FastQCFastQC Report
Sat 18 Jun 2016
SRR1930918_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930918_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232339
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN58900.18222098610325219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27650.055.6230851
GTATCAA50000.042.8616871
ATCAACG59200.035.677043
TCAACGC60500.035.2341544
TATCAAC61750.034.534582
CAACGCA62400.033.252065
AACGCAG67350.031.308616
GTACATG61850.026.4153041
ACGCAGA79700.026.3015887
CGCAGAG82850.025.2880788
TACATGG61950.025.2273242
ACATGGG65000.023.6528223
GCAGAGT88800.022.5790129
TATACCG2404.818503E-922.168045
GTGGTAT13900.021.0408631
CATGGGG48600.020.9456544
CAGAGTA85600.019.13201310-11
GAGTACT57350.018.83449212-13
AGAGTAC80900.018.13993610-11
TGGTATC15750.017.6048742
Produced by FastQC (version 0.11.5)