##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930917_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2628622 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 23.375088924919595 30.0 16.0 31.0 2.0 33.0 2 22.275669533314414 30.0 10.0 31.0 2.0 33.0 3 22.740067229141353 30.0 16.0 31.0 2.0 34.0 4 24.761681595908428 33.0 10.0 35.0 2.0 37.0 5 24.749240856996554 33.0 10.0 35.0 2.0 37.0 6 25.49916990727461 33.0 17.0 35.0 2.0 37.0 7 25.579162770455394 33.0 17.0 35.0 2.0 37.0 8 26.348743181788784 33.0 17.0 35.0 2.0 37.0 9 27.518642467422094 34.0 17.0 37.0 2.0 39.0 10-11 27.76526959753057 34.0 21.0 37.0 2.0 39.0 12-13 27.70777654603819 34.0 22.0 37.0 2.0 39.0 14-15 27.985626879787205 34.0 18.5 38.0 2.0 40.0 16-17 27.11754485810436 33.5 17.0 38.0 2.0 40.0 18-19 26.4997987538718 33.0 11.5 38.0 2.0 40.0 20-21 25.984561682889364 33.0 10.0 38.0 2.0 40.0 22-23 25.901799307774187 32.0 10.0 38.0 2.0 40.0 24-25 25.59723611839207 32.0 10.0 38.0 2.0 40.0 26-27 25.253188552785453 32.0 10.0 38.0 2.0 40.0 28-29 25.17320481986379 32.0 9.0 38.0 2.0 40.0 30-31 25.064640142249438 32.0 8.5 38.0 2.0 40.0 32-33 24.742383842180427 31.5 8.0 38.0 2.0 40.0 34-35 21.765656682474695 27.0 2.0 36.0 2.0 39.0 36-37 20.114294295642356 20.0 2.0 35.0 2.0 38.5 38-39 19.14294657048446 19.5 2.0 34.0 2.0 38.0 40-41 20.475116810252672 24.5 2.0 35.0 2.0 38.0 42-43 20.389176724534757 24.5 2.0 35.0 2.0 38.0 44-45 18.376116649712284 19.0 2.0 34.0 2.0 38.0 46-47 19.21051258035579 19.0 2.0 33.0 2.0 38.0 48-49 18.618068326294157 19.5 2.0 34.0 2.0 38.0 50-51 16.56880715447105 11.0 2.0 32.0 2.0 37.5 52-53 19.500972753024207 23.0 2.0 33.5 2.0 38.0 54-55 21.166220171633654 27.5 2.0 35.5 2.0 38.5 56-57 21.37693571004123 28.0 2.0 36.0 2.0 39.0 58-59 20.992706444669487 27.5 2.0 36.0 2.0 39.0 60-61 20.665377144374503 27.0 2.0 35.0 2.0 39.0 62-63 20.176257179617306 26.0 2.0 35.0 2.0 38.5 64-65 19.656058573655702 25.5 2.0 34.0 2.0 38.0 66-67 19.21721704375905 25.0 2.0 34.0 2.0 38.0 68-69 18.745583807789785 23.0 2.0 34.0 2.0 37.0 70-71 18.144436704859046 21.5 2.0 33.5 2.0 36.5 72-73 17.664952016684026 20.0 2.0 33.0 2.0 36.0 74-75 17.121816868305906 18.0 2.0 32.0 2.0 35.5 76-77 16.558368224872197 13.0 2.0 32.0 2.0 35.0 78-79 16.058684169880646 7.5 2.0 31.0 2.0 35.0 80-81 15.54582952588847 7.0 2.0 31.0 2.0 34.0 82-83 15.059549261932679 2.0 2.0 31.0 2.0 34.0 84-85 14.534647050812175 2.0 2.0 30.0 2.0 34.0 86-87 13.970420433215578 2.0 2.0 30.0 2.0 34.0 88-89 13.413256831906605 2.0 2.0 29.0 2.0 34.0 90-91 12.83783442427249 2.0 2.0 29.0 2.0 34.0 92-93 12.207554186185765 2.0 2.0 29.0 2.0 34.0 94-95 11.540520280207652 2.0 2.0 27.0 2.0 34.0 96-97 10.799567986572432 2.0 2.0 25.5 2.0 33.0 98-99 9.986821041595178 2.0 2.0 24.5 2.0 32.5 100-101 8.911828136567372 2.0 2.0 10.5 2.0 31.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 6.9374856346521945 1101 2 8.082148529882016 1101 3 7.66877139993953 1101 4 9.401215662439316 1101 5 9.289238387507094 1101 6 8.66467586148028 1101 7 8.435085507336321 1101 8 7.611835190269922 1101 9 7.695776528194198 1101 10-11 7.039119185521766 1101 12-13 6.850466756328874 1101 14-15 7.354786959058213 1101 16-17 8.143209929263435 1101 18-19 8.775206461039367 1101 20-21 9.202878387578764 1101 22-23 9.059613286238989 1101 24-25 8.960329977586358 1101 26-27 9.02274565727193 1101 28-29 9.028148150580147 1101 30-31 8.817051223065562 1101 32-33 8.73046271917076 1101 34-35 11.362810596985785 1101 36-37 5.336564055560483 1101 38-39 8.65370024214554 1101 40-41 10.06616216039956 1101 42-43 10.358463807605204 1101 44-45 4.66620389799834 1101 46-47 6.08627065432653 1101 48-49 9.332111314234592 1101 50-51 -3.8035186848818032 1101 52-53 5.983762903677924 1101 54-55 8.553943512719837 1101 56-57 9.270354351941162 1101 58-59 9.461110634022155 1101 60-61 9.159420223844332 1101 62-63 9.118801840997733 1101 64-65 9.057453845322353 1101 66-67 9.189406419579132 1101 68-69 8.96818924557095 1101 70-71 9.130086312553988 1101 72-73 8.866303777548293 1101 74-75 8.84119845502746 1101 76-77 8.695605197910648 1101 78-79 8.593528593025866 1101 80-81 8.382233151891612 1101 82-83 8.261673884848236 1101 84-85 8.203331512236447 1101 86-87 7.989686626545607 1101 88-89 7.960258214450086 1101 90-91 7.816523127115593 1101 92-93 7.657512946417915 1101 94-95 7.487162509176317 1101 96-97 