##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930917_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2628622 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.223601948092956 31.0 31.0 33.0 30.0 34.0 2 31.34383909135661 31.0 31.0 34.0 30.0 34.0 3 31.423620056440217 31.0 31.0 34.0 28.0 34.0 4 35.293711305771616 37.0 35.0 37.0 33.0 37.0 5 35.078912068756935 35.0 35.0 37.0 32.0 37.0 6 35.121241471767334 37.0 35.0 37.0 32.0 37.0 7 34.99545237010114 36.0 35.0 37.0 32.0 37.0 8 34.97010524906206 36.0 35.0 37.0 32.0 37.0 9 36.475307594625626 38.0 35.0 39.0 32.0 39.0 10-11 36.39652848526718 38.0 35.0 39.0 32.0 39.0 12-13 36.2888363180404 38.0 35.0 39.0 32.0 39.0 14-15 37.29588620958053 39.0 36.0 41.0 32.0 41.0 16-17 37.25479262518536 39.0 36.0 40.0 32.0 41.0 18-19 37.24424622482806 39.0 36.0 40.0 31.0 41.0 20-21 37.1980459723764 39.0 36.0 40.0 31.0 41.0 22-23 37.07008672224458 39.0 36.0 40.0 31.0 41.0 24-25 36.94784491646193 39.0 36.0 40.0 31.0 41.0 26-27 36.65617840830671 38.5 35.0 40.0 30.0 41.0 28-29 36.4625592801095 38.0 35.0 40.0 30.0 41.0 30-31 36.20809610510754 38.0 35.0 40.0 30.0 41.0 32-33 35.95665961100531 38.0 34.0 40.0 29.0 41.0 34-35 36.14951978641281 38.0 35.0 40.0 30.0 41.0 36-37 36.19636543405632 38.0 35.0 40.0 30.0 41.0 38-39 36.14546271772815 38.0 35.0 40.0 30.0 41.0 40-41 36.0324310608372 38.0 35.0 40.0 29.5 41.0 42-43 35.87171491374568 38.0 34.5 40.0 29.0 41.0 44-45 35.669112561638755 38.0 34.0 40.0 28.0 41.0 46-47 35.423098300174004 38.0 34.0 40.0 27.0 41.0 48-49 35.18855335609304 38.0 34.0 40.0 27.0 41.0 50-51 34.88827301909518 38.0 33.0 40.0 26.0 41.0 52-53 34.65626000999764 38.0 33.0 40.0 26.0 41.0 54-55 34.45877497791619 37.0 33.0 40.0 26.0 41.0 56-57 34.17479044153173 37.0 33.0 40.0 25.0 41.0 58-59 33.9047067246641 37.0 32.5 40.0 24.0 41.0 60-61 33.571446750426645 36.0 32.0 39.0 23.5 41.0 62-63 33.24881610973354 36.0 31.5 39.0 23.0 41.0 64-65 32.91815616699548 35.5 31.0 39.0 22.5 40.0 66-67 32.56001794856773 35.0 31.0 38.5 22.0 40.0 68-69 32.182788168097204 35.0 31.0 38.0 21.5 40.0 70-71 31.770177302023647 35.0 30.5 37.5 20.0 40.0 72-73 31.340501601219195 34.0 30.0 37.0 19.5 39.0 74-75 30.81349125130962 34.0 29.5 36.0 18.0 39.0 76-77 29.47725062789553 32.5 28.0 35.0 16.0 37.5 78-79 30.243356404990905 34.0 29.0 35.0 18.0 37.5 80-81 30.250618575055675 34.0 29.0 35.0 18.0 37.0 82-83 29.983639907145268 34.0 29.0 35.0 16.0 37.0 84-85 29.671519145772955 34.0 29.0 35.0 12.0 36.0 86-87 29.339246190589595 34.0 29.0 35.0 7.5 36.0 88-89 28.991619182978763 34.0 29.0 35.0 6.0 36.0 90-91 28.645964691766256 33.0 29.0 35.0 2.0 35.0 92-93 28.279195905687466 33.0 28.5 35.0 2.0 35.0 94-95 27.846597951322025 33.0 27.0 35.0 2.0 35.0 96-97 27.371036611578234 33.0 26.5 35.0 2.0 35.0 98-99 26.85767447734973 33.0 25.5 35.0 2.0 35.0 100-101 25.623256976469037 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.48913106545105123 1101 2 0.5809761772342199 1101 3 0.49800875237943387 1101 4 0.3828879226220536 1101 5 0.4952274529420606 1101 6 0.48036618775726936 1101 7 0.5862707021960318 1101 8 0.517132220086836 1101 9 0.7514263271439532 1101 10-11 0.7209554499644 1101 12-13 0.6361672005883037 1101 14-15 0.8788084253318829 1101 16-17 0.8792704324241143 1101 18-19 0.7793366335017566 1101 20-21 0.8411393760014363 1101 22-23 0.8783264167774192 1101 24-25 