##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930919_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3538751 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.221719753664498 31.0 31.0 33.0 30.0 34.0 2 31.331216578956813 31.0 31.0 34.0 30.0 34.0 3 31.41311553144033 31.0 31.0 34.0 28.0 34.0 4 35.29235625790003 37.0 35.0 37.0 33.0 37.0 5 35.076880797773 35.0 35.0 37.0 32.0 37.0 6 35.11184963282243 37.0 35.0 37.0 32.0 37.0 7 34.99268301160494 36.0 35.0 37.0 32.0 37.0 8 34.96739414556153 36.0 35.0 37.0 32.0 37.0 9 36.461892345632684 38.0 35.0 39.0 32.0 39.0 10-11 36.393531926942586 38.0 35.0 39.0 32.0 39.0 12-13 36.29400302536121 38.0 35.0 39.0 32.0 39.0 14-15 37.295390944432086 39.0 36.0 41.0 32.0 41.0 16-17 37.26089007110136 39.0 36.0 40.5 32.0 41.0 18-19 37.264989963973164 39.0 36.0 40.0 31.5 41.0 20-21 37.23631487493751 39.0 36.0 40.0 31.0 41.0 22-23 37.10911674768866 39.0 36.0 40.0 31.0 41.0 24-25 36.986698272921714 39.0 36.0 40.0 31.0 41.0 26-27 36.70593706649606 39.0 35.5 40.0 30.0 41.0 28-29 36.51330963947449 38.0 35.0 40.0 30.0 41.0 30-31 36.26240896858807 38.0 35.0 40.0 30.0 41.0 32-33 36.01154743580433 38.0 34.5 40.0 29.5 41.0 34-35 36.17388437332833 38.0 35.0 40.0 30.0 41.0 36-37 36.206658931357424 38.0 35.0 40.0 30.0 41.0 38-39 36.15874937230679 38.0 35.0 40.0 30.0 41.0 40-41 36.05334481007564 38.0 35.0 40.0 29.5 41.0 42-43 35.900533125953196 38.0 35.0 40.0 29.0 41.0 44-45 35.69976892977212 38.0 34.5 40.0 28.5 41.0 46-47 35.457624314341416 38.0 34.0 40.0 27.0 41.0 48-49 35.24501017449377 38.0 34.0 40.0 27.0 41.0 50-51 34.97045101506153 38.0 33.5 40.0 26.0 41.0 52-53 34.760379297667455 38.0 33.0 40.0 26.0 41.0 54-55 34.59141728253839 38.0 33.0 40.0 26.0 41.0 56-57 34.34033236585451 37.5 33.0 40.0 25.0 41.0 58-59 34.09578506653901 37.0 33.0 40.0 24.5 41.0 60-61 33.78561079883835 37.0 32.0 40.0 23.5 41.0 62-63 33.486722151403136 36.0 32.0 39.0 23.0 41.0 64-65 33.18129150652307 36.0 31.5 39.0 22.5 40.5 66-67 32.84390198688746 35.5 31.0 39.0 22.0 40.0 68-69 32.47982805232694 35.0 31.0 38.5 22.0 40.0 70-71 32.078294008253195 35.0 31.0 38.0 20.5 40.0 72-73 31.65486608128122 35.0 30.0 37.5 20.0 39.5 74-75 31.130339065958584 34.0 30.0 37.0 18.0 39.0 76-77 29.764686467061402 33.0 28.5 35.5 17.0 38.0 78-79 30.528926166322524 34.0 29.5 36.0 18.0 38.5 80-81 30.52822351728053 34.0 30.0 36.0 18.0 37.5 82-83 30.23828209444519 34.0 30.0 35.0 17.5 37.0 84-85 29.90556018210945 34.0 29.0 35.0 14.0 37.0 86-87 29.559813617855568 34.0 29.0 35.0 9.5 36.0 88-89 29.211087188671936 34.0 29.0 35.0 7.0 36.0 90-91 28.86240441896025 34.0 29.0 35.0 2.0 35.5 92-93 28.50278685897934 33.0 29.0 35.0 2.0 35.0 94-95 28.084339926714257 33.0 27.5 35.0 2.0 35.0 96-97 27.61801678049685 33.0 27.0 35.0 2.0 35.0 98-99 27.122417485717417 33.0 26.5 35.0 2.0 35.0 100-101 25.90494329779066 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.47973907890339973 1101 2 0.5613771169230581 1101 3 0.5685061999736263 1101 4 0.34612480777212085 1101 5 0.45696841387648135 1101 6 0.3965152845947131 1101 7 0.49958858567283215 1101 8 0.47422708310233475 1101 9 0.6391699376646045 1101 10-11 0.603806150696748 1101 12-13 0.5569903521004349 1101 14-15 0.7430423264538604 1101 16-17 0.7175076094469333 1101 18-19 0.7291640251411096 1101 20-21 0.7933918356383103 1101 