FastQCFastQC Report
Sat 18 Jun 2016
SRR1930917_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930917_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628622
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49230.18728444028848573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43700.1662468015561005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26590.10115566254866619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30350.054.6036871
GTATCAA58500.043.1016461
CAACGCA73400.033.621315
ATCAACG73900.033.4084473
TCAACGC75000.033.1704144
TATCAAC77300.032.308752
AACGCAG80100.030.9867866
ACGCAGA92000.026.720797
CGCAGAG94400.025.8403558
GTACATG84050.024.745241
TACATGG83200.024.424992
ACATGGG80350.024.2267633
GCAGAGT106050.022.643699
TCTAACG2554.9294613E-1022.3436952
GTGGTAT14500.021.9412861
CATGGGG58050.019.6286264
AGAGTAC100900.019.04895610-11
GAGTACA58350.018.7172581
CTAACGC2552.6908128E-718.6190383
CAGAGTA103300.018.58341610-11
Produced by FastQC (version 0.11.5)