0.7247614927237365 1101 26-27 0.7370827740781465 1101 28-29 0.7141682105435194 1101 30-31 0.9100732733005259 1101 32-33 0.8423777337065275 1101 34-35 0.8422177342176695 1101 36-37 0.7605649473325755 1101 38-39 0.8710313797453964 1101 40-41 0.9676070068902405 1101 42-43 0.950843412112583 1101 44-45 0.9311651947517063 1101 46-47 1.014998207556495 1101 48-49 1.0110639391635914 1101 50-51 0.9430047179426353 1101 52-53 0.8757631057480566 1101 54-55 0.9193321597451671 1101 56-57 1.1425573508263511 1101 58-59 1.0387964626013826 1101 60-61 0.9849292514383308 1101 62-63 0.9517622706611846 1101 64-65 0.9883470273758945 1101 66-67 1.082108809524506 1101 68-69 1.031663518995643 1101 70-71 0.8743408527753509 1101 72-73 0.9418986970559509 1101 74-75 0.9014798194838818 1101 76-77 1.039124970117168 1101 78-79 1.0640188316550088 1101 80-81 1.0440259417275506 1101 82-83 1.0918364968229746 1101 84-85 1.004739587152251 1101 86-87 0.9497901970611551 1101 88-89 0.798745404092994 1101 90-91 0.9362851397954088 1101 92-93 0.9670044104040443 1101 94-95 1.050138768310763 1101 96-97 1.0786178820436874 1101 98-99 1.0717177947268368 1101 100-101 1.1609889532238604 1102 1 0.4662231061795161 1102 2 0.5000329358894327 1102 3 0.4600325793143547 1102 4 0.36010999492888374 1102 5 0.39693156183694356 1102 6 0.40844070074394523 1102 7 0.48824671870823977 1102 8 0.47103435188375187 1102 9 0.568412931573981 1102 10-11 0.6461060548558564 1102 12-13 0.602133430486198 1102 14-15 0.7514717192454796 1102 16-17 0.6655846474247582 1102 18-19 0.6656175810174076 1102 20-21 0.6780984446354523 1102 22-23 0.7768936747744917 1102 24-25 0.6438330569547048 1102 26-27 0.7790877374221168 1102 28-29 0.7746845208807471 1102 30-31 0.8079884422437331 1102 32-33 0.8349593677889118 1102 34-35 0.8357036671182883 1102 36-37 0.8578565054250262 1102 38-39 0.838469155129097 1102 40-41 0.7752878563543604 1102 42-43 0.7251885421853572 1102 44-45 0.7234850662547601 1102 46-47 0.7378450391117823 1102 48-49 0.5789588334801081 1102 50-51 0.6922157738711476 1102 52-53 0.766090867721779 1102 54-55 0.7364055377235275 1102 56-57 0.6566902384646482 1102 58-59 0.766905582012086 1102 60-61 0.7279593013498555 1102 62-63 0.6418465517230274 1102 64-65 0.6072984990758741 1102 66-67 0.6726079556933868 1102 68-69 0.7966748278806364 1102 70-71 0.6263311083891061 1102 72-73 0.6232377751243412 1102 74-75 0.8446382530488172 1102 76-77 0.8956272700024321 1102 78-79 0.929371003517101 1102 80-81 0.6665420810925688 1102 82-83 0.994912760729914 1102 84-85 1.1053460492839662 1102 86-87 0.9060160317866419 1102 88-89 1.0110465458989957 1102 90-91 0.9407198603382341 1102 92-93 1.0408207625573667 1102 94-95 1.0755856907079178 1102 96-97 1.0949851241794768 1102 98-99 1.198626297813611 1102 100-101 1.3287106351784637 1103 1 0.4861925895037871 1103 2 0.5302372662637573 1103 3 0.5069304579115474 1103 4 0.32421446235841245 1103 5 0.3557765611547197 1103 6 0.445678529202965 1103 7 0.45898375192121676 1103 8 0.44461736803508956 1103 9 0.6006392335475397 1103 10-11 0.5124855012587162 1103 12-13 0.5344005639144314 1103 14-15 0.6144597875001949 1103 16-17 0.6997913004062681 1103 18-19 0.65495443644555 1103 20-21 0.598362937765387 1103 22-23 0.6482479909952659 1103 24-25 0.5951783311974879 1103 26-27 0.7747832518679019 1103 28-29 0.6655104006916801 1103 30-31 0.7868441636367649 1103 