##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1930914_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2832989 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 22.95085896909589 30.0 16.0 31.0 2.0 31.0 2 22.479821135909813 30.0 10.0 31.0 2.0 33.0 3 23.125547257684374 30.0 16.0 31.0 2.0 33.0 4 24.873198589899218 33.0 10.0 35.0 2.0 37.0 5 24.76653492124396 33.0 10.0 35.0 2.0 37.0 6 25.187443015133486 32.0 17.0 35.0 2.0 37.0 7 24.713541422151657 32.0 15.0 35.0 2.0 37.0 8 24.61361974931777 32.0 15.0 35.0 2.0 37.0 9 23.14660981740487 27.0 10.0 34.0 2.0 37.0 10-11 23.077187380536955 27.5 10.0 34.5 2.0 37.0 12-13 22.731636974234632 27.0 10.0 34.0 2.0 37.0 14-15 24.77674780946908 29.5 14.0 35.0 2.0 37.5 16-17 26.67577495006158 32.5 17.0 37.0 2.0 38.5 18-19 27.5739849325218 34.0 18.0 38.0 2.0 39.5 20-21 27.744635259790982 34.5 18.0 38.5 2.0 40.0 22-23 28.133163948042156 35.0 18.5 39.0 2.0 40.0 24-25 28.086666061887286 35.0 18.0 39.0 2.0 40.0 26-27 27.923868924305744 35.0 17.5 39.0 2.0 40.0 28-29 28.077387346015108 35.0 18.0 39.0 2.0 40.0 30-31 28.175057509930326 35.0 18.5 39.0 2.0 40.0 32-33 28.06610950483747 35.0 19.0 39.0 2.0 40.0 34-35 24.70391995874322 32.0 8.5 38.0 2.0 40.0 36-37 22.979571399677162 26.0 8.5 37.5 2.0 40.0 38-39 21.955463822838706 26.5 2.0 37.0 2.0 40.0 40-41 23.822148974104735 30.5 5.5 37.5 2.0 40.0 42-43 23.846640597616158 30.5 5.5 38.0 2.0 40.0 44-45 21.52666882928243 23.5 2.0 37.0 2.0 39.5 46-47 22.881030600542395 27.0 7.5 36.5 2.0 39.0 48-49 22.318772681432932 29.5 2.0 37.0 2.0 39.0 50-51 19.68786941989538 16.0 4.5 35.5 2.0 38.5 52-53 23.448925675320304 28.5 7.0 36.5 2.0 39.0 54-55 25.6321139969128 33.0 7.0 38.0 2.0 39.5 56-57 25.98654407059117 34.0 4.5 38.0 2.0 40.0 58-59 25.614096807294345 33.0 2.0 38.0 2.0 40.0 60-61 25.387959501431173 33.0 2.0 38.0 2.0 40.0 62-63 24.974517020715577 32.5 2.0 37.5 2.0 40.0 64-65 24.508772713201502 31.5 2.0 37.0 2.0 39.5 66-67 24.13311364781155 31.0 2.0 36.0 2.0 39.0 68-69 23.71731605735144 31.0 2.0 36.0 2.0 39.0 70-71 23.129067920842616 30.5 2.0 35.5 2.0 38.5 72-73 22.736485916464908 30.0 2.0 35.0 2.0 38.0 74-75 22.214481771725907 29.0 2.0 35.0 2.0 37.0 76-77 21.689102922743437 29.0 2.0 34.0 2.0 37.0 78-79 21.239416919726832 29.0 2.0 34.0 2.0 36.0 80-81 20.7831380566603 28.0 2.0 34.0 2.0 36.0 82-83 20.35199854288174 27.0 2.0 34.0 2.0 35.0 84-85 19.86383409889696 26.0 2.0 34.0 2.0 35.0 86-87 19.319357223060166 26.0 2.0 33.5 2.0 35.0 88-89 18.786304323807823 25.0 2.0 33.0 2.0 35.0 90-91 18.21862809915605 24.5 2.0 33.0 2.0 35.0 92-93 17.58816377331504 21.5 2.0 32.0 2.0 34.5 94-95 16.927778222929916 18.5 2.0 32.0 2.0 34.0 96-97 16.148622003121083 6.5 2.0 32.0 2.0 34.0 98-99 15.232831472342461 2.0 2.0 31.5 2.0 34.0 100-101 13.74180415102212 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 7.917972438032606 1101 2 8.513466316831082 1101 3 7.975925222194665 1101 4 10.273350387065424 1101 5 10.200650738242924 1101 6 9.665502559298371 1101 7 9.917813148720622 1101 8 9.633417424354604 1101 9 11.132673127212318 1101 10-11 10.607701002126138 1101 12-13 9.884537499009038 1101 14-15 8.883884015340147 1101 16-17 7.989825006785139 1101 18-19 7.956370481301857 1101 20-21 8.440881808103512 1101 22-23 8.250233161796213 1101 24-25 8.524134250757513 1101 26-27 8.619849364871133 1101 28-29 8.366919643413699 1101 30-31 8.175293625858469 1101 32-33 8.231469434132435 1101 34-35 11.185023229241686 1101 36-37 4.604850526198913 1101 38-39 8.449412106614211 1101 40-41 10.26675315954607 1101 42-43 10.736733081403067 1101 44-45 4.010186837457827 1101 46-47 5.72810565247503 1101 48-49 9.923290527305163 1101 50-51 -6.044019303254757 1101 52-53 5.420458409274072 1101 54-55 8.187299166940836 1101 56-57 8.703699732237752 1101 58-59 8.991194923559643 1101 60-61 8.874911129535558 1101 62-63 9.05990883547516 1101 64-65 8.961273570513253 1101 66-67 8.996073626299324 1101 68-69 9.026669522677139 1101 70-71 9.185946237419952 1101 72-73 8.956530355356204 1101 74-75 9.076764291184542 1101 76-77 9.071034403699056 1101 78-79 9.031423917828683 1101 80-81 8.817704715961096 1101 82-83 8.601444199842575 1101 84-85 8.684417212070315 1101 86-87 8.500557475211178 1101 88-89 8.643714158638925 1101 90-91 8.709565477793682 1101 92-93 8.742759843124468 1101 94-95 8.633479347828018 1101 96-97 8.646697474191527 1101 98-99 8.726020844092705 1101 100-101 