22-23 0.7548539669764622 1101 24-25 0.6313964280194426 1101 26-27 0.7197048685954428 1101 28-29 0.671543911203706 1101 30-31 0.8215057353883353 1101 32-33 0.8025122919849181 1101 34-35 0.8611913809914284 1101 36-37 0.8293147232158944 1101 38-39 0.9052912566056932 1101 40-41 0.8062285940178455 1101 42-43 0.678874826057374 1101 44-45 0.8093175225315861 1101 46-47 0.9422274740185443 1101 48-49 1.028241292270451 1101 50-51 0.9794461494458346 1101 52-53 0.8950696291656683 1101 54-55 0.871696704468178 1101 56-57 1.0390470185036946 1101 58-59 0.9077406861446775 1101 60-61 0.8734612646162532 1101 62-63 0.9036425059403257 1101 64-65 0.8072282653601732 1101 66-67 0.9519227964947277 1101 68-69 0.8256141806410682 1101 70-71 0.7049479448286249 1101 72-73 0.8156024889857356 1101 74-75 0.8861229935826813 1101 76-77 1.0134054201277145 1101 78-79 0.8719132185484568 1101 80-81 0.6992964816163543 1101 82-83 0.8723840250301507 1101 84-85 0.9129338443369939 1101 86-87 0.8312292356046953 1101 88-89 0.8261322046830948 1101 90-91 0.8430403738467191 1101 92-93 1.0175075777680291 1101 94-95 1.015784048188351 1101 96-97 0.9722183005345073 1101 98-99 1.0490039256051453 1101 100-101 1.2672107449095726 1102 1 0.43210203998141594 1102 2 0.566383439779166 1102 3 0.5197609615407615 1102 4 0.2794649722505582 1102 5 0.3816613522854482 1102 6 0.4078803999115692 1102 7 0.4369115343706085 1102 8 0.4571815954459879 1102 9 0.6896556612188505 1102 10-11 0.6312450151856055 1102 12-13 0.6672154917504116 1102 14-15 0.7528778803949763 1102 16-17 0.6966401608569655 1102 18-19 0.6452273819075387 1102 20-21 0.7579921780373198 1102 22-23 0.8055958584719818 1102 24-25 0.7491292713233264 1102 26-27 0.9650839712484611 1102 28-29 0.9136170310038594 1102 30-31 0.877192275183468 1102 32-33 0.8865518070836913 1102 34-35 0.9334185997700146 1102 36-37 0.9060842487977325 1102 38-39 0.8660668052502558 1102 40-41 0.8535990240303377 1102 42-43 0.9174533780747609 1102 44-45 0.9749073935297758 1102 46-47 1.0432206933785935 1102 48-49 0.9029254732161505 1102 50-51 0.9392949609884624 1102 52-53 0.8265421553360781 1102 54-55 0.7488727155585835 1102 56-57 0.8245714820969283 1102 58-59 0.947050445876144 1102 60-61 0.9182426582605103 1102 62-63 0.6697866709445321 1102 64-65 0.6721023877522825 1102 66-67 0.7309649566387861 1102 68-69 0.9122356905248594 1102 70-71 0.8426849548072752 1102 72-73 0.6891993523569973 1102 74-75 0.9502984452314536 1102 76-77 1.0295446479298924 1102 78-79 1.0208962034933933 1102 80-81 0.9002794542587509 1102 82-83 0.9650434901806264 1102 84-85 1.1501131447370874 1102 86-87 1.1587305192772206 1102 88-89 1.1316228751518942 1102 90-91 1.0904373352497814 1102 92-93 1.0976870911962457 1102 94-95 1.076255465187998 1102 96-97 1.0209899246700118 1102 98-99 1.0761431585873389 1102 100-101 1.1886236005419022 1103 1 0.4636916871468877 1103 2 0.5491628063248974 1103 3 0.4636336522521347 1103 4 0.24773864195881856 1103 5 0.3384054641876375 1103 6 0.3838545792846446 1103 7 0.4521585851200669 1103 8 0.3715662620960103 1103 9 0.5102528160331374 1103 10-11 0.47285888119261443 1103 12-13 0.44434173062129645 1103 14-15 0.6280405717273396 1103 16-17 0.6709863065528623 1103 18-19 0.6260275639809905 1103 20-21 0.7353288673095122 1103 22-23 0.5859764841669985 1103 24-25 0.5404035741913731 1103 26-27 