##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930915_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2623392 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.2281134500677 31.0 31.0 33.0 30.0 34.0 2 31.34357770398019 31.0 31.0 34.0 30.0 34.0 3 31.42308736170576 31.0 31.0 34.0 28.0 34.0 4 35.301041171125014 37.0 35.0 37.0 33.0 37.0 5 35.08754696210097 35.0 35.0 37.0 32.0 37.0 6 35.12843448481966 37.0 35.0 37.0 32.0 37.0 7 34.9998322782108 36.0 35.0 37.0 32.0 37.0 8 34.9729300844098 36.0 35.0 37.0 32.0 37.0 9 36.4723361968017 38.0 35.0 39.0 32.0 39.0 10-11 36.39788163568387 38.0 35.0 39.0 32.0 39.0 12-13 36.29559593076445 38.0 35.0 39.0 32.0 39.0 14-15 37.30445812139398 39.0 36.0 41.0 32.0 41.0 16-17 37.27587909088691 39.0 36.0 40.5 32.0 41.0 18-19 37.2689054857223 39.0 36.0 40.0 31.5 41.0 20-21 37.23309535898562 39.0 36.0 40.0 31.0 41.0 22-23 37.108207237042734 39.0 36.0 40.0 31.0 41.0 24-25 36.98924026603726 39.0 36.0 40.0 31.0 41.0 26-27 36.71781171094521 39.0 35.5 40.0 30.0 41.0 28-29 36.52604147607373 38.0 35.0 40.0 30.0 41.0 30-31 36.27737372074017 38.0 35.0 40.0 30.0 41.0 32-33 36.03867683518132 38.0 34.5 40.0 29.5 41.0 34-35 36.221463662312004 38.0 35.0 40.0 30.0 41.0 36-37 36.27624674467255 38.0 35.0 40.0 30.0 41.0 38-39 36.2341865035801 38.0 35.0 40.0 30.0 41.0 40-41 36.132367751369216 38.0 35.0 40.0 30.0 41.0 42-43 35.986501636049816 38.0 35.0 40.0 29.0 41.0 44-45 35.796875 38.0 34.5 40.0 28.5 41.0 46-47 35.57131549535868 38.0 34.0 40.0 28.0 41.0 48-49 35.36517512441907 38.0 34.0 40.0 27.0 41.0 50-51 35.09542721789195 38.0 34.0 40.0 26.5 41.0 52-53 34.8804507675559 38.0 33.0 40.0 26.0 41.0 54-55 34.710841917639456 38.0 33.0 40.0 26.0 41.0 56-57 34.452110473768315 37.5 33.0 40.0 26.0 41.0 58-59 34.21038392279918 37.0 33.0 40.0 25.0 41.0 60-61 33.897837036935385 37.0 32.5 40.0 24.5 41.0 62-63 33.59513827899147 36.0 32.0 39.0 23.5 41.0 64-65 33.291253461167834 36.0 32.0 39.0 23.0 40.5 66-67 32.95806631262121 35.5 31.0 39.0 22.5 40.0 68-69 32.59137159067345 35.0 31.0 38.5 22.0 40.0 70-71 32.19085481696979 35.0 31.0 38.0 21.5 40.0 72-73 31.77092310260914 35.0 30.5 37.5 20.5 39.5 74-75 31.249741746563227 34.0 30.0 37.0 19.0 39.0 76-77 29.89163952623169 33.0 28.5 35.5 18.0 38.0 78-79 30.6578557455386 34.0 29.5 36.0 18.5 38.5 80-81 30.65700741635257 34.0 30.0 36.0 18.5 37.5 82-83 30.379316930142352 34.0 30.0 35.0 18.0 37.0 84-85 30.04961496413803 34.0 29.5 35.0 17.5 37.0 86-87 29.70865238591869 34.0 29.0 35.0 13.5 36.0 88-89 29.34950628804235 34.0 29.0 35.0 8.5 36.0 90-91 29.00357209292397 34.0 29.0 35.0 6.0 35.5 92-93 28.638940158390362 33.5 29.0 35.0 2.0 35.0 94-95 28.21407913876386 33.0 28.5 35.0 2.0 35.0 96-97 27.747869933277222 33.0 27.0 35.0 2.0 35.0 98-99 27.256250686134592 33.0 27.0 35.0 2.0 35.0 100-101 26.03814050664178 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5023794213931865 1101 2 0.5366686613380764 1101 3 0.5047450992021254 1101 4 0.37166358643311526 1101 5 0.3131858894915922 1101 6 0.37238309076925447 1101 7 0.49935973391352917 1101 8 0.48033417543540224 1101 9 0.623343588311144 1101 10-11 0.5916854580939344 1101 12-13 0.5418865087321052 1101 14-15 0.7824125231398256 1101 16-17 0.7836789717473422 1101 18-19 0.6851884584963699 1101 20-21 0.6972964249761517 1101 22-23 0.8548544442546913 1101 24-25 0.7380224911440578 1101 26-27 0.8215008010015836 1101 