8.443095009746658 1102 1 7.8077218560008 1102 2 8.250913947522243 1102 3 7.999988899840531 1102 4 10.180880972820066 1102 5 10.019026204218516 1102 6 9.475592232633158 1102 7 9.661124357390488 1102 8 9.323446122713264 1102 9 9.964359309395387 1102 10-11 9.332349015763278 1102 12-13 8.366806085736865 1102 14-15 7.661616255406976 1102 16-17 7.264337830460562 1102 18-19 7.49253642215125 1102 20-21 8.102045715851304 1102 22-23 7.946989336890535 1102 24-25 8.289563458837335 1102 26-27 8.526755054257482 1102 28-29 8.275324914658828 1102 30-31 8.07629292918232 1102 32-33 8.167404821259836 1102 34-35 10.972078821468372 1102 36-37 0.12401922935568876 1102 38-39 2.721243777548132 1102 40-41 8.786848109909023 1102 42-43 10.025409281509056 1102 44-45 0.3675757725056492 1102 46-47 0.5383629437421362 1102 48-49 8.208906853020693 1102 50-51 -7.181322730567082 1102 52-53 4.436346251190937 1102 54-55 7.490901156579245 1102 56-57 8.079690304519708 1102 58-59 8.375441639083999 1102 60-61 8.192797973425439 1102 62-63 8.294571060492519 1102 64-65 8.253533281623074 1102 66-67 8.240380094679853 1102 68-69 8.16399328100551 1102 70-71 8.087702136916054 1102 72-73 8.135297703925144 1102 74-75 8.159368701117462 1102 76-77 8.042683874844148 1102 78-79 8.23995502676814 1102 80-81 8.08401566891196 1102 82-83 7.895011364709493 1102 84-85 7.848272889867989 1102 86-87 7.817028489733399 1102 88-89 7.911130667062519 1102 90-91 8.05278849503393 1102 92-93 8.26509414195516 1102 94-95 8.001537378873028 1102 96-97 8.147777051143748 1102 98-99 7.945723281600813 1102 100-101 7.485057549171886 1103 1 7.432846363975187 1103 2 8.07511848815292 1103 3 7.584736590373712 1103 4 9.63324749517528 1103 5 9.411339028957098 1103 6 8.5521513782472 1103 7 8.85632340382153 1103 8 8.191040201347533 1103 9 8.559166874641598 1103 10-11 7.765352833018444 1103 12-13 7.238275214311528 1103 14-15 6.933677533995368 1103 16-17 6.964969305561262 1103 18-19 7.340470029397455 1103 20-21 8.068406133689592 1103 22-23 8.043195289236959 1103 24-25 8.3164804784236 1103 26-27 8.466790792932361 1103 28-29 8.255077143089753 1103 30-31 7.850866712330731 1103 32-33 7.721519545900776 1103 34-35 10.799280961935878 1103 36-37 -4.238569217088326 1103 38-39 -2.437687984633545 1103 40-41 7.320356813790667 1103 42-43 9.888287261846067 1103 44-45 0.2525104112093537 1103 46-47 -0.5415211934183048 1103 48-49 8.221214787604627 1103 50-51 -7.459566649823305 1103 52-53 3.962866435735471 1103 54-55 7.358564510537622 1103 56-57 7.829983002564532 1103 58-59 8.111572881605536 1103 60-61 8.131107037247556 1103 62-63 8.057936409815436 1103 64-65 7.982263489013345 1103 66-67 7.95264024777104 1103 68-69 8.121301312474092 1103 70-71 8.042109005175224 1103 72-73 7.939052527296592 1103 74-75 8.07637131857106 1103 76-77 7.856049375374326 1103 78-79 7.742677653410659 1103 80-81 7.76025886976571 1103 82-83 7.756849207136526 1103 84-85 7.7109627011758946 1103 86-87 7.677932095332007 1103 88-89 7.585978224947734 1103 90-91 7.721226769376088 1103 92-93 7.6748414432696705 1103 94-95 7.6767064481856515 1103 96-97 7.827877997107535 1103 98-99 7.596775633696872 1103 100-101 7.265698943468548 1104 1 7.218261609877928 1104 2 7.8614886630958765 1104 3 7.310565010805572 1104 4 9.419051652042764 1104 5 8.919482716955198 1104 6 8.115257013470458 1104 7 8.19820952098475 1104 8 7.484641078779859 1104 9 7.3849022616854185 1104 10-11 6.539258202921413 1104 12-13 5.908695406147189 1104 14-15 6.233731401787377 1104 16-17 6.566807266719138 1104 18-19 7.365265848395968 1104 20-21 7.878067595588561 1104 22-23 8.009111997508608 1104 24-25 8.095815229858218 1104 26-27 8.088456625303397 1104 28-29 8.129569195394435 1104 30-31 7.993598848092059 1104 32-33 7.791778749239398 1104 34-35 10.675300329745838 1104 36-37 -4.224128454475867 1104 38-39 -2.696843230929364 1104 40-41 6.875205667200554 1104 42-43 9.339740918141665 1104 44-45 -0.09563753482579074 1104 46-47 -1.2764419722987057 1104 48-49 7.668753421495023 1104 50-51 -7.6121176894464355 1104 52-53 3.69826451443625 1104 54-55 6.83611709010324 1104 56-57 7.289977877108903 1104 58-59 7.663557332194134 1104 60-61 7.465418174583743 1104 62-63 7.703980602266856 1104 64-65 7.834182345189088 1104 66-67 7.675190704357124 1104 68-69 7.714144637023807 1104 70-71 7.794352572360584 1104 72-73 7.663257996579379 1104 74-75 7.475458201302544 1104 76-77 7.533981686639688 