7.237238935182848 1101 98-99 6.871745360921821 1101 100-101 6.30130949494025 1102 1 6.4994555950401995 1102 2 7.670314832288604 1102 3 7.437380077500354 1102 4 9.115915565005754 1102 5 9.047403598163402 1102 6 8.39031798573794 1102 7 8.060631382477165 1102 8 7.299004961558538 1102 9 7.1793539260794965 1102 10-11 6.851776897089575 1102 12-13 6.381111208280533 1102 14-15 6.91657384528342 1102 16-17 7.465747980880565 1102 18-19 7.928381471699382 1102 20-21 8.357809210779081 1102 22-23 8.100120955269734 1102 24-25 8.31324971437154 1102 26-27 8.398705831045614 1102 28-29 8.068905197004252 1102 30-31 8.063086289878626 1102 32-33 8.008517869039238 1102 34-35 10.434232951911742 1102 36-37 0.9127836745190336 1102 38-39 3.222052178148374 1102 40-41 8.114521805601317 1102 42-43 9.15155009295577 1102 44-45 0.8018209119995241 1102 46-47 1.5502440537279192 1102 48-49 7.377043790509621 1102 50-51 -4.935723721764424 1102 52-53 4.486952797160175 1102 54-55 7.389205476140024 1102 56-57 7.955560517652376 1102 58-59 8.320554479949411 1102 60-61 8.09813569724167 1102 62-63 8.089620823651263 1102 64-65 8.083794895750913 1102 66-67 8.090916124827157 1102 68-69 8.00522378401807 1102 70-71 8.021083930272479 1102 72-73 7.919468926407415 1102 74-75 7.867162800111188 1102 76-77 7.7040375526512115 1102 78-79 7.695362887992069 1102 80-81 7.483182459660135 1102 82-83 7.3930915225890175 1102 84-85 7.29918165097113 1102 86-87 7.0607002188366135 1102 88-89 6.949002243297009 1102 90-91 6.803497696543699 1102 92-93 6.797116522621058 1102 94-95 6.635988671632875 1102 96-97 6.633451076681926 1102 98-99 6.2080403672972295 1102 100-101 5.6535374894773796 1103 1 6.5453265778258185 1103 2 7.7391894013182 1103 3 7.3923880910551745 1103 4 9.011881906039026 1103 5 9.16797017787231 1103 6 8.377887302478026 1103 7 8.1443524109818 1103 8 7.239073855728698 1103 9 7.442853253022353 1103 10-11 6.842747137984976 1103 12-13 6.441685587713696 1103 14-15 7.036033851840887 1103 16-17 7.623054978434752 1103 18-19 8.105599785574135 1103 20-21 8.6313126259586 1103 22-23 8.746966419923549 1103 24-25 8.610552470952943 1103 26-27 8.600136764792762 1103 28-29 8.608546073668823 1103 30-31 8.256026241165983 1103 32-33 8.111328366286926 1103 34-35 10.630371324722269 1103 36-37 -3.054489049051 1103 38-39 -1.5572508818687751 1103 40-41 6.9161850509790135 1103 42-43 9.665802154384195 1103 44-45 1.266863688294066 1103 46-47 0.715525785674 1103 48-49 7.7565408508485305 1103 50-51 -5.146614875899601 1103 52-53 4.570401064419865 1103 54-55 7.605245746544039 1103 56-57 8.286419016806217 1103 58-59 8.40816646097755 1103 60-61 8.445759785152507 1103 62-63 8.3805947047574 1103 64-65 8.23436979448687 1103 66-67 8.127696508622467 1103 68-69 8.082397269843142 1103 70-71 8.0309495377651 1103 72-73 7.965023999995676 1103 74-75 7.643286530619513 1103 76-77 7.490252685541815 1103 78-79 7.6351520205120345 1103 80-81 7.421188907160662 1103 82-83 7.201452755719313 1103 84-85 7.084555207869405 1103 86-87 6.898095950982272 1103 88-89 6.86211843169599 1103 90-91 6.694961504193946 1103 92-93 6.567560633421943 1103 94-95 6.384736235491845 1103 96-97 6.154196204908981 1103 98-99 5.885140565243235 1103 100-101 5.479293075037097 1104 1 6.400231559276087 1104 2 7.445824140859088 1104 3 7.137612604240928 1104 4 8.908430204189262 1104 5 8.805265377267997 1104 6 8.023991858233284 1104 7 7.774404841424943 1104 8 6.851485246791334 1104 9 6.957357396478596 1104 10-11 6.338562377419816 1104 12-13 6.061982383465679 1104 14-15 6.559437774635295 1104 16-17 7.013634987886363 1104 18-19 7.752315161573719 1104 20-21 7.954852183220908 1104 22-23 7.874415561649474 1104 24-25 7.957736892897078 1104 26-27 8.1112450230527 1104 28-29 8.040412545349316 1104 30-31 7.5474764322449985 1104 32-33 7.607614140110407 1104 34-35 9.932378863691593 1104 36-37 -3.281696690722967 1104 38-39 -2.2254409710637866 1104 40-41 5.801595694098491 1104 42-43 8.120789930537093 1104 44-45 0.3162508214391089 1104 46-47 -0.24076810016977745 1104 48-49 6.837904414481375 1104 50-51 -5.425287146558274 1104 52-53 3.785114148215545 1104 54-55 6.598437975377436 1104 56-57 6.964645312403771 1104 58-59 7.352736430873765 1104 60-61 7.4388327524030124 1104 62-63 7.329664241144275 1104 64-65 7.212573205256508 1104 66-67 7.029572320289635 1104 68-69 7.3007280170048645 1104 70-71 