28-29 0.8340411488055253 1101 30-31 0.8676150209540694 1101 32-33 0.8963022196556665 1101 34-35 0.8996496191118339 1101 36-37 0.9544898642071615 1101 38-39 0.8484837710852062 1101 40-41 0.8397563060338484 1101 42-43 0.9480200196163011 1101 44-45 0.96431084181215 1101 46-47 1.0122451938159358 1101 48-49 0.8573417276832771 1101 50-51 0.7628418329361821 1101 52-53 0.8378882969852341 1101 54-55 0.8496898574382712 1101 56-57 0.9705271219796785 1101 58-59 0.9649614800841775 1101 60-61 0.9185914377607247 1101 62-63 0.8973902119170418 1101 64-65 0.8816239771113814 1101 66-67 0.8396353462866344 1101 68-69 0.7188502575844851 1101 70-71 0.7847338118205016 1101 72-73 0.7255752714633168 1101 74-75 0.5655324813140901 1101 76-77 0.8614589952692704 1101 78-79 0.8985693368699472 1101 80-81 0.8585059992513031 1101 82-83 0.9489763900420414 1101 84-85 0.9474308490422594 1101 86-87 0.8831269111301765 1101 88-89 0.8444446376641928 1101 90-91 0.8531246509243537 1101 92-93 0.8677177713725612 1101 94-95 0.8901722842166464 1101 96-97 1.0402167070947037 1101 98-99 0.9410746511804469 1101 100-101 1.127484171464726 1102 1 0.40867677848032 1102 2 0.4735604333614596 1102 3 0.49660956575693405 1102 4 0.23505235004577685 1102 5 0.3504052202671559 1102 6 0.37315683477113737 1102 7 0.4698692934992934 1102 8 0.4874522996405517 1102 9 0.5614253657849346 1102 10-11 0.5521718801691549 1102 12-13 0.6305440522017705 1102 14-15 0.7610341046127118 1102 16-17 0.7589402706540582 1102 18-19 0.7614944186763424 1102 20-21 0.6816892924244016 1102 22-23 0.7371465005541822 1102 24-25 0.7737784946066668 1102 26-27 0.7767297180805954 1102 28-29 0.8928777541640258 1102 30-31 0.8488306219415591 1102 32-33 0.7606017889666745 1102 34-35 0.7417059186063355 1102 36-37 0.7834561037471914 1102 38-39 0.8287872304903701 1102 40-41 0.8491788301064744 1102 42-43 0.8421926265137358 1102 44-45 0.9473555351466203 1102 46-47 0.7812235631825075 1102 48-49 0.7810031719341666 1102 50-51 0.7674717396380402 1102 52-53 0.7015196571288769 1102 54-55 0.6942659012980101 1102 56-57 0.659139512570782 1102 58-59 0.8796805169652657 1102 60-61 0.7753600947164117 1102 62-63 0.5783210024397434 1102 64-65 0.7045643111892517 1102 66-67 0.8151218752325704 1102 68-69 0.8389790902356253 1102 70-71 0.8396071165837284 1102 72-73 0.7549735358828507 1102 74-75 0.9295763624311064 1102 76-77 1.0607504424056664 1102 78-79 0.9542735614694529 1102 80-81 0.7754784052317909 1102 82-83 1.112840995528785 1102 84-85 1.0900052043446493 1102 86-87 1.0892802599241413 1102 88-89 1.1165732886351165 1102 90-91 1.105460310476989 1102 92-93 1.1243603208804203 1102 94-95 1.144346120127345 1102 96-97 1.124484268625519 1102 98-99 1.2641269323359374 1102 100-101 1.4429876394195382 1103 1 0.44765813178177183 1103 2 0.5133362115307669 1103 3 0.5132243195114405 1103 4 0.3991254685959902 1103 5 0.4760365651786387 1103 6 0.3999622504939282 1103 7 0.5235246966371463 1103 8 0.4197284973081423 1103 9 0.5779903041186856 1103 10-11 0.6331772602345964 1103 12-13 0.5829212965175685 1103 14-15 0.7783717063410478 1103 16-17 0.7578721231584566 1103 18-19 0.6285286707109066 1103 20-21 0.7153592731274827 1103 22-23 0.6632673475212059 1103 24-25 0.776120343755089 1103 26-27 0.7860758775738432 1103 28-29 0.828789690987584 1103 30-31 0.824583359167832 1103 32-33 0.9409952259160832 1103 34-35 0.9068416787372158 1103 36-37 0.8301682859772441 