1104 78-79 7.558838496093539 1104 80-81 7.441491159740888 1104 82-83 7.238627817099342 1104 84-85 7.069111544275575 1104 86-87 7.07194359115363 1104 88-89 7.054740944570707 1104 90-91 7.243586020554488 1104 92-93 7.165173074961981 1104 94-95 7.169213928023932 1104 96-97 7.003258450741107 1104 98-99 6.752702084506609 1104 100-101 6.480500222812141 1105 1 6.375399414861903 1105 2 7.14878505696695 1105 3 6.810888281733995 1105 4 8.915073665273983 1105 5 8.325979542652906 1105 6 7.419636917357796 1105 7 7.182057527594797 1105 8 6.472783603818915 1105 9 5.5923359740294885 1105 10-11 5.333346602437214 1105 12-13 4.6357955332733205 1105 14-15 5.18630401579761 1105 16-17 6.106284669631954 1105 18-19 6.890655346005989 1105 20-21 7.478439849229794 1105 22-23 7.503512628389682 1105 24-25 7.552414289184739 1105 26-27 7.671411614693856 1105 28-29 7.3810668105492745 1105 30-31 7.2151104103228825 1105 32-33 7.1707721400497775 1105 34-35 9.96736614650878 1105 36-37 -4.708677344978376 1105 38-39 -3.095299574805768 1105 40-41 6.095397456076181 1105 42-43 8.332861195037871 1105 44-45 -4.3407618347441925 1105 46-47 -5.44153740889417 1105 48-49 5.85441886967347 1105 50-51 -12.24860712548308 1105 52-53 0.46401498650594064 1105 54-55 5.305863178011535 1105 56-57 6.354969775010918 1105 58-59 6.936719180747591 1105 60-61 6.776495680874799 1105 62-63 6.699496314603223 1105 64-65 6.627497066018645 1105 66-67 6.457474847736233 1105 68-69 6.286856741049011 1105 70-71 6.510370400827167 1105 72-73 6.350140684961179 1105 74-75 6.3458235102883656 1105 76-77 6.209340975212587 1105 78-79 6.060713242836119 1105 80-81 6.066995642103045 1105 82-83 5.97408538559705 1105 84-85 5.923235002962148 1105 86-87 5.8965609290870695 1105 88-89 5.885571245946505 1105 90-91 5.72234882142002 1105 92-93 5.6780682902527175 1105 94-95 5.783888780797664 1105 96-97 5.421743792725806 1105 98-99 5.254578221891833 1105 100-101 5.24113762279819 1106 1 6.359206939721151 1106 2 7.272418983074797 1106 3 6.930452789403976 1106 4 8.61631583784909 1106 5 7.972384456158938 1106 6 7.191619843935335 1106 7 6.927779377826379 1106 8 6.176399226442779 1106 9 5.460808380842849 1106 10-11 4.570931932689778 1106 12-13 4.2268533124646375 1106 14-15 4.872382889280306 1106 16-17 6.118820502545788 1106 18-19 6.934401304600943 1106 20-21 7.740291144480366 1106 22-23 7.51830369696351 1106 24-25 7.843220474456444 1106 26-27 7.807177812434677 1106 28-29 7.682073923422163 1106 30-31 7.339574364675816 1106 32-33 7.315689553213538 1106 34-35 10.205322338204596 1106 36-37 -0.4825884561830982 1106 38-39 1.0162227737692362 1106 40-41 7.730953395440885 1106 42-43 9.03047658175953 1106 44-45 -4.149464520027475 1106 46-47 -5.178205406021217 1106 48-49 6.154811373038239 1106 50-51 -12.075583806970865 1106 52-53 0.8811013098646825 1106 54-55 5.733088978552004 1106 56-57 6.561190537354186 1106 58-59 7.087880841162143 1106 60-61 7.209344905662224 1106 62-63 7.255878838461996 1106 64-65 7.071926772425709 1106 66-67 7.05154722548307 1106 68-69 6.759230801915994 1106 70-71 6.784954149271158 1106 72-73 6.745104172775353 1106 74-75 6.663318449975332 1106 76-77 6.582771751604671 1106 78-79 6.607466586012752 1106 80-81 6.3571045467795955 1106 82-83 6.432749262860501 1106 84-85 6.556774241714141 1106 86-87 6.5866593024149545 1106 88-89 6.471371354630019 1106 90-91 6.314484168206803 1106 92-93 6.167088622074452 1106 94-95 6.005039414890167 1106 96-97 5.881177142979503 1106 98-99 5.4374053308470565 1106 100-101 5.239243296456971 1107 1 5.882244264927092 1107 2 6.908889359444135 1107 3 6.579793105723564 1107 4 7.918333117685979 1107 5 7.864115135873245 1107 6 6.190823358035761 1107 7 6.412500813122826 1107 8 5.551988297430388 1107 9 4.494878346910845 1107 10-11 3.9383009701034446 1107 12-13 3.6437811916827094 1107 14-15 4.5833053195785745 1107 16-17 6.289791840427256 1107 18-19 7.002943481599207 1107 20-21 7.782872704510112 1107 22-23 7.687182771805592 1107 24-25 7.8433204169552795 1107 26-27 7.766424652503506 1107 28-29 7.634652208202471 1107 30-31 7.469347698458481 1107 32-33 7.2496508884200495 1107 34-35 10.055860279494432 1107 36-37 -0.5932927295937596 1107 38-39 0.7459650927300849 1107 40-41 7.595234483455577 1107 42-43 9.029895093342958 1107 44-45 -4.094999122879784 1107 46-47 -5.076641711371352 1107 48-49 6.11761965194205 1107 50-51 -12.077322901232755 1107 52-53 