0.5997783100062222 1103 28-29 0.5399458502729217 1103 30-31 0.6505667703041738 1103 32-33 0.7265638076701109 1103 34-35 0.7501906276802686 1103 36-37 0.6637745952558376 1103 38-39 0.6633654089056762 1103 40-41 0.7823226949229749 1103 42-43 0.8545864222928756 1103 44-45 0.8121622041676346 1103 46-47 0.6266558188293772 1103 48-49 0.6727997439460509 1103 50-51 0.736099808854064 1103 52-53 0.7666038836125821 1103 54-55 0.8480527288363788 1103 56-57 0.9240664747570122 1103 58-59 0.9019015604998302 1103 60-61 0.8468024374420366 1103 62-63 0.7434997689012022 1103 64-65 0.7568269600493309 1103 66-67 0.6363619074847122 1103 68-69 0.6647637681839242 1103 70-71 0.5362088154557867 1103 72-73 0.6338970233661563 1103 74-75 0.6175418538717423 1103 76-77 0.7480586334626089 1103 78-79 0.9009179479542055 1103 80-81 0.8021608958912161 1103 82-83 0.7760493787585894 1103 84-85 0.8105183958019033 1103 86-87 0.8440811611117311 1103 88-89 0.8933033605786527 1103 90-91 0.8399054874411576 1103 92-93 0.9235651122294399 1103 94-95 0.9399911110589834 1103 96-97 0.8827100546054076 1103 98-99 0.9764353787886577 1103 100-101 1.332713303725047 1104 1 0.4452631211442153 1104 2 0.4550151511443268 1104 3 0.4346418114350996 1104 4 0.21009633179500042 1104 5 0.3882572908558757 1104 6 0.43124148228753967 1104 7 0.4275721874226548 1104 8 0.39790960303486145 1104 9 0.538609087901321 1104 10-11 0.4991572158522004 1104 12-13 0.4727952330362015 1104 14-15 0.6693176600264223 1104 16-17 0.6210016225833925 1104 18-19 0.6998109199641362 1104 20-21 0.6673129540772962 1104 22-23 0.6087579988297804 1104 24-25 0.6477652427987906 1104 26-27 0.7787337752507213 1104 28-29 0.7366759395104125 1104 30-31 0.6751329807939896 1104 32-33 0.8164843078990813 1104 34-35 0.786504403430726 1104 36-37 0.7616065509184011 1104 38-39 0.6683358622447386 1104 40-41 0.6936259331826733 1104 42-43 0.6290476438636503 1104 44-45 0.6734971243775902 1104 46-47 0.7531957206400293 1104 48-49 0.7753397192489544 1104 50-51 0.713384298254347 1104 52-53 0.7567692010172422 1104 54-55 0.7912625627778738 1104 56-57 0.8314669815446933 1104 58-59 0.7164467793816556 1104 60-61 0.7356412893326194 1104 62-63 0.7243754583155706 1104 64-65 0.7657306765644307 1104 66-67 0.6839329656834678 1104 68-69 0.6251128887627644 1104 70-71 0.6680733924106619 1104 72-73 0.746102270835852 1104 74-75 0.9397425404992674 1104 76-77 1.0205650539981939 1104 78-79 1.0462624073338347 1104 80-81 0.9195251907655617 1104 82-83 1.0655418484283707 1104 84-85 1.1128967885265375 1104 86-87 1.0807540530037159 1104 88-89 1.1323135682807042 1104 90-91 1.0994767713660885 1104 92-93 1.1547026949583952 1104 94-95 1.1989077967934882 1104 96-97 1.2166940490446976 1104 98-99 1.361365825173749 1104 100-101 1.6469090484624012 1105 1 0.2126290425536972 1105 2 0.2676081318687302 1105 3 0.2778383238943505 1105 4 0.1379514492467706 1105 5 0.17580783068988382 1105 6 0.21064169514255582 1105 7 0.30901291506394557 1105 8 0.24423212028136732 1105 9 0.38379720171744935 1105 10-11 0.32983272839569366 1105 12-13 0.3291209656019092 1105 14-15 0.40538990282404086 1105 16-17 0.38239251939282326 1105 18-19 0.3869314714994445 1105 20-21 0.38685254594788177 1105 22-23 0.5037693362482045 1105 24-25 0.4995009859014061 1105 26-27 0.525415579058027 1105 28-29 0.5076525894124302 1105 