32-33 0.7682204314839396 1103 34-35 0.7045136542273625 1103 36-37 0.8126408878518205 1103 38-39 0.8608238674455393 1103 40-41 0.8434576754944345 1103 42-43 0.8445211413671032 1103 44-45 0.8850751663070966 1103 46-47 0.8412962643608992 1103 48-49 0.8709787024121596 1103 50-51 0.8114308935246157 1103 52-53 0.6701285487395268 1103 54-55 0.7708106203741352 1103 56-57 0.8082534671431958 1103 58-59 0.752839858643668 1103 60-61 0.6851878434530931 1103 62-63 0.5755323490971875 1103 64-65 0.5466483879858046 1103 66-67 0.4878499507936098 1103 68-69 0.6824513790176994 1103 70-71 0.49014815190668415 1103 72-73 0.39262927953434357 1103 74-75 0.5841218624272209 1103 76-77 0.649833622602916 1103 78-79 0.7842044906047896 1103 80-81 0.6712854284165282 1103 82-83 0.7857045040192006 1103 84-85 0.8726707795010711 1103 86-87 0.8974817740943379 1103 88-89 0.7213407563856542 1103 90-91 0.7500810058998262 1103 92-93 0.8690317084419448 1103 94-95 0.9377908590943917 1103 96-97 0.8866248074658714 1103 98-99 0.8425604848399999 1103 100-101 1.167134154096356 1104 1 0.34471225743190814 1104 2 0.5067540838214448 1104 3 0.3776092256286816 1104 4 0.21334286670913372 1104 5 0.344033853600493 1104 6 0.2921384509852274 1104 7 0.28184885795425174 1104 8 0.42217460801320783 1104 9 0.49963602222941006 1104 10-11 0.4301596585202745 1104 12-13 0.4199769824946813 1104 14-15 0.5461208538959639 1104 16-17 0.5786704647937597 1104 18-19 0.6838637418259026 1104 20-21 0.5201113386182641 1104 22-23 0.5406783225089811 1104 24-25 0.7139226797613318 1104 26-27 0.6226346828208307 1104 28-29 0.47428366228201213 1104 30-31 0.8015311996608361 1104 32-33 0.7519727618292222 1104 34-35 0.7871027655145966 1104 36-37 0.7392021726767553 1104 38-39 0.7456268532643904 1104 40-41 0.8752415385278098 1104 42-43 0.8165901158663402 1104 44-45 0.6861403341105685 1104 46-47 0.7182140315459193 1104 48-49 0.6958524637475847 1104 50-51 0.737740259186964 1104 52-53 0.6713300952329391 1104 54-55 0.5027637939229948 1104 56-57 0.5684770314458376 1104 58-59 0.5577526544254141 1104 60-61 0.6019209319373786 1104 62-63 0.5968537525511266 1104 64-65 0.7325822192036853 1104 66-67 0.6136938055661219 1104 68-69 0.5498715705060349 1104 70-71 0.5439818257216054 1104 72-73 0.4358181593685302 1104 74-75 0.6757298285800033 1104 76-77 0.8701168363025538 1104 78-79 0.8752317775061087 1104 80-81 0.7482632915317673 1104 82-83 0.8834344407231143 1104 84-85 0.9693692244491068 1104 86-87 0.9323189941273071 1104 88-89 0.9617931317563695 1104 90-91 0.7948779635200971 1104 92-93 0.8570709438282123 1104 94-95 0.8919177563954719 1104 96-97 0.7799400189923809 1104 98-99 0.815304840846057 1104 100-101 1.0305928516333545 1105 1 0.11854708294886507 1105 2 0.24810127014815464 1105 3 0.22448131747511368 1105 4 0.05497367900502326 1105 5 0.11508820660498031 1105 6 0.143466111799178 1105 7 0.24852613899574294 1105 8 0.2149242494695045 1105 9 0.32530269136453427 1105 10-11 0.2214838264643717 1105 12-13 0.09815358100718186 1105 14-15 0.2562798596993119 1105 16-17 0.2848187797000392 1105 18-19 0.22752739203535555 1105 20-21 0.32223297935376394 1105 22-23 0.27195407162465557 1105 24-25 0.4718689586824141 1105 26-27 0.32438056234521184 1105 28-29 0.4266586903607319 1105 30-31 0.55834204903298 1105 32-33 0.42035667389282594 1105 34-35 0.44562598507664575 1105 36-37 0.5275150671674069 