1103 38-39 0.8297893070068554 1103 40-41 0.9211374675760666 1103 42-43 0.8679065601491018 1103 44-45 0.9398050904784512 1103 46-47 0.9299013570410537 1103 48-49 0.9265444295900025 1103 50-51 0.9786136139563553 1103 52-53 0.8568446634213487 1103 54-55 1.02718602882549 1103 56-57 1.0397392902366889 1103 58-59 0.9588178187523297 1103 60-61 0.9475245485296497 1103 62-63 0.9263511958284525 1103 64-65 0.9671366746425534 1103 66-67 0.8731896137173436 1103 68-69 0.9224222796810579 1103 70-71 0.8151912679387081 1103 72-73 0.8138773868770564 1103 74-75 0.9174080282587767 1103 76-77 0.9770770532790642 1103 78-79 1.1733869518545532 1103 80-81 1.200111082050313 1103 82-83 1.1013394014353217 1103 84-85 1.1959938044788885 1103 86-87 1.1785080684651277 1103 88-89 1.1611153538251493 1103 90-91 1.1200420706917704 1103 92-93 1.0138398283894396 1103 94-95 1.1440645319068565 1103 96-97 1.0787602670314271 1103 98-99 1.2653602572793048 1103 100-101 1.5363802793222163 1104 1 0.4666445602914706 1104 2 0.5296014744417405 1104 3 0.47445629415247836 1104 4 0.29055894761373224 1104 5 0.43455354728908446 1104 6 0.4348304400565084 1104 7 0.45388747241896255 1104 8 0.474369409934539 1104 9 0.48872079413683167 1104 10-11 0.5214294871689305 1104 12-13 0.5677870554345645 1104 14-15 0.6934763938603368 1104 16-17 0.7046722361256101 1104 18-19 0.6941071026281378 1104 20-21 0.702171284683466 1104 22-23 0.6377623519658187 1104 24-25 0.5868749087389702 1104 26-27 0.8000839195383591 1104 28-29 0.6637023652224272 1104 30-31 0.7167412091499301 1104 32-33 0.6863814362078244 1104 34-35 0.6664456224017954 1104 36-37 0.7873048157252569 1104 38-39 0.7843690290953234 1104 40-41 0.7706272634944256 1104 42-43 0.7534491959874714 1104 44-45 0.7332237510461184 1104 46-47 0.6999262113566473 1104 48-49 0.6381452450523071 1104 50-51 0.5292374024917379 1104 52-53 0.5707626943360751 1104 54-55 0.6059528350034924 1104 56-57 0.7211838962097588 1104 58-59 0.6312987439717759 1104 60-61 0.6856122631980952 1104 62-63 0.8162620490081949 1104 64-65 0.7349290002842466 1104 66-67 0.6138842922239505 1104 68-69 0.5851603672028176 1104 70-71 0.6525070018547794 1104 72-73 0.4727613578883272 1104 74-75 0.8019402100062436 1104 76-77 0.8454176631164678 1104 78-79 0.9446400538212885 1104 80-81 0.9306626379721372 1104 82-83 0.9843697914497653 1104 84-85 1.037718073587623 1104 86-87 1.0788380961204744 1104 88-89 1.0329776990354667 1104 90-91 1.0889544995471638 1104 92-93 1.1199660583732651 1104 94-95 1.2142689067602426 1104 96-97 1.0990906882198637 1104 98-99 1.072584598921587 1104 100-101 1.4478274239695494 1105 1 0.12358592586345551 1105 2 0.19834171409863544 1105 3 0.24261567436592912 1105 4 0.17717327979676867 1105 5 0.2343882404048543 1105 6 0.19909253907304247 1105 7 0.20615339558554524 1105 8 0.3252066093159698 1105 9 0.28035946528278544 1105 10-11 0.17149423871344283 1105 12-13 0.2703043669504339 1105 14-15 0.36367297193165626 1105 16-17 0.23984051396305972 1105 18-19 0.29489116765392964 1105 20-21 0.2592116820294095 1105 22-23 0.25371351406559484 1105 24-25 0.4286912657409019 1105 26-27 0.30770631900205814 1105 28-29 0.4279543706158506 1105 30-31 0.35514630243024214 1105 32-33 0.5408018717952672 1105 34-35 0.5475492172551881 1105 36-37 0.48907938893639624 1105 38-39 0.5078264187701222 1105 40-41 0.4246835668668396 1105 42-43 0.4431839812789704 1105 44-45 