##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930916_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1718844 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.193830853759852 31.0 31.0 33.0 30.0 34.0 2 31.298484330166087 31.0 31.0 34.0 30.0 34.0 3 31.3859390380977 31.0 31.0 34.0 28.0 34.0 4 35.25693489345164 35.0 35.0 37.0 33.0 37.0 5 35.02117120576387 35.0 35.0 37.0 32.0 37.0 6 35.06900277163024 36.0 35.0 37.0 32.0 37.0 7 34.927046898962324 36.0 35.0 37.0 32.0 37.0 8 34.902618853136175 36.0 35.0 37.0 32.0 37.0 9 36.39872379343326 38.0 35.0 39.0 32.0 39.0 10-11 36.316995317783345 38.0 35.0 39.0 32.0 39.0 12-13 36.192548596614934 38.0 35.0 39.0 31.5 39.0 14-15 37.207601446088184 39.0 36.0 40.0 31.5 41.0 16-17 37.14456547540091 39.0 36.0 40.0 31.0 41.0 18-19 37.088666568926556 39.0 36.0 40.0 31.0 41.0 20-21 37.027333777818114 39.0 36.0 40.0 31.0 41.0 22-23 36.87814659154641 39.0 36.0 40.0 31.0 41.0 24-25 36.73276021558675 38.0 35.0 40.0 30.0 41.0 26-27 36.42801586415055 38.0 35.0 40.0 30.0 41.0 28-29 36.22066633155772 38.0 35.0 40.0 30.0 41.0 30-31 35.97302692972719 38.0 34.0 40.0 29.0 41.0 32-33 35.73600600170813 38.0 34.0 40.0 28.5 41.0 34-35 36.00893623854172 38.0 34.5 40.0 30.0 41.0 36-37 36.087112617549934 38.0 35.0 40.0 30.0 41.0 38-39 36.04054236451941 38.0 35.0 40.0 29.0 41.0 40-41 35.928774222675244 38.0 34.5 40.0 29.0 41.0 42-43 35.76763016306309 38.0 34.0 40.0 28.5 41.0 44-45 35.5705116345637 38.0 34.0 40.0 28.0 41.0 46-47 35.334354368401094 38.0 34.0 40.0 27.0 41.0 48-49 35.10657773480315 38.0 33.0 40.0 27.0 41.0 50-51 34.78598843175995 37.5 33.0 40.0 26.0 41.0 52-53 34.50658204002225 37.0 33.0 40.0 26.0 41.0 54-55 34.260444810581994 37.0 33.0 40.0 26.0 41.0 56-57 33.92978449469527 36.5 32.0 39.0 25.0 41.0 58-59 33.60574519851714 36.0 32.0 39.0 24.5 41.0 60-61 33.21206200213632 35.5 31.0 39.0 23.5 40.0 62-63 32.82532213510941 35.0 31.0 38.5 23.0 40.0 64-65 32.4466400092155 35.0 31.0 38.0 22.0 40.0 66-67 32.05523479734054 35.0 31.0 37.5 22.0 40.0 68-69 31.617128430503293 34.0 30.0 37.0 21.0 39.0 70-71 31.177609195482546 34.0 30.0 36.0 20.0 39.0 72-73 30.733792886381778 34.0 29.5 36.0 19.5 38.5 74-75 30.201859505574678 34.0 29.0 35.0 18.0 37.0 76-77 28.896148806988883 32.5 27.0 34.5 16.0 36.0 78-79 29.664434061497147 33.0 29.0 35.0 18.0 36.0 80-81 29.7061152728229 34.0 29.0 35.0 17.5 36.0 82-83 29.476933043371012 34.0 29.0 35.0 16.0 35.5 84-85 29.211504069013827 33.0 29.0 35.0 12.0 35.0 86-87 28.906806260486697 33.0 29.0 35.0 7.5 35.0 88-89 28.59366702271992 33.0 28.5 35.0 6.0 35.0 90-91 28.26709288335649 33.0 27.0 35.0 2.0 35.0 92-93 27.9027980433361 33.0 27.0 35.0 2.0 35.0 94-95 27.457769873240387 33.0 26.5 35.0 2.0 35.0 96-97 26.948262611383 33.0 25.0 34.5 2.0 35.0 98-99 26.39803641284491 32.0 25.0 34.0 2.0 35.0 100-101 25.117572915284924 31.5 21.