 28-29 21.76836017425346 27.564736829403675 29.308135632797168 21.358767363545702 30-31 21.94555937233345 27.36184345044923 29.215749626692983 21.476847550524344 32-33 21.807562142777037 27.526042412587937 29.089096922876006 21.577298521759015 34-35 22.21709647951655 27.477513878153324 28.97862061696297 21.326769025367156 36-37 22.687833712050104 27.441004706174628 28.54590504902771 21.32525653274756 38-39 22.404744518075166 27.51430923440178 28.582666897107238 21.498279350415817 40-41 22.359159296678975 27.46029226695531 28.722283945959493 21.458264490406222 42-43 22.523506142081185 27.54574629553183 28.306569935083637 21.624177627303347 44-45 22.740287436254057 27.66928140936486 28.091719981455725 21.498711172925358 46-47 22.163579461555333 27.669657447460676 28.311768560813373 21.854994530170615 48-49 22.654214143779885 27.775011408258603 27.723913989872432 21.84686045808908 50-51 22.881928987620324 27.883938499912592 27.58905383510465 21.645078677362438 52-53 22.035264747735276 27.98176553259864 28.065102821815955 21.917866897850125 54-55 21.777891041256854 27.872331070958666 28.2699510180686 22.07982686971588 56-57 21.8155635740957 28.09886659233884 28.044513152172467 22.04105668139299 58-59 21.882029887885604 27.97766528773592 28.140839396985058 21.99946542739342 60-61 21.80693774698588 28.03162070260235 28.185144382730808 21.976297167680965 62-63 21.844183705539134 27.953565425951748 28.168211948108734 22.034038920400384 64-65 21.653084924999888 27.817646439556153 28.130276643792186 22.398991991651773 66-67 21.65478936351074 27.991524720051153 28.03827574965615 22.31541016678196 68-69 21.79615982972984 27.87217245216957 28.06205193462207 22.26961578347852 70-71 21.723098027331297 27.7443895176198 27.89047857113903 22.64203388390987 72-73 21.738996431007326 27.937695854241884 27.8262137393682 22.49709397538259 74-75 21.71817340593341 28.066088018270413 27.716709471305446 22.49902910449073 76-77 21.799980939418052 27.81728833159784 27.761369829017625 22.621360899966483 78-79 21.645208951636434 27.886990795665312 27.744751626921747 22.723048625776507 80-81 21.728123368539414 27.986554756837073 27.57818561503554 22.70713625958797 82-83 21.85597373279791 27.889592745321124 27.62819644446718 22.626237077413787 84-85 21.762597021207185 27.952535053204898 27.53725384038499 22.747614085202926 86-87 21.681066704412398 27.955778098082867 27.494014289875825 22.869140907628914 88-89 21.698408910844346 27.8293192933731 27.397866065639214 23.07440573014334 90-91 21.68180591976287 27.85228349469059 27.393589011677914 23.072321573868628 92-93 21.682703154886784 27.637390089285812 27.51370535729255 23.16620139853485 94-95 21.549328039217976 27.563188803962724 27.543558075862535 23.34392508095677 96-97 21.598657709369974 27.4153034424978 27.425352813211617 23.560686034920607 98-99 21.45317936070815 27.451958172230757 27.343175236101203 23.751687230959888 100-101 21.710907674581872 27.565480172567668 27.16985498511419 23.553757167736276 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 44429.0 1 47922.5 2 35897.5 3 21989.5 4 28320.0 5 35149.0 6 36943.5 7 34947.0 8 31765.5 9 28690.0 10 25371.5 11 22023.0 12 18855.5 13 15943.0 14 13564.5 15 11852.5 16 10728.0 17 10145.0 18 10112.5 19 10590.5 20 11370.5 21 12676.5 22 14622.5 23 17040.0 24 20166.5 25 24232.0 26 29055.0 27 34756.0 28 41548.0 29 49771.5 30 58391.5 31 66393.5 32 74447.5 33 81027.0 34 85779.5 35 89952.5 36 94779.5 37 99823.5 38 103444.5 39 105413.5 40 106231.5 41 106854.0 42 107157.5 43 107821.0 44 108325.0 45 108202.5 46 107602.5 47 106302.0 48 104349.0 49 101157.5 50 97359.0 51 92871.5 52 88049.5 53 83161.5 54 77675.0 55 71314.0 56 63961.5 57 55915.0 58 48655.5 59 41902.0 60 35042.0 61 29028.5 62 23541.5 63 18516.0 64 14503.0 65 11252.5 66 8501.0 67 6445.5 68 4849.5 69 