0.4614622805004629 1105 46-47 0.3177445032870807 1105 48-49 0.22016297935767426 1105 50-51 0.3804111194589268 1105 52-53 0.40148730220833784 1105 54-55 0.4567898439392053 1105 56-57 0.45519997744627716 1105 58-59 0.36057558348698393 1105 60-61 0.4194805585565504 1105 62-63 0.3930476680710555 1105 64-65 0.4227309519719782 1105 66-67 0.35210484360239747 1105 68-69 0.36767402216087675 1105 70-71 0.4206846648572693 1105 72-73 0.3452787074933461 1105 74-75 0.44488800502571024 1105 76-77 0.5171838948051111 1105 78-79 0.33276271610008834 1105 80-81 0.33777845182287436 1105 82-83 0.6090399394641786 1105 84-85 0.5510447032316392 1105 86-87 0.45669497235360623 1105 88-89 0.4435430561973668 1105 90-91 0.38659868600113967 1105 92-93 0.31991863738919335 1105 94-95 0.2636827148366656 1105 96-97 0.2474239072857678 1105 98-99 0.5477398121827015 1105 100-101 0.7797091800314355 1106 1 0.10960458334852063 1106 2 0.10121537879361142 1106 3 0.11372600072477823 1106 4 0.047218304957894475 1106 5 0.1481165003148064 1106 6 0.13773619287958638 1106 7 0.06987282703252617 1106 8 0.11175644598940693 1106 9 0.11258335510356687 1106 10-11 0.05041849050120106 1106 12-13 0.20005849425686506 1106 14-15 0.26856835464553086 1106 16-17 0.1816109150695624 1106 18-19 0.17224468873924081 1106 20-21 0.14844757855983204 1106 22-23 0.17179935615381936 1106 24-25 0.07841979050473213 1106 26-27 0.2755273072013509 1106 28-29 0.23108332503155538 1106 30-31 0.28241963164362716 1106 32-33 0.2564738649679299 1106 34-35 0.1936279082884127 1106 36-37 0.19406452769040783 1106 38-39 0.212809350408385 1106 40-41 0.14710565568118028 1106 42-43 0.21363511573645866 1106 44-45 0.1674053074861277 1106 46-47 0.03904832012590731 1106 48-49 0.16479233105420832 1106 50-51 0.16471558371836892 1106 52-53 -0.03693931855031707 1106 54-55 0.05223729987046255 1106 56-57 0.2654645370531199 1106 58-59 0.23937476538274183 1106 60-61 0.1715037479859376 1106 62-63 0.20323645003744417 1106 64-65 0.20549337803360146 1106 66-67 0.17181438717086195 1106 68-69 0.2952887186088944 1106 70-71 0.2949541095469428 1106 72-73 0.2036267549316193 1106 74-75 0.37262262142899516 1106 76-77 0.3722374541470934 1106 78-79 0.43542670479965295 1106 80-81 0.39225006831444276 1106 82-83 0.3463762450467094 1106 84-85 0.2356655207473075 1106 86-87 0.31905482050404643 1106 88-89 0.41365282342022525 1106 90-91 0.330128158353336 1106 92-93 0.41958904143851683 1106 94-95 0.28686184435022 1106 96-97 0.23518986473257542 1106 98-99 0.2876393148459577 1106 100-101 0.6186978795988445 1107 1 0.12258856210013391 1107 2 0.16315007039635177 1107 3 0.2609275618267546 1107 4 0.15273456918784944 1107 5 0.1758909202054113 1107 6 0.2871798112028685 1107 7 0.15375402190957033 1107 8 0.15666482907206358 1107 9 0.29038712211895046 1107 10-11 0.2583771564474233 1107 12-13 0.0526944801916116 1107 14-15 0.08376855106978809 1107 16-17 0.35891445803699895 1107 18-19 0.23975430241068096 1107 20-21 0.33937107601859395 1107 22-23 0.368376180324006 1107 24-25 0.2542193843541227 1107 26-27 0.39800949866694424 1107 28-29 0.40712990564244933 1107 30-31 0.3973885100979828 1107 32-33 0.4164696437396316 1107 34-35 0.3197272117736105 1107 36-37 0.36046551670550997 1107 38-39 0.4245272519603205 1107 40-41 0.35033777177636694 1107 42-43 0.4404013077516069 1107 44-45 0.35984294909389547 1107 46-47 0.3917461437780858 1107 48-49 0.4144181491447583 