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5998428211301459 1101 2 0.6899279086343846 1101 3 0.6220184293465643 1101 4 0.3706339718501894 1101 5 0.4705380975382596 1101 6 0.4909717396196598 1101 7 0.5300378856622672 1101 8 0.6095676617056043 1101 9 0.7954587100903368 1101 10-11 0.7269119104147421 1101 12-13 0.767263695194373 1101 14-15 1.0043476357692285 1101 16-17 1.0424131675023887 1101 18-19 0.9692031319240968 1101 20-21 0.9864087962747234 1101 22-23 1.068450977572759 1101 24-25 1.0193366906666057 1101 26-27 1.0586566544838618 1101 28-29 1.122336787409182 1101 30-31 1.0903130298022248 1101 32-33 0.8782965134633685 1101 34-35 0.9015026117118907 1101 36-37 0.9629993861333901 1101 38-39 0.9702689231079873 1101 40-41 1.036932233629706 1101 42-43 0.8905863000224556 1101 44-45 0.9404695846571727 1101 46-47 1.001643255229645 1101 48-49 0.9778672228022245 1101 50-51 1.0756621471809709 1101 52-53 0.9179840050064954 1101 54-55 0.8603210651415694 1101 56-57 0.985421875332186 1101 58-59 0.981996251561128 1101 60-61 0.8020778723499404 1101 62-63 0.8296898563010018 1101 64-65 0.9716982286408822 1101 66-67 1.0506964819061437 1101 68-69 0.9145357909236047 1101 70-71 0.6485115881557206 1101 72-73 0.8310619135397808 1101 74-75 0.8096549178950134 1101 76-77 1.072683536118351 1101 78-79 1.135685175789117 1101 80-81 0.9710080628158302 1101 82-83 1.133797253093629 1101 84-85 1.1311207729619213 1101 86-87 1.168496760363329 1101 88-89 1.0029882429159294 1101 90-91 1.0423538285006693 1101 92-93 1.1244816192788605 1101 94-95 0.9976692759606962 1101 96-97 1.1700315813711342 1101 98-99 1.2452183868676023 1101 100-101 1.5154087564498724 1102 1 0.5229435734909522 1102 2 0.5228412182855777 1102 3 0.4397594679244179 1102 4 0.3072927077926195 1102 5 0.5596628909119943 1102 6 0.5114515442039007 1102 7 0.5428576009179551 1102 8 0.5633493983564648 1102 9 0.7295166847848122 1102 10-11 0.7129206782239521 1102 12-13 0.5947453686195061 1102 14-15 0.8518279380339209 1102 16-17 0.7472981804649308 1102 18-19 0.8164038063318841 1102 20-21 0.7217646758868383 1102 22-23 0.7950895142906589 1102 24-25 0.7809007784513398 1102 26-27 0.671468244344382 1102 28-29 0.6807650439786883 1102 30-31 0.7558400251580508 1102 32-33 0.9116899831848428 1102 34-35 0.8661872955959282 1102 36-37 0.8849381435065524 1102 38-39 0.8605126087872321 1102 40-41 0.8127735294271048 1102 42-43 0.8662478849315747 1102 44-45 0.9960545877168911 1102 46-47 0.8615890563047301 1102 48-49 0.8327892444005442 1102 50-51 0.8909319229962378 1102 52-53 0.8462872320579606 1102 54-55 0.9975320105566894 1102 56-57 0.8959523874606035 1102 58-59 1.113502915654756 1102 60-61 0.9815913389228257 1102 62-63 0.8886459461577161 1102 64-65 0.9344439266250149 1102 66-67 0.868415302606607 1102 68-69 0.7662330463174634 1102 70-71 0.697583579156845 1102 72-73 0.7122207091530832 1102 74-75 0.8328259422280375 1102 76-77 1.1707262680793384 1102 78-79 1.2574515224337084 1102 80-81 1.1053751199246626 1102 82-83 1.1666254365345807 1102 84-85 1.3005301836930272 1102 86-87 1.3020014464310705 1102 88-89 1.2709082306262722 1102 90-91 1.2962533833813659 1102 92-93 1.3602529731982216 1102 94-95 1.4055457039354167 1102 96-97 1.4229900504451187 1102 98-99 1.5580867275349526 1102 100-101 1.6205554960081088 1103 1 0.38304976297764526 1103 2 0.4898395090883021 1103 3 0.4938670227756994 1103 4 0.2286347515540541 1103 5 0.24614603065257512 1103 6 0.3300200042819412 1103 7 0.32176321635355265 1103 8 