7.147755560009898 1104 72-73 7.040467091196366 1104 74-75 6.945766464532596 1104 76-77 6.767196128387422 1104 78-79 6.608386260679929 1104 80-81 6.444679531823432 1104 82-83 6.392309057605544 1104 84-85 6.219751328984097 1104 86-87 5.956210390006296 1104 88-89 5.848655640937743 1104 90-91 5.653121340762786 1104 92-93 5.483958821985484 1104 94-95 5.247280470189709 1104 96-97 4.9293023495783075 1104 98-99 4.636253549313162 1104 100-101 4.25104445277816 1105 1 5.7554816018570065 1105 2 6.921942538039122 1105 3 6.581075136732025 1105 4 8.378596775820245 1105 5 8.257154735986969 1105 6 7.51261324842017 1105 7 7.4039719230396095 1105 8 6.375363764037225 1105 9 6.360232410980796 1105 10-11 5.876469086938936 1105 12-13 5.730513724258689 1105 14-15 5.907665791154226 1105 16-17 6.720020645150669 1105 18-19 7.422433227980207 1105 20-21 7.581240321286771 1105 22-23 7.574693942142719 1105 24-25 7.6100537270487045 1105 26-27 7.553131691151069 1105 28-29 7.3936894867308105 1105 30-31 6.980303546295218 1105 32-33 6.879196064570621 1105 34-35 9.207114553416137 1105 36-37 -3.7054430836868697 1105 38-39 -2.551053087249226 1105 40-41 5.2024042379296915 1105 42-43 7.623161749618774 1105 44-45 -3.4616050007923285 1105 46-47 -4.5493010701459085 1105 48-49 4.450137049487584 1105 50-51 -9.858623256908277 1105 52-53 0.17908413421938363 1105 54-55 4.971495297938414 1105 56-57 5.8954037958811405 1105 58-59 6.085477395695754 1105 60-61 6.078554088036952 1105 62-63 6.280019082163154 1105 64-65 6.250276822289866 1105 66-67 5.9020900993674665 1105 68-69 5.794795322015094 1105 70-71 5.854541971635353 1105 72-73 5.710437667161965 1105 74-75 5.516858029560453 1105 76-77 5.5075565481242315 1105 78-79 5.267778316190643 1105 80-81 5.233757957242641 1105 82-83 4.898704650418374 1105 84-85 4.839372000849343 1105 86-87 4.789170985991104 1105 88-89 4.802421337253072 1105 90-91 4.763521638458974 1105 92-93 4.660255750481623 1105 94-95 4.271004153328407 1105 96-97 3.8436150934929376 1105 98-99 3.640982993835774 1105 100-101 3.323740392903211 1106 1 5.704059978491664 1106 2 7.073618758292724 1106 3 6.701507157377698 1106 4 8.102972318637686 1106 5 8.040193272923364 1106 6 7.2362376559737065 1106 7 6.907844723853529 1106 8 6.2159511235250555 1106 9 6.079357947594605 1106 10-11 5.715432957010147 1106 12-13 5.551660899126556 1106 14-15 5.767790856292319 1106 16-17 6.368290081116825 1106 18-19 6.654125577666306 1106 20-21 7.083950190018008 1106 22-23 6.838828163835569 1106 24-25 7.243590663449108 1106 26-27 7.131527929271126 1106 28-29 7.116287442015061 1106 30-31 6.658638368499247 1106 32-33 6.5890745975366976 1106 34-35 9.102002267751484 1106 36-37 -0.16028675226425548 1106 38-39 0.8014204232597208 1106 40-41 6.565017205993417 1106 42-43 7.537277484500535 1106 44-45 -3.4582977226960967 1106 46-47 -4.48919833806819 1106 48-49 4.4174341288195755 1106 50-51 -9.740603579000638 1106 52-53 0.014381163922351448 1106 54-55 4.6129433487585345 1106 56-57 5.756407046582151 1106 58-59 6.271341188841987 1106 60-61 6.117552326206631 1106 62-63 6.090409166284349 1106 64-65 5.99994530041587 1106 66-67 5.791267002020138 1106 68-69 5.521916590532925 1106 70-71 5.582936969029827 1106 72-73 5.6207150934008325 1106 74-75 5.5828997427543285 1106 76-77 5.429216887090462 1106 78-79 5.290926905485662 1106 80-81 5.084121986126732 1106 82-83 5.054706412248695 1106 84-85 4.827146387310544 1106 86-87 4.482295629282499 1106 88-89 4.633029665003143 1106 90-91 4.218243964074992 1106 92-93 4.034480545141541 1106 94-95 3.9441639091536143 1106 96-97 3.7893179645319215 1106 98-99 3.768469035673041 1106 100-101 3.520558552162747 1107 1 5.9152817499212915 1107 2 7.034892587003892 1107 3 6.544356413569918 1107 4 7.99752279196991 1107 5 8.387831799016848 1107 6 6.8549108436948565 1107 7 7.138884202169169 1107 8 6.315041881041871 1107 9 6.308051698868251 1107 10-11 5.794961477360655 1107 12-13 5.681635333628865 1107 14-15 6.042327620589543 1107 16-17 6.829695257203824 1107 18-19 7.074791577406948 1107 20-21 7.6384449009241 1107 22-23 7.52753821774542 1107 24-25 7.567871772769397 1107 26-27 7.548165140038275 1107 28-29 7.376734097195779 1107 30-31 7.366448072272934 1107 32-33 6.981904772234266 1107 34-35 9.391190100056637 1107 36-37 -0.10509362160232882 1107 38-39 0.86976774351389 1107 40-41 6.990719979294145 