0.9618963424381413 1107 54-55 5.7781578325095815 1107 56-57 6.858881117905312 1107 58-59 7.246530132507697 1107 60-61 7.321313151539723 1107 62-63 7.102038687484583 1107 64-65 7.199856909564929 1107 66-67 6.9729572466932055 1107 68-69 6.8838254503058565 1107 70-71 6.928721117906694 1107 72-73 7.070968585873825 1107 74-75 7.004069924890711 1107 76-77 6.989653882644138 1107 78-79 6.887310374517998 1107 80-81 6.799068653837072 1107 82-83 6.705343534228081 1107 84-85 6.546931221997351 1107 86-87 6.516671750469076 1107 88-89 6.6890262655032195 1107 90-91 6.577466161706855 1107 92-93 6.393355122064975 1107 94-95 6.220166486143167 1107 96-97 6.148275735359441 1107 98-99 5.907772485255585 1107 100-101 5.504664350307971 1108 1 5.601464630954112 1108 2 6.023596239909359 1108 3 6.309728226372375 1108 4 7.470651309159887 1108 5 7.084473856658672 1108 6 6.667574596714914 1108 7 6.21028794045586 1108 8 5.406831435874128 1108 9 2.48255262238332 1108 10-11 3.148758654363373 1108 12-13 3.0360336619528816 1108 14-15 4.28964943095087 1108 16-17 5.607491888364876 1108 18-19 6.743113053820341 1108 20-21 7.8900856251583065 1108 22-23 7.970261197567506 1108 24-25 7.541639394449028 1108 26-27 7.799678046194327 1108 28-29 7.6312899232837985 1108 30-31 7.272412379778036 1108 32-33 7.42760853887502 1108 34-35 10.258297355378502 1108 36-37 -4.643052321805623 1108 38-39 -2.758196515914907 1108 40-41 6.473083478226933 1108 42-43 8.697420751307426 1108 44-45 -4.149128933052939 1108 46-47 -5.252963147249016 1108 48-49 6.4033827367514995 1108 50-51 -12.244779095457297 1108 52-53 0.6664462283082351 1108 54-55 5.935680602876197 1108 56-57 6.835168683394244 1108 58-59 7.356638120815976 1108 60-61 7.173261603992287 1108 62-63 7.268541963512327 1108 64-65 7.134153753315786 1108 66-67 7.020388658610784 1108 68-69 6.959206966538996 1108 70-71 6.924176586306036 1108 72-73 6.748186016757948 1108 74-75 6.726756062632617 1108 76-77 6.721723513882186 1108 78-79 6.512500336582288 1108 80-81 6.410309053773741 1108 82-83 6.528010130522272 1108 84-85 6.430434096291318 1108 86-87 6.52938743156486 1108 88-89 6.348607866968479 1108 90-91 6.247657013604925 1108 92-93 6.43529644494247 1108 94-95 6.366569278563702 1108 96-97 6.403823129258761 1108 98-99 6.213202532325177 1108 100-101 5.826421593184174 1109 1 4.560313744369971 1109 2 6.39261597895252 1109 3 6.097617152434605 1109 4 7.0644954041749415 1109 5 6.500332003195975 1109 6 4.409628795443588 1109 7 3.8071430759526805 1109 8 2.280702295693466 1109 9 1.0037971857824495 1109 10-11 0.5483906383044932 1109 12-13 1.0760135883496709 1109 14-15 2.8011917527976387 1109 16-17 2.8125772011785273 1109 18-19 4.628024395406161 1109 20-21 5.334548656272389 1109 22-23 6.796791809812401 1109 24-25 6.736353345603263 1109 26-27 5.391514780888205 1109 28-29 5.38003532308306 1109 30-31 5.328451188744243 1109 32-33 5.058086959751563 1109 34-35 7.9854636317251035 1109 36-37 -5.181526727961526 1109 38-39 -3.35572077151879 1109 40-41 4.809985452131251 1109 42-43 5.296115967098661 1109 44-45 -5.226077745364751 1109 46-47 -6.958215740089429 1109 48-49 3.533359317547749 1109 50-51 -12.332433829482053 1109 52-53 -0.6090639757733953 1109 54-55 3.5828870264292227 1109 56-57 5.08023392260468 1109 58-59 5.801101151930062 1109 60-61 5.984569733801589 1109 62-63 5.893500143505637 1109 64-65 5.617425750639306 1109 66-67 5.815303345797137 1109 68-69 5.713472607221497 1109 70-71 5.645822621769401 1109 72-73 5.399239880926569 1109 74-75 5.528361950889558 1109 76-77 5.694791429573055 1109 78-79 5.857933591851609 1109 80-81 5.502313068494381 1109 82-83 5.274079049893299 1109 84-85 5.133679328810555 1109 86-87 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-16.34300385726982 2313 88-89 -15.80949185741335 2313 90-91 -15.242006899505842 2313 92-93 -14.673138835758976 2313 94-95 -14.458288533413548 2313 96-97 -13.57813205915601 2313 98-99 -12.504251192273513 2313 100-101 -10.997521333743649 2314 1 -20.443700976272385 2314 2 -19.97540007993005 2314 3 -20.594587598318157 2314 4 -22.257351367320542 2314 5 -22.1694572239703 2314 6 -22.606764647179364 2314 7 -22.11390075141433 2314 8 -22.05720335837144 2314 9 -20.59573443014261 2314 10-11 -20.543446096389385 2314 12-13 -20.222581259425507 2314 14-15 -22.242802894241503 2314 16-17 -24.141922631541448 2314 18-19 -25.02068754838775 2314 20-21 -25.172309824824964 2314 22-23 -25.52321488138867 2314 24-25 -25.4829507694854 2314 26-27 -25.339833496127522 2314 28-29 -25.48496969531774 2314 30-31 -25.57318815551805 2314 32-33 -25.45813920284462 2314 34-35 -22.274322249840637 2314 36-37 -20.514413981223488 2314 38-39 -19.552274802967435 2314 40-41 -21.384393938969453 2314 42-43 -21.40344910483175 2314 44-45 -19.09760668480938 2314 46-47 -20.34848004253172 2314 48-49 -19.896884911291327 2314 50-51 -17.26686005899025 2314 52-53 -20.92923794700123 2314 54-55 -23.036216352627317 2314 56-57 -23.357872467492342 2314 58-59 -22.976416412472748 2314 60-61 -22.754306144418553 2314 62-63 -22.355914376400687 2314 64-65 -21.909171773616297 2314 66-67 -21.53682111054278 2314 68-69 -21.139153689198707 2314 70-71 -20.551233249759537 2314 72-73 -20.153118767450817 2314 74-75 -19.630219514483475 2314 76-77 -19.132575582805668 2314 78-79 -18.69107945599048 2314 80-81 -18.270520655159014 2314 82-83 -17.83833312809265 2314 84-85 -17.361804110466796 2314 86-87 -16.812532943142397 2314 88-89 -16.279194073479832 2314 90-91 -15.716210500613876 2314 92-93 -15.117564043515209 2314 94-95 -14.458288533413548 2314 96-97 -13.691359205970414 2314 98-99 -12.80653651083307 2314 100-101 -11.34637867446387 2315 1 -20.443700976272385 2315 2 -19.97540007993005 2315 3 -20.594587598318157 2315 4 -21.65097211740817 2315 5 -22.1694572239703 2315 6 -22.606764647179364 2315 7 -22.11390075141433 2315 8 -22.05720335837144 2315 9 -20.06821953354254 2315 10-11 -19.855573681387632 2315 12-13 -19.150026054378188 2315 14-15 -21.070002333428324 2315 16-17 -23.566738614717053 2315 18-19 -25.02068754838775 2315 20-21 -25.172309824824964 2315 22-23 -25.52321488138867 2315 24-25 -25.4829507694854 2315 26-27 -25.339833496127522 2315 28-29 -25.48496969531774 2315 30-31 -25.57318815551805 2315 32-33 -25.45813920284462 2315 34-35 -22.274322249840637 2315 36-37 -20.514413981223488 2315 38-39 -19.552274802967435 2315 40-41 -21.384393938969453 2315 42-43 -21.40344910483175 2315 44-45 -19.09760668480938 2315 46-47 -20.34848004253172 2315 48-49 -19.896884911291327 2315 50-51 -17.26686005899025 2315 52-53 -20.92923794700123 2315 54-55 -23.036216352627317 2315 56-57 -23.357872467492342 2315 58-59 -22.976416412472748 2315 60-61 -22.754306144418553 2315 62-63 -22.355914376400687 2315 64-65 -21.909171773616297 2315 66-67 -21.53682111054278 2315 68-69 -21.139153689198707 2315 70-71 -20.551233249759537 2315 72-73 -20.153118767450817 2315 74-75 -19.630219514483475 2315 76-77 -19.132575582805668 2315 78-79 -18.69107945599048 2315 80-81 -18.270520655159014 2315 82-83 -17.83833312809265 2315 84-85 -17.361804110466796 2315 86-87 -16.812532943142397 2315 88-89 -16.279194073479832 2315 90-91 -15.716210500613876 2315 92-93 -15.117564043515209 2315 94-95 -14.458288533413548 2315 96-97 -13.691359205970414 2315 98-99 -12.80653651083307 2315 100-101 -11.34637867446387 2316 1 -20.443700976272385 2316 2 -19.97540007993005 2316 3 -20.594587598318157 2316 4 -22.257351367320542 2316 5 -22.1694572239703 2316 6 -22.606764647179364 2316 7 -22.11390075141433 2316 8 -22.05720335837144 2316 9 -20.59573443014261 2316 10-11 -20.543446096389385 2316 12-13 -20.222581259425507 2316 14-15 -22.242802894241503 2316 16-17 -24.141922631541448 2316 18-19 -25.02068754838775 2316 20-21 -25.172309824824964 2316 22-23 -25.52321488138867 2316 24-25 -25.4829507694854 2316 26-27 -25.339833496127522 2316 28-29 -25.48496969531774 2316 30-31 -25.57318815551805 2316 32-33 -25.45813920284462 2316 34-35 -22.274322249840637 2316 36-37 -20.514413981223488 2316 38-39 -19.552274802967435 2316 40-41 -21.384393938969453 2316 42-43 -21.40344910483175 2316 44-45 -19.09760668480938 2316 46-47 -20.34848004253172 2316 48-49 -19.896884911291327 2316 50-51 -17.26686005899025 2316 52-53 -20.92923794700123 2316 54-55 -23.036216352627317 2316 56-57 -23.357872467492342 2316 58-59 -22.976416412472748 2316 60-61 -22.754306144418553 2316 62-63 -22.355914376400687 2316 64-65 -21.909171773616297 2316 66-67 -21.53682111054278 2316 68-69 -21.139153689198707 2316 70-71 -20.551233249759537 2316 72-73 -20.153118767450817 2316 74-75 -19.630219514483475 2316 76-77 -19.132575582805668 2316 78-79 -18.69107945599048 2316 80-81 -18.270520655159014 2316 82-83 -17.83833312809265 2316 84-85 -17.361804110466796 2316 86-87 -16.812532943142397 2316 88-89 -16.279194073479832 2316 90-91 -15.716210500613876 2316 92-93 -15.117564043515209 2316 94-95 -14.458288533413548 2316 96-97 -13.691359205970414 2316 98-99 -12.80653651083307 2316 100-101 -11.34637867446387 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 447059.0 3 10532.0 4 12269.0 5 8859.0 6 10858.0 7 16446.0 8 24880.0 9 29852.0 10 30552.0 11 29581.0 12 29139.0 13 29073.0 14 31274.0 15 35330.0 16 39083.0 17 41322.0 18 44253.0 19 47151.0 20 50910.0 21 55527.0 22 61516.0 23 67727.0 24 74309.0 25 82834.0 26 91779.0 27 103118.0 28 115050.0 29 128610.0 30 141097.0 31 146697.0 32 148045.0 33 153627.0 34 153639.0 35 144010.0 36 119938.0 37 65519.0 38 11440.0 39 84.