30-31 0.514855603277141 1105 32-33 0.44328377298288046 1105 34-35 0.5658875252542757 1105 36-37 0.5166917392351067 1105 38-39 0.4491456992690743 1105 40-41 0.4438872605670028 1105 42-43 0.36639223125528275 1105 44-45 0.3284349328707137 1105 46-47 0.310166854659748 1105 48-49 0.26123752326090965 1105 50-51 0.424141305246458 1105 52-53 0.27500367761929567 1105 54-55 0.306138706829465 1105 56-57 0.40444348060458424 1105 58-59 0.25878501332017123 1105 60-61 0.35649506023165145 1105 62-63 0.20341678156276544 1105 64-65 0.2808286732309071 1105 66-67 0.3144228806517262 1105 68-69 0.38492038420552177 1105 70-71 0.40763419039202375 1105 72-73 0.37493799448776244 1105 74-75 0.4769453054091599 1105 76-77 0.5057686313385297 1105 78-79 0.5452231623560664 1105 80-81 0.6394261201207243 1105 82-83 0.7069359517358293 1105 84-85 0.6502683687214095 1105 86-87 0.5911628219598768 1105 88-89 0.642433002613874 1105 90-91 0.4890298949230001 1105 92-93 0.49652586034091684 1105 94-95 0.34539282218304024 1105 96-97 0.36827032731050835 1105 98-99 0.6182929856234729 1105 100-101 1.0423642013753103 1106 1 0.04283275630387351 1106 2 0.05229931766818652 1106 3 0.0859636234945036 1106 4 -0.00826820681943019 1106 5 0.11984654118213456 1106 6 0.14637168615814034 1106 7 0.1539111730056817 1106 8 0.14962937214068717 1106 9 0.06491643611886388 1106 10-11 0.07233949541226536 1106 12-13 0.07548543438752375 1106 14-15 0.1438572704474126 1106 16-17 -0.06461377543955393 1106 18-19 0.08296526783161795 1106 20-21 0.050760020810166395 1106 22-23 0.1293650920443028 1106 24-25 0.10805756619711815 1106 26-27 0.11457206290761235 1106 28-29 0.04558735049297269 1106 30-31 0.12352434525790557 1106 32-33 0.13339510224595585 1106 34-35 0.1608122628260773 1106 36-37 0.08160056032466656 1106 38-39 0.0720701698917594 1106 40-41 0.027152349650506835 1106 42-43 0.14891854583615327 1106 44-45 -0.1144955799873344 1106 46-47 -0.08393823430894543 1106 48-49 0.08152515375741132 1106 50-51 0.11514712932986271 1106 52-53 0.09915532481015532 1106 54-55 0.03831296432341702 1106 56-57 0.11594123737087614 1106 58-59 0.1660134500029926 1106 60-61 0.02172436378149456 1106 62-63 -0.08317067584737003 1106 64-65 -0.04364826437475955 1106 66-67 -0.018675652276300525 1106 68-69 0.025495429916979617 1106 70-71 -0.07531443482002942 1106 72-73 0.0038089719520897347 1106 74-75 0.20094381566086383 1106 76-77 0.13022030924289396 1106 78-79 0.18941881309476827 1106 80-81 0.03667216683394514 1106 82-83 0.18117737133104228 1106 84-85 0.20254719563791568 1106 86-87 0.2484677553517649 1106 88-89 0.28575116577431814 1106 90-91 0.23442782133115614 1106 92-93 0.36261837112652273 1106 94-95 0.26647993657791247 1106 96-97 0.3149892930405578 1106 98-99 0.3834305074745501 1106 100-101 0.608637979221978 1107 1 0.16597126210491808 1107 2 0.22262486415472438 1107 3 0.24894013786420643 1107 4 0.0645669559538149 1107 5 0.13288560050602172 1107 6 0.17283289265030533 1107 7 0.16879687210190752 1107 8 0.08335048418997104 1107 9 0.20360987202592185 1107 10-11 0.26955155407942755 1107 12-13 0.19870993950057425 1107 14-15 0.22084229093088936 1107 16-17 0.27828893588420556 1107 18-19 0.2808137214076254 1107 20-21 0.32084476922309335 1107 22-23 0.3990988069018542 1107 24-25 0.23509105902007832 1107 26-27 0.3376550600618131 1107 28-29 