0.27978086353219567 1103 9 0.4287253712941137 1103 10-11 0.4743313141151049 1103 12-13 0.5173450178105057 1103 14-15 0.7057831351397539 1103 16-17 0.6527965136238549 1103 18-19 0.6392595859600547 1103 20-21 0.7025860373646182 1103 22-23 0.6011479459152937 1103 24-25 0.6832703587263111 1103 26-27 0.6842386388550139 1103 28-29 0.7525625414746955 1103 30-31 0.7485479068995389 1103 32-33 0.8474935550583353 1103 34-35 0.6969107395038634 1103 36-37 0.6324285386151516 1103 38-39 0.4928589180672276 1103 40-41 0.7012178858370888 1103 42-43 0.6102644486975848 1103 44-45 0.6264624779296213 1103 46-47 0.5202217483153575 1103 48-49 0.7637997138567343 1103 50-51 0.5490457515482987 1103 52-53 0.40865611470138674 1103 54-55 0.6433706384151066 1103 56-57 0.5516799424115462 1103 58-59 0.5369067097737172 1103 60-61 0.5589462191046053 1103 62-63 0.5520972788831173 1103 64-65 0.4208793533512818 1103 66-67 0.5002988081054411 1103 68-69 0.48011192751579657 1103 70-71 0.3874290537705285 1103 72-73 0.4740848055458322 1103 74-75 0.574370897106224 1103 76-77 0.6041516638606588 1103 78-79 0.7071367162008713 1103 80-81 0.7308179913760817 1103 82-83 0.6333527722298982 1103 84-85 0.8058284460921818 1103 86-87 0.6887883050460708 1103 88-89 0.6151439254423714 1103 90-91 0.7952106733818134 1103 92-93 0.677116596198136 1103 94-95 0.7583628895901171 1103 96-97 0.7808445095564345 1103 98-99 0.8508390832871733 1103 100-101 1.0716191349538846 1104 1 0.438585672448486 1104 2 0.5205552695346611 1104 3 0.47343688068092504 1104 4 0.3503440323644682 1104 5 0.45322290756806893 1104 6 0.5375326670765403 1104 7 0.4893639650479784 1104 8 0.49884363813087873 1104 9 0.5594414889420136 1104 10-11 0.6472847278902236 1104 12-13 0.5976653561518006 1104 14-15 0.7742265222676394 1104 16-17 0.8202561677098856 1104 18-19 0.828946998892178 1104 20-21 0.7371417143686969 1104 22-23 0.4926810192059037 1104 24-25 0.7165356451662248 1104 26-27 1.0806305460969199 1104 28-29 0.6491571605906898 1104 30-31 0.769938028133744 1104 32-33 0.7933301925059126 1104 34-35 0.866351969134449 1104 36-37 0.8541953288735584 1104 38-39 0.8134999876715128 1104 40-41 0.8752009330519925 1104 42-43 0.8406674791954529 1104 44-45 1.0119597381938021 1104 46-47 0.963022172670783 1104 48-49 0.9693218710682814 1104 50-51 1.0075201794900366 1104 52-53 1.0390328908860411 1104 54-55 0.9198794577211302 1104 56-57 0.909013647234481 1104 58-59 0.8669957601177032 1104 60-61 0.9516250163355764 1104 62-63 0.7704724405168051 1104 64-65 0.8589790054369857 1104 66-67 0.8558179291038925 1104 68-69 0.9201297518726541 1104 70-71 0.8959384874632086 1104 72-73 0.841991675960255 1104 74-75 0.9322287951894808 1104 76-77 1.1775369342979438 1104 78-79 0.8938191711756147 1104 80-81 0.82488433799017 1104 82-83 0.9989503346865867 1104 84-85 1.1849050086703201 1104 86-87 1.1721516433087835 1104 88-89 1.2923337494848894 1104 90-91 1.0365860421924076 1104 92-93 1.0022414381364548 1104 94-95 1.17840153879677 1104 96-97 1.132057565987136 1104 98-99 1.090453448504313 1104 100-101 1.403819246400765 1105 1 0.29843868630216974 1105 2 0.3448999164437545 1105 3 0.38539929984120036 1105 4 0.13270250638682057 1105 5 