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.56137619562819 20.81633690938367 14.811886190878898 23.810400704109245 2 14.524803637407102 20.755153475356522 41.28795175630796 23.432091130928416 3 17.694703707708737 26.536374204187563 30.718909246968817 25.050012841134883 4 11.773443432469998 17.118437041447514 40.961589909841095 30.146529616241395 5 12.442454898296557 37.910677064615825 36.864128872277725 12.78273916480989 6 28.991620496590997 39.37051433533879 18.475197588439876 13.162667579630332 7 24.947890260863765 34.73508977784221 23.36460260892593 16.952417352368094 8 23.32898160347085 39.04873817783378 20.73681048914481 16.885469729550557 9 24.417969820576587 20.244509015515717 20.682799095358806 34.654722068548885 10-11 22.8435198530823 30.687171420292252 28.141080883764165 18.328227842861285 12-13 23.195290270425804 28.109960676133017 28.332512753995186 20.362236299445996 14-15 21.26959251816202 27.93141901733838 26.263726204778205 24.53526225972139 16-17 21.248530365183186 29.95180192594032 27.3243228775301 21.475344831346394 18-19 21.289779884179364 28.796871247023514 29.183145149926247 20.73020371887087 20-21 22.012070154035115 27.838449118128622 29.09696412029762 21.052516607538642 22-23 21.886113904614366 27.618040385871147 28.875378531992347 21.620467177522144 24-25 21.734810272858834 27.67045703999649 28.89481776137961 21.699914925765068 26-27 21.69437863949513 27.81613666112125 28.72366388522497 21.76582081415865 28-29 21.71403631123095 27.926573138410145 28.747274420996444 21.612116129362462 30-31 22.011045037337283 27.665903276019392 28.6918450035052 21.63120668313812 32-33 21.84548318720744 27.814693875429114 28.523372312013663 21.816450625349784 34-35 22.16308582318937 27.741991983095655 28.51075553644154 21.58416665727344 36-37 22.634745108645156 27.745404853960782 28.161500030816754 21.458350006577305 38-39 22.366635584454407 27.765704573998907 28.248794493193497 21.618865348353193 40-41 22.276905953559062 27.64354229952136 28.385397896685603 21.69415385023397 42-43 22.379015549132404 27.722849680328153 28.070930154073025 21.82720461646641 44-45 22.71930741733895 27.79044543847786 27.802225297567002 21.68802184661619 46-47 22.045882492428948 27.76433375735251 28.17683810304671 22.012945647171833 48-49 22.56581387884613 27.79762274104823 27.685598642586655 21.950964737518987 50-51 22.962717080335405 27.787570731858953 27.413620261090344 21.8360919267153 52-53 21.962150666438575 27.88653417569389 28.037157945764378 22.11415721210316 54-55 21.775665025403093 27.817979989170837 28.15875882433011 22.247596161095963 56-57 21.873778745603484 27.967520683074458 27.9782807218106 22.18041984951146 58-59 22.017710698825084 27.817535792325632 28.07303923908392 22.091714269765365 60-61 21.993294174829263 27.962149681717435 28.015878262368677 22.028677881084626 62-63 21.935832790432958 27.800181064973728 27.972309269257433 22.29167687533588 64-65 21.73202385227831 27.69232926777961 28.00506573319293 22.570581146749152 66-67 21.834105033383157 27.758224319980467 27.998191321775185 22.409479324861188 68-69 21.855494145784935 27.720079044764795 27.999741762891933 22.424685046558334 70-71 21.779933313125188 27.579982011439135 27.998727880778578 22.6413567946571 72-73 21.87130084223105 27.65673671342965 27.909806724465557 22.562155719873743 74-75 21.83433918934617 27.834091841226215 27.763364503411115 22.568204466016503 76-77 21.77079652963945 27.625927700228736 27.88657446721432 22.716701302917492 78-79 21.660066244454068 27.683395744003285 27.873635616589027 22.78290239495362 80-81 21.778920947300186 27.748711155535847 27.813118644350382 22.659249252813584 82-83 21.872331862133105 27.56966709454562 27.932152856336224 22.625848186985056 84-85 