1107 50-51 0.3407093079486003 1107 52-53 0.3417028562536828 1107 54-55 0.32607140098672716 1107 56-57 0.23980406896526318 1107 58-59 0.26713456025871807 1107 60-61 0.3754577086826316 1107 62-63 0.22896676622419676 1107 64-65 0.2736129337050244 1107 66-67 0.31482275632031076 1107 68-69 0.39634241146756466 1107 70-71 0.3002341646195319 1107 72-73 0.39624899942108627 1107 74-75 0.5859425992717924 1107 76-77 0.5276950484013696 1107 78-79 0.7562676662007846 1107 80-81 0.6593358478483573 1107 82-83 0.5224326565802961 1107 84-85 0.48700556046909327 1107 86-87 0.43775750767145993 1107 88-89 0.41868134037820326 1107 90-91 0.6005696201848387 1107 92-93 0.6226962077199545 1107 94-95 0.6945794584993656 1107 96-97 0.7496540041967172 1107 98-99 0.7144863874399157 1107 100-101 1.0550290010959387 1108 1 0.2938601980398623 1108 2 0.36910272067448346 1108 3 0.37740769222551407 1108 4 0.14842861413242758 1108 5 0.308397686545149 1108 6 0.25748320227713606 1108 7 0.35436212187527616 1108 8 0.34129314325069515 1108 9 0.41108827601068043 1108 10-11 0.4343110716746992 1108 12-13 0.44211477592882886 1108 14-15 0.46351616904010484 1108 16-17 0.5035271663542105 1108 18-19 0.5090858158535383 1108 20-21 0.5859274018040423 1108 22-23 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2316 68-69 -0.9316995481533432 2316 70-71 -0.8429991628243201 2316 72-73 -0.706165813520041 2316 74-75 -0.4761588838823201 2316 76-77 -0.9525204054982943 2316 78-79 -0.902119702712902 2316 80-81 -0.9382908813573216 2316 82-83 -1.2459128749231247 2316 84-85 -1.1788765264656256 2316 86-87 -0.8977058277918744 2316 88-89 -1.196937748925098 2316 90-91 -0.9282772244514774 2316 92-93 -1.2549823015375026 2316 94-95 -0.9657140175835828 2316 96-97 -0.7620548496174031 2316 98-99 -0.652919637860844 2316 100-101 -0.8502804831609119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 23.0 4 54.0 5 95.0 6 198.0 7 525.0 8 1186.0 9 2055.0 10 3468.0 11 5287.0 12 7316.0 13 9453.0 14 11030.0 15 13451.0 16 15809.0 17 18041.0 18 16375.0 19 14531.0 20 14808.0 21 15789.0 22 17672.0 23 20533.0 24 25090.0 25 31851.0 26 40689.0 27 51721.0 28 65289.0 29 80914.0 30 98648.0 31 118156.0 32 141491.0 33 170444.0 34 208651.0 35 258582.0 36 329006.0 37 411925.0 38 343290.0 39 59933.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.1008644544299 21.804923204085817 14.856652456073865 23.237559885410416 2 15.433845445407135 22.095902175831455 39.84113255002537 22.62911982873604 3 18.095967826476446 26.916125835072574 31.23179994472558 23.7561063937254 4 12.899491361457486 18.151215771569852 38.86144890487708 30.08784396209558 5 12.645491673610726 38.60840316145869 35.75544362047553 12.99066154445506 6 29.974792167846882 39.003030810123235 17.898399437979318 13.123777584050567 7 25.894833863944083 33.310576536026645 23.485662836510897 17.308926763518375 8 24.80140215415767 36.815008965492005 20.892417145436138 17.491171734914186 9 25.55448272865158 16.093102400671498 21.166535983389437 37.18587888728748 10-11 24.06731437772167 26.949651443627182 29.40648976592137 19.57654441272978 12-13 24.530340871665384 24.54846626047499 29.515261158073216 21.40593170978641 14-15 21.8265512740757 26.19276875129603 26.816731925690103 25.163948048938167 16-17 21.522574590453885 29.160758285456385 27.575520547443922 21.74114657664581 18-19 21.454094546297313 28.177241525475416 29.46056098364255 