0.20183461650786683 1105 6 0.3416175640640162 1105 7 0.44400338135461936 1105 8 0.3663674582827525 1105 9 0.5089794337929803 1105 10-11 0.589421553605888 1105 12-13 0.5555250763148365 1105 14-15 0.5858546887669149 1105 16-17 0.6063836194717567 1105 18-19 0.549412933285133 1105 20-21 0.5255913984494569 1105 22-23 0.5357406267114442 1105 24-25 0.5170481836265353 1105 26-27 0.5484208649920319 1105 28-29 0.6414292284402592 1105 30-31 0.7952593942652797 1105 32-33 0.8657086472914628 1105 34-35 0.7710846467161403 1105 36-37 0.7895275356319402 1105 38-39 0.5920004739055216 1105 40-41 0.5942440351717622 1105 42-43 0.4865775338768472 1105 44-45 0.4716833553496329 1105 46-47 0.5137419584089855 1105 48-49 0.596123992430158 1105 50-51 0.6169188435422015 1105 52-53 0.5134763788045902 1105 54-55 0.49841156245009444 1105 56-57 0.4339218473184374 1105 58-59 0.42461980292755186 1105 60-61 0.5840706404952982 1105 62-63 0.45808436733195634 1105 64-65 0.3401726434769543 1105 66-67 0.3946674941503119 1105 68-69 0.609284076698227 1105 70-71 0.38060218889959074 1105 72-73 0.4436092675343275 1105 74-75 0.6495660927912574 1105 76-77 0.6086391709743388 1105 78-79 0.7536628854719609 1105 80-81 0.5159065453330989 1105 82-83 0.590565588086239 1105 84-85 0.8191813171180371 1105 86-87 0.8802332243398148 1105 88-89 0.9205254904151658 1105 90-91 0.7015774600294868 1105 92-93 0.7299318000752244 1105 94-95 0.6202660203331618 1105 96-97 0.35089474933720766 1105 98-99 0.6828910484745556 1105 100-101 1.1414641202190587 1106 1 0.05051688827261458 1106 2 0.15598879164900836 1106 3 0.10290066820464006 1106 4 0.01186746259919147 1106 5 0.053469810815478525 1106 6 0.03312002711820128 1106 7 0.07620439394356282 1106 8 0.17028097771351725 1106 9 0.17586845951821317 1106 10-11 0.15245179443785162 1106 12-13 0.07148361490268229 1106 14-15 0.1454811255485211 1106 16-17 0.10478862084011098 1106 18-19 0.09845165797040778 1106 20-21 0.053133988761437934 1106 22-23 0.2021550013693556 1106 24-25 0.2206350062866349 1106 26-27 0.22421280533138344 1106 28-29 0.2869632322716811 1106 30-31 0.29285620597847384 1106 32-33 0.29976635801943274 1106 34-35 0.23691404600459265 1106 36-37 0.25002459365055785 1106 38-39 0.3034297295158055 1106 40-41 0.36302054721335963 1106 42-43 0.17326934422167994 1106 44-45 0.12368287642242848 1106 46-47 0.050828883696347305 1106 48-49 0.11095790731795319 1106 50-51 0.1222383564048144 1106 52-53 0.05839718477065503 1106 54-55 0.2898515939224495 1106 56-57 0.46926838535894433 1106 58-59 0.5103434961404716 1106 60-61 0.27337516977827647 1106 62-63 0.2797908875837365 1106 64-65 0.29022737801444265 1106 66-67 0.12317991204849932 1106 68-69 0.15526964046395975 1106 70-71 0.2635857343292578 1106 72-73 0.278597465399681 1106 74-75 0.2553619671754923 1106 76-77 0.3797889383525508 1106 78-79 0.5866498518102183 1106 80-81 0.4177863346051325 1106 82-83 0.43351167456680884 1106 84-85 0.3603690565816393 1106 86-87 0.4325517451389409 1106 88-89 0.46376149889901797 1106 90-91 0.3972406031603022 1106 92-93 0.45841000888748695 1106 94-95 0.5521728347830823 1106 96-97 0.578894030946401 1106 98-99 0.45764559828298346 1106 100-101 