FastQCFastQC Report
Sat 18 Jun 2016
SRR1930918_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930918_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232339
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56720.17547664400299598No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50420.15598611408023724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37850.055.5708581
GTATCAA68900.040.4508971
TCAACGC85900.032.106884
ATCAACG86800.031.7754523
CAACGCA87400.031.5998945
TATCAAC90000.030.6993542
AACGCAG92250.030.0919346
ACGCAGA105700.026.2179347
CGCAGAG109150.025.3022928
GTACATG86100.024.5946671
TACATGG87950.023.318292
ACATGGG88100.022.3079553
GCAGAGT125700.021.253669
GTGGTAT20000.021.1285291
AGAGTAC114300.019.6162610-11
GAGTACT81000.019.09823412-13
CAGAGTA120400.018.95741810-11
CATGGGG63750.018.8388984
TGGTATC20650.018.3915652
AGTACTT89400.017.861112-13
Produced by FastQC (version 0.11.5)