1105 38-39 0.292847800115986 1105 40-41 0.2949431758568366 1105 42-43 0.17434743423093124 1105 44-45 0.1861590326894742 1105 46-47 0.331535133663607 1105 48-49 0.21052918198036963 1105 50-51 0.2457866958425896 1105 52-53 0.13126434782050467 1105 54-55 0.2230226859303741 1105 56-57 0.2369744309240076 1105 58-59 0.24390644931126104 1105 60-61 0.23807053044247795 1105 62-63 0.08924120932222479 1105 64-65 0.09447062756584046 1105 66-67 0.006931523129949824 1105 68-69 -0.004088516976160861 1105 70-71 0.10519702353158422 1105 72-73 0.16080748328222327 1105 74-75 0.28386043969646124 1105 76-77 0.23018944603174063 1105 78-79 0.21429853945530297 1105 80-81 0.2910200298084362 1105 82-83 0.3802696908150658 1105 84-85 0.4501458511020502 1105 86-87 0.2963247234212574 1105 88-89 0.3124745499355832 1105 90-91 0.2864110248292242 1105 92-93 0.4263177675382579 1105 94-95 0.33136928943209654 1105 96-97 0.3636984210105929 1105 98-99 0.5203780800323514 1105 100-101 0.8826944361373563 1106 1 0.026211292978892686 1106 2 0.0917986323655775 1106 3 0.05085070629369426 1106 4 -0.012148085803033837 1106 5 0.03206728797176339 1106 6 0.015261441708105394 1106 7 0.09200960012380932 1106 8 0.10625654059334977 1106 9 0.02777049453582947 1106 10-11 0.07089907250024652 1106 12-13 0.0797280617635252 1106 14-15 0.08218955969186936 1106 16-17 0.008199260605280756 1106 18-19 0.02266139039758741 1106 20-21 0.13397098774106553 1106 22-23 0.18795397236660705 1106 24-25 0.09234924355300933 1106 26-27 0.135745732696833 1106 28-29 0.012427319855255803 1106 30-31 0.18360247733145485 1106 32-33 0.24204716249056446 1106 34-35 0.2543673930520285 1106 36-37 0.18384165095360316 1106 38-39 0.06650037214011206 1106 40-41 0.03157386148178887 1106 42-43 0.019231798607222572 1106 44-45 -0.08084597984185393 1106 46-47 -0.178096246767673 1106 48-49 -0.04338332205198725 1106 50-51 -0.11990004576389879 1106 52-53 -0.29981347048764917 1106 54-55 0.02621929130519618 1106 56-57 0.1044244917195627 1106 58-59 0.08616084768343057 1106 60-61 -0.15301063782472824 1106 62-63 -0.16021522880278383 1106 64-65 -0.06548979328827187 1106 66-67 -0.09824887836884244 1106 68-69 -0.01893342627912631 1106 70-71 -0.1301552184595316 1106 72-73 0.01299736392442341 1106 74-75 0.17517411993666698 1106 76-77 0.06158464194230717 1106 78-79 0.11157328572692293 1106 80-81 0.08140924542172812 1106 82-83 0.10792236209723072 1106 84-85 0.08792346344974789 1106 86-87 -0.08125499319702101 1106 88-89 0.18182068755686132 1106 90-91 0.2844591153525613 1106 92-93 0.5053018614363687 1106 94-95 0.43053353668388894 1106 96-97 0.2960711846027415 1106 98-99 0.3422911547984775 1106 100-101 0.769759747931694 1107 1 0.2127994105801818 1107 2 0.2398773296367409 1107 3 0.23603272152184118 1107 4 0.12490965887545258 1107 5 0.2238059270721493 1107 6 0.2497029837892839 1107 7 0.18411319596663844 1107 8 0.11172533401301621 1107 9 0.26967938503027966 1107 10-11 0.28368719380668495 1107 12-13 0.2629776588290511 1107 14-15 0.27057467242089217 1107 16-17 0.38013167876789566 1107 18-19 0.2633136811032415 1107 20-21 0.4237720214764096 1107 22-23 0.3136374789154459 1107 24-25 0.26275236328475415 1107 26-27 0.358532742459893 1107 28-29 0.4740848162890572 1107 30-31 0.4269606351335611 1107 32-33 0.5731059075580873 1107 34-35 0.46201536726422177 1107 36-37 0.4718512472781029 1107 