0.8224344609415546 1107 1 0.18344768417539825 1107 2 0.1504125528509057 1107 3 0.09184646283500086 1107 4 0.006607058240220454 1107 5 0.10781037577284991 1107 6 0.1566249259602941 1107 7 0.1500284219005934 1107 8 0.016785440295087994 1107 9 0.2320307529761223 1107 10-11 0.17598089210567736 1107 12-13 0.025311005701219358 1107 14-15 0.11320579971643951 1107 16-17 0.09831784915887454 1107 18-19 0.22605501990303623 1107 20-21 0.37122485478401046 1107 22-23 0.22705096346012965 1107 24-25 0.22646232210290407 1107 26-27 0.22362288794821694 1107 28-29 0.27502088455386087 1107 30-31 0.17611987774697013 1107 32-33 0.28717771419887583 1107 34-35 0.3242639962436229 1107 36-37 0.42321007935392885 1107 38-39 0.4190120691446211 1107 40-41 0.34750134606999694 1107 42-43 0.3153089246390337 1107 44-45 0.41151171915050355 1107 46-47 0.29280024160265583 1107 48-49 0.26918712700238956 1107 50-51 0.23681070748546773 1107 52-53 0.19728949772395765 1107 54-55 0.22581942766174734 1107 56-57 0.2074612329586003 1107 58-59 0.16438535329241688 1107 60-61 0.08597827040903638 1107 62-63 -0.03575143309502238 1107 64-65 -0.0932956074617124 1107 66-67 0.07184985710694036 1107 68-69 0.09001489385861206 1107 70-71 -0.07119607292539243 1107 72-73 -0.05069813859392269 1107 74-75 0.2531597888267392 1107 76-77 0.152979195655373 1107 78-79 0.2431683962906135 1107 80-81 0.24813751296297326 1107 82-83 0.2577725310916321 1107 84-85 0.31746556104422297 1107 86-87 0.4116116662030542 1107 88-89 0.5615234767497093 1107 90-91 0.4854840921992576 1107 92-93 0.5184252268814156 1107 94-95 0.7761715941672307 1107 96-97 0.7930023723838815 1107 98-99 0.796139028244994 1107 100-101 1.0020910023499496 1108 1 0.2705730099257302 1108 2 0.4671000875510849 1108 3 0.33695996240001236 1108 4 0.23158063771067816 1108 5 0.3027323037042109 1108 6 0.27895190761223176 1108 7 0.2816460035671682 1108 8 0.34761760211020487 1108 9 0.3865334087758896 1108 10-11 0.44272163257626573 1108 12-13 0.45720958341124884 1108 14-15 0.6124951737399584 1108 16-17 0.6966015176946456 1108 18-19 0.596912986446668 1108 20-21 0.5593986414105032 1108 22-23 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-1.0629062311575694 2316 86-87 -0.7949749109409154 2316 88-89 -0.7899366463030297 2316 90-91 -0.3052951704044453 2316 92-93 -0.4379818003086804 2316 94-95 -0.3009430477320194 2316 96-97 -0.19554172596278363 2316 98-99 -0.14840277607663666 2316 100-101 -0.2678306137787452 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 38.0 5 61.0 6 158.0 7 423.0 8 897.0 9 1583.0 10 2329.0 11 3414.0 12 4519.0 13 5822.0 14 6990.0 15 8458.0 16 9820.0 17 11200.0 18 11125.0 19 10841.0 20 11567.0 21 12057.0 22 13646.0 23 16256.0 24 19690.0 25 25103.0 26 31725.0 27 39719.0 28 49371.0 29 60123.0 30 72034.0 31 84807.0 32 101604.0 33 121449.0 34 147964.0 35 183139.0 36 235450.0 37 267204.0 38 140345.0 39 7898.