20.908102944584723 20-21 22.142287542235394 27.317743593027654 29.36936226076774 21.170606603969215 22-23 21.95619027289118 27.158390479494088 29.27134356691152 21.614075680703213 24-25 21.788248164818064 27.400040596521087 29.226750629674914 21.584960608985934 26-27 21.74030136471428 27.447544783677863 29.162290871955637 21.64986297965222 28-29 21.798565634814945 27.630026867902437 29.030180600257566 21.54122689702505 30-31 22.05561937698719 27.39248884332121 28.98588348716236 21.566008292529233 32-33 21.906791042883945 27.55191466639011 28.82293203504806 21.718362255677885 34-35 22.012172201114286 27.53365769153642 28.994394260674504 21.459775846674795 36-37 22.10981816158053 27.36559002475058 28.943463243694286 21.58112856997461 38-39 21.99821641594903 27.48676844798264 28.894023506791548 21.620991629276777 40-41 22.00402846609902 27.406453855803935 28.978920311619085 21.61059736647796 42-43 21.97374309896697 27.508712780549242 28.639544396486983 21.8779997239968 44-45 21.919255282304587 27.601562766555027 28.680391671369946 21.79879027977044 46-47 22.138595955518095 27.55797450345554 28.42514750232447 21.878282038701897 48-49 22.096235959735818 27.66676622982774 28.28377826375444 21.953219546682 50-51 22.108512926589682 27.71366339689053 28.170596916195244 22.007226760324546 52-53 22.20240731993679 27.690681832440294 28.14872135083901 21.958189496783902 54-55 21.99123946442467 27.65598294101298 28.280997476456392 22.07178011810596 56-57 22.06185877494754 27.791806958473625 28.079748267701166 22.066585998877667 58-59 22.064296690725328 27.73377413022712 28.19672906184571 22.005200117201852 60-61 22.123029777756166 27.8464687839375 28.167139110013988 21.86336232829235 62-63 22.075997258218333 27.767750565783395 28.159387330458134 21.99686484554013 64-65 21.847584706959708 27.7037355006105 28.157242063492067 22.29143772893773 66-67 21.860442310506425 27.70239654018149 28.1319357251865 22.305225424125588 68-69 21.914347446592355 27.721741287125408 28.119526282904307 22.24438498337793 70-71 21.87180995248087 27.533356772720673 28.144758502135392 22.45007477266306 72-73 21.883731216292222 27.642507468471067 28.0263114478792 22.447449867357513 74-75 21.85416253886029 27.821953134359212 27.909881380935847 22.414002945844643 76-77 21.781978725295563 27.681126035753508 27.96555979197472 22.571335446976207 78-79 21.71217607237589 27.78412500181077 27.908324736942824 22.595374188870515 80-81 21.835548106270984 27.7762297259745 27.85416386333756 22.534058304416956 82-83 21.90488497334748 27.61708886815238 27.942354783425426 22.535671375074713 84-85 21.803951106802018 27.69619021789161 27.818970214701082 22.68088846060529 86-87 21.673638556494797 27.874198747270707 27.799124187311698 22.653038508922798 88-89 21.711436398823352 27.62863428079807 27.764889434917535 22.895039885461046 90-91 21.670591576517634 27.569164027557918 27.87219057621362 22.88805381971083 92-93 21.703658065121232 27.474968183610116 27.906329040567137 22.915044710701515 94-95 21.528465778207426 27.420775704892574 27.955780086706163 23.09497843019384 96-97 21.45474256230102 27.253266000658687 27.990955983703543 23.30103545333675 98-99 21.285610385333186 27.334954135714373 27.877953428233372 23.50148205071907 100-101 21.291061343482028 27.259708042107317 27.874103450799577 23.575127163611082 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 