FastQCFastQC Report
Sat 18 Jun 2016
SRR1930915_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930915_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2623392
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49410.18834394554835876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40300.1536179114672912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27200.1036825605933082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33200.054.0575371
GTATCAA57950.040.473991
CAACGCA73900.032.04075
TATCAAC74100.031.9682332
TCAACGC73850.031.9460644
ATCAACG74000.031.6900733
AACGCAG77950.030.6194746
ACGCAGA92800.025.4640377
GTACATG80650.024.9611171
CGCAGAG95650.024.6060878
TACATGG82100.023.8226642
ACATGGG82550.022.9447783
GCAGAGT107900.021.152889
CATGGGG54800.020.2696744
CGTATAC2451.7695129E-719.3759023
GAGTACT65900.018.64919912-13
CAGAGTA107000.018.44826110-11
AGAGTAC100950.018.30825810-11
GAGTACA64350.017.781611
GTGGTAT17150.017.7181511
Produced by FastQC (version 0.11.5)