1107 42-43 7.994173684874752 1107 44-45 -3.142862646092233 1107 46-47 -4.305055986412917 1107 48-49 4.799803568786974 1107 50-51 -9.705098104649501 1107 52-53 0.44588767571397625 1107 54-55 5.329358250850088 1107 56-57 6.368423386269804 1107 58-59 6.771827489069853 1107 60-61 6.78492963960629 1107 62-63 6.5877764757936035 1107 64-65 6.675690682879424 1107 66-67 6.450384621835251 1107 68-69 6.403093344455744 1107 70-71 6.363358243970087 1107 72-73 6.151812001457667 1107 74-75 6.192449938200706 1107 76-77 5.987757430635174 1107 78-79 6.04270000243077 1107 80-81 5.96902222510856 1107 82-83 5.74490741634288 1107 84-85 5.717752548966006 1107 86-87 5.526167690639694 1107 88-89 5.228608315959995 1107 90-91 5.114317807606737 1107 92-93 4.928407627895945 1107 94-95 4.5673397738417325 1107 96-97 4.413769510503503 1107 98-99 4.196514942414552 1107 100-101 3.9831050980416975 1108 1 6.011472162657139 1108 2 6.4970062806569615 1108 3 6.615231269565722 1108 4 8.030168004109616 1108 5 8.129640184406032 1108 6 7.4867742689662045 1108 7 7.550076385178524 1108 8 6.366113690617773 1108 9 5.145068776390733 1108 10-11 5.770606400523558 1108 12-13 5.480022099265572 1108 14-15 6.155942379634791 1108 16-17 6.263442157933966 1108 18-19 7.003371564974998 1108 20-21 7.760994892750631 1108 22-23 7.681680231642648 1108 24-25 7.30817639410002 1108 26-27 7.6674219195660065 1108 28-29 7.640934441023962 1108 30-31 7.3922135573885654 1108 32-33 7.398860415713834 1108 34-35 9.516661365973928 1108 36-37 -3.6430797398689965 1108 38-39 -2.442519930191775 1108 40-41 5.3951009994830095 1108 42-43 7.783862674548288 1108 44-45 -3.1427466292618504 1108 46-47 -4.212708397148393 1108 48-49 5.084228609518757 1108 50-51 -9.819493669229878 1108 52-53 0.5060392710390076 1108 54-55 5.319942100222665 1108 56-57 6.460289993126793 1108 58-59 6.672124419846863 1108 60-61 6.763527278260284 1108 62-63 6.983090557951339 1108 64-65 6.897201610670599 1108 66-67 6.7822220676004505 1108 68-69 6.511643972082908 1108 70-71 6.414045977795322 1108 72-73 6.393579002991391 1108 74-75 6.33725211304175 1108 76-77 6.18359435681025 1108 78-79 6.1527363814867435 1108 80-81 6.087309227892188 1108 82-83 5.957953763936793 1108 84-85 5.922944098757172 1108 86-87 5.983922973505125 1108 88-89 5.776578596024752 1108 90-91 5.64016773902962 1108 92-93 5.406768326223007 1108 94-95 5.581356634179679 1108 96-97 5.267115650235542 1108 98-99 5.108047751354395 1108 100-101 4.831443339332431 1109 1 4.9859323449974475 1109 2 6.610043335018382 1109 3 6.125595106508165 1109 4 7.906491049963819 1109 5 7.59451105427631 1109 6 5.102821583040281 1109 7 4.745062987854141 1109 8 3.625530530557924 1109 9 2.98758747337412 1109 10-11 2.8620823862601306 1109 12-13 3.385873723764572 1109 14-15 4.008705971591478 1109 16-17 3.259298278466094 1109 18-19 3.9296970937928677 1109 20-21 4.639549669064969 1109 22-23 6.011894508737122 1109 24-25 5.910695160499596 1109 26-27 4.577143496573662 1109 28-29 4.441139966338973 1109 30-31 4.460151047576851 1109 32-33 4.306328102478091 1109 34-35 6.591668627975185 1109 36-37 -4.7623215335405895 1109 38-39 -3.484491627609815 1109 40-41 3.0884079973823653 1109 42-43 3.8243264387639186 1109 44-45 -4.744853303788567 1109 46-47 -6.163804558860345 1109 48-49 1.8771131298155161 1109 50-51 -10.066220295464285 1109 52-53 -1.208879389207194 1109 54-55 2.2325917054581446 1109 56-57 3.5821720048292924 1109 58-59 4.473181761890327 1109 60-61 4.613809508463586 1109 62-63 4.8150207498283635 1109 64-65 4.4955723593295325 1109 66-67 4.744846255831522 1109 68-69 4.7727520306616285 1109 70-71 4.581300946659493 1109 72-73 4.321075929990119 1109 74-75 4.391171404441295 1109 76-77 4.60093756432941 1109 78-79 4.751173209017416 1109 80-81 4.480234757137229 1109 82-83 4.2177068719131405 1109 84-85 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76-77 -13.968864032160617 2313 78-79 -13.49834670797293 2313 80-81 -12.948478147515813 2313 82-83 -12.436140776672076 2313 84-85 -11.923435219806576 2313 86-87 -11.366962916634197 2313 88-89 -10.824268528202055 2313 90-91 -10.259049381312375 2313 92-93 -9.663376195290569 2313 94-95 -9.16057293295165 2313 96-97 -8.398135489865837 2313 98-99 -7.580693340019739 2313 100-101 -6.534154968008107 2314 1 -20.86050142501711 2314 2 -19.701819838198503 2314 3 -20.135685825078443 2314 