FastQCFastQC Report
Sat 18 Jun 2016
SRR1930914_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1930914_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2832989
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50700.17896292572967984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20700.055.108581
GTATCAA41800.044.602721
ATCAACG46150.038.202863
TCAACGC45450.038.1112064
CAACGCA47300.036.12625
TATCAAC50800.035.4038852
AACGCAG50200.032.2101946
GTACATG50000.028.3428041
ACGCAGA57250.027.8657637
TACATGG51400.026.6827832
CGCAGAG59050.026.2404888
ACATGGG54850.023.0772673
GCAGAGT65450.022.3506189
CATGGGG41500.020.2039284
GAGTACT43350.019.26045412-13
GTATATA13650.019.1440751
AGAGTAC61450.019.07549310-11
CAGAGTA64250.018.9684410-11
GAGTACA38750.018.4153651
GTACTTT47200.018.04546414-15
Produced by FastQC (version 0.11.5)