38-39 0.5408691081300319 1107 40-41 0.5569294627341321 1107 42-43 0.6098129505366714 1107 44-45 0.5830577605016103 1107 46-47 0.5914013356254841 1107 48-49 0.6082371229358756 1107 50-51 0.5160789460662585 1107 52-53 0.4612228584550877 1107 54-55 0.6180249935227238 1107 56-57 0.600867182395568 1107 58-59 0.7050041015414124 1107 60-61 0.5276203482995072 1107 62-63 0.36938646815343645 1107 64-65 0.39984431812542454 1107 66-67 0.35742536531851243 1107 68-69 0.5822366583834295 1107 70-71 0.4540610508303793 1107 72-73 0.4202733416194526 1107 74-75 0.5970131684555327 1107 76-77 0.4844403821375707 1107 78-79 0.63887742252086 1107 80-81 0.7133972407532454 1107 82-83 0.8786702455333817 1107 84-85 0.9808114384029736 1107 86-87 0.8860685956060337 1107 88-89 0.8238225771806071 1107 90-91 0.9281373976475287 1107 92-93 1.0593352818405997 1107 94-95 1.0049722999066226 1107 96-97 0.9549340757270706 1107 98-99 0.9470744964683853 1107 100-101 1.163257774942199 1108 1 0.2910278096925403 1108 2 0.3976906919348977 1108 3 0.3041588114796241 1108 4 0.16753115632282345 1108 5 0.19459499388364065 1108 6 0.16729552545010762 1108 7 0.2545849305996697 1108 8 0.27478056525514916 1108 9 0.33031519083269956 1108 10-11 0.2763425601629095 1108 12-13 0.41185494904777187 1108 14-15 0.5512350089717089 1108 16-17 0.495822151287598 1108 18-19 0.39728238896885415 1108 20-21 0.5077489028148818 1108 22-23 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-0.7069230522493477 2316 72-73 -0.7637675088764269 2316 74-75 -0.33017169890015907 2316 76-77 -0.8155641724283562 2316 78-79 -0.9741461112183245 2316 80-81 -0.9733310511706641 2316 82-83 -1.2121210657409982 2316 84-85 -1.1758611739524767 2316 86-87 -1.032927211718011 2316 88-89 -1.2869023859496842 2316 90-91 -0.7701728583933374 2316 92-93 -0.9987396417590304 2316 94-95 -0.6298906249861105 2316 96-97 -0.5218309547117883 2316 98-99 -0.3306602109576886 2316 100-101 -0.4617972387685292 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 27.0 4 53.0 5 105.0 6 191.0 7 533.0 8 1351.0 9 2252.0 10 3763.0 11 5534.0 12 7769.0 13 9974.0 14 11942.0 15 14679.0 16 17424.0 17 19606.0 18 17832.0 19 16058.0 20 16223.0 21 17147.0 22 19121.0 23 22005.0 24 26905.0 25 33689.0 26 42701.0 27 54064.0 28 67758.0 29 83161.0 30 100806.0 31 121577.0 32 144331.0 33 173257.0 34 211843.0 35 263248.0 36 337348.0 37 407876.0 38 307935.0 39 48510.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.08098112308709 20.731196428184134 14.236238604544447 23.951583844184327 2 16.055022137747844 21.30028738365223 38.826581428031204 23.81810905056872 3 18.99693516209097 25.48898164901671 30.230578073473936 25.283505115418386 4 13.312418157386698 17.33409900534943 37.32928881932386 32.02419401794002 5 13.28537297988252 37.934230148826735 35.118734211887876 13.661662659402868 6 31.79583515463051 37.63098598086221 16.931843730990508 13.641335133516774 7 27.328006841607504 32.467467745457505 22.2287951633974 17.97573024953759 8 25.921604551738515 35.893939866591694 19.950719426376253 18.233736155293535 9 25.984184869486747 15.561499523324388 20.309386438978294 38.14492916821057 10-11 24.9527699304046 26.365848722258278 28.270420775600297 20.410960571736826 12-13 25.367968464084985 23.717122507534366 28.57925179048186 22.335657237898793 14-15 22.478108301612025 25.394636429277394 25.87696138889502 