FastQCFastQC Report
Sat 18 Jun 2016
SRR1930916_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930916_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718844
Sequences flagged as poor quality0
Sequence length101
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30960.1801210581064948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12700.049.16881
GTATCAA20900.041.20231
ATCAACG26400.033.3684923
TCAACGC27050.032.5565154
CAACGCA28300.031.088625
GTACATG40350.030.7017561
TATCAAC28050.030.513092
TACATGG40600.029.1415022
AACGCAG31800.028.3281086
CTAGTAC4250.027.242153
ACATGGG45100.026.3413433
ACGCAGA36350.024.2908927
CGCAGAG39200.022.9069568
CATGGGG38250.022.76054
TAGTACT5150.022.4743824
GTACTAG3850.020.9281521
GCAGAGT39800.020.7169539
GAGTACA30200.020.1766971
AGTACAT28050.019.7437652
GTATATG4350.019.680281
Produced by FastQC (version 0.11.5)