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.237281261437715 17.677724263578476 13.223321794515948 25.861672680467862 2 17.681309845601405 19.23181199885036 36.275583285528604 26.81129487001963 3 21.071782227181806 22.059982010516748 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25.63552179125092 23.912997314764258 25.254858862950584 25.196622031034238 24-25 25.48142304401844 24.17623782134019 25.260866871281824 25.081472263359544 26-27 25.38503248747975 24.349174082624316 25.024464515108075 25.241328914787854 28-29 25.282086800906306 24.473518937028224 25.140916134501058 25.10347812756441 30-31 25.52225532495483 24.354877326108593 25.000123638269912 25.12274371066667 32-33 25.46624729771801 24.349371964933617 24.869955861515137 25.314424875833236 34-35 25.429360108553222 24.42307826584833 25.142208443597248 25.005353182001205 36-37 25.5215893574303 24.2775767046595 24.94265004220887 25.258183895701325 38-39 25.37275355910707 24.368756654235728 24.99901095512645 25.259478831530753 40-41 25.54994020539324 24.12327666881642 25.130548891508937 25.196234234281405 42-43 25.424515279175395 24.155675045405076 25.003003731609237 25.41680594381029 44-45 25.3836108248 24.192566306598323 25.01615318308619 25.407669685515486 46-47 25.4799455754765 24.27866705882113 24.812788424810346 25.428598940892023 48-49 25.56245363434041 24.375841854917304 24.75069457576331 25.31100993497898 50-51 25.415666160232387 24.34169129619877 24.851924076243183 25.390718467325662 52-53 25.555869869408415 24.251205451831982 24.772791828771126 25.420132849988477 54-55 25.322788717625844 24.17314940190476 24.87115509162143 25.63290678884797 56-57 25.386965379744648 24.287970609686667 24.743354057794395 25.581709952774297 58-59 25.355302072589364 24.328477846264406 24.8676973120493 25.448522769096932 60-61 25.42545237375256 24.346763657360988 24.811237478284635 25.41654649060182 62-63 25.357301434097185 24.401896601170744 24.634094856262728 25.60670710846934 64-65 25.150826224895294 24.351476349982065 24.782466655175146 25.715230769947496 66-67 25.186385718561933 24.44445350344585 24.676787771279884 25.692373006712334 68-69 25.150290272930516 24.310277857204003 24.813283662662613 25.72614820720287 70-71 24.98416891844089 24.244249641671658 24.836324813324254 25.935256626563202 72-73 25.204940580185166 24.28673226240079 24.69838906021041 25.809938097203634 74-75 25.018415427549947 24.414366074787342 24.664804224904422 25.90241427275829 76-77 25.16684240285737 24.212381903237535 24.560917243717405 26.05985845018769 78-79 24.995243533969894 24.324683644098812 24.57571450265402 26.10435831927727 80-81 25.06368440113973 24.503244217351654 24.569132155495534 25.863939226013084 82-83 25.142159497895094 24.30569615392671 24.780637451682644 25.77150689649555 84-85 25.106881458375934 24.3568422605083 24.43087459678557 26.105401684330193 86-87 24.881868278913036 24.479010311581504 24.693834926264397 25.945286483241066 88-89 24.928774763023732 24.333548788341925 24.550380692128368 26.187295756505975 90-91 24.835852730881097 24.42592819935173 24.536154872402953 26.202064197364216 92-93 24.976746039989536 24.293369108208815 24.576111658576878 26.153773193224772 94-95 24.870543039984828 24.128781785352636 24.770241349488266 26.23043382517427 96-97 24.73298914852075 24.16612560534871 24.74479941169763 26.356085834432907 98-99 24.5581914356393 24.166474677166747 24.65668786696175 26.6186460202322 100-101 