0.4566640618792803 1107 30-31 0.357305656825595 1107 32-33 0.44956062746720704 1107 34-35 0.237161670212636 1107 36-37 0.24807177400949598 1107 38-39 0.3043433662516435 1107 40-41 0.19961771538212503 1107 42-43 0.31452663915322177 1107 44-45 0.3020780510599792 1107 46-47 0.4138672196110562 1107 48-49 0.3670233243175005 1107 50-51 0.3279922992957722 1107 52-53 0.262923814662237 1107 54-55 0.450532684747472 1107 56-57 0.31321271374451953 1107 58-59 0.4375508049768797 1107 60-61 0.3732504606636269 1107 62-63 0.2882095349291305 1107 64-65 0.3782393423425603 1107 66-67 0.44225407846766984 1107 68-69 0.4335082203601033 1107 70-71 0.41400992331237774 1107 72-73 0.3330565561532346 1107 74-75 0.44592380127552644 1107 76-77 0.4569567135334758 1107 78-79 0.5528464329465379 1107 80-81 0.5217684860633938 1107 82-83 0.6457956907481233 1107 84-85 0.6180442716613079 1107 86-87 0.49285903658515196 1107 88-89 0.6695340409658641 1107 90-91 0.6762664866218344 1107 92-93 0.6291622933206042 1107 94-95 0.6120095359774815 1107 96-97 0.7425059456346119 1107 98-99 0.8362833391973474 1107 100-101 1.1756728793064148 1108 1 0.32307940041606287 1108 2 0.3737006054560297 1108 3 0.3413774773477307 1108 4 0.18335883360713012 1108 5 0.3104321260654359 1108 6 0.2825552504929618 1108 7 0.3504391348397675 1108 8 0.3273362990672979 1108 9 0.3671022455075601 1108 10-11 0.3202503480650094 1108 12-13 0.43225879308165105 1108 14-15 0.4788497089812722 1108 16-17 0.5904352571604932 1108 18-19 0.58805639519165 1108 20-21 0.4934020126718508 1108 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-0.9140635659129117 2316 88-89 -1.2349601602268443 2316 90-91 -0.8106705353064712 2316 92-93 -1.05197294950883 2316 94-95 -0.7724412373422567 2316 96-97 -0.7732214905831185 2316 98-99 -0.6161631234404688 2316 100-101 -0.7840277108891947 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 16.0 4 62.0 5 123.0 6 284.0 7 762.0 8 1778.0 9 3068.0 10 5060.0 11 8070.0 12 10961.0 13 14217.0 14 16347.0 15 20089.0 16 23002.0 17 26137.0 18 23589.0 19 20526.0 20 20561.0 21 21866.0 22 24405.0 23 28219.0 24 34439.0 25 43211.0 26 54773.0 27 69856.0 28 87862.0 29 108331.0 30 131788.0 31 159405.0 32 188890.0 33 227425.0 34 277935.0 35 343640.0 36 440372.0 37 554504.0 38 468090.0 39 79075.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.19974918295088 21.545732845642938 14.768698009659028 23.48581996174715 2 15.208923252567386 22.22374824083965 39.85627165392159 22.71105685267138 3 17.880633752566336 27.077619570423938 31.404861520288364 23.636885156721362 4 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21.44730283139687 24-25 21.534760519615133 27.400967775344547 29.693750689688642 21.370521015351674 26-27 21.474013117039846 27.503070001111997 29.600575581936706 21.422341299911448 28-29 21.492527654903988 27.658464567346336 29.540966248378485 21.308041529371188 30-31 21.74426706506259 27.404551644209796 29.45472878730352 21.396452503424097 32-33 21.609166032947375 27.542389708464626 29.35048589228501 21.49795836630299 34-35 21.83534546178058 27.561072528476306 29.335558134953775 21.26802387478934 36-37 21.871200512615093 27.41267139860698 29.26459939229396 21.451528696483965 38-39 21.697063499116982 27.507203525796132 29.300748279355933 21.494984695730952 40-41 21.75478789046836 27.49743052963164 