4 -22.054571541011438 2314 5 -22.031034797252072 2314 6 -22.83604304578068 2314 7 -22.912506153339454 2314 8 -23.730364665948628 2314 9 -24.889413982229957 2314 10-11 -25.166847744744235 2314 12-13 -25.14360225876818 2314 14-15 -25.39431440686558 2314 16-17 -24.504166059234052 2314 18-19 -23.85833056268457 2314 20-21 -23.340435774894274 2314 22-23 -23.23064552037496 2314 24-25 -22.954479511989486 2314 26-27 -22.60917382511484 2314 28-29 -22.5424844878095 2314 30-31 -22.439958122728825 2314 32-33 -22.136539437551022 2314 34-35 -19.296067907687114 2314 36-37 -17.582918331211623 2314 38-39 -16.66243922586309 2314 40-41 -17.981824899269743 2314 42-43 -17.91184532251701 2314 44-45 -15.919525792871536 2314 46-47 -16.672356232805033 2314 48-49 -16.172741309776914 2314 50-51 -14.115164982509409 2314 52-53 -16.975618836077647 2314 54-55 -18.590916300364274 2314 56-57 -18.770084181488027 2314 58-59 -18.38216758308784 2314 60-61 -18.051219603617927 2314 62-63 -17.578538202136702 2314 64-65 -17.088124875022725 2314 66-67 -16.659083875172843 2314 68-69 -16.191767619993392 2314 70-71 -15.603378820443854 2314 72-73 -15.139986660985134 2314 74-75 -14.609556397596549 2314 76-77 -14.050081358523574 2314 78-79 -13.574336252409076 2314 80-81 -13.082720119136425 2314 82-83 -12.596521657926743 2314 84-85 -12.085309753040333 2314 86-87 -11.53331840580522 2314 88-89 -10.982408931488088 2314 90-91 -10.420177088556587 2314 92-93 -9.815168577665476 2314 94-95 -9.16057293295165 2314 96-97 -8.440331158275097 2314 98-99 -7.684688859063801 2314 100-101 -6.656634430293394 2315 1 -20.86050142501711 2315 2 -19.701819838198503 2315 3 -20.135685825078443 2315 4 -16.408944164585584 2315 5 -22.031034797252072 2315 6 -22.83604304578068 2315 7 -22.912506153339454 2315 8 -23.730364665948628 2315 9 -19.719452005043646 2315 10-11 -17.878824931056023 2315 12-13 -14.13105473025107 2315 14-15 -14.092983608386492 2315 16-17 -20.026599519310096 2315 18-19 -23.85833056268457 2315 20-21 -23.340435774894274 2315 22-23 -23.23064552037496 2315 24-25 -22.954479511989486 2315 26-27 -22.60917382511484 2315 28-29 -22.5424844878095 2315 30-31 -22.439958122728825 2315 32-33 -22.136539437551022 2315 34-35 -19.296067907687114 2315 36-37 -17.582918331211623 2315 38-39 -16.66243922586309 2315 40-41 -17.981824899269743 2315 42-43 -17.91184532251701 2315 44-45 -15.919525792871536 2315 46-47 -16.672356232805033 2315 48-49 -16.172741309776914 2315 50-51 -14.115164982509409 2315 52-53 -16.975618836077647 2315 54-55 -18.590916300364274 2315 56-57 -18.770084181488027 2315 58-59 -18.38216758308784 2315 60-61 -18.051219603617927 2315 62-63 -17.578538202136702 2315 64-65 -17.088124875022725 2315 66-67 -16.659083875172843 2315 68-69 -16.191767619993392 2315 70-71 -15.603378820443854 2315 72-73 -15.139986660985134 2315 74-75 -14.609556397596549 2315 76-77 -14.050081358523574 2315 78-79 -13.574336252409076 2315 80-81 -13.082720119136425 2315 82-83 -12.596521657926743 2315 84-85 -12.085309753040333 2315 86-87 -11.53331840580522 2315 88-89 -10.982408931488088 2315 90-91 -10.420177088556587 2315 92-93 -9.815168577665476 2315 94-95 -9.16057293295165 2315 96-97 -8.440331158275097 2315 98-99 -7.684688859063801 2315 100-101 -6.656634430293394 2316 1 -20.86050142501711 2316 2 -19.701819838198503 2316 3 -20.135685825078443 2316 4 -22.054571541011438 2316 5 -22.031034797252072 2316 6 -22.83604304578068 2316 7 -22.912506153339454 2316 8 -23.730364665948628 2316 9 -24.889413982229957 2316 10-11 -25.166847744744235 2316 12-13 -25.14360225876818 2316 14-15 -25.39431440686558 2316 16-17 -24.504166059234052 2316 18-19 -23.85833056268457 2316 20-21 -23.340435774894274 2316 22-23 -23.23064552037496 2316 24-25 -22.954479511989486 2316 26-27 -22.60917382511484 2316 28-29 -22.5424844878095 2316 30-31 -22.439958122728825 2316 32-33 -22.136539437551022 2316 34-35 -19.296067907687114 2316 36-37 -17.582918331211623 2316 38-39 -16.66243922586309 2316 40-41 -17.981824899269743 2316 42-43 -17.91184532251701 2316 44-45 -15.919525792871536 2316 46-47 -16.672356232805033 2316 48-49 -16.172741309776914 2316 50-51 -14.115164982509409 2316 52-53 -16.975618836077647 2316 54-55 -18.590916300364274 2316 56-57 -18.770084181488027 2316 58-59 -18.38216758308784 2316 60-61 -18.051219603617927 2316 62-63 -17.578538202136702 2316 64-65 -17.088124875022725 2316 66-67 -16.659083875172843 2316 68-69 -16.191767619993392 2316 70-71 -15.603378820443854 2316 72-73 -15.139986660985134 2316 74-75 -14.609556397596549 2316 76-77 -14.050081358523574 2316 78-79 -13.574336252409076 2316 80-81 -13.082720119136425 2316 82-83 -12.596521657926743 2316 84-85 -12.085309753040333 2316 86-87 -11.53331840580522 2316 88-89 -10.982408931488088 2316 90-91 -10.420177088556587 2316 92-93 -9.815168577665476 2316 94-95 -9.16057293295165 2316 96-97 -8.440331158275097 2316 98-99 -7.684688859063801 2316 100-101 -6.656634430293394 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 400383.0 3 25017.0 4 54201.0 5 32464.0 6 28436.0 7 35016.0 8 45290.0 9 54918.0 10 57445.0 11 54642.0 12 48605.0 13 45161.0 14 42730.0 15 45004.0 16 49392.0 17 51718.0 18 53812.0 19 55763.0 20 58821.0 21 62446.0 22 65875.0 23 70287.0 24 74353.0 25 79558.0 26 84817.0 27 89637.0 28 95074.0 29 101185.0 30 105266.0 31 105364.0 32 102184.0 33 99697.0 34 93101.0 35 78335.0 36 55390.0 37 23851.0 38 3355.0 39 29.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.75089594677732 19.554424029177987 13.544000549995857 24.150679474048832 2 15.747114911740411 20.224934229059535 40.01107721102565 24.016873648174407 3 19.057500064659838 25.130442713426245 29.560254119966007 26.251803101947917 4 12.80489012531091 16.415869333873072 37.74799759049065 33.03124295032537 5 13.556828829106726 37.45257355918088 34.86918206740724 14.121415544305163 6 31.706486418088993 36.76018318012982 17.419477292286818 14.11385310949437 7 27.58675042549461 32.15534936605288 22.235170560909047 18.022729647543468 8 25.925775022827267 35.325592576215634 20.282667872112388 18.465964528844705 9 26.41041078816083 15.822523667999738 20.561682424455004 37.20538311938443 10-11 25.3512111998694 26.017023253797905 28.155594070967155 20.476171475365543 12-13 25.658989529388045 23.677019244483134 28.345072812310963 22.318918413817865 14-15 22.724365719749986 25.115638530239288 25.96281668039059 26.197179069620134 16-17 22.57484128517324 28.081042112575687 26.69671448474559 22.64740211750548 18-19 22.577156565539692 26.945595075555968 28.598250306069712 21.878998052834632 20-21 23.099679438929574 26.315761509647828 28.5810744994554 22.0034845519672 22-23 22.91945969696491 26.122038288159168 28.452358520057576 22.50614349481835 24-25 22.696799512460927 26.460876168590797 28.41563818513179 22.42668613381648 26-27 22.52832962738185 26.64589947622841 28.257089500352834 22.568681396036904 28-29 22.575659622392237 26.83460303537298 28.310164655177083 22.2795726870577 30-31 22.794240898224412 26.58264170307027 28.163573316304554 22.45954408240076 32-33 22.583342972170062 26.797048237038844 27.954654612172625 22.664954178618466 34-35 23.020762226428374 26.67983476298242 28.04292137966435 22.256481630924856 36-37 23.561938412874056 26.52871508446063 27.56310517409741 22.346241328567906 38-39 23.246154879441196 26.63054481954341 27.686846298976057 22.436454002039333 40-41 23.198486025995123 26.557121942400347 27.843852823091503 22.400539208513027 42-43 23.351071201417806 26.622037480806664 27.431094439921765 22.59579687785376 44-45 23.48792512088592 26.90052350636379 27.259918375222743 22.351632997527553 46-47 23.06957072572958 26.874774767767747 27.390335128277588 22.66531937822509 48-49 23.51921795689081 27.02223521686406 26.814717710045873 22.64382911619926 50-51 23.712661879557036 27.026783535190212 26.78270100521675 22.477853580035998 52-53 22.82517953831578 27.05635353903863 27.2850007553581 22.83346616728749 54-55 22.571414179868377 27.027362401628974 27.44446440527078 22.956759013231867 56-57 22.655636103231966 27.2524951022664 27.21053910552654 22.8813296889751 58-59 22.640871506382947 27.036910042574714 27.380012519544124 22.942205931498222 60-61 22.511169973727878 27.216926507190266 27.394220132743364 22.877683386338497 62-63 22.5813033495542 27.095258062106254 27.39917806006803 22.92426052827151 64-65 22.332836392581594 27.04888642809869 27.31886444049155 23.299412738828174 66-67 22.386870016855305 27.1630547132668 27.20056098575662 23.249514284121272 68-69 22.56676032732361 26.989992565558847 27.32820758833495 23.11503951878259 70-71 22.375551015330313 26.88873583798151 27.208860193981337 23.526852952706843 72-73 22.48927151598107 27.017078116270106 