FastQCFastQC Report
Sat 18 Jun 2016
SRR1930916_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930916_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718844
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19650.044.465441
GTATCAA31050.039.9159241
GTGGTAT8750.032.0192571
CAACGCA39250.031.558435
TCAACGC40250.030.7824274
TATCAAC41100.030.494092
ATCAACG40350.030.472623
AACGCAG43150.028.8135746
GTACTAG4250.027.9330521
TGGTATC10050.026.4530352
ACGCAGA50500.024.150077
TATACCG2052.6961061E-823.1505175
GTACATG59900.022.5935941
CGCAGAG54500.022.2905148
TACATGG62200.021.2181722
TAGTACT7200.021.0982174
GTATTAT3252.1827873E-1120.4555871
CTAGTAC7200.020.4400883
ATTATAC3302.7284841E-1120.1403793
ACATGGG64300.019.786883
Produced by FastQC (version 0.11.5)