29.293813178804335 21.453968401095665 42-43 21.760142412316323 27.547861243513417 29.009698574915966 21.68229776925429 44-45 21.699497055855723 27.641947517065663 29.041594171709228 21.616961255369386 46-47 21.9360021365121 27.706732627238694 28.66194570970142 21.695319526547788 48-49 21.824795208361035 27.848582422602675 28.48166275648677 21.844959612549523 50-51 21.886403740256917 27.908470508554434 28.426062989985663 21.77906276120299 52-53 22.035182070231542 27.887895407422192 28.344723163030373 21.732199359315896 54-55 21.815802277850377 27.819406172747563 28.50108242620049 21.86370912320157 56-57 21.82806333466021 27.99316127429556 28.281965877873155 21.896809513171075 58-59 21.836301473143443 27.906543907775095 28.434010123948344 21.82314449513312 60-61 21.85590903089162 28.033922285062214 28.378536940649713 21.731631743396456 62-63 21.816568760338434 27.977016150018443 28.404045764042728 21.80236932560039 64-65 21.644591889354466 27.88398953264345 28.340656390719452 22.13076218728263 66-67 21.594377302138117 27.918017079916456 28.343204539481853 22.14440107846357 68-69 21.723705565986698 27.926437464419195 28.337601261637797 22.01225570795631 70-71 21.715247226560756 27.722003834250415 28.302812443607728 22.25993649558111 72-73 21.69824579461822 27.888299698385488 28.165934357416305 22.247520149579987 74-75 21.664646049143855 28.000007932322557 28.083268989449017 22.252077029084568 76-77 21.621134778188118 27.870371236465648 28.126949600152155 22.38154438519408 78-79 21.512493139752358 27.94286960979533 28.154993271163896 22.389643979288408 80-81 21.6121102477109 28.010592524645904 28.005025543119803 22.372271684523394 82-83 21.69101471112265 27.90730401771698 28.07110474853981 22.33057652262055 84-85 21.60831107146748 27.917757388591646 27.91261432887521 22.56131721106566 86-87 21.494377536028956 27.949861405902816 27.98016870924233 22.5755923488259 88-89 21.475245634780407 27.838825095772098 27.935102315386594 22.7508269540609 90-91 21.459341843167515 27.84604130496433 27.97698538109078 22.717631470777373 92-93 21.48937538288261 27.63807974698481 28.07769081244246 22.79485405769012 94-95 21.274656695379708 27.603177895573904 28.111345598121794 23.010819810924595 96-97 21.286380420662546 27.45420630047155 28.137116739776264 23.12229653908964 98-99 21.168259648672652 27.50641398617761 28.04188539967915 23.283440965470586 100-101 21.09792409807867 27.462556704328733 28.00124959343 23.4382696041626 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 455.0 1 357.0 2 404.0 3 568.0 4 482.5 5 444.5 6 658.5 7 1243.5 8 1973.5 9 2340.0 10 2165.0 11 1904.5 12 1998.5 13 2253.5 14 2573.0 15 2861.0 16 3216.0 17 3644.5 18 4017.5 19 4555.5 20 5238.0 21 6173.5 22 7617.5 23 9564.5 24 12233.5 25 16189.5 26 21232.5 27 27359.5 28 35185.5 29 45189.5 30 56386.0 31 67615.5 32 79133.0 33 86608.5 34 91021.0 35 95496.5 36 102766.5 37 111651.5 38 117163.5 39 119760.0 40 120622.0 41 121666.5 42 123040.0 43 124774.5 44 125820.0 45 127801.5 46 129044.5 47 128971.0 48 128389.0 49 125168.0 50 120230.0 51 114885.5 52 111143.5 53 107945.0 54 103239.5 55 95158.0 56 84248.5 57 73054.0 58 63833.5 59 55224.0 60 45681.5 61 37445.5 62 30705.0 63 23467.0 64 17092.0 65 12643.5 66 9099.0 67 7072.5 68 5406.5 69 3919.5 70 2923.5 71 2125.0 72 1570.5 73 1188.0 