21.90482007911917 27.630450908235073 27.72230970524196 22.742419307403797 86-87 21.706130729812294 27.726466860684816 27.75186112173821 22.815541287764677 88-89 21.82987862645021 27.481630284362307 27.675212912121978 23.01327817706551 90-91 21.81459945624537 27.454005746673403 27.792538598386667 22.93885619869456 92-93 21.88812121819773 27.282226017812782 27.7785847903309 23.051067973658583 94-95 21.64508042367696 27.34316078569023 27.831489747226318 23.180269043406494 96-97 21.643154538892855 27.099423554608908 27.78866029056309 23.468761615935147 98-99 21.527755419038545 27.188896575932503 27.73588513515105 23.547462869877897 100-101 21.728227218480807 27.142365979818166 27.65032235768818 23.479084444012848 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 37802.0 1 36194.5 2 24717.0 3 17412.5 4 23273.5 5 27612.5 6 27995.5 7 25770.0 8 22559.5 9 19601.0 10 17047.0 11 14553.0 12 12235.5 13 10365.0 14 8879.5 15 7776.0 16 7115.0 17 6834.0 18 6873.0 19 7216.0 20 7749.5 21 8537.5 22 9781.0 23 11497.5 24 13871.0 25 17163.0 26 21660.5 27 27618.5 28 35001.0 29 44236.5 30 53775.0 31 62223.0 32 69657.0 33 74997.5 34 78584.5 35 80937.0 36 83221.5 37 84906.0 38 84160.5 39 81997.0 40 80445.0 41 80112.0 42 80121.5 43 80390.5 44 81045.5 45 82300.0 46 83052.0 47 82864.5 48 82358.5 49 80844.5 50 78081.0 51 75490.5 52 72859.0 53 69480.5 54 65538.5 55 60043.0 56 53362.0 57 46588.0 58 40414.5 59 34564.0 60 28654.0 61 23222.0 62 18541.0 63 14514.5 64 11136.0 65 8405.0 66 6230.5 67 4669.0 68 3531.5 69 2624.0 70 2015.5 71 1501.0 72 1066.0 73 759.0 74 548.5 75 409.0 76 266.0 77 161.5 78 107.5 79 68.0 80 41.0 81 22.5 82 13.0 83 7.5 84 6.5 85 5.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 13.93337566788999 2 20.657828180765968 3 16.43483260965715 4 21.62687536026437 5 21.42292116206593 6 15.45572538403785 7 14.293737109462832 8 11.505162921564468 9 2.8889275602552638 10-11 6.202600857257123 12-13 3.3922299027634772 14-15 7.065329233540971 16-17 10.981475748758642 18-19 13.95489004722574 20-21 14.357856666580773 22-23 12.320944415950786 24-25 14.776866412118084 26-27 12.423680430810004 28-29 12.412173149983992 30-31 13.194015225615066 32-33 11.731372765654932 34-35 18.579775636262617 36-37 23.256249847775617 38-39 22.416412488717746 40-41 17.47985961117392 42-43 17.372146520865417 44-45 22.69016928763225 46-47 12.174208936215425 48-49 20.92651965821258 50-51 20.511428035901304 52-53 10.654753689477792 54-55 12.219796829426446 56-57 11.75429908128835 58-59 12.736053687465782 60-61 12.162260425296392 62-63 12.11706081456723 64-65 13.16385273645609 66-67 11.513846329795138 68-69 12.518456654791105 70-71 11.207297310367249 72-73 10.585833548947772 74-75 10.665166719673108 76-77 10.733804472943593 78-79 11.104508347896868 80-81 11.079181740557411 82-83 9.666610071553402 84-85 9.562497418803956 86-87 9.718516379696498 88-89 11.044042175949148 90-91 8.650121832453285 92-93 10.576814805846405 94-95 10.70025333667021 96-97 10.260682268798078 98-99 10.10010981334555 100-101 10.21760056251542 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2832989.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 96.84719649838516 #Duplication Level Percentage of deduplicated Percentage of total 1 98.8075125473285 95.69230583187782 2 0.8258474274480694 1.5996201616749812 3 0.16821686458530605 0.4887399521650615 4 0.06394135133553822 0.2477016246866061 5 0.03646645933963401 0.1765837176632954 6 0.020306977499730414 0.11800043041228064 7 0.016249463507189903 0.11016004896919117 8 0.01084995437141731 0.08406301304057322 9 0.007587772066320853 0.06613690070627404 >10 0.03815698821333821 0.6483699476695324 >50 0.0030644592600029595 0.20025338679451601 >100 0.0016539889380246336 0.27545892308825576 >500 3.643652671173911E-5 0.021454954809996384 >1k 7.287305340424961E-5 0.09224219055679633 >5k 3.643652670212481E-5 0.1789089158848345 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5070 0.17896292572967984 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.529840744175145E-5 0.0 2 0.0 0.0 0.0 3.529840744175145E-5 0.0 3 0.0 0.0 0.0 3.529840744175145E-5 0.0 4 0.0 0.0 0.0 3.529840744175145E-5 0.0 5 0.0 0.0 0.0 3.529840744175145E-5 0.0 6 0.0 0.0 0.0 3.529840744175145E-5 7.05968148835029E-5 7 0.0 0.0 0.0 3.529840744175145E-5 7.05968148835029E-5 8 0.0 0.0 0.0 3.529840744175145E-5 7.05968148835029E-5 9 0.0 0.0 0.0 