26.250293880215565 16-17 22.27248725758211 28.32215891063835 26.82363611047918 22.58171772130036 18-19 22.2804191701964 27.193202369910928 28.677573268427338 21.84880519146534 20-21 22.827093435267603 26.417491750430454 28.714341582776072 22.04107323152587 22-23 22.576799774482513 26.26808885723731 28.68738018908013 22.467731179200044 24-25 22.51529470082342 26.62829145404609 28.56169228829039 22.2947215568401 26-27 22.4216779465641 26.76417215444139 28.425474687757983 22.388675211236528 28-29 22.373450707976048 26.906894111739238 28.487057847844117 22.232597332440594 30-31 22.64281983162061 26.596147795541814 28.397044058727722 22.363988314109847 32-33 22.464085343220436 26.83021631269024 28.181340645161534 22.524357698927787 34-35 22.69031473303375 26.79073588122472 28.298160287426317 22.220789098315215 36-37 22.806165247346026 26.520226507976663 28.253655456030863 22.419952788646448 38-39 22.582537490843542 26.62448592097302 28.3249673734221 22.46800921476135 40-41 22.645309831608255 26.65598406435735 28.377507491707476 22.321198612326917 42-43 22.629423866551388 26.720653318381427 28.046236770844924 22.603686044222258 44-45 22.546684104149328 26.88669599334205 28.113927000356675 22.452692902151945 46-47 22.89750684721997 26.903534759604465 27.738022453631224 22.460935939544346 48-49 22.735330714621472 27.012794707333367 27.543784858534202 22.708089719510962 50-51 22.741271828766042 27.034165604172976 27.573035720751882 22.651526846309096 52-53 22.911573651704572 27.006062709786544 27.49333649999448 22.589027138514403 54-55 22.641025923954423 27.056560282810928 27.57670972423195 22.7257040690027 56-57 22.71946546552145 27.17171277141951 27.38503804909525 22.723783713963787 58-59 22.628648615226563 27.06409006378091 27.626712833708282 22.68054848728425 60-61 22.58440228237002 27.22010955102047 27.589504538743466 22.605983627866046 62-63 22.62762841092257 27.116960549608766 27.579327832016265 22.676083207452404 64-65 22.340032984813355 27.067557939526825 27.520632644302843 23.071776431356973 66-67 22.323956092249006 27.21107256157807 27.47362871106428 22.991342635108644 68-69 22.558536107960972 27.019660424693807 27.554526185592763 22.867277281752454 70-71 22.483755571082344 26.900660057838355 27.533169477036957 23.082414894042344 72-73 22.490931651576705 27.075308453278662 27.463452990294673 22.97030690484996 74-75 22.448340208847313 27.242747912270556 27.33113103317294 22.977780845709194 76-77 22.42598527785319 27.183181274183244 27.31343047235987 23.077402975603697 78-79 22.363940240270058 27.19604909188235 27.32660087146119 23.1134097963864 80-81 22.50671839501119 27.309781161407347 27.19768744169909 22.98581300188237 82-83 22.56859297380909 27.15797478678943 27.337726763300314 22.935705476101166 84-85 22.555852944214298 27.213959864514536 27.025667255631898 23.20451993563927 86-87 22.39814244878115 27.340275627305864 27.159154111926327 23.10242781198666 88-89 22.411831929806407 27.20391643359091 27.0274737732708 23.35677786333188 90-91 22.339840085950996 27.197467580530077 27.194290832952728 23.268401500566203 92-93 22.427214646828805 27.0498174967934 27.263178641588663 23.259789214789134 94-95 22.196745889944147 26.885906420701495 27.400784490962526 23.516563198391832 96-97 22.25047952881776 26.690467476875718 27.374685291380807 23.684367702925716 98-99 