FastQCFastQC Report
Sat 18 Jun 2016
SRR1930915_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930915_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623392
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46520.17732767348531978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23150.057.5390851
GTATCAA43550.042.8207051
TCAACGC50950.033.517584
ATCAACG52450.033.143663
TATCAAC55050.032.952312
CAACGCA52750.031.9425475
AACGCAG56650.028.137556
GTACATG56400.027.8063241
TACATGG57650.025.9748442
ACGCAGA65800.024.3791487
ATAACGC2301.897206E-924.0289483
CGCAGAG67350.023.5644388
ACATGGG60550.023.3164313
TATCACG2704.965841E-1022.3333262
TAACGCC1854.042746E-621.7198144
CATGGGG42750.021.3832234
GTGGTAT12250.020.5164411
GCAGAGT73500.020.4779669
AGAGTAC67750.019.32086410-11
GAGTACA43100.019.2430881
Produced by FastQC (version 0.11.5)