3687.0 70 2836.5 71 2174.5 72 1622.0 73 1209.5 74 913.5 75 686.5 76 481.5 77 309.5 78 204.0 79 148.5 80 84.5 81 42.5 82 31.0 83 21.0 84 9.0 85 6.5 86 6.0 87 4.5 88 3.5 89 2.0 90 1.5 91 1.0 92 1.0 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 14.763287951031309 2 21.8711065005704 3 17.60720095875635 4 22.903758981629394 5 22.786401190702595 6 16.559430149224966 7 15.367427660211188 8 12.400053013054606 9 3.135202222479061 10-11 6.713710571893869 12-13 3.684138838816294 14-15 7.6477689444665655 16-17 11.812713016541712 18-19 14.948805383594383 20-21 15.384170855762386 22-23 13.280420125631897 24-25 15.8658521043159 26-27 13.327922761448885 28-29 13.349710109583862 30-31 14.243740234902088 32-33 12.688982638224616 34-35 19.570492526883072 36-37 24.357421587447096 38-39 23.39812832267656 40-41 18.54562527852341 42-43 18.47131940054556 44-45 23.807863282836234 46-47 13.155178197053141 48-49 21.911742306819555 50-51 21.602141546551312 52-53 11.600123885517801 54-55 13.231532820478186 56-57 12.734153943015489 58-59 13.782136691731065 60-61 13.18506162202427 62-63 13.14285746581209 64-65 14.238229816113085 66-67 12.536400554885043 68-69 13.59298803447883 70-71 12.161720335790791 72-73 11.522582402661278 74-75 11.591547413197482 76-77 11.639106566130252 78-79 12.059028736410106 80-81 12.041706240422116 82-83 10.509696782847959 84-85 10.4333986765387 86-87 10.560590445612025 88-89 12.009095864614379 90-91 9.441537423797266 92-93 11.51260713172529 94-95 11.614309681579744 96-97 11.141657042272826 98-99 11.002752100953133 100-101 11.10477962422335 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3538751.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.90376425265309 #Duplication Level Percentage of deduplicated Percentage of total 1 98.8652907191629 95.80418824619774 2 0.7760253711040443 1.5039955923108788 3 0.15996602571207674 0.4650393013211079 4 0.0675187245963142 0.2617127428368416 5 0.03378061285234654 0.16367342720769662 6 0.02104643095252614 0.12236870300300007 7 0.013999262660613278 0.09496068740025267 8 0.011856820053878338 0.09191763962297249 9 0.007633361467608799 0.06657313141012042 >10 0.03925229672697463 0.6471624389787913 >50 0.0021244983851156168 0.14789742215889173 >100 0.0013891621523376466 0.2796448670140786 >500 0.0 0.0 >1k 8.75356299274904E-5 0.16597487703733316 >5k 2.917854330916346E-5 0.1848909235003614 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6539 0.1847827100578707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.477567367695552E-5 2 0.0 0.0 0.0 0.0 8.477567367695552E-5 3 0.0 0.0 0.0 0.0 8.477567367695552E-5 4 0.0 0.0 0.0 0.0 8.477567367695552E-5 5 2.8258557892318503E-5 0.0 0.0 0.0 8.477567367695552E-5 6 2.8258557892318503E-5 0.0 0.0 0.0 1.412927894615925E-4 7 2.8258557892318503E-5 0.0 0.0 0.0 1.412927894615925E-4 8 2.8258557892318503E-5 0.0 0.0 0.0 1.6955134735391103E-4 9 2.8258557892318503E-5 0.0 0.0 0.0 1.6955134735391103E-4 10-11 5.6517115784637006E-5 2.8258557892318503E-5 0.0 2.8258557892318503E-5 1.6955134735391103E-4 12-13 5.6517115784637006E-5 2.8258557892318503E-5 0.0 4.238783683847776E-5 3.2497341576166283E-4 14-15 5.6517115784637006E-5 2.8258557892318503E-5 0.0 8.477567367695552E-5 3.532319736539813E-4 16-17 7.064639473079625E-5 2.8258557892318503E-5 0.0 8.477567367695552E-5 3.6736125260014053E-4 18-19 8.477567367695552E-5 2.8258557892318503E-5 0.0 8.477567367695552E-5 3.9561981049245906E-4 20-21 8.477567367695552E-5 2.8258557892318503E-5 0.0 9.890495262311476E-5 3.9561981049245906E-4 22-23 8.477567367695552E-5 2.8258557892318503E-5 0.0 1.6955134735391103E-4 4.0974908943861835E-4 24-25 8.477567367695552E-5 5.6517115784637006E-5 0.0 2.5432702103086655E-4 4.238783683847776E-4 26-27 8.477567367695552E-5 5.6517115784637006E-5 0.0 5.227833210078923E-4 4.380076473309368E-4 28-29 