3.529840744175145E-5 7.05968148835029E-5 10-11 1.7649203720875726E-5 0.0 0.0 3.529840744175145E-5 7.05968148835029E-5 12-13 3.529840744175145E-5 0.0 0.0 3.529840744175145E-5 3.000364632548873E-4 14-15 3.529840744175145E-5 0.0 0.0 5.294761116262718E-5 3.882824818592659E-4 16-17 3.529840744175145E-5 0.0 0.0 7.05968148835029E-5 4.5887929674276885E-4 18-19 3.529840744175145E-5 0.0 0.0 8.824601860437863E-5 4.941777041845203E-4 20-21 3.529840744175145E-5 3.529840744175145E-5 0.0 1.0589522232525435E-4 4.941777041845203E-4 22-23 1.0589522232525435E-4 1.0589522232525435E-4 0.0 1.7649203720875725E-4 4.941777041845203E-4 24-25 1.411936297670058E-4 1.0589522232525435E-4 0.0 3.529840744175145E-4 4.941777041845203E-4 26-27 1.411936297670058E-4 1.2354442604613008E-4 0.0 5.11826907905396E-4 5.294761116262718E-4 28-29 1.411936297670058E-4 1.411936297670058E-4 0.0 0.0010060046120899163 5.294761116262718E-4 30-31 1.7649203720875725E-4 1.411936297670058E-4 0.0 0.0034945423367333936 5.294761116262718E-4 32-33 1.7649203720875725E-4 1.411936297670058E-4 0.0 0.010995453918105576 5.294761116262718E-4 34-35 1.7649203720875725E-4 1.411936297670058E-4 0.0 0.02154967774318926 5.294761116262718E-4 36-37 1.7649203720875725E-4 1.7649203720875725E-4 0.0 0.03813992924081244 5.294761116262718E-4 38-39 1.7649203720875725E-4 1.7649203720875725E-4 0.0 0.062107547893761675 5.294761116262718E-4 40-41 1.7649203720875725E-4 1.7649203720875725E-4 0.0 0.08796363134484461 5.294761116262718E-4 42-43 1.7649203720875725E-4 1.7649203720875725E-4 0.0 0.11419034807406594 6.000729265097746E-4 44-45 1.7649203720875725E-4 1.7649203720875725E-4 0.0 0.14887103338558674 6.530205376724019E-4 46-47 1.7649203720875725E-4 1.9414124092963296E-4 0.0 0.1920233364831279 6.706697413932775E-4 48-49 1.7649203720875725E-4 2.117904446505087E-4 0.0 0.23584630932206233 6.883189451141532E-4 50-51 1.7649203720875725E-4 2.2943964837138442E-4 0.0 0.2866760160381844 7.412665562767805E-4 52-53 1.7649203720875725E-4 2.4708885209226016E-4 0.0 0.3549960836416943 7.942141674394077E-4 54-55 1.7649203720875725E-4 2.4708885209226016E-4 0.0 0.42982870741820745 8.118633711602834E-4 56-57 1.7649203720875725E-4 2.823872595340116E-4 0.0 0.5182865164672366 8.648109823229106E-4 58-59 1.7649203720875725E-4 3.1768566697576304E-4 0.0 0.618481045990648 9.177585934855377E-4 60-61 1.7649203720875725E-4 3.1768566697576304E-4 0.0 0.7251881316870628 9.177585934855377E-4 62-63 1.7649203720875725E-4 3.3533487069663877E-4 0.0 0.8365899055732302 9.530570009272892E-4 64-65 1.7649203720875725E-4 3.529840744175145E-4 0.0 0.9531628961496144 9.530570009272892E-4 66-67 1.7649203720875725E-4 3.529840744175145E-4 0.0 1.084014092536187 9.530570009272892E-4 68-69 1.9414124092963296E-4 3.7063327813839024E-4 0.0 1.2324438958287518 9.530570009272892E-4 70-71 2.117904446505087E-4 3.8828248185926597E-4 0.0 1.3923103831324442 9.70706204648165E-4 72-73 2.117904446505087E-4 3.8828248185926597E-4 0.0 1.5577187204044916 0.0010060046120899163 74-75 2.117904446505087E-4 3.8828248185926597E-4 0.0 1.7300984931462846 0.001023653815810792 76-77 2.117904446505087E-4 4.059316855801417E-4 0.0 1.925333984706612 0.001023653815810792 78-79 2.117904446505087E-4 4.235808893010174E-4 0.0 2.1455783979394205 0.0010589522232525436 80-81 2.117904446505087E-4 4.235808893010174E-4 0.0 2.3793950488335818 0.0010589522232525436 82-83 2.117904446505087E-4 4.235808893010174E-4 0.0 2.623924766386315 0.0010589522232525436 84-85 2.117904446505087E-4 4.235808893010174E-4 0.0 2.8761848351687913 0.0010589522232525436 86-87 2.117904446505087E-4 4.5887929674276885E-4 0.0 3.153153789160495 0.0010766014269734192 88-89 2.117904446505087E-4 4.5887929674276885E-4 0.0 3.4480719833363276 0.001094250630694295 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2070 0.0 55.10858 1 GTATCAA 4180 0.0 44.60272 1 ATCAACG 4615 0.0 38.20286 3 TCAACGC 4545 0.0 38.111206 4 CAACGCA 4730 0.0 36.1262 5 TATCAAC 5080 0.0 35.403885 2 AACGCAG 5020 0.0 32.210194 6 GTACATG 5000 0.0 28.342804 1 ACGCAGA 5725 0.0 27.865763 7 TACATGG 5140 0.0 26.682783 2 CGCAGAG 5905 0.0 26.240488 8 ACATGGG 5485 0.0 23.077267 3 GCAGAGT 6545 0.0 22.350618 9 CATGGGG 4150 0.0 20.203928 4 GAGTACT 4335 0.0 19.260454 12-13 GTATATA 1365 0.0 19.144075 1 AGAGTAC 6145 0.0 19.075493 10-11 CAGAGTA 6425 0.0 18.96844 10-11 GAGTACA 3875 0.0 18.415365 1 GTACTTT 4720 0.0 18.045464 14-15 >>END_MODULE