24.582102855174757 24.237074452364496 24.623467865612003 26.557354826848744 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 70.0 1 49.5 2 42.5 3 58.0 4 50.5 5 54.5 6 101.0 7 207.5 8 319.0 9 374.0 10 358.0 11 317.0 12 315.0 13 352.5 14 396.0 15 447.5 16 509.0 17 589.5 18 686.0 19 760.5 20 852.5 21 964.5 22 1134.0 23 1302.5 24 1515.0 25 1788.5 26 2082.0 27 2471.5 28 2932.0 29 3354.0 30 3866.0 31 4567.5 32 5344.0 33 5772.0 34 6113.5 35 6793.0 36 7838.5 37 9354.5 38 11079.5 39 13653.5 40 17591.5 41 23316.0 42 30334.0 43 38762.0 44 48419.5 45 58389.0 46 68245.0 47 76587.0 48 83779.5 49 89210.5 50 92074.0 51 94309.0 52 96552.5 53 97640.0 54 96847.0 55 91957.5 56 84034.5 57 75478.0 58 67760.5 59 60327.0 60 51213.5 61 41994.0 62 33707.0 63 26120.0 64 19565.5 65 14418.5 66 10597.5 67 8280.0 68 6334.0 69 4603.0 70 3438.5 71 2429.0 72 1700.0 73 1274.5 74 944.0 75 685.0 76 492.0 77 287.5 78 153.0 79 80.5 80 44.5 81 26.5 82 14.5 83 7.5 84 6.5 85 5.0 86 2.0 87 2.0 88 2.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017279054992774212 2 0.0026762172716081273 3 0.0036070754530370414 4 0.032405500440994064 5 0.0022107881808936707 6 1.7453590901792134E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.9089318169653556E-5 24-25 4.3633977254480335E-4 26-27 0.0016290018175005993 28-29 1.454465908482678E-4 30-31 0.007010525678886507 32-33 0.02263148953599047 34-35 0.013962872721433707 36-37 6.399649997323783E-4 38-39 8.145009087502996E-4 40-41 0.0025307706807598594 42-43 0.008639527496387106 44-45 0.008290455678351264 46-47 0.0077668479512974994 48-49 0.009832189541342903 50-51 0.189342371966275 52-53 0.0474737672528746 54-55 0.017744484083488672 56-57 0.01061760113192355 58-59 0.01986800430987338 60-61 0.0511099320240813 62-63 0.09084594064382806 64-65 0.09518024905110645 66-67 0.09925275359485794 68-69 0.09698378677762495 70-71 0.08694797200909449 72-73 0.06696361042654249 74-75 0.2472010258057159 76-77 0.2967692239668056 78-79 0.007621401360449233 80-81 9.599474995985674E-4 82-83 0.0 84-85 2.9089318169653556E-5 86-87 0.0 88-89 5.817863633930711E-5 90-91 0.006574185906341704 92-93 0.018588074310408625 94-95 0.0017162697720095599 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1718844.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.70404956987716 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35835240049414 45.41248553962633 2 12.519132335185112 14.698475303531175 3 4.1588616011363735 7.324260527621049 4 1.8798728026728482 4.41424584772683 5 1.0167160485702234 2.984267465687801 6 0.6692851353160815 2.3573848655986307 7 0.4552719552554456 1.8708415200363406 8 0.324202686493364 1.5225608462875027 9 0.24408341874084633 1.289581660165295 >10 1.2874314874700488 13.212439988575364 >50 0.06710327888929224 2.629507532899365 >100 0.018891572169644145 1.853678906951752 >500 4.970485040868604E-4 0.1853434836632271 >1k 2.982291024521163E-4 0.24492651162927082 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.3271454535722847E-4 2 0.0 0.0 0.0 0.0 2.3271454535722847E-4 3 0.0 0.0 0.0 0.0 2.3271454535722847E-4 4 0.0 0.0 0.0 0.0 2.3271454535722847E-4 5 0.0 0.0 0.0 0.0 2.9089318169653555E-4 6 0.0 