8.477567367695552E-5 5.6517115784637006E-5 0.0 0.00113034231569274 4.5213692627709605E-4 30-31 1.1303423156927401E-4 5.6517115784637006E-5 0.0 0.003080182810262717 4.5213692627709605E-4 32-33 1.1303423156927401E-4 5.6517115784637006E-5 0.0 0.009523134009711337 4.5213692627709605E-4 34-35 1.1303423156927401E-4 5.6517115784637006E-5 0.0 0.017633340124806746 4.5213692627709605E-4 36-37 1.1303423156927401E-4 5.6517115784637006E-5 0.0 0.03005297631848073 4.803954841694146E-4 38-39 1.1303423156927401E-4 5.6517115784637006E-5 0.0 0.04998938891151143 5.086540420617331E-4 40-41 1.412927894615925E-4 5.6517115784637006E-5 0.0 0.07040619698871156 5.510418789002108E-4 42-43 1.412927894615925E-4 5.6517115784637006E-5 0.0 0.09137404694481188 5.6517115784637E-4 44-45 1.412927894615925E-4 5.6517115784637006E-5 0.0 0.117824057132022 5.6517115784637E-4 46-47 1.412927894615925E-4 5.6517115784637006E-5 0.0 0.15181910227648115 5.934297157386886E-4 48-49 1.412927894615925E-4 5.6517115784637006E-5 0.0 0.1881454784470566 6.216882736310071E-4 50-51 1.412927894615925E-4 5.6517115784637006E-5 0.0 0.2313810720223039 6.499468315233256E-4 52-53 1.412927894615925E-4 5.6517115784637006E-5 0.0 0.2898480283015109 6.640761104694849E-4 54-55 1.412927894615925E-4 7.064639473079625E-5 0.0 0.3538536619276123 7.064639473079626E-4 56-57 1.412927894615925E-4 8.477567367695552E-5 0.0 0.42524890844255503 7.347225052002811E-4 58-59 1.412927894615925E-4 9.890495262311476E-5 0.0 0.5051217223251933 7.347225052002811E-4 60-61 1.412927894615925E-4 1.1303423156927401E-4 0.0 0.5915787801967418 7.629810630925995E-4 62-63 1.412927894615925E-4 1.1303423156927401E-4 1.4129278946159252E-5 0.6821333289626763 9.042738525541921E-4 64-65 1.412927894615925E-4 1.412927894615925E-4 2.8258557892318503E-5 0.7815893234646913 9.184031315003513E-4 66-67 1.5542206840775177E-4 1.412927894615925E-4 2.8258557892318503E-5 0.888562094366063 9.466616893926699E-4 68-69 1.9780990524622953E-4 1.412927894615925E-4 2.8258557892318503E-5 1.004521086677192 9.607909683388291E-4 70-71 1.9780990524622953E-4 1.412927894615925E-4 5.6517115784637006E-5 1.1337192133608722 9.607909683388291E-4 72-73 1.9780990524622953E-4 1.412927894615925E-4 5.6517115784637006E-5 1.2689646714335086 0.0010314373630696254 74-75 1.9780990524622953E-4 1.412927894615925E-4 5.6517115784637006E-5 1.412998541010656 0.0010879544788542624 76-77 1.9780990524622953E-4 1.412927894615925E-4 5.6517115784637006E-5 1.5729843665180172 0.0011020837578004216 78-79 1.9780990524622953E-4 1.412927894615925E-4 5.6517115784637006E-5 1.745898482261114 0.0011020837578004216 80-81 1.9780990524622953E-4 1.412927894615925E-4 5.6517115784637006E-5 1.9371806606342181 0.0011162130367465808 82-83 1.9780990524622953E-4 1.412927894615925E-4 5.6517115784637006E-5 2.1366578207960947 0.00113034231569274 84-85 2.2606846313854802E-4 1.412927894615925E-4 5.6517115784637006E-5 2.339639042136618 0.00113034231569274 86-87 2.2606846313854802E-4 1.412927894615925E-4 5.6517115784637006E-5 2.5543758235603464 0.001172730152531218 88-89 2.5432702103086655E-4 1.6955134735391103E-4 5.6517115784637006E-5 2.7859970933247356 0.0012151179893696957 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2975 0.0 53.9419 1 GTATCAA 6425 0.0 49.011448 1 TCAACGC 7780 0.0 40.181393 4 ATCAACG 7750 0.0 40.15862 3 TATCAAC 7985 0.0 38.921005 2 CAACGCA 8285 0.0 36.90897 5 AACGCAG 8980 0.0 34.068016 6 ACGCAGA 10335 0.0 29.221344 7 CGCAGAG 10580 0.0 28.278425 8 GTACATG 6755 0.0 27.641554 1 TACATGG 7100 0.0 26.22082 2 GCAGAGT 11125 0.0 25.30225 9 ACATGGG 7420 0.0 24.949247 3 AGAGTAC 10180 0.0 20.380873 10-11 CAGAGTA 10715 0.0 20.229343 10-11 CATGGGG 5320 0.0 19.808315 4 GAGTACT 7525 0.0 19.743757 12-13 GTGGTAT 1490 0.0 18.96653 1 AGTACTT 8165 0.0 18.785866 12-13 GTATAGG 960 0.0 17.872849 1 >>END_MODULE