FastQCFastQC Report
Sat 18 Jun 2016
SRR1930917_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930917_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628622
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48030.18271931072630448No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22050.053.0366331
GTATCAA42350.046.813631
ATCAACG51650.037.392993
TATCAAC52250.037.3560642
TCAACGC53050.036.391584
CAACGCA53700.034.6734855
AACGCAG60250.031.4469436
GTACATG58250.028.4345681
ACGCAGA68250.027.509547
TACATGG60100.026.5491432
CGCAGAG71250.026.155588
ACATGGG61900.025.6902543
GTACTAG4150.025.3980181
GCAGAGT76550.023.148739
CATGGGG46450.022.8863054
GTGGTAT10100.022.8594151
GAGTACA43900.020.8082471
CAGAGTA73100.019.60008810-11
GAGTACT48900.019.42066212-13
GTACTTT51350.018.98143414-15
Produced by FastQC (version 0.11.5)