74 857.5 75 642.5 76 470.5 77 281.5 78 171.5 79 104.5 80 60.0 81 36.5 82 21.0 83 11.0 84 8.0 85 6.5 86 4.5 87 3.0 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020176610335115412 2 0.0037018710838937242 3 0.004125749452278502 4 0.03439066495495162 5 0.0019215819366776585 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 5.651711578463701E-5 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.47756736769555E-5 24-25 4.6626620522325534E-4 26-27 0.002020486889300773 28-29 7.064639473079627E-5 30-31 0.006428821920502459 32-33 0.021914511645493 34-35 0.012942419514681875 36-37 9.466616893926699E-4 38-39 9.184031315003513E-4 40-41 0.002373718862954754 42-43 0.00827975746244932 44-45 0.008096076836149251 46-47 0.0077287155835491115 48-49 0.009537263288657496 50-51 0.1982337836146143 52-53 0.049466605590503546 54-55 0.01793005498267609 56-57 0.010526312814888642 58-59 0.019908154035138386 60-61 0.05374777711118979 62-63 0.0960508382759906 64-65 0.10088305167557707 66-67 0.10517835247520949 68-69 0.10352522683850884 70-71 0.09181205459214282 72-73 0.06920520827828801 74-75 0.2512044503837654 76-77 0.3047402883107628 78-79 0.007036380915187307 80-81 8.194981788772366E-4 82-83 0.0 84-85 4.238783683847775E-5 86-87 0.0 88-89 3.249734157616628E-4 90-91 0.006146236341579274 92-93 0.017619210845860586 94-95 0.0016107377998621547 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3538751.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.361337125581954 #Duplication Level Percentage of deduplicated Percentage of total 1 78.39715166650866 45.753625980944925 2 12.182801615527136 14.220091844357274 3 3.748027855669562 6.562197517224099 4 1.7522381047705955 4.090518350272301 5 0.9759342216843782 2.847841306205722 6 0.6096157902472699 2.1346795591019383 7 0.4224353493901984 1.7257724287667058 8 0.3082323305725686 1.4391080766039601 9 0.23956146583318316 1.2583014722810089 >10 1.2660081224226472 13.213431855278113 >50 0.07107907752099653 2.807432474514954 >100 0.024780307217118727 2.5113795375136507 >500 0.001455063167847795 0.5535331632246089 >1k 5.82025258060774E-4 0.48613543458527614 >5k 9.700420967679548E-5 0.39595099912535425 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7683 0.21711050028668308 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6305 0.17817020751106818 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4129 0.1166795855373831 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.6517115784637006E-5 2 0.0 0.0 0.0 0.0 5.6517115784637006E-5 3 0.0 0.0 0.0 0.0 5.6517115784637006E-5 4 0.0 0.0 0.0 0.0 5.6517115784637006E-5 5 0.0 0.0 0.0 0.0 5.6517115784637006E-5 6 0.0 0.0 0.0 2.8258557892318503E-5 1.1303423156927401E-4 7 0.0 0.0 0.0 2.8258557892318503E-5 1.1303423156927401E-4 8 0.0 0.0 0.0 2.8258557892318503E-5 1.1303423156927401E-4 9 0.0 0.0 0.0 2.8258557892318503E-5 1.1303423156927401E-4 10-11 0.0 0.0 0.0 2.8258557892318503E-5 1.1303423156927401E-4 12-13 2.8258557892318503E-5 0.0 0.0 4.238783683847776E-5 2.119391841923888E-4 14-15 2.8258557892318503E-5 0.0 0.0 5.6517115784637006E-5 2.967148578693443E-4 16-17 2.8258557892318503E-5 0.0 0.0 8.477567367695552E-5 3.3910269470782206E-4 18-19 2.8258557892318503E-5 0.0 0.0 9.890495262311476E-5 3.6736125260014053E-4 20-21 2.8258557892318503E-5 0.0 0.0 1.1303423156927401E-4 