27.13077372451189 23.362876643236937 74-75 22.49228457365204 27.28226603622999 26.963768306872954 23.26168108324501 76-77 22.45908649488059 27.06074079051422 26.989697811106478 23.49047490349871 78-79 22.447448008463404 27.128687926946565 26.92613417398016 23.497729890609868 80-81 22.5343596883885 27.20795598750037 26.853741416747308 23.40394290736383 82-83 22.60478497733712 27.10424632541048 26.96609003839589 23.324878658856512 84-85 22.585182134807923 27.163476573732304 26.692466141861853 23.558875149597917 86-87 22.402973022349173 27.23461787425202 26.737376228309014 23.625032875089797 88-89 22.464648791867063 27.103883539675376 26.555586488196 23.875881180261562 90-91 22.39277164516558 27.094942811544087 26.694519716717647 23.817765826572682 92-93 22.52832049304009 26.926940758480438 26.720948451831443 23.823790296648024 94-95 22.22518604607171 26.776330038387776 26.87265549430241 24.125828421238104 96-97 22.3660982647589 26.567730412597264 26.699209223483944 24.3669620991599 98-99 22.070863038001352 26.74740720394368 26.642709399304 24.539020358750964 100-101 22.427752949256217 26.767206672914206 26.391339319243663 24.413701058585914 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 28560.0 1 30808.0 2 22957.5 3 13518.5 4 17464.5 5 22402.5 6 23930.5 7 22883.0 8 21143.5 9 19439.0 10 17436.5 11 15437.5 12 13383.5 13 11474.5 14 9883.5 15 8467.0 16 7547.0 17 6992.5 18 6721.0 19 6754.5 20 7180.5 21 7952.5 22 8980.0 23 10448.0 24 12447.0 25 14861.5 26 17963.5 27 21599.0 28 25370.5 29 30040.0 30 35427.5 31 40576.5 32 45910.0 33 50193.5 34 53728.5 35 57468.0 36 61371.5 37 65399.0 38 68165.5 39 69780.0 40 71256.5 41 72629.0 42 74200.0 43 75829.0 44 77271.5 45 79647.0 46 81348.5 47 81992.0 48 82283.0 49 81437.0 50 80164.5 51 78465.0 52 76962.5 53 75593.0 54 72783.5 55 67824.0 56 61669.5 57 55324.5 58 48744.5 59 42362.5 60 36161.5 61 30042.5 62 24449.0 63 19535.0 64 15167.5 65 11613.0 66 8901.5 67 6893.5 68 5255.0 69 3889.5 70 2913.5 71 2240.5 72 1681.0 73 1252.0 74 964.0 75 697.5 76 486.5 77 322.0 78 202.0 79 133.0 80 95.0 81 55.0 82 33.5 83 22.5 84 11.5 85 10.5 86 9.5 87 3.0 88 2.5 89 2.5 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.676899911816914 2 20.32365246886011 3 16.159835837940946 4 21.2465314526014 5 21.06921421185701 6 15.216946369618759 7 14.078631313288865 8 11.297972854217914 9 2.8196522740812484 10-11 6.089026874156877 12-13 3.3091292700129573 14-15 6.906926899341176 16-17 10.792460840699043 18-19 13.693087100389482 20-21 14.078935655259675 22-23 12.076194294957586 24-25 14.510169206527221 26-27 12.180260227602142 28-29 12.17404023857367 30-31 12.962323985723318 32-33 11.525468477399945 34-35 18.32772076015494 36-37 22.920773698158197 38-39 22.117463066199704 40-41 17.199068561398327 42-43 17.0994536300769 44-45 22.32034503249231 46-47 11.958509059119189 48-49 20.630277004453283 50-51 20.191796310005774 52-53 10.478246777208742 54-55 12.0123205238334 56-57 11.548522381688962 58-59 12.516729297708077 60-61 11.959954683480547 62-63 11.90285252120693 64-65 12.9454710490896 66-67 11.29915217935481 68-69 12.292980885041668 70-71 11.01455439389916 72-73 10.39365873069616 74-75 10.482450500680585 76-77 10.546533506909704 78-79 10.909689563581223 80-81 10.873872317891276 82-83 9.475325855143874 84-85 9.373846829251221 86-87 9.522156475902584 88-89 10.835315994464018 90-91 8.466108858557831 92-93 10.383824680764294 94-95 10.506664708733322 96-97 10.07155079733792 98-99 9.905475188140402 100-101 10.041687241451985 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2628622.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.52623733131989 #Duplication Level Percentage of deduplicated Percentage of total 1 98.7794958353352 95.34813058469692 2 0.8672912288114774 1.6743271797525745 3 0.1680211348435505 0.4865534381575877 4 0.059352509496380365 0.22916297671428115 5 0.030054952283502412 0.14505457285494242 6 0.019143983675856024 0.11087380270575553 7 0.013934284110062688 0.09415168105349653 8 0.009792039471149726 0.07561509807598804 9 0.009131954359310518 0.07933258744070296 >10 0.0373618610337372 0.6675774035637236 >50 0.0038460827974356807 0.2534714977815382 >100 0.002297856843607067 0.4461300865439145 >500 1.5787253645562467E-4 0.08773763236168247 >1k 1.1840440227184138E-4 0.3018814582969218 