21.988060664485044 27.029618560599435 27.186392718314007 23.79592805660152 100-101 22.0670183845376 26.867537439768824 27.16505073761081 23.900393438082766 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 270.0 1 202.0 2 169.5 3 217.0 4 216.5 5 249.5 6 398.0 7 741.5 8 1171.5 9 1406.5 10 1286.5 11 1116.0 12 1196.5 13 1339.0 14 1481.5 15 1658.0 16 1862.5 17 2124.5 18 2370.5 19 2695.0 20 3165.5 21 3770.5 22 4539.5 23 5686.5 24 7332.0 25 9682.0 26 12639.0 27 16451.5 28 20811.5 29 26314.5 30 32855.5 31 39223.0 32 47255.5 33 52151.5 34 54330.0 35 58423.5 36 64570.5 37 71672.5 38 75016.5 39 75725.0 40 77164.5 41 79336.5 42 81332.5 43 83508.0 44 86048.5 45 90792.5 46 94500.5 47 95722.5 48 97182.5 49 97300.5 50 96039.5 51 94121.5 52 94377.5 53 95827.5 54 94746.5 55 88720.0 56 80434.0 57 71858.0 58 63186.5 59 55284.5 60 46651.0 61 38498.0 62 31277.0 63 23861.0 64 17449.5 65 13028.5 66 9513.5 67 7302.5 68 5575.5 69 3915.5 70 2934.0 71 2175.5 72 1554.0 73 1167.5 74 851.5 75 626.5 76 439.5 77 262.0 78 157.5 79 94.5 80 56.5 81 39.0 82 23.5 83 13.0 84 7.0 85 3.0 86 1.5 87 0.5 88 1.5 89 2.5 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018678988458591613 2 0.0030434197081208327 3 0.00372818914244802 4 0.0329069755940565 5 0.0023586502737936456 6 3.804274635151041E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.804274635151041E-5 24-25 5.896625684484114E-4 26-27 0.0019782228102785413 28-29 7.608549270302083E-5 30-31 0.0058205401917810935 32-33 0.020980574612857992 34-35 0.012592149042349945 36-37 6.46726687975677E-4 38-39 5.135770757453905E-4 40-41 0.00186409457122401 42-43 0.007399314165368774 44-45 0.00701888670185367 46-47 0.006961822582326405 48-49 0.008768853034023149 50-51 0.19314302322661836 52-53 0.04751539019303651 54-55 0.016548594662907028 56-57 0.009187323243889765 58-59 0.018431710607306796 60-61 0.05147183581359359 62-63 0.09369928426377015 64-65 0.0975796443916242 66-67 0.10300073574671444 68-69 0.10028067938258145 70-71 0.08890589822347983 72-73 0.06716446868359163 74-75 0.24784849248009033 76-77 0.29964369163767174 78-79 0.006200967655296197 80-81 6.847694343271874E-4 82-83 0.0 84-85 1.9021373175755206E-5 86-87 0.0 88-89 2.2825647810906244E-4 90-91 0.0056113050868477855 92-93 0.017081193111828173 94-95 0.0014646457345331508 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2628622.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.64820820138536 #Duplication Level Percentage of deduplicated Percentage of total 1 75.45934601818755 40.48258705924105 2 13.281741530335436 14.250832697928443 3 4.557523062735249 7.335088384567083 4 2.064075078750764 4.429357182724475 5 1.17300837606415 3.146489879052923 6 0.71624810057285 2.3055256334027434 7 0.5023330267041861 1.8864486762140773 8 0.3677471499854455 1.5783180534308217 9 0.27483783735412365 1.3270101767993263 >10 1.4664076188760626 14.043270765483392 >50 0.09473861440620787 3.4838331728064484 >100 0.03836585711493364 3.712558998144765 >500 0.0023473540026878862 0.8509523840060031 >1k 0.0012803749105380318 1.1677269361983662 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4923 0.18728444028848573 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4370 0.1662468015561005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2659 0.10115566254866619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.804274635151041E-5 