4.803954841694146E-4 22-23 2.8258557892318503E-5 0.0 0.0 1.6955134735391103E-4 5.510418789002108E-4 24-25 4.238783683847776E-5 0.0 0.0 3.3910269470782206E-4 6.075589946848478E-4 26-27 5.6517115784637006E-5 0.0 0.0 8.336274578233959E-4 6.782053894156441E-4 28-29 5.6517115784637006E-5 0.0 0.0 0.002303072468223958 6.782053894156441E-4 30-31 7.064639473079625E-5 0.0 0.0 0.006838571009941079 6.923346683618034E-4 32-33 8.477567367695552E-5 0.0 0.0 0.020077705382492296 7.347225052002811E-4 34-35 9.890495262311476E-5 0.0 0.0 0.038459897291445486 7.629810630925996E-4 36-37 1.1303423156927401E-4 0.0 0.0 0.06759447047842586 7.912396209849181E-4 38-39 1.1303423156927401E-4 0.0 0.0 0.11227125050618142 8.901445736080329E-4 40-41 1.1303423156927401E-4 0.0 0.0 0.16149765835460025 9.042738525541921E-4 42-43 1.1303423156927401E-4 0.0 0.0 0.21068167836618062 9.184031315003513E-4 44-45 1.1303423156927401E-4 0.0 0.0 0.27051917470316506 9.466616893926699E-4 46-47 1.1303423156927401E-4 0.0 0.0 0.3453760945599168 9.607909683388291E-4 48-49 1.1303423156927401E-4 0.0 0.0 0.4216318130323382 0.0010173080841234662 50-51 1.1303423156927401E-4 0.0 0.0 0.5024936764412076 0.0010173080841234662 52-53 1.1303423156927401E-4 0.0 0.0 0.5867041789603168 0.0010738251999081031 54-55 1.1303423156927401E-4 0.0 0.0 0.680861693857522 0.00113034231569274 56-57 1.1303423156927401E-4 0.0 0.0 0.7849238332959849 0.001172730152531218 58-59 1.1303423156927401E-4 0.0 0.0 0.9012925746965526 0.0011868594314773772 60-61 1.1303423156927401E-4 0.0 0.0 1.0272409672226162 0.0011868594314773772 62-63 1.1303423156927401E-4 0.0 0.0 1.1613419537006135 0.0012151179893696957 64-65 1.1303423156927401E-4 0.0 0.0 1.3077071543038772 0.0012433765472620142 66-67 1.1303423156927401E-4 0.0 0.0 1.4678060140428077 0.0012433765472620142 68-69 1.1303423156927401E-4 0.0 0.0 1.6475304422379535 0.0012433765472620142 70-71 1.1303423156927401E-4 0.0 0.0 1.8453544767631291 0.0012575058262081734 72-73 1.1303423156927401E-4 0.0 0.0 2.0528994552032627 0.0014129278946159252 74-75 1.1303423156927401E-4 0.0 0.0 2.2769333021735636 0.0014694450104005621 76-77 1.1303423156927401E-4 0.0 0.0 2.525990102157513 0.0014694450104005621 78-79 1.1303423156927401E-4 0.0 0.0 2.79812001466054 0.0014694450104005621 80-81 1.1303423156927401E-4 0.0 0.0 3.0962901882613387 0.00151183284723904 82-83 1.1303423156927401E-4 0.0 0.0 3.413520759160506 0.001681384194592951 84-85 1.1303423156927401E-4 0.0 0.0 3.747381491379303 0.0016955134735391103 86-87 1.1303423156927401E-4 0.0 0.0 4.105714134732848 0.0017520305893237473 88-89 1.1303423156927401E-4 0.0 0.0 4.495286613836351 0.0018368062630007029 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4385 0.0 58.474773 1 GTATCAA 8540 0.0 42.368343 1 ATCAACG 11070 0.0 31.779078 3 TCAACGC 11105 0.0 31.591629 4 CAACGCA 11235 0.0 31.216377 5 TATCAAC 11475 0.0 30.947075 2 AACGCAG 11990 0.0 29.488195 6 TAACGCA 415 0.0 26.310305 4 ACGCAGA 13595 0.0 25.93706 7 CGCAGAG 14150 0.0 24.785582 8 GTACATG 9370 0.0 24.324615 1 TACATGG 9760 0.0 22.521738 2 TATAACG 430 0.0 22.081686 2 ACATGGG 9775 0.0 21.952923 3 GCAGAGT 15725 0.0 21.639122 9 GTGGTAT 2220 0.0 20.961264 1 AGAGTAC 14915 0.0 20.348314 10-11 TGGTATC 2100 0.0 20.346695 2 ATAACGC 495 0.0 19.18207 3 GAGTACT 10930 0.0 18.952879 12-13 >>END_MODULE