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4803 0.18271931072630448 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.804274635151041E-5 5 0.0 0.0 0.0 0.0 3.804274635151041E-5 6 0.0 0.0 0.0 0.0 3.804274635151041E-5 7 0.0 0.0 0.0 0.0 3.804274635151041E-5 8 0.0 0.0 0.0 0.0 3.804274635151041E-5 9 0.0 0.0 0.0 0.0 3.804274635151041E-5 10-11 0.0 0.0 0.0 0.0 3.804274635151041E-5 12-13 0.0 0.0 0.0 0.0 9.510686587877604E-5 14-15 0.0 0.0 0.0 0.0 2.0923510493330727E-4 16-17 0.0 0.0 0.0 0.0 3.043419708120833E-4 18-19 0.0 0.0 0.0 0.0 3.804274635151041E-4 20-21 0.0 0.0 0.0 3.804274635151041E-5 4.565129562181249E-4 22-23 0.0 0.0 0.0 1.7119235858179685E-4 5.135770757453905E-4 24-25 0.0 0.0 0.0 4.3749158304236973E-4 5.516198220969009E-4 26-27 0.0 0.0 0.0 7.228121806786979E-4 5.706411952726562E-4 28-29 0.0 0.0 0.0 0.001293453375951354 5.706411952726562E-4 30-31 0.0 3.804274635151041E-5 0.0 0.0040515524864358585 5.706411952726562E-4 32-33 0.0 3.804274635151041E-5 0.0 0.01067099035159867 5.706411952726562E-4 34-35 0.0 3.804274635151041E-5 0.0 0.020086570073597498 5.706411952726562E-4 36-37 0.0 3.804274635151041E-5 0.0 0.0352466044946744 5.706411952726562E-4 38-39 0.0 3.804274635151041E-5 0.0 0.05930864156200473 5.706411952726562E-4 40-41 0.0 3.804274635151041E-5 0.0 0.08323752901710478 5.706411952726562E-4 42-43 0.0 3.804274635151041E-5 0.0 0.10935387438741667 5.706411952726562E-4 44-45 0.0 3.804274635151041E-5 0.0 0.141576080547146 5.706411952726562E-4 46-47 0.0 3.804274635151041E-5 0.0 0.17908622844973526 5.706411952726562E-4 48-49 1.9021373175755206E-5 3.804274635151041E-5 0.0 0.22146584788531787 6.086839416241666E-4 50-51 3.804274635151041E-5 3.804274635151041E-5 0.0 0.2676687633292273 6.086839416241666E-4 52-53 3.804274635151041E-5 3.804274635151041E-5 0.0 0.32785238805731676 6.086839416241666E-4 54-55 3.804274635151041E-5 3.804274635151041E-5 0.0 0.393875574350363 6.847694343271874E-4 56-57 3.804274635151041E-5 3.804274635151041E-5 3.804274635151041E-5 0.4730425295078562 6.847694343271874E-4 58-59 3.804274635151041E-5 3.804274635151041E-5 3.804274635151041E-5 0.5580300248571304 6.847694343271874E-4 60-61 3.804274635151041E-5 3.804274635151041E-5 3.804274635151041E-5 0.64881903902501 7.228121806786978E-4 62-63 3.804274635151041E-5 3.804274635151041E-5 3.804274635151041E-5 0.7425563660351318 7.228121806786978E-4 64-65 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 0.8402311172926347 7.608549270302082E-4 66-67 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 0.9497942267849847 7.608549270302082E-4 68-69 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 1.0690772579701457 7.608549270302082E-4 70-71 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 1.1994877924631233 7.608549270302082E-4 72-73 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 1.3401888898441845 7.608549270302082E-4 74-75 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 1.4832296161258638 7.608549270302082E-4 76-77 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 1.6385010853595534 7.608549270302082E-4 78-79 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 1.8119189446029136 7.608549270302082E-4 80-81 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 1.9970159269761876 7.608549270302082E-4 82-83 3.804274635151041E-5 7.608549270302083E-5 3.804274635151041E-5 2.192669771462006 7.988976733817187E-4 84-85 3.804274635151041E-5 9.510686587877604E-5 3.804274635151041E-5 2.390168689145872 7.988976733817187E-4 86-87 3.804274635151041E-5 1.1412823905453123E-4 3.804274635151041E-5 2.6015722306212155 8.369404197332291E-4 88-89 3.804274635151041E-5 1.1412823905453123E-4 3.804274635151041E-5 2.8296004522521683 8.749831660847395E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2205 0.0 53.036633 1 GTATCAA 4235 0.0 46.81363 1 ATCAACG 5165 0.0 37.39299 3 TATCAAC 5225 0.0 37.356064 2 TCAACGC 5305 0.0 36.39158 4 CAACGCA 5370 0.0 34.673485 5 AACGCAG 6025 0.0 31.446943 6 GTACATG 5825 0.0 28.434568 1 ACGCAGA 6825 0.0 27.50954 7 TACATGG 6010 0.0 26.549143 2 CGCAGAG 7125 0.0 26.15558 8 ACATGGG 6190 0.0 25.690254 3 GTACTAG 415 0.0 25.398018 1 GCAGAGT 7655 0.0 23.14873 9 CATGGGG 4645 0.0 22.886305 4 GTGGTAT 1010 0.0 22.859415 1 GAGTACA 4390 0.0 20.808247 1 CAGAGTA 7310 0.0 19.600088 10-11 GAGTACT 4890 0.0 19.420662 12-13 GTACTTT 5135 0.0 18.981434 14-15 >>END_MODULE