FastQCFastQC Report
Sat 18 Jun 2016
SRR1930919_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930919_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3538751
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76830.21711050028668308No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63050.17817020751106818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41290.1166795855373831No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43850.058.4747731
GTATCAA85400.042.3683431
ATCAACG110700.031.7790783
TCAACGC111050.031.5916294
CAACGCA112350.031.2163775
TATCAAC114750.030.9470752
AACGCAG119900.029.4881956
TAACGCA4150.026.3103054
ACGCAGA135950.025.937067
CGCAGAG141500.024.7855828
GTACATG93700.024.3246151
TACATGG97600.022.5217382
TATAACG4300.022.0816862
ACATGGG97750.021.9529233
GCAGAGT157250.021.6391229
GTGGTAT22200.020.9612641
AGAGTAC149150.020.34831410-11
TGGTATC21000.020.3466952
ATAACGC4950.019.182073
GAGTACT109300.018.95287912-13
Produced by FastQC (version 0.11.5)