334.0 1 240.0 2 212.0 3 306.5 4 274.0 5 265.5 6 419.5 7 787.5 8 1254.5 9 1464.0 10 1340.5 11 1196.5 12 1228.5 13 1385.0 14 1604.0 15 1767.5 16 1952.0 17 2231.0 18 2521.0 19 2826.0 20 3194.5 21 3735.0 22 4498.5 23 5562.5 24 7232.5 25 9826.0 26 13514.0 27 18485.5 28 25162.0 29 34347.5 30 44163.0 31 53545.5 32 62556.5 33 67508.5 34 70540.5 35 73517.0 36 77492.0 37 81820.0 38 83221.5 39 83056.0 40 82569.0 41 82518.5 42 83408.0 43 85401.0 44 87470.0 45 90197.5 46 92293.0 47 93075.0 48 93683.0 49 92787.5 50 90224.5 51 87604.0 52 85952.0 53 84374.5 54 81141.5 55 74984.5 56 67291.5 57 59490.0 58 51952.5 59 45049.0 60 37726.5 61 30689.0 62 24901.0 63 19312.5 64 14289.0 65 10490.5 66 7659.5 67 5811.5 68 4256.0 69 2982.5 70 2179.0 71 1576.5 72 1145.0 73 820.5 74 576.0 75 391.5 76 283.0 77 174.5 78 90.5 79 55.5 80 36.0 81 23.0 82 11.5 83 8.0 84 6.0 85 3.5 86 1.5 87 2.0 88 2.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017763262219294713 2 0.0027826569570998157 3 0.00445987484905039 4 0.03434484819653334 5 0.0017534550688574181 6 3.8118588453422136E-5 7 0.0 8 0.0 9 3.8118588453422136E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.623717690684427E-5 24-25 5.52719532574621E-4 26-27 0.002248996718751906 28-29 5.717788268013321E-5 30-31 0.007147235335016651 32-33 0.024796141788951096 34-35 0.013951403373952502 36-37 7.242531806150206E-4 38-39 8.57668240201998E-4 40-41 0.00232523389565875 42-43 0.00891974969810078 44-45 0.008519504519339848 46-47 0.008119259340578916 48-49 0.010158603822836999 50-51 0.19318500628194338 52-53 0.05136479794098632 54-55 0.018792464107537113 56-57 0.010978153474585575 58-59 0.02159418035886364 60-61 0.05277518571376295 62-63 0.09409573559727255 64-65 0.09880338127127017 66-67 0.10202440199558434 68-69 0.1002328283382735 70-71 0.09329524523975068 72-73 0.07133893829057952 74-75 0.24721810541466926 76-77 0.2990212671228699 78-79 0.007547480513777583 80-81 8.767275344287091E-4 82-83 0.0 84-85 1.9059294226711068E-5 86-87 0.0 88-89 1.9059294226711067E-4 90-91 0.007223472511923495 92-93 0.020202851880313732 94-95 0.0018678108342176846 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2623392.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.564781582168656 #Duplication Level Percentage of deduplicated Percentage of total 1 79.11887883598381 47.91817615727427 2 11.891032502954928 14.40355572655867 3 3.6173335968092983 6.572490576017871 4 1.6624418393872247 4.027417075821839 5 0.9560503738121864 2.8951491035742882 6 0.5809829651640799 2.111226383287392 7 0.420133370876253 1.7811700089750366 8 0.30199686030760303 1.4632299106424542 9 0.2194067499614375 1.1959489700161159 >10 1.156926418997478 12.29442521889296 >50 0.05224983743198788 2.1231052718077312 >100 0.021117361179881042 2.249068375110308 >500 0.0012602496816832218 0.5188692241338081 >1k 1.8903745224946598E-4 0.4461679978870131 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4941 0.18834394554835876 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4030 0.1536179114672912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2720 0.1036825605933082 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.8118588453422136E-5 1.715336480403996E-4 14-15 0.0 0.0 0.0 3.8118588453422136E-5 2.477708249472439E-4 16-17 0.0 0.0 0.0 3.8118588453422136E-5 3.621265903075103E-4 18-19 0.0 0.0 0.0 7.623717690684427E-5 