0.0 5 0.0 0.0 0.0 3.804274635151041E-5 3.804274635151041E-5 6 0.0 0.0 0.0 3.804274635151041E-5 7.608549270302083E-5 7 0.0 0.0 0.0 3.804274635151041E-5 7.608549270302083E-5 8 0.0 0.0 0.0 3.804274635151041E-5 7.608549270302083E-5 9 0.0 0.0 0.0 3.804274635151041E-5 7.608549270302083E-5 10-11 0.0 0.0 0.0 3.804274635151041E-5 7.608549270302083E-5 12-13 0.0 0.0 0.0 3.804274635151041E-5 2.8532059763632805E-4 14-15 0.0 0.0 0.0 3.804274635151041E-5 4.565129562181249E-4 16-17 0.0 0.0 0.0 3.804274635151041E-5 6.086839416241666E-4 18-19 0.0 0.0 0.0 5.7064119527265616E-5 6.46726687975677E-4 20-21 0.0 0.0 0.0 7.608549270302083E-5 6.657480611514322E-4 22-23 0.0 0.0 0.0 1.5217098540604165E-4 7.228121806786978E-4 24-25 0.0 0.0 0.0 3.8042746351510414E-4 7.608549270302082E-4 26-27 0.0 0.0 0.0 9.700900319635156E-4 7.798763002059635E-4 28-29 0.0 0.0 0.0 0.002624949498254218 7.988976733817187E-4 30-31 0.0 0.0 0.0 0.00844548969003531 7.988976733817187E-4 32-33 0.0 0.0 0.0 0.023187053901245598 8.369404197332291E-4 34-35 0.0 0.0 0.0 0.04511869717289135 8.369404197332291E-4 36-37 0.0 0.0 0.0 0.07918597653066892 8.749831660847395E-4 38-39 0.0 0.0 0.0 0.12966870093912325 9.130259124362499E-4 40-41 0.0 0.0 0.0 0.18279539621900753 9.130259124362499E-4 42-43 0.0 0.0 0.0 0.23866116923620057 9.130259124362499E-4 44-45 0.0 0.0 0.0 0.3065484501004709 9.32047285612005E-4 46-47 0.0 0.0 0.0 0.3856202983920853 9.891114051392706E-4 48-49 0.0 0.0 0.0 0.4680208869894568 0.0010651968978422914 50-51 0.0 0.0 0.0 0.5533507670558947 0.0011032396441938018 52-53 0.0 0.0 0.0 0.6376534929708417 0.0012744320027755988 54-55 0.0 0.0 0.0 0.7293174903048061 0.0013314961223028644 56-57 0.0 0.0 0.0 0.8383289799750592 0.0013314961223028644 58-59 0.0 0.0 0.0 0.9571745195771777 0.0013314961223028644 60-61 0.0 0.0 0.0 1.0866339854113676 0.0013505174954786196 62-63 0.0 0.0 0.0 1.220582495315036 0.0014075816150058852 64-65 0.0 0.0 0.0 1.360047203439673 0.0014456243613573956 66-67 0.0 0.0 0.0 1.5166311474224898 0.0014646457345331508 68-69 0.0 0.0 0.0 1.6927500416568073 0.001483667107708906 70-71 0.0 0.0 0.0 1.8843523336561896 0.0015407312272361718 72-73 0.0 0.0 0.0 2.089669035715291 0.0018070304516967445 74-75 0.0 0.0 0.0 2.3043442533768643 0.00186409457122401 76-77 0.0 0.0 0.0 2.5365952198528356 0.0019021373175755205 78-79 0.0 0.0 0.0 2.7996798322467056 0.0019211586907512757 80-81 0.0 0.0 0.0 3.081405390352816 0.0019782228102785413 82-83 0.0 0.0 0.0 3.384244672684015 0.002130393795684583 84-85 0.0 0.0 0.0 3.6999994674015513 0.002130393795684583 86-87 0.0 0.0 0.0 4.041813543369872 0.002149415168860338 88-89 0.0 0.0 0.0 4.408697789183838 0.0021684365420360932 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3035 0.0 54.603687 1 GTATCAA 5850 0.0 43.101646 1 CAACGCA 7340 0.0 33.62131 5 ATCAACG 7390 0.0 33.408447 3 TCAACGC 7500 0.0 33.170414 4 TATCAAC 7730 0.0 32.30875 2 AACGCAG 8010 0.0 30.986786 6 ACGCAGA 9200 0.0 26.72079 7 CGCAGAG 9440 0.0 25.840355 8 GTACATG 8405 0.0 24.74524 1 TACATGG 8320 0.0 24.42499 2 ACATGGG 8035 0.0 24.226763 3 GCAGAGT 10605 0.0 22.64369 9 TCTAACG 255 4.9294613E-10 22.343695 2 GTGGTAT 1450 0.0 21.941286 1 CATGGGG 5805 0.0 19.628626 4 AGAGTAC 10090 0.0 19.048956 10-11 GAGTACA 5835 0.0 18.717258 1 CTAACGC 255 2.6908128E-7 18.619038 3 CAGAGTA 10330 0.0 18.583416 10-11 >>END_MODULE