0.0 0.0 0.0 2.9089318169653555E-4 7 0.0 0.0 0.0 0.0 2.9089318169653555E-4 8 0.0 0.0 0.0 0.0 2.9089318169653555E-4 9 0.0 0.0 0.0 0.0 2.9089318169653555E-4 10-11 0.0 0.0 0.0 0.0 2.9089318169653555E-4 12-13 0.0 0.0 0.0 2.908931816965356E-5 9.308581814289139E-4 14-15 0.0 0.0 0.0 5.817863633930712E-5 0.0012217513631254495 16-17 0.0 0.0 0.0 5.817863633930712E-5 0.0015417338629916386 18-19 0.0 0.0 0.0 5.817863633930712E-5 0.0018617163628578278 20-21 0.0 0.0 0.0 8.726795450896067E-5 0.002094430908215056 22-23 0.0 0.0 0.0 1.7453590901792134E-4 0.0022107881808936703 24-25 0.0 0.0 0.0 4.363397725448034E-4 0.0022689668172329773 26-27 0.0 0.0 0.0 0.0010763047722771816 0.0025889493170991665 28-29 0.0 0.0 0.0 0.002850753180626049 0.0027053065897777806 30-31 0.0 0.0 0.0 0.010152172041209093 0.002763485226117088 32-33 0.0 0.0 0.0 0.02993290839657351 0.0029089318169653556 34-35 0.0 0.0 0.0 0.06047669247470975 0.00302528908964397 36-37 0.0 0.0 0.0 0.10902676449986154 0.00302528908964397 38-39 0.0 0.0 0.0 0.17581583901738612 0.0031416463623225844 40-41 0.0 0.0 0.0 0.24376848626169684 0.0031416463623225844 42-43 0.0 0.0 0.0 0.3169281214583755 0.0031998249986618914 44-45 0.0 0.0 0.0 0.40588325642117606 0.0032870929531708523 46-47 0.0 0.0 0.0 0.5116229279678668 0.0034325395440191198 48-49 0.0 0.0 0.0 0.6234131776938454 0.003752522043885309 50-51 0.0 0.0 0.0 0.7336035149204931 0.003897968634733577 52-53 0.0 0.0 0.0 0.8489426614631694 0.0041306831800908056 54-55 0.0 0.0 0.0 0.9757429993646893 0.0041306831800908056 56-57 0.0 0.0 0.0 1.1211605008947876 0.004159772498260459 58-59 0.0 0.0 0.0 1.2787664267379704 0.004217951134599766 60-61 0.0 0.0 0.0 1.4482698837125416 0.004305219089108726 62-63 0.0 0.0 0.0 1.616842482505684 0.004479754998126648 64-65 0.0 0.0 0.0 1.7937928049316865 0.004537933634465955 66-67 0.0 0.0 0.0 2.004195843252791 0.004567022952635608 68-69 0.0 0.0 0.0 2.23263425883908 0.004654290907144569 70-71 0.0 0.0 0.0 2.4770717994186793 0.004799737497992837 72-73 0.0 0.0 0.0 2.735268587492524 0.005352434543216255 74-75 0.0 0.0 0.0 3.0026576001079794 0.005672417043082444 76-77 0.0 0.0 0.0 3.2966924281668377 0.005701506361252097 78-79 0.0 0.0 0.0 3.6315395696177197 0.005846952952100365 80-81 0.0 0.0 0.0 3.9865165192420022 0.00596331022477898 82-83 0.0 0.0 0.0 4.355310894996871 0.006312382042814822 84-85 0.0 0.0 0.0 4.745049579833888 0.006399649997323783 86-87 0.0 0.0 0.0 5.150961925573235 0.006516007270002397 88-89 0.0 0.0 0.0 5.588028931072278 0.006632364542681011 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1965 0.0 44.46544 1 GTATCAA 3105 0.0 39.915924 1 GTGGTAT 875 0.0 32.019257 1 CAACGCA 3925 0.0 31.55843 5 TCAACGC 4025 0.0 30.782427 4 TATCAAC 4110 0.0 30.49409 2 ATCAACG 4035 0.0 30.47262 3 AACGCAG 4315 0.0 28.813574 6 GTACTAG 425 0.0 27.933052 1 TGGTATC 1005 0.0 26.453035 2 ACGCAGA 5050 0.0 24.15007 7 TATACCG 205 2.6961061E-8 23.150517 5 GTACATG 5990 0.0 22.593594 1 CGCAGAG 5450 0.0 22.290514 8 TACATGG 6220 0.0 21.218172 2 TAGTACT 720 0.0 21.098217 4 GTATTAT 325 2.1827873E-11 20.455587 1 CTAGTAC 720 0.0 20.440088 3 ATTATAC 330 2.7284841E-11 20.140379 3 ACATGGG 6430 0.0 19.78688 3 >>END_MODULE