3.8118588453422134E-4 20-21 0.0 1.9059294226711068E-5 0.0 1.5247435381368854E-4 4.955416498944878E-4 22-23 0.0 3.8118588453422136E-5 0.0 2.85889413400666E-4 5.71778826801332E-4 24-25 0.0 3.8118588453422136E-5 0.0 4.002451787609324E-4 5.71778826801332E-4 26-27 0.0 3.8118588453422136E-5 0.0 9.529647113355533E-4 6.098974152547542E-4 28-29 0.0 3.8118588453422136E-5 0.0 0.001982166599577951 6.480160037081763E-4 30-31 0.0 3.8118588453422136E-5 0.0 0.007452184042644028 6.480160037081763E-4 32-33 0.0 3.8118588453422136E-5 0.0 0.02109863870896915 6.861345921615984E-4 34-35 0.0 3.8118588453422136E-5 0.0 0.04011981434722679 7.623717690684427E-4 36-37 0.0 3.8118588453422136E-5 0.0 0.07067186299264464 7.814310632951538E-4 38-39 0.0 3.8118588453422136E-5 0.0 0.11488942559861431 8.386089459752869E-4 40-41 0.0 3.8118588453422136E-5 0.0 0.16259483904807212 8.386089459752869E-4 42-43 0.0 3.8118588453422136E-5 0.0 0.21420740781400568 8.386089459752869E-4 44-45 0.0 3.8118588453422136E-5 0.0 0.27775109476586035 8.767275344287091E-4 46-47 0.0 3.8118588453422136E-5 0.0 0.35374050084775743 9.339054171088423E-4 48-49 0.0 3.8118588453422136E-5 0.0 0.43424695966138493 9.529647113355533E-4 50-51 0.0 3.8118588453422136E-5 0.0 0.5203187323892121 0.0011054390651492418 52-53 0.0 3.8118588453422136E-5 0.0 0.6059330820555983 0.0012197948305095083 54-55 0.0 3.8118588453422136E-5 0.0 0.6998763432990571 0.0012579134189629304 56-57 0.0 3.8118588453422136E-5 0.0 0.8100771825179005 0.0012960320074163526 58-59 0.0 3.8118588453422136E-5 0.0 0.9331811639282273 0.0013532098900964858 60-61 0.0 3.8118588453422136E-5 0.0 1.066577164220978 0.0014103877727766189 62-63 0.0 3.8118588453422136E-5 0.0 1.2048904624242203 0.0014866249496834631 64-65 0.0 3.8118588453422136E-5 0.0 1.3492455568973298 0.0015247435381368854 66-67 0.0 3.8118588453422136E-5 0.0 1.5124121747722032 0.0015438028323635964 68-69 0.0 3.8118588453422136E-5 0.0 1.6970586172405802 0.0015628621265903076 70-71 0.0 3.8118588453422136E-5 0.0 1.9002306936973201 0.0015819214208170186 72-73 0.0 3.8118588453422136E-5 0.0 2.1119222746734 0.001696277186177285 74-75 0.0 3.8118588453422136E-5 0.0 2.334458594064478 0.0018106329515375514 76-77 0.0 3.8118588453422136E-5 0.0 2.583411095253778 0.0018678108342176846 78-79 0.0 3.8118588453422136E-5 0.0 2.8605522926043836 0.0018868701284443957 80-81 0.0 3.8118588453422136E-5 0.0 3.1648148656395994 0.00196310730535124 82-83 0.0 3.8118588453422136E-5 0.0 3.486764463717203 0.002020285188031373 84-85 0.0 3.8118588453422136E-5 0.0 3.823980556470402 0.002058403776484795 86-87 0.0 3.8118588453422136E-5 0.0 4.188870744440785 0.002229937424525195 88-89 0.0 3.8118588453422136E-5 0.0 4.590335718032227 0.0024205303667923055 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3320 0.0 54.057537 1 GTATCAA 5795 0.0 40.47399 1 CAACGCA 7390 0.0 32.0407 5 TATCAAC 7410 0.0 31.968233 2 TCAACGC 7385 0.0 31.946064 4 ATCAACG 7400 0.0 31.690073 3 AACGCAG 7795 0.0 30.619474 6 ACGCAGA 9280 0.0 25.464037 7 GTACATG 8065 0.0 24.961117 1 CGCAGAG 9565 0.0 24.606087 8 TACATGG 8210 0.0 23.822664 2 ACATGGG 8255 0.0 22.944778 3 GCAGAGT 10790 0.0 21.15288 9 CATGGGG 5480 0.0 20.269674 4 CGTATAC 245 1.7695129E-7 19.375902 3 GAGTACT 6590 0.0 18.649199 12-13 CAGAGTA 10700 0.0 18.448261 10-11 AGAGTAC 10095 0.0 18.308258 10-11 GAGTACA 6435 0.0 17.78161 1 GTGGTAT 1715 0.0 17.718151 1 >>END_MODULE